CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Kamran Ashraf,Zebo Liu,Qamar uz Zaman,Muhammad Arshad,Waqas Qamar Zaman,Ali Shan,Junxiong Yu,Touseef ur Rehman,Yingping Zhuang,Meijin Guo,Ali Mohsin
Elicitation is a potent strategy to enhance secondary metabolite yield, and nanoparticle-mediated elicitation offers a compelling alternative to traditional methods. Carthamus tinctorius ( C. tinctorius ) is rich in bioactive chlorogenic acids (CGAs) within its suspension cells. However, the mechanism by which doped nanoparticles (NPs) stimulate the CGAs biosynthesis in the cells remains unclear. Therefore, this study first elucidated the synthesis and characterization of selenium (Se)-doped CeO 2 @Fe 3 O 4 NPs and then explored their regulatory effect and mechanism for the CGAs biosynthesis in C. tinctorius cells. Material characterization via TEM, XPS, XRD, and EDX analysis confirmed the successful synthesis of selenium-dopped CeO 2 @Fe 3 O 4 nanocomposite. Fe 3 O 4 , CeO 2 , Fe 3 O 4 @CeO 2 , and selenium-dopped CeO 2 @Fe 3 O 4 NP treatments all promoted the biosynthesis of CGAs in the cells. Notably, the group treated with 15 mg L -1 selenium-dopped CeO 2 @Fe 3 O 4 NPs showed best effect (37.04 mg g −1 , 1.92 times that of control). Finally, metabolome and transcriptome profiling revealed the differential alterations in the content of 45 primary metabolites and differential expression of 83 CGA biosynthesis-related genes. This suggested that selenium-doped CeO 2 @Fe 3 O 4 NPs up-regulated the expression of CGA biosynthesis-related genes, and consequently enhanced CGAs accumulation in the cells. This study provides the first evidence that selenium-doped CeO 2 @Fe 3 O 4 acts as nano-elicitors for the biosynthesis of CGAs.
研究领域:
植物学
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纳米生物学
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代谢组学
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转录组学
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DOI
高影响因子
MOLECULES
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2023
·Vol. 28
·期 2
·596
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IF 4.6
Qiongqiong Wang,Shiqi Liu,Long Xu,Bin Du,Lijun Song
Polysaccharides are the main bioactive components in safflower. In this study, safflower polysaccharides (SPs) were extracted by ultrasonic assisted extraction, and four purified safflower polysaccharide fractions (named SSP1, SSP2, SSP3, and SSP4, respectively) were obtained. The physicochemical properties and in vitro physiological activities of the four fractions were investigated. The molecular weights (MW) of the SSPs were 38.03 kDa, 43.17 kDa, 54.49 kDa, and 76.92 kDa, respectively. Glucuronic acid, galactose acid, glucose, galactose, and arabinose were the main monosaccharides. The Fourier transform infrared spectroscopy (FT-IR) indicated that the polysaccharides hadα- andβ-glycosidic bonds. Nuclear magnetic resonance (NMR) analysis showed that SSP1 had 6 different types of glycosidic bonds, while SSP3 had 8 different types. SSP3 exhibited relatively higher ABTS+scavenging activity, Fe+3-reduction activity, and antiproliferative activity. The results will offer a theoretical framework for the use of SPs in the industry of functional foods and medications.Keywords:safflower;polysaccharides;structures characterization;physiological activities
研究领域:
植物化学
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功能食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2025
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IF 5.9
Xiqian Tan,Anqi Sun,Shuaibo Gao,Zixiao Shen,Lijun You,Fangchao Cui,Xuepeng Li,Jianrong Li
The uric acid (UA) degrading Weissella cibaria X1 was employed for the fermentation of safflower ( Carthamus tinctorius L.). Compared with naturally fermented safflower, X1 fermented safflower significantly enhancing the antioxidant capacity and xanthine oxidase (XO) inhibition rate, decreased UA levels by 81.30%, XO activity by 99.02%, and creatinine levels by 27.08% based on a hyperuricemia (HUA) zebrafish model. Additionally, it upregulated the genes inhibiting UA synthesis—hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1 gene) 1.91 fold, and those promoting UA excretion—urate oxidase (UOX) gene 5.40 fold, organic anion transporter 1 (OAT1) gene 4.80 fold, hepatocyte nuclear factor 4 alpha (HNF4A gene) 3.29 fold, and polyvalent PDZ domain 1 (PDZK1) gene 57.46 fold. Subsequently, non-targeted metabolomics analysis showed that X1 fermentation downregulated the content of L-glutamine and modulated purine metabolism than the safflower fermented naturally, thus decreasing the levels of purines and increasing those of scolymoside. Our research provided a potential HUA therapy via both pharmaceutical and nutraceutical strategies.
研究领域:
微生物学
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食品科学
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代谢组学
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药理学
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分子生物学
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营养学
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天然产物化学
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产品关联
DOI
高影响因子
Foods
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2025
·Vol. 14
·期 19
·3381
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IF 5.1
Jiaqi Liu,Hao Duan,Li Wang,Rui Qin,Jiao Liu,Hong Liu,Shuyuan Bao,Wenjie Yan
This study aimed to systematically characterize the volatile organic compound (VOC) profiles of safflower (Carthamus tinctoriusL.) from eight major production regions, providing a scientific basis for quality evaluation and geographical traceability. VOC profiling was conducted using gas chromatography–ion mobility spectrometry (GC–IMS), and regional differences were assessed through multivariate statistical analyses, including Principal Component Analysis (PCA), Orthogonal Partial Least Squares Discriminant Analysis (OPLS–DA), Euclidean distance, and hierarchical clustering. Key differential compounds were identified by variable importance in projection (VIP) and relative odor activity value (ROAV) analyses, with aldehydes and esters emerging as the primary contributors to the discrimination of samples across regions. VOC fingerprints of safflower were further established, and a combined VIP–ROAV strategy was proposed for the screening of characteristic compounds. These findings provide a reliable reference for safflower quality control and offer practical guidance for its geographical authentication in the food industry.
研究领域:
植物化学
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代谢组学
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食品科学
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分析化学
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天然产物化学
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质量控制
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地理标志产品
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
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2016
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IF 2.937
Jian-ping Li,Jian-ming Guo,Yong-qing Hua,Kevin Yue Zhu,Yu-ping Tang,Bu-chang Zhao,Li-fu Jia,Jing Zhao,Zhi-shu Tang,Jin-ao Duan
Background Danhong injection (DHI) is quite often used in combination with low-dose aspirin (ASA, 75–325 mg daily) in clinic, particularly for the treatment of cardiovascular diseases. Exploring their interaction profile is of great clinical importance. Purpose The current study aims to explore the interaction between DHI and low-dose ASA in rats. Methods Sixty four rats were randomly divided into eight groups. Stomach and other four vital organs were collected for histological evaluation. Organs which exhibited histological changes were selected for a further study to evaluate the damage score and mode of action. We tested the protective effect of DHI on gastric mucosal damage in different regimes of administration. COX activity, gastric mucus secretion , pepsin activity, antioxidant activity and ROS level were assayed to reflect the protective effect of DHI on gastric mucosal damage induced by ASA. Results Stomach was the target organ of interaction when DHI and ASA were used in combination. DHI alleviated gastric mucosal damage by 55.8% when DHI was injected before ASA (Group E) and by 53.5% when DHI was injected 2 h after ASA administration (Group F). Additionally, if DHI treatment was appended to the long-term administration of ASA, DHI still decreased the gastric mucosal damage score in 52.0% from 2.50 to 1.20. DHI improved gastric mucus secretion, as well as decreased pepsin activity to maintain the integrity of gastric mucosal barrier ( P < 0.05). Furthermore, DHI recovered antioxidant activity which was impaired by ASA. In details, DHI decreased gastric mucosal ROS level, increased CAT , GSH-Px and SOD activity, and reduced MDA concentration ( P < 0.05). When ASA (71.9 µM) was used in combination with DHI (23–fold dilution, presented in terms of concentrations of DSS , PA, SaD RA , SaB and SaA were 6.45–6.92, 1.10–1.14, 1.09–1.10, 0.86–0.90, 16.76–19.38 and 1.83–1.94 µg/ml, respectively) in vitro , the inhibition rate of ASA increased from 38.6% (ASA alone) to 62.8% (ASA-DHI) on COX-1 and from 28.9% (ASA alone) to 38.8% (ASA-DHI) on COX-2 ( P < 0.05). DHI strengthened the inhibition activity of ASA on both COX-1 and COX-2, which showed that DHI alleviated ASA induced gastric mucosal damage but not antagonized anti-COX effect of ASA. Conclusions Gastric protective benefits were clearly produced when DHI and ASA were used in combination, which provided rational guidance for clinical combined application of DHI and ASA.
研究领域:
药理学
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胃肠病学
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中医药学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
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IF 7.514
Xin Tong,Jiehong Yang,Yu Zhao,Haofang Wan,Yu He,Ling Zhang,Haitong Wan,Chang Li
Natural deep eutectic solvents (NaDESs) are promising green alternatives to conventional solvents widely applied in the extraction of natural products due to their physical and chemical superiorities. In present study, 22 NaDESs consisted from food grade ingredients were screened in ultrasonic assisted extraction (UAE) of bioactive compounds from safflower. The oral bioavailabilities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (ASYB) in the extracts were then investigated in SD rats with the help of HPLC-MS technique. The results revealed that l -proline-acetamide ( l -Pro-Am) was an effective solvent with the yields of HSYA and ASYB at 32.83 and 8.80 mg/g. Pharmacokinetic studies revealed that the blood level of HSYA and ASYB were significantly higher after oral administration of l -Pro-Am extract than that of aqueous extract. Especially, the relative bioavailabilities (to aqueous extract) of HSYA and ASYB were calculated 183.5% and 429.8%.
研究领域:
药理学
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化学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Kai-Yu Ruan,Teng-Fei Lu,Jing Zhou,Xin Liang,Sheng Wang,Yu-Qian Xia,Tong Wang
The utilization of bio-derived solvents holds immense promise in the extraction of natural products. In the present study, a systematic evaluation was conducted to assess the performance of bio-derived solvents in simultaneously extracting polysaccharides and phenolic substances from Carthamus tinctorius L. flower. Among them, ethyl lactate (24.7%) exhibited superior efficacy. A comprehensive investigation was also conducted on the salt response behavior of ethyl lactate and its in-situ enrichment efficiency for polysaccharides and phenolics, demonstrating that the recovery yield, enrichment factor, and separation factor can be finely tuned by manipulating the system composition. Based on these findings, we have developed a highly efficient ultrasound-assisted method that is responsive to salt and enables simultaneous extraction and in situ enrichment of polysaccharides and phenolics. In comparison with the method in Chinese Pharmacopoeia (2020), the extraction yield of Carthamus tinctorius L. flower quality markers is increased by more than 1.14 times, while the extraction time is shortened by over 4 times. Additionally, the amount of solvent used is reduced by a factor of 5.73, eliminating the need for methanol and corrosive HCl that are harmful to the environment. The findings of this study will provide crucial theoretical guidance for the application of bio-derived solvents, demonstrating significant potential in terms of quality control and large-scale preparation of natural medicines.
研究领域:
药学
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天然产物化学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Qingfa Tang,Yankui Yi,Yao Chen,Ziming Zhuang,Feng Wang,Lu Zhang,Shenkun Wei,Yusheng Zhang,Yueqiusha Wang,Li Liu,Qiang Liu,Cuiping Jiang
Salvia miltiorrhiza and Carthamus tinctorius are traditional Chinese medicines that have been widely used for the treatment of cardiovascular disease. Salvianic acid A (SAA), salvianic acid B (SAB), protocatechuic aldehyde (PCA) and hydroxysafflor yellow A (HSYA) are the major hydrophilic polyphenols of Salvia miltiorrhiza and Carthamus tinctorius , all of which have been documented as active compounds for the prevention and treatment of atherosclerosis (AS). However, high aqueous solubility, low permeability and poor stability properties of the four hydrophilic polyphenols might influence their bioavailability and thus hinder their clinical potential. In this work, we introduced a green and highly efficient method for the efficient delivery of the four hydrophilic components via metal-phenolic network. The four coordination polymers of SAA, SAB, PCA and HSYA were successfully fabricated, and confirmed by UV–vis, FTIR , XPS , ICP-MS and dynamic light scattering analysis. We found all of them displayed potent antioxidant activity , good biocompatibility and stability. Impressively, the four coordination polymers showed remarkably enhanced anti-atherosclerotic effect compared with free drugs. Collectively, metal-phenolic network-based coordination polymer might show great potential for safe and efficient delivery of the hydrophilic polyphenols of Salvia miltiorrhiza and Carthamus tinctorius for anti-atherosclerotic therapy.
研究领域:
药学
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纳米技术
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中药
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心血管疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）