Journal of Ocean University of China
·
2019
·Vol. 19
·期 2
·439-445
·
IF 0.699
Zhang Meijian,Ma Yurong,Che Xinyi,Huang Zumei,Chen Peng,Xia Guanghua,Zhao Meihui
This study investigated the difference of nutrient composition in Caulerpa lentillifera collected from different regions. The nutrient compositions of C. lentillifera from China’s Hainan and Shandong provinces were determined and compared with those from Semporna (Malaysia), Sabah (Malaysia), Petchburi (Thailand), and two other species of seaweeds (Malaysia). The results showed that the polysaccharide and protein contents of C. lentillifera from Hainan (44.82%±0.98% and 12.5% ±0.70%), Shandong (43.22% ±1.42% and 14.7% ±0.72%), Petchburi (59.27%±2.31% and 12.49%±0.30%), and Sabah (44.02%±2.01% and 19.38%± 1.48%) were higher than those of Eucheuma cottonii (26.49%±3.01% and 9.76%±1.33%) and Sargassum polycystum (33.49%± 1.70% and 5.40% ± 0.07%), respectively. The polyunsaturated fatty acid contents in C. lentillifera from Hainan (29.98%) and Shandong (22.11%) were higher than that in Semporna (16.76% ± 0.27%), Sabah (9.49%), and S. polycystum (20.34% ±0.43%), but lower than that in E. cottonii (51.55% ± 0.57%). In Caulerpa lentillifera of Hainan and Shandong provinces, the essential amino acid (EAA)/total amino acid (TAA) ratios were 35.22% and 38.78%, respectively; and the EAA/ETAA ratios were 54.36% and 63.35%, respectively. The EAA composition of C. lentillifera was close to the ideal model of Food and Agriculture Organization/World Health Organization. C. lentillifera was rich in mineral elements, including calcium ((3315.85 ±127.55) mg (100 g) −1 and (3728.35 ±92.38) mg (100 g) −1 ), magnesium ((6715.74 ± 82.58) mg (100 g) −1 and (8128.59 ± 242.72) mg (100 g) −1 ), and trace elements, including iron ((510.65 ±5.47) mg (100 g) −1 and (1972.97 ±183.35) mg (100 g) −1 ), selenium ((1.04 ±0.08) mg (100 g) −1 and (0.83 ±0.15) mg (100 g) −1 ), and zinc ((33.90 ±0.13) mg (100 g) −1 and (11.75 ±2.05) mg (100 g) −1 ). These results showed that C. lentillifera was more nutritious than S. polycystum or E. cottonii in terms of polysaccharide, protein, and fatty acid contents. Furthermore, both C. lentillifera species collected from Hainan and Shandong provinces show more basic nutrients. Therefore, C. lentillifera has important development and application prospects.
研究领域:
海洋生物学
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营养学
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食品科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ying You,Chen Song,Yinghuan Fu,Yujiao Sun,Chengrong Wen,Beiwei Zhu,Shuang Song
Caulerpa lentillifera polysaccharide (CLP) has been characterized as a sulfated polysaccharide which can effectively inhibit lipid digestion. However, little information was known regarding its inhibitory mechanisms. In the present study, desulfation and degradation were conducted to prepare the derivatives of CLP, and a series of chemical and spectroscopic methods were used to elucidate the structure-activity relationship of CLP on the inhibitory effect of lipid digestion. Results revealed that CLP possessed excellent binding capacities for sodium cholate, sodium glycocholate, and sodium taurocholate. In addition, CLP can effectively inhibit lipase activity by quenching the fluorescence intensity, changing the secondary structure , and decreasing the UV–Vis absorbance. Of note, sulfate groups in CLP took a vital role in inhibiting lipase activity, while the molecular weight of CLP showed a positive correlation with the binding activities of bile acids . Furthermore, adding CLP into the whey protein isolate (WPI) emulsion system also impeded lipid digestion, indicating that CLP can be a potential reduced-fat nutraceutical used in food emulsion systems.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2024
·Vol. 13
·期 10
·1268
·
IF 6
Weilong Zhou,Haolong Zheng,Yingyin Wu,Junyi Lin,Xiaofei Ma,Yixuan Xing,Huilong Ou,Hebert Ely Vasquez,Xing Zheng,Feng Yu,Zhifeng Gu
The escalating impact of human activities has led to the accumulation of microplastics (MPs) and heavy metals in marine environments, posing serious threats to marine ecosystems. As essential components of oceanic ecosystems, large seaweeds such as Caulerpa lentillifera play a crucial role in maintaining ecological balance. This study investigated the effects of MPs and cadmium (Cd) on the growth, physiology, biochemistry, and Cd accumulation in C. lentillifera while elucidating the underlying molecular regulatory mechanisms. The results demonstrated that exposure to MPs alone significantly promoted the growth. In contrast, exposure to Cd either alone or in combination with MPs significantly suppressed growth by reducing stem and stolon length, bud count, weight gain, and specific growth rates. Combined exposure to MPs and Cd exhibited the most pronounced inhibitory effect on growth. MPs had negligible impact while Cd exposure either alone or combined with MPs impaired antioxidant defenses and exacerbated oxidative damage; with combined exposure being the most detrimental. Analysis of Cd content revealed that MPs significantly increased Cd accumulation in algae intensifying its toxic effects. Gene expression analysis revealed that Cd exposure down-regulated key genes involved in photosynthesis, impairing both photosynthetic efficiency and energy conversion. The combined exposure of MPs and Cd further exacerbated these effects. In contrast, MPs alone activated the ribosome pathway, supporting ribosomal stability and protein synthesis. Additionally, both Cd exposure alone or in combination with MPs significantly reduced chlorophyll B and soluble sugar content, negatively impacting photosynthesis and nutrient accumulation. In summary, low concentrations of MPs promoted C. lentillifera growth, but the presence of Cd hindered it by disrupting photosynthesis and antioxidant mechanisms. Furthermore, the coexistence of MPs intensified the toxic effects of Cd. These findings enhance our understanding of how both MPs and Cd impact large seaweed ecosystems and provide crucial insights for assessing their ecological risks.
研究领域:
海洋生态学
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环境毒理学
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分子生物学
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植物学
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DOI
PubMed
高影响因子
AQUACULTURE
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2023
·
IF 4.5
Tingting Ye,Min Li,Yuanbin Lin,Zengjian Su
Aquaculture has expanded significantly in recent years, and the effluent it produces has had a significant negative impact on the environment. The survival and growth rates of aquaculture products may impact by the eutrophication of the water body if the effluent from aquaculture is not handled. In this study, chitosan-based aerogel was used as a carrier to immobilize the degrading bacteria, and the adsorption capacity of the carrier material for methyl blue was 1.30 mg·g −1 . The Caulerpa lentillifera was added to the shrimp tank at the same time as a biofilter , and a composite in-situ treatment system using microbes and macroalgae was built to handle the effluent from the mariculture industry to test the reduction rates of the nutrients from the wastewater. The outcomes demonstrated that the immobilized bacteria used had reduction rates of COD , NH 4 + and NO 2 − in wastewater that were 48.82%, 9.14% and 40.74% greater than those of the free bacteria, respectively. The reduction effect of macroalgae for nutrients in wastewater is also very ideal, in which the reduction rates of TP and TN reach 32.43% and 74.94% respectively. When the immobilized degrading bacteria and macroalgae are combined, the reduction rates of COD, TN and TP in water reach 68.61%, 59.03% and 34.25%. At the same time, the survival rate of shrimp and the biomass of macroalgae have increased to a positive extent, with increases of 12.5% and 140%, respectively. These results demonstrate that the introduction of immobilized degrading bacteria and macroalgae into the shrimp culture system can effectively reduce the accumulation of nutrients in the shrimp culture system and have a positive impact in a variety of ways.
研究领域:
环境科学
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水产养殖
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废水处理
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生物技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 3
·1474-1486
·
IF 3.3
Chuanli Zhang,Jiang Wang,Huiying Du,Xintong Han,Changjiu Li,Danni Huang,Xiaolong Yu
Summary The effects of Caulerpa lentillifera (CL) extract incorporation on antioxidant activity, antibacterial activity, water stability, and physical properties of the chitosan/gelatin (CS/GEL) films were studied. The CL extract containing CS/GEL composite film was prepared by the casting method. The effect of CL content (1%, 3%, 5%) on the antioxidant properties, thermal property, mechanical properties, barrier properties, water stability and water contact angle of CS/GEL films was evaluated. With the increase of CL extract content, bumps and micro-pores appeared on the surface and cross-section of the composite films. The water contact angle, water solubility, and water absorption swelling rate of the composite films were improved with the addition of CL; therefore, the composite films exhibited better water stability. The TS of films were enhanced (from 12.57 to 23.66 MPa). Compared with the normal CS/GEL films, the CS/GEL-CL films exhibited better antioxidant with DPPH scavenging capacity values of 72.6% and could effectively slow down the lipid oxidation of refrigerated pork samples during storage, such as lower accumulation of free fatty acid content and fluorescence compound formation. This suggested that CS/GEL-CL film might be suitable as active films for food packaging.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 8
·3840-3849
·
IF 4.125
Ying You,Haoran Song,Linlin Wang,Zhengqi Liu,Xiaoming Guo,Chunqing Ai,Shuang Song,Beiwei Zhu
BACKGROUND Caulerpa lentillifera has received extensive attention regarding expansion of its farming and increasing consumption. In our previous study, the structure of C. lentillifera polysaccharide (CLP) was elucidated. However, little information is available about its health effects. In this study, the anti-obesity effect of CLP was investigated by using a high-fat diet-induced obese mice model with two different supplementation methods. RESULTS In vitro simulated digestion results showed that CLP significantly decreased the lipid digestibility and induced the lipid droplets aggregation in the intestinal stage to inhibit the absorption of lipids. As revealed by 16S ribosomal RNA sequencing and non-targeted metabolomics, supplement of CLP by both pre-prandial gavage and free feeding patterns effectively prevented mice obesity via ameliorating intestinal flora disturbance and regulating bile acids circulation metabolism. Of note was that CLP administration had no effect on short-chain fatty acids production, suggesting the anti-obesity effect was uncorrelated with their production. Moreover, pre-prandial administration of CLP had a better anti-obesity effect in lowering body weight and serum lipid levels, but the free feeding resulted in a higher α -diversity of gut microbiota. CONCLUSION The findings of this study indicate that CLP could be a potential anti-obesity nutraceutical and that pre-prandial supplement of CLP may be a better intake method to exhibit its hypolipidemic effect. © 2022 Society of Chemical Industry.
研究领域:
营养学
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药理学
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微生物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Tingting Ye,Min Li,Yuanbin Lin,Bingtao Wei,Zengjian Su
With the vigorous development of the mariculture industry, the untreated wastewater from mariculture has exerted significant pressure on the water environment. The untreated N and P in the wastewater from mariculture can deteriorate the quality of the mariculture. In this study, a composite in situ treatment system involving macroalgae ( Caulerpa lentillifera / Caulerpa sertularoides f. Longipes ) and immobilized degrading bacteria was established to handle wastewater from shrimp culture. The changes in nutrients in aquaculture wastewater were studied by chemical analysis, and the microbial community structure was analyzed using molecular biology technology and high-throughput sequencing technology. The removal efficacy of nutrients in aquaculture wastewater and the composition of microorganisms in the wastewater were examined, and the primary causes for the alteration of the microbial community were analyzed. The results demonstrated that when the macroalgae in the system were Caulerpa lentillifera ( CL ), the removal efficiencies of TN, PO 4 3- -P, and COD from shrimp culture were 59.04%, 34.26%, and 68.61% respectively. When the macroalgae was Caulerpa sertularoides f. Longipes ( CSF ), the removal efficiencies of TN, PO 4 3- -P, and COD generated by experimental shrimp culture were 51.50%, 33.69%, and 50.88% respectively. The biomass (wet weight) of both macroalgae species also increased, facilitating the removal of nutrients from the wastewater. Additionally, both Proteobacteria and Bacteroidetes were the dominant bacteria in the three samples, and the addition of the composite in-situ treatment system had no impact on the dominant bacteria in the water. The results of FAPROTAX analysis indicated that compared with the untreated samples, the abundances of methyl-functional bacteria and amino acid-functional bacteria in the samples increased due to organic matter such as COD produced during shrimp culture and the addition of feed, suggesting that shrimp culture can influence the abundances of functional bacteria in the water. In conclusion, the combined in situ treatment system can effectively eliminate nutrients from aquaculture wastewater, and the combined effect of macroalgae and immobilized degrading bacteria plays a vital role in this process.
研究领域:
水产养殖
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环境科学
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微生物学
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水处理
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）