BIORESOURCE TECHNOLOGY
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2017
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IF 5.651
Zheng-Zhe Zhang,Jia-Jia Xu,Zhi-Jian Shi,Yu-Hui Bai,Ya-Fei Cheng,Hai-Yan Hu,Ren-Cun Jin
Given the increasing use of nanoscale zero-valent iron (NZVI) particles for environmental remediation and wastewater treatment, their potential impact on anaerobic ammonium oxidation (anammox) bacteria was investigated in this study using anammox sludge. Batch assays showed that NZVI concentrations up to 200 mg L −1 did not affect anammox activity, reactive oxygen species production, and cell membrane integrity. The nitrogen removal efficiency of the continuous-flow reactor fluctuated in the presence of 20 or 50 mg L −1 NZVI, but it could return to normal over time, even at 200 mg L −1 NZVI. 16S rDNA-based high-throughput sequencing indicated that although the presence of 10, 20, 50, and 200 mg L −1 NZVI to some extent affected microbial composition, the anammox bacteria ( Candidatus Kuenenia ) never lost its dominance. The abundance of gene families that are related to the assimilation and utilization of iron was down-regulated in response to the stress of high-level NZVI.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Jiajia Xu,Chao Li,Nanwen Zhu,Yanwen Shen,Haiping Yuan
The nitrite (NO 2 − ) inhibition in anaerobic ammonium oxidation (anammox) process is widely reported. Here, the effects of three pyrolytic biochars (CS300, CS550 and CS800) were investigated to alleviate NO 2 − stress on anammox process under exposure of varied NO 2 − -N concentrations (70, 200, 400 and 600 mg L −1 ). No nitrite inhibition was observed at 70 mg N L −1 . However, the total nitrogen removal efficiency (TNREs) decreased with NO 2 − -N concentration increased, while the biochar-amended groups achieved higher TNREs than the control (CK). At 200 mg N L −1 , the TNREs were 60.2%, 99.0%, 98.5% and 86.6% for CK, CS300, CS550 and CS800, respectively. At 400 mg N L −1 , the TNREs were 23.3%, 56.0%, 37.1% and 29.7% for CK, CS300, CS550 and CS800, respectively. At 600 mg N L −1 in which severe inhibition was observed, the TNREs were increased by 231% ( p = 0.002), 149% ( p = 0.014), and 51.0% ( p = 0.166) for CS300, CS550 and CS800, respectively, as compared to CK, with the corresponding specific anammox activity increased by 3.1-, 2,0- and 1.1-folds, respectively. CS300 enriched the relative abundance of Candidatus Kuenenia and increased the gene copies of functional genes ( hzs A, hdh , nir S and nir K). Besides, CS300 effectively alleviated the suppression of three membrane-associated enzyme complexes for anammox electron transport chain, indicating the possible contribution of redox-active moieties of CS300 to energy conversion metabolism for mitigating the NO 2 − -N inhibition. This study provided an effective strategy for alleviating NO 2 − -N stress by applying an environmentally compatible material (biochar) on anammox process.
研究领域:
环境科学
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生物技术
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废水处理
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2019
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IF 7.65
Yongpeng Ma,Denghui Wei,Xiaojing Zhang,Haoqiang Fu,Tao Chen,Jinping Jia
Anaerobic ammonium oxidation (Anammox) was an innovative process for nitrogen removal. In this study, CuO nanoparticles (NPs) was step-wise increasingly added to an MBR-based partial nitrification system, to investigate its feasibility for inducing Anammox and establishing autotrophic nitrogen removal system. Results showed that when CuO NPs was elevated to 5 mg L −1 , Anammox was successfully induced. The relative abundance of Nitrosomonas reached 13.73% while Candidatus Kuenenia increased to 4.79% from 0.46%, these two bacteria cooperatively contributed to the autotrophic nitrogen removal and improved the nitrogen removal rate (NRR) to 0.56 kg m -3 d −1 in 20 mg L −1 NPs. However, 50 mg L −1 NPs deeply suppressed the functional bacteria and decreased NRR to 0.14 kg m -3 d −1 . Finally, the NPs removal, transformation and adsorption in the system were evaluated. It was concluded that CuO NPs in low concentration (5 mg L −1 ) was effective for inducing Anammox and contributed to the survival of Anammox bacteria. The mechanism for inducing Anammox was attributed to the aggregation of CuO NPs which enabled the attached growth of AAOB as well as the suitable survival condition supplied by MBR.
研究领域:
环境工程
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微生物学
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水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）