ENVIRONMENTAL TOXICOLOGY
·
2013
·Vol. 29
·期 12
·1460-1470
·
IF 2.708
Mingbao Feng,Ruijuan Qu,Ying Li,Zhongbo Wei,Zunyao Wang
Hexabromobenzene (HBB) is a novel brominated flame retardant (BFR) with ample evidence of its ubiquitous existence in the aquatic ecosystems. However, to date, the toxicological effects of this BFR on fish have been inadequately researched. The present study was conducted, based on an in vivo model, to investigate HBB-induced biochemical changes in liver and gill tissues of Carassius auratus after medium-term exposure to different concentrations (10, 150, and 300 mg/kg) for 7, 14, and 25 days. Oxidative stress was evoked evidently for the prolonged exposure, demonstrated by significant inhibition in antioxidant enzymes activities including superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and glutathione S-transferase, and a decrease in reduced glutathione level, as well as simultaneous elevation in malondialdehyde content. Moreover, Na + , K + -ATPase activity, and protein level were remarkably reduced in fish tissues. Based on the integrated biomarker response, the toxic potency in each treatment was distinguished, and the more severe stress was mainly noted with the increasing concentrations and the extending durations. It was also observed that liver exhibited more pronounced alterations in biochemical parameters than gill, probably indicating the vulnerability of liver to HBB-triggered oxidative stress. Taken together, the results of this study clearly showed that HBB was capable of inducing oxidative stress and inhibiting Na + , K + -ATPase activity in different tissues of C. auratus after medium-term exposure. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1460–1470, 2014.
研究领域:
环境毒理学
·
水生生态学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Senyue Liu,Ming Xu,Baipeng Chen,Fulong Li,Yongqiang Deng,Yufan Zhang,Gang Lin,Defang Chen,Yi Geng,Yangping Ou,Xiaoli Huang
Because of the low host specificity , Ichthyophthirius multifiliis ( Ich ) can widely cause white spot disease in aquatic animals, which is extremely difficult to treat. Prior research has demonstrated a considerable impact of concentrated mannan-oligosaccharide (cMOS) on the prevention of white spot disease in goldfish , but the specific mechanism is still unknown. In this study, transcriptome sequencing, histological analysis, immunofluorescence analysis, phagocytosis activity assay and qRT-PCR assay were used to systematically reveal the potential mechanism of cMOS in supporting the resistance of goldfish ( Carrasius auratus ) to Ich invasion. According to the transcriptome analysis, the gill tissue of goldfish receiving the cMOS diet showed greater expression of mannose-receptor (MRC) related genes, higher phagocytosis activity, up-regulated expression of phagocytosis-related genes and inflammatory-related genes compared with the control, indicating that cMOS can have an effect on phagocytosis and non-specific immunity of goldfish. After the Ich challenge, transcriptome analysis revealed that cMOS fed goldfish displayed a higher level of phagocytic response, whereas non-cMOS fed goldfish displayed a greater inflammatory reaction. Besides, after Ich infection, cMOS-fed goldfish displayed greater phagocytosis activity, a stronger MRC positive signal, higher expression of genes associated with phagocytosis ( ABCB2 , C3 , MRC ), and lower expression of genes associated with inflammation ( IL-1β, IL-17, IL-8, TNF-α, NFKB ). In conclusion, our experimental results suggest that cMOS may support phagocytosis by binding to MRC on the macrophage cell membrane and change the non-specific immunity of goldfish by stimulating cytokine expression. The results of this study provide new insights for the mechanism of cMOS on parasitic infection, and also suggest phagocytosis-related pathways may be potential targets for prevention of Ich infection.
研究领域:
水产养殖
·
免疫学
·
寄生虫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Xiaoli Huang,Senyue Liu,Fengyuan Zuo,Lin Luo,Defang Chen,Yangping Ou,Yi Geng,Yufan Zhang,Gang Lin,Shiyong Yang,Wei Luo,Lizi Yin,Zhi He
Methods of supporting mucosal immune barrier integrity and prevention of some pathogenic infections in aquatic species , are key areas of active study, often focusing on feed additives . The objectives of this study were to explore the effects of feeding cMOS (concentrated mannan oligosaccharide) on the gill and skin mucosal barriers of goldfish ( Carassius auratus Linnaeus ) and evaluate health status during Ichthyophthirius multifiliis infection. After feeding the cMOS-containing diet for 60 days, Hematoxylin and eosin (H&E) staining showed greater length of gill lamella and thicker dermal dense layer, while Alcian Blue and Periodic acid–Schiff (AB-PAS) staining showed higher numbers of mucin cells in cMOS fed fish. Chemical analysis showed that fish fed cMOS had greater enzyme activity of lysozyme (LZM) and alkaline phosphatase (AKP) in gill and skin tissues, while qRT-PCR revealed higher expression of Muc-2 and IL-1β , as well as lower expression of IL-10 . After Ichthyophthirius multifiliis challenge, goldfish fed the cMOS diet had lower mortality and infection rates, as well as fewer visible white spots on the body surfaces. Histologically, the gill and skin of these fish presented less tissue damage and fewer parasites, and had a greater number of mucus cells. In addition, the expression of Muc-2 and IL-10 were notably higher while the expression of IL-1β was significantly lower in cMOS fed goldfish than control fed fish. In this study, cMOS fed goldfish had stronger immune barrier function of skin and gill mucous, and better survival following Ichthyophthirius multifiliis infection.
研究领域:
水生生物学
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免疫学
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渔业科学
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兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Yipeng Gu,Kai Chen,Bingwen Xi,Jun Xie,Xuwen Bing
Paeonol, a naturally occurring polyphenol isolated from medical plant , has been known to exhibit anti-oxidative and anti-inflammatory effects. In order to evaluate the effect of paeonol on Carassius auratus gibelio infected by pathogenic bacteria Aeromonas hydriphila . 750 fish were randomly divided into 5 groups, which separately treated with 0.85% sterile saline (blank), A. hydriphila (negative control), A. hydriphila with paeonol (4 mg/kg, 64 mg/kg), and A. hydriphila with enrofloxacin (12 mg/kg, positive control). Fish were anaesthetized with MS-222 (100 mg/L), and samples were collected at 6 and 72 h after A. hydriphila challenge. The results showed that compared with the negative group, the survival in paeonol groups marked increased by 14.75% and 18.94%. The plasma immunoglobulin M (IgM) was notably increased, and low density lipoprotein (LDL) was significantly decreased in paeonol groups at 6 h ( P < 0.05). The antioxidative enzymes catalase (CAT), total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) were significantly increased in paeonol groups at 6 h, while malondialdehyde (MDA) and myeloperoxidase (MPO) contents were lower ( P < 0.05). The inflammatory related genes MyD88 and TLR-5 were significantly downregulated, and the TLR-3 was significantly increased in paeonol groups at 72 h ( P < 0.05). In addition, histopathological analyses showed that the lesion in liver, spleen and caudal kidney were considerably attenuated in paeonol groups. In conclusion, paeonol could increase the survival rate, mitigate oxidative damage, inflammation, tissue lesions, and improve the immunity of gibel carp challenged with A. hydrophila .
研究领域:
水产养殖
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免疫学
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微生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Yingjie Liu,Zhongxiang Chen,Shanwei Li,Lu Ding,Xiaofeng Wei,Shicheng Han,Peng Wang,Yanchun Sun
Di-(2-ethylhexyl) phthalate (DEHP) is one of the most extensively utilized plasticizers in the plastic manufacturing process. It is widely used in various fields due to its low cost and excellent effect. Although there is evidence that DEHP is harmful to animal and human health, DEHP-induced gill toxicity in aquatic organisms is inconclusive, and its mechanism has not been fully elucidated. Here, we investigated the effects of DEHP acute exposure on crucian carp gills at environmentally relevant concentrations of 20, 100, and 500 μg/L. Multi-omics profiling and biochemical assays were employed to characterize the potential toxicological mechanisms. The results showed that acute exposure to 100 and 500 μg/L of DEHP leads to oxidative stress in gills, as evidenced by overproduction of reactive oxygen species (ROS), increased antioxidant enzyme activity , and the transformation of glutathione from reduced to oxidized form, resulting in lipid peroxidation. Integrative analysis of transcriptomics and metabolomics indicated that increased purine metabolism was the potential source of increased ROS. Moreover, lipid metabolism disorder, including arachidonic acid metabolism, induces inflammation. Further, DEHP causes the imbalance of the CYP enzyme system in the gill, and DEHP-induced gill toxicity in crucian carp was associated with interference with CYP450 homeostasis . Taken together, this study broadens the molecular understanding of the DEHP-induced gill toxicity in aquatic organisms and provides novel perspectives for assessing the effects of DEHP on target and non-target aquatic organisms in the environment.
研究领域:
环境毒理学
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水生生物学
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Wei Shi,Shuge Sun,Yu Han,Yu Tang,Weishang Zhou,Xueying Du,Guangxu Liu
Recently, the ubiquitous presence of microplastics (MPs) has drawn worldwide concern over its potential threat to aquatic organisms. However, the effects of MPs on the olfactory ability of fish and the subsequent odorant evoked behaviors remain elusive. In the present study, we analyzed the potential olfactory toxicity of polystyrene (PS) MPs by assessing olfactory-driven behaviors of goldfish in response to odorants. Our results showed that the olfactory-driven behavioral responses of goldfish to L -cysteine and taurocholic acid were significantly hampered by a 28-day MP exposure. Further analysis demonstrated that exposure to MPs may suppress the expression of genes encoding olfactory G protein-coupled receptors, inhibit the enzyme activities of cation transport ATPases crucial for action potential generation, alter the in vivo contents of neurotransmitters as well as metabolites involved in the transduction of electrical signals, and cause olfactory bulb injury and neurotoxicity closely related to the processing of electrical signals. In conclusion, the results obtained in the present study suggest that MPs at environmentally relevant concentrations could impair the olfactory-mediated behavioral responses of goldfish, probably through hampering odorant identification, action potential generation, olfactory neural signal transduction, and olfactory information processing.
研究领域:
环境科学
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水生毒理学
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鱼类生物学
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Lingzheng Lu,Conghui Shan,Difei Tong,Yingying Yu,Weixia Zhang,Xunyi Zhang,Yang Shu,Weifeng Li,Guangxu Liu,Wei Shi
Tetrabromobisphenol A (TBBPA) is one of the most extensively used brominated flame retardants and its increasing use in consumer products has raised concerns about its ecotoxicity. Given the ubiquity of TBBPA in aquatic environments, it is inevitable that these chemicals will enter the olfactory chambers of fish via water currents. Nevertheless, the olfactory toxicity of TBBPA to aquatic organisms and the underlying toxic mechanisms have yet to be elucidated. Therefore, we investigated the olfactory toxicity of TBBPA in the goldfish Carassius auratus , a model organism widely used in sensory biology. Results showed that exposure to TBBPA resulted in abnormal olfactory-mediated behaviors and diminished electro-olfactogram (EOG) responses, indicating reduced olfactory acuity. To uncover the underlying mechanisms of action, we examined the structural integrity of the olfactory epithelium (OE), expression levels of olfactory G protein-coupled receptors (GPCRs), enzymatic activities of ion transporters, and fluctuations in neurotransmitters. Additionally, comparative transcriptomic analysis was employed to investigate the molecular mechanisms further. Our study demonstrates for the first time that TBBPA at environmentally relevant levels can adversely affect the olfactory sensitivity of aquatic organisms by interfering with the transmission of aqueous stimuli to olfactory receptors, impeding the binding of odorants to their receptors, disrupting the olfactory signal transduction pathway, and ultimately affecting the generation of action potentials.
研究领域:
环境毒理学
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水生生物学
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感觉生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY
·
2018
·Vol. 27
·期 3
·313-324
·
IF 1.987
Dong Huike,Lu Guanghua,Yan Zhenhua,Liu Jianchao,Yang Haohan,Nkoom Matthew
As a cycloaliphatic brominated flame retardant, hexabromocyclododecane (HBCD) has been widely used in building thermal insulation and fireproof materials. However, there is little information on the bioconcentration as well as effects with respect to HBCD exposure in the aquatic environment. To investigate the bioconcentration of HBCD in tissues (muscle and liver) and its biochemical and behavioural effects, juvenile crucian carp ( Carassius auratus ) were exposed to different concentrations of technical HBCD (nominal concentrations, 2, 20, 200 μg/L) for 7 days, using a flow-through exposure system. HBCD was found to concentrate in the liver and muscle with a terminal concentration of 0.60 ± 0.22 μg/g lw (lipid weight) and 0.18 ± 0.02 μg/g lw, respectively, at an environmentally-relevant concentration (2 μg/L). The total thyroxine and total triiodothyronine in the fish plasma were lowered as a result of exposure to the HBCD. Acetylcholinesterase activity in the brain was increased, while swimming activity was inhibited and shoaling inclination was enhanced after exposure to 200 μg/L HBCD. Feeding rate was suppressed in the 20 and 200 μg/L treatment groups. In summary, HBCD concentrations 10–100× higher than the current environmentally-relevant exposures induced adverse effects in the fish species tested in this study. These results suggest that increasing environmental concentrations and/or species with higher sensitivity than carp might be adversely affected by HBCD.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 116
·期 1
·10
·
IF 2.2
Huang Junyun,Liu Boying,Yuan Sijia,Zhou Haiyun,Bao Jian,Tu Yong,Wang Zunyao,Wang Weixia
The UV filter benzophenone-3 (BP-3) is a prevalent environmental pollutant due to its extensive use in personal care products, raising concerns about its ecological risks. This study investigated the pH-dependent oxidative stress effects of benzophenone-3 (BP-3) on Carassius auratus by measuring four key biomarkers—superoxide dismutase, catalase, glutathione, and malonaldehyde in liver tissue. The results demonstrated that BP-3 exposure led to significant oxidative stress, as evidenced by altered antioxidant levels and increased lipid peroxidation. The integrated biomarker response indicated that stress was more pronounced at higher BP-3 concentrations and varied with pH, being greatest under acidic conditions. These findings demonstrate that the toxicity of BP-3 is strongly influenced by environmental pH, highlighting the necessity of incorporating pH variations into ecological risk assessments of ionizable organic pollutants.
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Qing Li,Xiaohui Xue,Shun Zou,Bin He
Phoxim, a typical organophosphorus pesticide widely used in agriculture and aquaculture, poses significant ecotoxicological risks to fish species due to its environmental residues. In this study, we focused on the liver, the core detoxification organ, systematically investigated the transcriptomic responses of Carassius auratus exposed to different concentrations (low, medium, high) of phoxim for 24 h and 96 h. Integrating WGCNA with qRT-PCR validation, we elucidated the dynamic, stage-specific toxic mechanisms and adaptive responses. The results demonstrated that phoxim toxicity exhibits marked time-dependent accumulation and concentration dependence. A total of 30,540 differentially expressed genes (DEGs) were enriched at 24 h (defense phase), increasing to 38,320 DEGs at 96 h (adaptation phase). With increasing concentration, the number of DEGs decreased at 24 h but increased at 96 h. GO and KEGG enrichment analyses revealed that 24 h exposure triggered broad cellular stress and defense responses, whereas 96 h exposure shifted toward concentration-dependent pathological processes: low concentrations continuously activated detoxification and tissue remodeling pathways, medium concentrations were dominated by sustained signaling pathway perturbations, and high concentrations exhibited energy depletion and amino acid metabolic disorders. WGCNA identified functionally distinct co-expression modules, constructing a dynamic model spanning homeostasis (Cyan module), defense (Blue, Green, and Purple modules), and adaptation (Yellow module). qRT-PCR validation of key CYP450 isoforms showed concentration- and time-dependent induction patterns, with sustained high expression even at 96 h under high concentration, corroborating the significant enrichment of the drug metabolism-cytochrome P450 pathway in the Yellow module. This study presents comprehensive analysis of time- and concentration-dependent phoxim effect on C. auratus liver cells gene transcription modulation.
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DOI
PubMed
高影响因子
Animals
·
2025
·Vol. 15
·期 5
·698
·
IF 2.7
Xi Shi,Jiayin Xu,Yujie Hou,Zhen Wei,Lufang Guo,Xiao Ma,Limin Wu,Wenge Ma,Xue Tian,Khor Waiho,Xuejun Li
Simple SummaryThe sperm characteristics are various among fish species, which leads to different methods of sperm preservation for specific species. The sperm preservation technique for Qihe crucian carp, one of the major economic aquacultural fish species in China, has not been reported previously. This study aimed to construct the method of sperm preservation for Qihe crucian carp. The results showed that Hank’s Balanced Salt Solution (HBSS) was the best extender solution, and 15% MeOH and 20% DMSO were the optimal cryopreservation solutions for low-temperature storage and liquid nitrogen preservation, respectively. Further, under current conditions, an ultra-freezer (−80 °C) could not replace liquid nitrogen for sperm cryopreservation in Qihe crucian carp. This is the first study to investigate the preservation of Qihe crucian carp sperm, which will provide valuable technical support for both genetic resource conservation and artificial breeding programs.Qihe crucian carpCarassius auratusis one of the major economic aquacultural fish species in China. Due to environmental degradation and the impact of the reproduction mode of gynogenesis, the wild population of Qihe crucian carp exhibits an extremely low proportion of males. Therefore, it is imperative to develop the sperm preservation technology for Qihe crucian carp. In this study, the indicators, including sperm motility, sperm movement time, and sperm lifetime, were applied to evaluate the preservation effects of various extenders, cryoprotectant types and concentrations, and preservation manners, thus establishing the techniques of sperm short-term storage and cryopreservation for Qihe crucian carp. The results showed that HBSS was the most suitable extender solution, in which the sperm motility reached 93.00%. Under room temperature (26 °C), the sperm motility showed no significant differences across six different cryopreservation solutions. Under low temperature (4 °C), a 15% MeOH solution presented the most effective preservation effects of sperm in the short-term low-temperature storage. For cryopreservation in liquid nitrogen, the sperm activity in 20% DMSO was significantly higher than that in other solutions, representing the optimal cryopreservation solution. Furthermore, it was found that the sperm activity preserved by liquid nitrogen was significantly higher than that preserved by the ultra-freezer (−80 °C), indicating that the ultra-freezer cannot substitute for liquid nitrogen in cryopreservation. This is the first study to investigate the preservation of Qihe crucian carp sperm, providing valuable technical support for both genetic resource conservation and artificial breeding programs.
研究领域:
水产养殖学
·
生殖生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Huike Dong,Guanghua Lu,Zhenhua Yan,Jianchao Liu,Yong Ji
Perfluorooctanoic acid (PFOA) has long been produced and widely used due to its excellent water and oil repellent properties. However, this trend has facilitated to the ubiquitous existence of PFOA in environmental matrix, and the potential ecotoxicity on aquatic organisms has not been fully elucidated. To study the tissue-specific bioconcentration and the nervous system- and energy-related biochemical effects of PFOA, as well as the phenotypic alterations by this chemical, male crucian carp ( Carassius auratus ) were exposed to gradient concentrations of PFOA (nominal 0.2, 10, 500 and 25,000 μg/L) in a flow-through apparatus for 7 days. PFOA was enriched in tissues following an order of blood > kidney ≥ liver > gill > brain > muscle. The bioconcentration factors ranged from 0.1 to 60.4. Acetylcholinesterase activity in the fish brain was inhibited, while liver carboxylesterase was induced in most cases and attenuated with time. The acyl-CoA oxidase activity was dose-dependently elevated and accompanied by a decline of ATP contents. PFOA treatments also inhibited the activity of the electron transport system (ETS). At the transcriptional level, ETS component complexes II and IV were concordantly depressed, and ATP synthesis was also downregulated. The mRNA level of peroxisome proliferator activated receptor α was increasingly upregulated, with related downstream genes upregulated in varying degrees. The phenotypes showed patterns of increased liver pathology and reduced swimming activity. In summary, PFOA leads to adverse effects in Carassius auratus related to multiple aspects, which may be associated with the nervous system, fundamental energy metabolism and other unpredictable factors. The results obtained in this study are expected to help clarify the PFOA toxic mechanisms on energy relevance.
研究领域:
环境科学
·
毒理学
·
鱼类生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）