Analytical Methods
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2024
·Vol. 16
·期 26
·4373-4380
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IF 2.7
Xiaoran Shi,Hui Zhao,Han Zhang,Qunfang Li,Fangming Lou
A feasible and practicable “off–on” type of fluorescence strategy for highly selective screening of L-selenium-methylselenocysteine (L-SeMC) in selenium-enriched Cardamine violifolia was developed using g-C3N4-MnO2 nanocomposites as fluorescent probes. The g-C3N4 nanosheets can emit blue fluorescence at 320 nm excitation wavelength with a fluorescence quantum yield of 28%. When MnO2 was deposited onto g-C3N4 nanosheets, the fluorescence of the g-C3N4 nanosheets was quenched due to fluorescence resonance energy transfer (FRET). After the addition of L-SeMC, MnO2 was reduced to Mn2+, which eliminated FRET and fluorescence was restored. Based on this, a quantitative method for the determination of L-SeMC was established. The fluorescence intensity of g-C3N4-MnO2 nanocomposites showed a good linear relationship with the concentration of L-SeMC in the range of 0–45 μg mL−1, the limit of detection (LOD, 3σ/K) was 8.25 ng mL−1 and the correlation coefficient was 0.9904. Common selenium compounds such as SeO2, Na2SeO3, SeMet and SeCys caused weak fluorescence intensity, which means that the developed method is highly selective to detect L-SeMC in a series of selenium compounds. Meanwhile, the technique was evaluated by spiking L-SeMC standards in C. violifolia extraction solutions and with 9 C. violifolia extraction specimens, receiving excellent accordance with results from the commercially available atomic fluorescence spectroscopy method.
研究领域:
植物学
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分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiawei Cheng,Pengfei Han,Jingyi Wang,Wangting Zhou,Zhenzhou Zhu,Jingren He,Muci Wu,Rui Zhang
Drying is essential for preserving Cardamine violifolia , a selenium-rich Brassicaceae vegetable, and for producing powders for functional foods. However, the effects of different drying methods on its aroma and flavor precursors remain unclear. This study compared the volatile and glucosinolate profiles of C. violifolia subjected to freeze-vacuum drying (FVD), microwave-vacuum drying (MVD), hot-air drying (HAD), and sun drying (SD). HS-SPME-GC–O–MS identified 58 volatile compounds, including 22 aroma-active compounds. Eleven of these were confirmed as key aroma compounds based on odor activity values and aroma recombination/omission experiments. Benzyl isothiocyanate contributed predominantly to the characteristic pungency. Other key odorants included trans-2-hexenal, benzaldehyde, benzeneacetaldehyde, nonanal, β-cyclocitral, 1-octen-3-ol, ketoisophorone, and β-ionone, which contributed to green, sweet, and mushroom-like notes. FVD best preserved the pungent–green flavor of fresh samples, owing to low-temperature dehydration retaining the highest levels of benzyl isothiocyanate and aldehydes. MVD partially retained pungency and generated 3-ethyl-2,5-dimethylpyrazine, which is responsible for roasted nuances. HAD and SD substantially reduced the pungent aromas and enhanced sweet and musty notes. This shift is likely due to extended heat exposure and oxidation, which produced higher concentrations of nonanal, β-cyclocitral, 1-octen-3-ol, 3,5-octadien-2-one, ketoisophorone, β-ionone, and dihydroactinidiolide. Glucosinolate analysis revealed glucotropaeolin (benzyl glucosinolate) as the predominant component, consistent with its major degradation products (benzyl isothiocyanate and benzyl nitrile) in headspace. HAD retained the highest total glucosinolate content due to rapid moisture removal and the stability of glucosinolates at the applied drying temperature. These findings provide useful references for the flavor quality of Brassicaceae vegetables after drying.
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Hongxin Xie,Xue Tian,Lina He,Jincheng Li,Liwei Cui,Xin Cong,Bochong Tang,Yi Zhang,Zhiying Guo,Aiyu Zhou,Dongliang Chen,Liming Wang,Jiating Zhao,Yong-Liang Yu,Bai Li,Yu-Feng Li
Cardamine violifolia is a Se hyperaccumulator found in Enshi, China. In this study, spatial metallomics was applied to visualize the distribution and speciation of Se in a single seed of C. violifolia. It was found that Se reached 1729.89 ± 28.14 mg/kg and the main Se species were SeCys and SeMet in bulk seeds. Further in situ study on a single seed found that the methylated Se species located mostly in the episperm. This is the first visualized evidence of the in situ distribution of methylated Se species in the seeds of C. violifolia. In all, spatial metallomics finds a preferable accumulation of methylated Se species in the seed coat, which deepens the understanding of the tolerance of Se by C. violifolia. The protocol applied in this study may also be used for the understanding of the tolerance of heavy metals/metalloids in other hyperaccumulators.
研究领域:
植物科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2025
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IF 6.2
Hongfa Wang,Yong Liang,Naiyu Xiao,Xin Cong,Shuyi Li,Xu Chen,Zhenzhou Zhu
In this study, porous biochar prepared by the pyrolysis of Cardamine violifolia residues impregnated with metal salts (ZnCl 2 , MgCl 2 or FeCl 3 ) was used as support to immobilize cellulase for the enzymatic saccharification of Cardamine violifolia residues. The results showed that a higher specific surface area along with abundant mesopores favored the immobilization of cellulase. As such, biochar prepared from the pyrolysis of 40 wt% FeCl₃-modified biomass at 800 ℃ (BC-800–0.4Fe) achieved the maximum cellulase loading capacity of 483.03 mg/g under optimized immobilization conditions. The adsorption isotherm and adsorption kinetic experiments showed that cellulase adsorption on BC-800–0.4Fe was driven by both physical and chemical adsorption, which was also confirmed by the structural change of cellulase and BC-800–0.4Fe after immobilization through characterization using FTIR and XPS. Compared to free cellulase, the immobilized cellulase demonstrated significantly enhanced pH, thermal, and storage stability. Furthermore, at an immobilized cellulase dosage of 20 FPU/g and temperature of 60°C, the enzymatic hydrolysis of pretreated Cardamine violifolia residues using BC-800–0.4Fe immobilized cellulase for 72 h produced a glucose yield of 90.54 %, and the glucose yield still remained at 46.40 % after 10-cycle reuse of the immobilized cellulase.
研究领域:
生物能源与生物燃料
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酶技术
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生物炼制
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环境生物技术
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材料科学
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DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 34
·22082-22090
·
IF 4.036
Shiyu Zhu,Xin Cong,Zheng Sun,Zhe Chen,Xu Chen,Zhenzhou Zhu,Shuyi Li,Shuiyuan Cheng
Enzymatic synthesis of selenium (Se)-enriched peptides is vital for their application in supplementing organic Se. However, the poor stability and reusability of the free enzyme impedes the reaction. In this work, a highly stable immobilized Alcalase was synthesized by immobilizing Alcalase on tannic acid (TA) and polyethyleneimine (PEI) modified Fe3O4 nanoparticles (NPs). The optimal immobilization conditions for immobilized Alcalase were found at a TA/PEI (v/v) ratio of 1 : 1, pH of 10, and temperature of 40 °C, and the results from scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier Transform Infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) characterization confirmed the successful immobilization of Alcalase. The results of an enzyme property test showed that immobilized Alcalase had higher thermal and pH stability than free Alcalase, and retained 61.0% of the initial enzyme activity after 10 repetitions. Furthermore, the organic Se content of Se-enriched peptide prepared through enzymatic hydrolysis of Cardamine violifolia (CV) protein with immobilized Alcalase was 2914 mg kg−1, and the molecular weight was mainly concentrated in 924.4 Da with complete amino acid components. Therefore, this study proposes the feasibility of immobilized enzymes for the production of Se-enriched peptides.
研究领域:
生物技术
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酶学
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纳米技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）