RSC Advances
·
2017
·Vol. 7
·期 72
·45792-45798
·
IF 3.108
Huili Ye,Hao Wang,Faqiong Zhao,Baizhao Zeng
A stable graphene (GR)/CdS:Mn photocatalyst was synthesized through a simple and straightforward one-pot hydrothermal method, and it exhibited enhanced photoelectrochemical (PEC) response to glutathione. The catalyst was characterized by X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and UV-vis diffuse reflectance spectroscopy. It showed efficient separation of photogenerated charge carriers and low photo-corrosion. The doped Mn2+ effectively improved the band structure of CdS. Meanwhile, the introduced GR greatly enhanced and facilitated visible light absorption as well as electron transport. The GR/CdS:Mn based PEC sensor displayed more sensitive photocurrent response to glutathione than the pure CdS, CdS:Mn and GR/CdS based sensors. Furthermore, the response of the sensor was rapid and stable. The details of the additions and specific effects of each component were discussed. Under the optimized conditions, it presented wide detection range of 0.01 μM to 100 μM, low detection limit, as well as good reproducibility. The sensor could be applied to the determination of glutathione in tomato and ketchup samples.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bing Wang,Yu-Xiang Dong,Yu-Ling Wang,Jun-Tao Cao,Shu-Hui Ma,Yan-Ming Liu
A novel and simple photoelectrochemical (PEC) biosensing method for microRNA-21 (miRNA-21) detection is reported based on energy transfer (ET) between CdS:Mn doped structure (CdS:Mn) and Au nanoparticles (AuNPs). In this protocol, TiO 2 -CdS:Mn hybrid structure was used as a sensing platform for hairpin DNA immobilization. In the absence of miRNA-21, the immobilized DNA was in the hairpin form. In this state, the photocurrent of the electrode was greatly depressed, due to the effective ET effect produced by short interparticle distance between CdS:Mn and AuNPs. In the presence of miRNA-21, the hairpin DNA hybridized with miRNA-21 and changed into a more rigid, rodlike double helix, which forced the AuNPs away from the electrode surface, leading to obvious recover of photocurrent because of the vanished damping effect. Integrating the fine PEC performance of TiO 2 -CdS:Mn hybrid structure with the significant ET effect between CdS:Mn and AuNPs, the sensitive detection of miRNA-21 was realized in a linear range of 1.0 fM to 10.0 pM with a low detection limit of 0.5 fM. This method might be aussichtsreich for the detection of miRNAs and other biomarkers.
研究领域:
光电化学
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生物传感器
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纳米技术
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分子生物学
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分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Na Bin,Weili Li,Xuehua Yin,Xiaohua Huang,Qingyun Cai
A novel electrochemiluminescence (ECL) aptasensor was proposed for ultrasensitive detection of cytochrome c (cyt c) using CdS:Mn quantum dot-modified TiO 2 nanowires (NWs) as electrode. The Mn-doped CdS was deposited on the TiO 2 NWs by successive ion layer adsorption and reaction (SILAR) as ECL emitter, on which thiol-modified aptamer of cyt c was attached via Cd–S bond. Due to the high photo-electrical transfer efficiency, the as-prepared aptasensor shows high selectivity and sensitivity towards cyt c with a detection limit of 9.5 fM and a linear range from 50 fM to 125 pM.
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 8
·2045-2054
·
IF 4.157
Deng Xunxun,Yang Xinlan,Guan Xingxing,Song Jie,Wu Shuo
A photoelectrochemical (PEC) immunosensing strategy based on the multiple quenching of polydopamine nanoparticles (PDA NPs) to Mn 2+ -doped CdS-modified TiO 2 nanoparticles (TiO 2 /CdS:Mn) was designed for the highly sensitive detection of carcinoembryonic antigen (CEA). The uniform PDA NPs possessed good dispersibility, good biocompatibility, and abundant functional groups for biomolecule assembly. They also had unique photophysical properties, with light absorption spanning the visible to infrared light range. When the immune-recognition brought the PDA NPs close to the TiO 2 /CdS:Mn interface, the PDA NPs competed with TiO 2 /CdS:Mn to absorb light, consumed photoelectrons generated in the TiO 2 /CdS:Mn, and hindered the access of electron donors to photoactive materials. The contribution from these aspects thus led to a significant decrease in photocurrent. Benefiting from the multiple quenching mechanism, the PEC immunosensor showed high sensitivity for CEA detection. Under optimal conditions, a low detection limit of 0.02 pg/mL and a wide linear relationship from 0.1 pg/mL to 100 ng/mL were obtained. The immunoassay showed good reproducibility and stability, and good selectivity and high accuracy in serum sample analysis. In this regard, PEC immunosensors may have great application potential for screening tumor markers and the prevention and monitoring of serious diseases.
研究领域:
生物医学工程
·
肿瘤标志物检测
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Gao-Chao Fan,Li Han,Hua Zhu,Jian-Rong Zhang,Jun-Jie Zhu
An ultrasensitive photoelectrochemical sandwich immunoassay was developed to detect matrix metalloproteinase-2 (MMP-2, antigen, Ag) based on CdS:Mn/CdTe cosensitized TiO2 nanotubes (TiO2-NTs) and signal amplification of SiO2@Ab2 conjugates. Specifically, the TiO2-NTs electrode was first deposited with CdS:Mn by successive ionic layer adsorption and reaction technique and then further coated with CdTe quantum dots (QDs) via the layer-by-layer method, forming TiO2-NTs/CdS:Mn/CdTe cosensitized structure, which was employed as a matrix to immobilize capture MMP-2 antibodies (Ab1); whereas, SiO2 nanoparticles were coated with signal MMP-2 antibodies (Ab2) to form SiO2@Ab2 conjugates, which were used as signal amplification elements via the specific antibody–antigen immunoreaction between Ag and Ab2. The ultrahigh sensitivity of this immunoassay derived from the two major reasons as below. First, the TiO2-NTs/CdS:Mn/CdTe cosensitized structure could adequately absorb the light energy, dramatically promote electron transfer, and effectively inhibit the electron–hole recombination, resulting in significantly enhanced photocurrent intensity of the sensing electrode. However, in the presence of target Ag, the immobilized SiO2@Ab2 conjugates could evidently increase the steric hindrance of the sensing electrode and effectively depress the electron transfer, leading to obviously decreased photocurrent intensity. Accordingly, the well-designed photoelectrochemical immunoassay exhibited a low detection limit of 3.6 fg/mL and a wide linear range from 10 fg/mL to 500 pg/mL for target Ag detection. Meanwhile, it also presented good reproducibility, specificity, and stability and might open a new promising platform for the detection of other important biomarkers.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
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