MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 3
·547-555
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IF 3.719
Liu Jinshui,Liu Guoning,Zang Lingjie,Liu Wenxiu
Calcein was first covalently grafted onto the surface of magnetic silica nanoparticles to obtain a novel fluorescent nanoprobe for sensitive and selective determination of Cu(II) ion. The nanoparticles were synthesized in a two-step reaction under mild conditions and characterized using small-angle X-ray scattering, Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The nanoprobe also displays good selectivity over other metal ions and is highly stable under conditions of high ionic strength. The response to Cu(II) ranges from 80 nM to 1.25 μM. The detection limit (LOD) is 43 nM which is lower than previously reported LODs. The probe can be regenerated by adding the complexing agent EDTA and also can be separated from a sample mixture by applying a magnetic field. We conclude that this method represents a new and convenient approach to the development of magnetic and reusable optical probes.
Calcein was first covalently grafted onto the surface of magnetic silica nanoparticles to obtain a novel fluorescent nanoprobe for sensitive and selective determination of Cu(II) ion. The nanoprobe displays good selectivity over other metal ions and is highly stable under conditions of high ionic strength.
研究领域:
分析化学
·
材料科学
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DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Yuxiao Chen,Lei Zhang,Juhong Miao,Jingfa Li
Dye-sensitized halide perovskite systems have recently been demonstrated as promising candidates for photoelectrochemical devices. In this manuscript, we focus on the calcein dye-sensitized halide perovskite systems for aqueous-based photoelectrochemical system. This is achieved via the deposition of the commercially available self-assembled dye layer at the CH 3 NH 3 PbI 3 /TiO 2 interface. Enhanced optoelectronic properties are obtained when the calcein dye is incorporated in the perovskite-based material. The photoelectrode consisting of the calcein/CH 3 NH 3 PbI 3 hybrid film produces a photocurrent value that is orders of magnitude higher than that from a bare CH 3 NH 3 PbI 3 photoelectrode under the same condition. In addition, the calcein dye improves the light-emitting properties of the CH 3 NH 3 PbI 3 -based photoelectrode in water. The first-principles calculations suggest that multiple Lewis-acid-base coordination sites are available between the calcein dye and the halide perovskite substrate, such that the calcein dye could simultaneously enhance the light harvesting properties and passivates the perovskite electrode. This manuscript highlights the potentials of the multifunctional dye-sensitized halide perovskite materials, and facilitates the convergent understanding of halide perovskite materials for integrated optoelectronic devices.
研究领域:
材料科学
·
化学
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DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 31
·3933-3943
·
IF 2.596
Qiuyue Yin,Maiqian Nie,Zhenjun Diwu,Yuting Zhang,Lei Wang,Dandan Yin,Liancheng Li
A rapid method for readily detecting the total numbers of viable bacterial cells in numerous samples (including surface water, solid inoculants, and soil samples) is reported using a newly developed hand-held fluorometer and a fluorescent dye Calcein UltraGreen™ AM. Compared to the traditional plate counting method that requires 48 hours of cultivation, the newly established method does not require any incubation time, making the detection method faster and more convenient. The portable rapid detection fluorometer has a wide dynamic range of relative fluorescence intensity from 45 to 30 133. It can detect bacterial concentration ranging from 105 to 1010 cells per mL. This newly established method has good applicability for accurately and quickly detecting the cell number of viable bacteria in various samples. The results of the fluorescence-based method were compared with those of the traditional plate counting method, and it was found that the relative standard deviation was less than 6%. This new rapid measurement system provides a robust method for the rapid on-site detection of viable bacteria.
研究领域:
微生物学
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环境科学
·
分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 2.6
Bingyu Fu,Yanru Shao,Zhixiong Zheng,Shengxin Wen,Yingli Guo,Qi Xu,Hualin Yang
In this study, a simple and sensitive dual-mode biosensor was designed based on the color of 2,6-dichloroindiphenol (DCIP) and the fluorescence of calcein. The target alkaline phosphatase (ALP) can catalyze the dephosphorylation of L-ascorbic acid-2-phosphate (AAP) to form ascorbic acid (AA) and phosphate ions. The reducing properties of AA can reduce the DCIP from blue to colorless. Meanwhile, phosphate ions can chelate with europium ions (Eu 3+ ) to restore the fluorescence of Eu 3+ -Calcein. In colorimetric mode, the linear detection range for ALP was 1.56-50 U/L with a limit of detection (LOD) of 0.27 U/L. In fluorescence mode, the linear detection range was 0.78-25 U/L and LOD was 0.23 U/L. Besides, the selectivity of this dual-mode for ALP was very excellent and the recovery rates in serum samples were also satisfactory.
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DOI
PubMed
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2014
·
IF 2.576
Zhi-Wei Liu,Xin-An Zeng,Da-Wen Sun,Zhong Han
Effects of PEF on permeabilization of calcein-filled large unilamellar vesicles (LUV) with average diameter of 1705.64 ± 55.91 nm and small unilamellar vesicles (SUV) with average diameter of 213.06 ± 5.50 nm, suspended in an isosmotic sucrose solution (150 mM) were investigated. The suspensions of vesicles were exposed to PEF at various electric field strengths in the interval of 0–50 kV/cm and different treatment times ranging from 0 to 4.8 ms. The intensity of permeabilization induced by PEF was analyzed in terms of the changes of fluorescence intensity of the suspension solution and the average diameter of vesicles, due to the leakage of self-quench calcein encapsulated in vesicles. Results indicated that the permeability of LUV was increased with increasing intensity of electric field (>10 kV/cm) and treatment time, and the release percentage of calcein ( R value) was increased by 57.98% (from 0.46% to 58.44%) with the increase of the electric field strength (from 10 to 50 kV/cm) at 4.8 ms. However, the R value was only increased by 14.62% (from 43.82% to 58.44%) with the increase of the treatment time (from 1.2 to 4.8 ms) at 50 kV/cm. Average diameter of LUV was sharply decreased from 1705.64 ± 55.91 to 465.60 ± 37.12 nm with the maximum value at 50 kV/cm and 4.8 ms. Results also showed that shrinkage or rupture of LUV happened, as confirmed by the change of size distribution of LUV after treatment by PEF. However, as for SUV, the changes of the permeability of vesicles and average diameter were not observed. In addition, it was demonstrated that the magnitude of the induced transmembrane potential was proportional to the radius of the vesicle, and larger vesicles could be polarized or ruptured at relatively lower field strength than smaller vesicles.
研究领域:
生物物理学
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食品科学与工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jin Yang,Xin Shu,Shangying Qin,Li Huang,Shiqi Cheng,Yilin Wang
Co 3 O 4 nanoclusters (NCs) was prepared, and a novel method based on hydrogen peroxide (H 2 O 2 ) mediated fluorescence recovery of calcein-Co 3 O 4 NCs system for cholesterol detection was developed. The detection utilizes fluorescence quenching and recovery of calcein. In the absence of H 2 O 2 , the fluorescence of calcein is quenched by Co 2+ ions on the surface of Co 3 O 4 NCs. However, the fluorescence of calcein is restored in the presence of H 2 O 2 as a result of the oxidation of Co 2+ ions to Co 3+ ions. Further investigation indicates tha Co 3 O 4 t there is a correlation between H 2 O 2 concentration and fluorescence enhancement of calcein. Due to H 2 O 2 being one of the products of cholesterol catalytic oxidation, a novel cholesterol fluorescence measurement method has been established, which can achieve sensitive detection of cholesterol in the range of 1.0–50 μM, with a limit of detection (LOD) as low as 0.48 μM. More importantly, the feasibility of the method was verified in human serum total cholesterol determination with satisfactory results, indicating its potential application in real sample analysis.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 6
·976
·
IF 5.719
Haiyan Qi,Qiuying Li,Jing Jing,Tao Jing,Chuntong Liu,Lixin Qiu,Rokayya Sami,Mahmoud Helal,Khadiga Ahmed Ismail,Amani H. Aljahani
In our study, a unique ratiometric fluorescent sensor for the rapid detection of arginine (Arg) and acetaminophen (AP) was constructed by the integration of blue fluorescent N-CDs and yellowish-green fluorescent calcein. The N-CD/calcein ratiometric fluorescent sensor exhibited dual emission at 435 and 519 nm under the same excitation wavelength of 370 nm, and caused potential Förster resonance energy transfer (FRET) from N-CDs to calcein. When detecting Arg, the blue fluorescence from the N-CDs of the N-CD/calcein sensor was quenched by the interaction of N-CDs and Arg. Then, the fluorescence of our sensor was recovered with the addition of AP, possibly due to the stronger association between AP and Arg, leading to the dissociation of Arg from N-CDs. Meanwhile, we observed an obvious fluorescence change from blue to green, then back to blue, when Arg and AP were added, exhibiting the “on–off–on” pattern. Next, we determined the detection limits of the N-CD/calcein sensor to Arg and AP, which were as low as 0.08 μM and 0.02 μM, respectively. Furthermore, we discovered that the fluorescence changes of the N-CD/calcein sensor were only responsible for Arg and AP. These results suggested its high sensitivity and specificity for Arg and AP detection. In addition, we have successfully achieved its application in bovine serum samples, indicating its practicality. Lastly, the logic gate was generated by the N-CD/calcein sensor and presented its good reversibility. Overall, we have demonstrated that our N-CD/calcein sensor is a powerful sensor to detect Arg and AP and that it has potential applications in biological analysis and imaging.
研究领域:
生物化学
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分析化学
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医学检测
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 33
·期 3
·965-972
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IF 2.525
Zheng Yabin,Li Chen,Li Quan,Zhang Taoyi,Chen Jing,Ji Wenxi,Wei Yun
Sodium tripolyphosphate, a food additive, is applied broadly in food industry. However, excessive accumulation of sodium tripolyphosphate can result in electrolyte abnormality of the body. Therefore, it is of great importance to investigate an effective method for the detection of sodium tripolyphosphate. In this work, nitrogen-doped carbon dots (NCDs) with constant fluorescence were fabricated using a domestic microwave oven. A ratiometric fluorescent probe was constructed in which NCDs were as internal standard, calcein & Eu 3+ were as the detection signal. The fluorescence of calcein at 515 nm was quenched by Eu 3+ , whereas the emission peak of NCDs at 446 nm was almost unchanged. Additionally, the fluorescence of calcein was recovered because of the strong interaction of sodium tripolyphosphate and Eu 3+ . The linear range for sodium tripolyphosphate was 0.5-6 µmol/L with detection limit of 0.12 µmol/L. Furthermore, the ratiometric fluorescent probe was applied for sodium tripolyphosphate detection in real milk samples.
研究领域:
分析化学
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食品科学
·
材料科学
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Xiaoling Guo,Jinlian Li,Jiwen Cui,Yuhua Wang,Hongfu Gao,Sue Hao,Dongmei Wu
It is highly desirable to develop cost-effective and sensitive cell sensors to evaluate cytotoxicity of anticancer drugs. For the first time, a simple and cheap poly calcein modified electrode(PCA/GCE) prepared by one-step in situ polymerization was applied for study the electrochemical behavior of MCF-7 cells. Since the negative charge and unique properties of calcein can increase sensitivity, the resulting linear scanning of the MCF-7 cells on electrode exhibited two anodic peak that was ascribe to xanthine/guanine(signal) and hypoxanthine/adenine (signal). Meanwhile, the nonlinear exponent relationships between peak current (I pa ) and cell concentration (C) in the wide concentration range were firstly demonstrated. Two independent index equation based on two signal were I pa (signal) = 7.458 − 6.415e (-C/52380) (R 2 = 0.986) and I pa (signal) = 4.023 − 3.773e (-C/228700) (R 2 = 0.984) with the low detection limit of 3000 cells mL -1 , respectively. Electrochemical responses based on two signals of MCF-7 cells was applied to evaluate cytotoxicity effect of cyclophosphamide (CTX) on MCF-7 cells, the result was in good agreement with that of MTT method. This proposed method could be used to evaluate cytotoxicity of drug.
研究领域:
生物医学工程
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肿瘤学
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药物分析
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Xiaotao Liu,Shanshan Xing,Yuanteng Xu,Ruiqing Chen,Chang Lin,Liangqia Guo
Graphitic carbon nitride (g-C 3 N 4 ) has been shown as a promising visible-light photosensitizer for photodynamic therapy (PDT) application. Nevertheless, its therapeutic efficiency is limited by the low efficiency of visible-light utilization. To overcome this issue, 3-amino-1,2,4-triazole-derived graphitic carbon nitride nanosheets (g-C 3 N 5 NSs) are prepared for PDT application. The addition of nitrogen-rich triazole group into the g-C 3 N 4 motif significantly makes the light absorption of g-C 3 N 5 NSs red-shift with the band gap down to 1.95 eV, corresponding to a absorption edge at a wavelength of 636 nm. g-C 3 N 5 NSs generate superoxide anion radicals (O 2 •– ) and singlet oxygen ( 1 O 2 ) under the irradiation of a low-intensity white light emitting diode. Owing to the high efficiency of visible-light utilization, g-C 3 N 5 NSs show about 9.5 fold photocatalytic activity of g-C 3 N 4 NSs. In vitro anticancer studies based on the results of CCK-8 assay, Calcein-AM/PI cell-survival assay and photo-induced intracellular ROS level analysis in living HeLa cells demonstrate the potential of g-C 3 N 5 NSs as a low-toxic and biocompatible high-efficient photosensitizer for PDT.
研究领域:
材料科学
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纳米技术
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光动力治疗
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癌症研究
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Xue Mei,Wen Xiang,Wenyan Pan,Quan Lin,Xueyan Jia,Xuan Zhang,Xue Tang,Xiangrong Cheng,Yuyan Weng,Kai Yang,Naiyan Lu
Background: In acute liver injury (ALI), cell membrane damage could induce an inflammatory response and oxidative stress. As a membrane glycerophospholipid, plasmalogens (PLS) are crucial in regulating the cell membrane properties and exhibit beneficial effects in various liver diseases. However, the specific regulatory effects of PLS in the ALI remain unknown. Methods: We utilized CCl4 to induce ALI in AML12 hepatocytes and C57BL/6J mice and examined oxidative stress indicators and inflammatory cytokine levels. Our study further validated the effect of PLS on cell membrane integrity by Lactate Dehydrogenase (LDH) release assay and Dil/Calcein assay, and molecular dynamics (MD) simulations were employed to elucidate the molecular mechanisms by which PLS affected cell membranes. Results: PLS attenuated hepatocyte damage both in vivo and in vitro. Moreover, PLS increased levels of SOD, GSH, and CAT and inhibited the production of malondialdehyde. PLS succeeded in decreasing proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while increasing anti-inflammatory cytokines (IL-10). Furthermore, PLS effectively maintained the cell membrane integrity. The MD simulations well explained the molecular mechanisms: a high level of PLS modulated the cell membrane properties, enabling them to be more flexible, elastic, and less prone to rupture. Conclusions: Our study illustrated the effect and molecular mechanisms of PLS against ALI, potentially broadening its application in liver diseases.
研究领域:
药理学
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毒理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）