KEY ENGINEERING MATERIALS
·
2016
·
IF 0
Ben Cang Cui,Jing Li,Hui Ning Wang,Yuan Hua Lin,Yang Shen,Ming Li,Ce Wen Nan
Polymer-infiltrated-feldspar used for restorative composite CAD/CAM blocks were prepared by infiltrating polymerizable monomers into the partially sintered feldspar blocks. The flexural strength was tested according to the standard of ISO-4049. The micro-structure was examined by SEM. The effects of sintering temperature, high pressure and the addition of SiO2 and Al2O3 on the ultimate flexural strength were evaluated. The flexural strength corresponding to infiltration with pressure was much higher than that without pressure at high sintering temperature. The addition of SiO2 and Al2O3 into the natural feldspar mineral increased the flexural strength of the blocks significantly.
研究领域:
材料科学
·
牙科医学
✓ 已验证
DOI
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Cai-Fang Liu,Xiao-Yan Ran,Cheng Liu,Yu-lan Zhu,Yan-Rui Lu,Yu-Ping Yang,Bang-Jin Wang,Jun-Hui Zhang,Sheng-Ming Xie,Li-Ming Yuan:
Chiral metal–organic frameworks, an emerging type of chiral porous materials, possess many outstanding characteristics such as high surface area, tunable pore sizes, and good chemical stability. Herein, we prepared a chiral [Zn 2 (D-Cam) 2 (4,4′-bpy)] n @SiO 2 core–shell composite on the SiO 2 -NH 2 microspheres by an in-situ growth method. The prepared [Zn 2 (D-Cam) 2 (4,4′-bpy)] n @SiO 2 -packed column exhibited high-resolution separation of racemic compounds and positional isomers at a low column backpressure (9–12 bar). Twelve pairs of enantiomers including alcohols, amines, epoxides, ketones, and esters were well resolved with sharp peak shapes on the [Zn 2 (D-Cam) 2 (4,4́-bpy)] n @SiO 2 chromatographic column. Compared the separation performance of [Zn 2 (D-Cam) 2 (4,4́-bpy)] n @SiO 2 -packed column and two chromatographic columns (commercial Chiralpak AD-H column and as-prepared SiO 2 -NH 2 column), the [Zn 2 (D-Cam) 2 (4,4′-bpy)] n -packed column and the commercial Chiralpak AD-H column have a good complementarity in chiral separation, and it also offered better separation performance compared to the SiO 2 -NH 2 column for separating positional isomers. The effects of column temperature and analyte mass on the HPLC separation were investigated. Additionally, the [Zn 2 (D-Cam) 2 (4,4́-bpy)] n @SiO 2 packed column has good repeatability and stability for HPLC separation of enantiomers and positional isomers. The relatvive standard deviations (RSDs) for repeated separations of 2,2,2-trifluoro-1-(9-anthryl)ethanol and o , m , p -bromoaniline were less than 0.8 % and 1.2 % for the retention time and peak area, respectively. After continuous use for one month, the RSDs for the retention time and peak area of them were in the range of 1.1–1.3 % and 1.5–1.8 % (inter-day and intra-day, n = 5), respectively. This work also indicates that the chiral MOFs@SiO 2 composites are promising for HPLC separation.
研究领域:
分析化学
·
材料科学
·
色谱技术
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 1
·22
·
IF 5.4
Xia Zheng,Xiaofei Liu,Zhuo Wang,Rui Li,Qiaoli Zhao,Bingbing Song,Kit-Leong Cheong,Jianping Chen,Saiyi Zhong
Chondroitin sulfate (CS), a class of glycosaminoglycans covalently attached to proteins to form proteoglycans, is widely distributed in the extracellular matrix and cell surface of animal tissues. In our previous study, CS was used as a template for the synthesis of seleno-chondroitin sulfate (SeCS) through the redox reaction of ascorbic acid (Vc) and sodium selenite (Na2SeO3) and we found that SeCS could inhibit tumor cell proliferation and invasion. However, its effect on angiogenesis and its underlying mechanism are unknown. In this study, we analyzed the effect of SeCS on tube formation in vitro, based on the inhibition of tube formation and migration of human umbilical vein endothelial cells (HUVECs), and evaluated the in vivo angiogenic effect of SeCS using the chick embryo chorioallantoic membrane (CAM) assay. The results showed that SeCS significantly inhibited the angiogenesis of chicken embryo urothelium. Further mechanism analysis showed that SeCS had a strong inhibitory effect on VEGFR2 expression and its downstream PI3K/Akt signaling pathway, which contributed to its anti-angiogenic effects. In summary, SeCS showed good anti-angiogenic effects in an HUVEC cell model and a CAM model, suggesting that it may be a potential angiogenesis inhibitor.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DENTISTRY
·
2017
·
IF 3.456
Bencang Cui,Jing Li,Huining Wang,Yuanhua Lin,Yang Shen,Ming Li,Xuliang Deng,Cewen Nan
Objective To fabricate indirect restorative composites for CAD/CAM applications and evaluate the mechanical properties. Methods Polymer-infiltrated-ceramic composites were prepared through infiltrating polymer into partially sintered sodium aluminum silicate ceramic blocks and curing. The corresponding samples were fabricated according to standard ISO-4049 using for mechanical properties measurement. The flexural strength and fracture toughness were measured using a mechanical property testing machine. The Vickers hardness and elastic modulus were calculated from the results of nano-indentation. The microstructures were investigated using secondary electron detector. The density of the porous ceramic blocks was obtained through TG-DTA. The conversion degrees were calculated from the results of mid-infrared spectroscopy. Results The obtained polymer infiltrated composites have a maximum flexural strength value of 214 ± 6.5 MPa, Vickers hardness of 1.76–2.30 GPa, elastic modulus of 22.63–27.31 GPa, fracture toughness of 1.76–2.35 MPa m 1/2 and brittleness index of 0.75–1.32 μm −1/2 . These results were compared with those of commercial CAD/CAM blocks. Our results suggest that these materials with good mechanical properties are comparable to two commercial CAD/CAM blocks. Conclusion The sintering temperature could dramatically influence the mechanical properties. Clinical significance Restorative composites with superior mechanical properties were produced. These materials mimic the properties of natural dentin and could be a promising candidate for CAD/CAM applications.
研究领域:
材料科学
·
牙医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Advanced Ceramics
·
2017
·Vol. 6
·期 1
·73-79
·
IF 1.198
Wang Huining,Cui Bencang,Li Jing,Li Shu,Lin Yuanhua,Liu Deping,Li Ming
A new type of polymer-infiltrated-ceramic-network composites (PICNs) was fabricated by infiltrating methacrylate-based monomers into partially sintered porous ceramics. The mechanical properties (flexural strength, flexural modulus, elastic modulus, Vickers hardness, fracture toughness) were investigated and compared with that of the natural tooth and common commercial CAD/CAM blocks. Our results indicated that sintering temperature and corresponding density of porous ceramics have an obvious influence on the mechanical properties, and PICNs could highly mimic the natural tooth in mechanical properties. The biocompatibility experiments evaluated through in vitro cell attachment and proliferation of BMSCs showed good biocompatibility. The mechanical properties and biocompatibility confirmed that PICN could be a promising candidate for CAD/CAM blocks for dental restoration.
研究领域:
材料科学
·
生物医学工程
·
牙科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yuandong Lin,Ji Ma,Da-Wen Sun,Jun-Hu Cheng,Chenyue Zhou
A fluorescent sensor array (FSA) combined with deep learning (DL) techniques was developed for meat freshness real-time monitoring from development to deployment. The array was made up of copper metal nanoclusters (CuNCs) and fluorescent dyes, having a good ability in the quantitative and qualitative detection of ammonia, dimethylamine, and trimethylamine gases with a low limit of detection (as low as 131.56 ppb) in range of 5 ∼ 1000 ppm and visually monitoring the freshness of various meats stored at 4 °C. Moreover, SqueezeNet was applied to automatically identify the fresh level of meat based on FSA images with high accuracy (98.17 %) and further deployed in various production environments such as personal computers, mobile devices, and websites by using open neural network exchange (ONNX) technique. The entire meat freshness recognition process only takes 5 ∼ 7 s. Furthermore, gradient-weighted class activation mapping (Grad-CAM) and uniform manifold approximation and projection (UMAP) explanatory algorithms were used to improve the interpretability and transparency of SqueezeNet. Thus, this study shows a new idea for FSA assisted with DL in meat freshness intelligent monitoring from development to deployment.
研究领域:
食品科学
·
化学传感
·
人工智能
·
机器学习
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Yihan Yu,Dandan Tian,Sanghyok Ri,Tongchol Kim,Kwangjin Ju,Jiongming Zhang,Shuangshuang Teng,Weixia Zhang,Wei Shi,Guangxu Liu
Although accumulating data demonstrated that gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter , plays an important regulatory role in immunity of vertebrates, its immunomodulatory function and mechanisms of action remain poorly understood in invertebrates such as bivalve mollusks . In this study, the effect of GABA on phagocytic activity of hemocytes was evaluated in a commercial bivalve species, Tegillarca granosa . Furthermore, the potential regulatory mechanism underpinning was investigated by assessing potential downstream targets. Data obtained demonstrated that in vitro GABA incubation significantly constrained the phagocytic activity of hemocytes. In addition, the GABA-induced suppression of phagocytosis was markedly relieved by blocking of GABA A and GABA B receptors using corresponding antagonists. Hemocytes incubated with lipopolysaccharides (LPS) and GABA had significant higher K + -Cl - cotransporter 2 (KCC2) content compared to the control. In addition, GABA treatment led to an elevation in intracellular Cl − , which was shown to be leveled down to normal by blocking the ionotropic GABA A receptor. Treatment with GABA A receptor antagonist also rescued the suppression of GABA A receptor-associated protein ( GABARAP ), KCC , TNF receptor associated factor 6 ( TRAF6 ), inhibitor of nuclear factor kappa-B kinase subunit alpha ( IKKα ), and nuclear factor kappa B subunit 1 ( NFκB ) caused by GABA incubation. Furthermore, incubation of hemocytes with GABA resulted in a decrease in cAMP content, an increase in intracellular Ca 2+ , and downregulation of cAMP-dependent protein kinase ( PKA ), calmodulin kinase II ( CAMK2 ), calmodulin ( CaM ), calcineurin ( CaN ), TRAF6 , IKKα , and NFκB . All these above-mentioned changes were found to be evidently relieved by blocking the metabotropic G-protein-coupled GABA B receptor. Our results suggest GABA may play an inhibitory role on phagocytosis through binding to both GABA A and GABA B receptors, and subsequently regulating corresponding downstream pathways in bivalve invertebrates.
研究领域:
免疫学
·
分子生物学
·
海洋生物学
·
无脊椎动物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Mei Zhang,Jun-Hui Zhang,Yan Zhang,Bang-Jin Wang,Sheng-Ming Xie,Li-Ming Yuan
This study focused on exploring the chromatographic characteristic of a homochiral MOF [Cu 2 ( d -Cam) 2 (4,4′-bpy)] n with 3-D six-connected self-penetrating architectures for the separations of positional isomers and chiral compounds. Some effects such as mobile phase composition, column temperature, and analytes mass for separations on this chiral column also have been investigated. Among thirteen tested racemates, six compounds, namely three alcohols, a diol, a naphthol and a ketone were successfully discriminated, and ten useful positional isomers which have never been separated on other MOFs stationary phases in HPLC except dichlorobenzene and cresol isomers, were also analyzed by this chiral stationary phase. The results indicated that [Cu 2 ( d -Cam) 2 (4,4′-bpy)] n exhibited good selectivity for positional isomers and enantiomers. Therefore, it is practicable that the separation of positional isomers and enantiomers are carried out on the chiral MOF column.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DENTAL MATERIALS JOURNAL
·
2022
·
IF 2.418
Xia WEI,Mingjun WANG,Yu PAN,Honglei LIN,Lei JIANG,Yinghui WANG,Hui CHENG
To investigate the influence of fabrication method on the biological properties of modified PEEK. Two materials were tested in this study: press and CAD/CAM milled ceramic-reinforced PEEK (BioHPP, Bredent, Germany). The residual diphenyl sulfone in these two materials was detected and their biological effects on human gingival fibroblasts (HGFs) were evaluated by assaying cell viability, apoptosis, and inflammation. Diphenyl sulfone was not detected in press and milled BioHPP. The cell viability of them complied with ISO standards. The mRNA expression levels of Bax and Caspase-3 were up-regulated and that of Bcl-2 was downregulated when HGFs were cultured with the eluates of these two materials. There was no difference in cell viability, apoptosis, and inflammation between them. Hence, the fabrication method did not affect the biological properties of modified PEEK, the press and CAD/CAM milled modified PEEK all presented favorable cytocompatibility despite the expression of apoptosis-associated genes at the mRNA level.
研究领域:
材料科学
·
生物医学工程
·
细胞生物学
·
牙科材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Therapy
·
2024
·
IF 3.4
Shule Zhang,Dong Li,Linghong Liu,Qing Shi,Xiuli Ju
Introduction It is essential to acknowledge that the cardiovascular toxicity associated with anthracycline drugs can be partially attributed to the damage inflicted on blood vessels and endothelial cells. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have the potential to repair cellular processes and promote tissue regeneration through the transfer of signaling molecules such as miRNAs. In the present study, we investigated the effects of MSC-EVs on daunorubicin (DNR)-damaged human cardiac microvascular endothelial cells (HCMEC) and developing blood vessels of Chicken Chorioallantoic Membrane (CAM) in vivo. Materials and methods We constructed in vitro and in vivo models of DNR-damaged endothelial cells and developing blood vessel. Scratch wound assays, EdU assays, tube formation assays, and SA-β-Gal staining were used to evaluate the effects of MSC-EVs on cell migration, proliferation, angiogenesis capacity and cell senescence. Blood vessel area was used to assess the effects of MSC-EVs on CAM vasculature. RT-qPCR was used to detect the mRNA expression levels of inflammatory molecules. RNA sequencing was employed to compare differential gene expression and downstream regulatory mechanisms. RNA interference experiments and miRNA mimic overexpression experiments were used to validate the regulatory effects of target genes and downstream signaling pathways. Results We found that MSC-EVs improved the migration, proliferation, and angiogenesis of HCMEC, while also alleviating cellular senescence. The angiogenic effect on the developing blood vessels was confirmed in vivo. We identified that MSC-EVs downregulated the expression of PARP9, thereby inhibiting the STAT1/pSTAT1 signaling pathway. This downregulation effect is likely mediated by the transfer of miR-186-5p from MSC-EVs to HCMEC. Overexpression of miR-186-5p in DNR-damaged HCMEC also exhibited the aforementioned downregulation effect. In vivo, the introduction of miR-186-5p mimics enhanced angiogenesis in the CAM model. Conclusions To summarize, our study reveals that MSC-EVs can restore the cellular function of DNR-damaged HCMEC and alleviate cellular senescence through the miR-185-5p-PARP9-STAT1/pSTAT1 pathway. This finding highlights the potential of MSC-EVs as a therapeutic strategy for mitigating the detrimental effects of anthracycline-induced endothelial damage.
研究领域:
心血管毒性
·
干细胞治疗
·
分子生物学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Fei Sun,Yi Lu,Zhihao Wang,Boyou Zhang,Zhiming Shen,Lei Yuan,Cong Wu,Qiang Wu,Wenlong Yang,Guozhong Zhang,Ziyin Pan,Hongcan Shi
Tissue engineering technology provides effective alternative treatments for tracheal reconstruction. The formation of a functional microvascular network is essential to support cell metabolism and ensure the long-term survival of grafts. However, given the lack of an identifiable vascular pedicle of the trachea that could be anastomosed to the blood vessels directly in the recipient's neck, successful tracheal transplantation faces significant challenges in rebuilding the adequate blood supply of the graft. Herein, we describe a one-step method to construct microvascularization of tissue-engineered trachea in orthotopic transplantation. Forty rabbit tracheae were decellularized using a vacuum-assisted decellularization (VAD) method. Histological appearance and immunohistochemical (IHC) analysis demonstrated efficient removal of cellular components and nuclear material from natural tissue, which was also confirmed by 4′-6-diamidino-2-phenylindole(DAPI) staining and DNA quantitative analysis, thus significantly reducing the antigenicity. Scanning electron microscopy (SEM), immunofluorescence (IF) analysis, GAG and collagen quantitative analysis showed that the hierarchical structures, composition and integrity of the extracellular matrix (ECM) were protected. IF analysis also demonstrated that basic fibroblast growth factor (b-FGF) was preserved during the decellularization process, and also exerted biocompatibility and proangiogenic properties by the chick chorioallantoic membrane(CAM) assay. Xenotransplantation assays indicated that the VAD tracheal matrix would no longer induced inflammatory reactions implanted in the body for 4 weeks after treated by VAD more than 16 h. Furthermore, we seeded the matrix with bone marrow-derived endothelial cells (BMECs) in vitro and performed in vivo tracheal patch repair assays to prove the biocompatibility and neovascularization of VAD-treated tracheal matrix, and the formation of a vascular network around the patch promoted the crawling of surrounding ciliated epithelial cells to the surface of the graft. We conclude that this natural VAD tracheal matrix is non-immunogenic and no inflammatory reactions in vivo transplantation. Seeding with BMECs on the grafts and then performing orthotopic transplantation can effectively promote the microvascularization and accelerate the native epithelium cells crawling to the lumen of the tracheal graft.
研究领域:
组织工程
·
移植学
·
再生医学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2024
·
IF 2.6
Zijian Wang,Xixi Song,Yanjun Wei,Xianggen Wu,Ying Jie
This experiment aimed to investigate the feasibility of cytisine (CYT) in treating eye diseases with ocular topical application. An in vitro cytotoxicity test, a hen’s egg test-chorioallantoic membrane (HET-CAM), and a mouse eye tolerance test were used to fully reveal the ocular safety profiles of CYT. For the efficacy evaluations, CYT’s effects on cell wound healing, against H2O2-induced oxidative stress damages on cells, and on benzalkonium chloride (BAC)–induced dry eye disease (DED) in mice were evaluated. Results showed that CYT did not show any cytotoxicities at concentrations no higher than 250 μg/ml, while lipoic acid (α-LA) at 250 μg/ml and BAC at 1.25 μg/ml showed significant cytotoxicities within 48 h incubation. The HET-CAM and mouse eye tolerance test confirmed that 0.5% CYT eye drops demonstrated good safety characteristics. Efficacy evaluations showed that CTY significantly promoted cell migration and wound healing. CYT significantly improved cell survival against H2O2-induced oxidative stress damage by reversing the imbalance between the reactive oxygen species (ROS) and antioxidant defense mechanisms. The animal evaluation of the BAC-induced dry eye model revealed that CYT demonstrated a strong treatment effect, including reversing ocular surface damages, recovering corneal sensitivity, and inhibiting neovascularization; HMGB1/NF-κB signaling was involved in this DED treatment by CTY. In conclusion, CYT had strong experimental treatment efficacy against DED with good ocular safety profiles, and it might be a novel and promising drug for DED.
研究领域:
眼科学
·
药理学
·
细胞生物学
·
实验医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）