JOURNAL OF FLUORESCENCE
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2015
·Vol. 25
·期 4
·897-905
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IF 1.927
Li Li,Zhang Qiaolin,Ding Yaping,Lu Yaxiang,Cai Xiaoyong,Yu Lurong
Based on the quenching of the fluorescence intensity of thioglycolic acid (TGA)-capped core-shell CdTe/ZnS nanoparticles (NPs) by vanillin, a novel, simple and rapid method for the determination of vanillin was proposed. In aqueous medium, the functionalized core-shell CdTe/ZnS NPs were successfully synthesized with TGA as the capping ligand. TGA-capped core-shell CdTe/ZnS NPs were characterized by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Factors affecting the vanillin detection were investigated, and the optimum conditions were also determined. Under the optimum conditions, the relative fluorescence intensity of CdTe/ZnS NPs was linearly proportional to vanillin over a concentration range from 9.4 × 10 −7 to 5.2 × 10 −4 M with a correlation coefficient of 0.998 and a detection limit of 2.6 × 10 −7 M. The proposed method was also employed to detect trace vanillin in cookies with satisfactory results.
研究领域:
分析化学
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纳米技术
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食品科学
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DOI
高影响因子
Polymer Chemistry
·
2021
·Vol. 12
·期 26
·3775-3783
·
IF 5.582
Bingfeng Shi,Jianhua Lü,Ying Liu,Yang Xiao,Changli Lü
Nano-light materials have attracted great interest in materials research due to their excellent fluorescent properties. The hybrid assembly of various nano-optical materials combines their advantages, extending the scope of potential applications. Herein, we report a fluorescence organic–inorganic hybrid nanostructure (T-PNI@QDs) based on binding red-emitting CdTe/ZnS quantum dots (QDs) to blue-emitting aggregation-induced emission (AIE)-active polymeric micelles in aqueous media with dual-channel emission. The AIE-active polymeric micelles were obtained by the self-assembly of thiol-terminated tetraphenylethylene (TPE) functionalized poly(N-isopropylmethacylamide) (T-PNI-SH) in water. An interesting assembly behaviour of transformation from a worm-like to a core–shell structure was observed by controlling the ratio of QDs/polymers due to the coordinated interaction between the QDs and the thiol groups. The dual-emission of T-PNI@QDs has obvious temperature-sensitive behaviour on account of PNIPAM. The novel fluorescent hybrid nanostructure was used as a probe to selectively detect picric acid (PA) in pure water. We expect this work will open up a new perspective for the construction of organic–inorganic hybrids based on different nano-light materials.
研究领域:
材料科学
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纳米技术
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光学材料
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化学传感
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Qian Yang,Jinhua Li,Xiaoyan Wang,Hailong Peng,Hua Xiong,Lingxin Chen
Single-component dual-emission nanoparticles were synthesized by chelating the organic ligand 8-hydroxyquinoline (HQ) to the surface of CdTe/ZnS quantum dots, namely CdTe/ZnQ 2 , and were used to construct a novel mesoporous structured molecular imprinting ratiometric fluorescence sensor by facile one-pot sol-gel polymerization for the visual detection of Brilliant Blue. The CdTe/ZnQ 2 had bimodal fluorescence belonging to CdTe and ZnQ 2 segments, respectively; significantly, the emission wavelength of CdTe was optimized to be 630 nm for the largest overlap with the absorption spectrum of Brilliant Blue. Consequently, fluorescence resonance energy transfer (FRET) efficiency was greatly enhanced, resulting in ideal determination. A favorable linearity toward Brilliant Blue was obtained within 0–1.0 μmol·L −1 along with profuse color evolution from orange to yellowish orange to yellowish green to green, and a high detectability of 8.8 nmol·L −1 was offered. Excellent recognition selectivity for Brilliant Blue over possibly coexistent food colorants was demonstrated, with a high imprinting factor of 7.1. Furthermore, endogenous Brilliant Blue was detected ranging from 0.21 to 41.03 mg/kg in six typical food samples with relative standard deviations lower than 3.5%, and the results agreed well with that afforded by conventional methods. Using Brilliant Blue as a model, this dual-emission color-controllable nanoparticle based imprinting ratiometric fluorescence sensor provided promising perspectives for the highly selective and sensitive, rapid, visual detection of colored substances in complicated matrices.
研究领域:
纳米技术
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分析化学
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材料科学
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食品科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2019
·
IF 6.35
Qian Yang,Jinhua Li,Xiaoyan Wang,Hua Xiong,Lingxin Chen
A novel ternary-emission fluorescence sensor was proposed by post-imprinting mixing blue-/green-/red-emission bovine hemoglobin (BHb) imprinted polymers (b-MIPs, g-MIPs, and r-MIPs) at a proper ratio and realized the multiplexed and visual detection of BHb. The three MIPs were individually embedded with blue-emission 7-hydroxycoumarin, green-emission CdTe quantum dots (QDs), and red-emission CdTe/ZnS QDs. Upon interaction with BHb within 8 min, the fluorescence of CdTe and CdTe/ZnS QDs were simultaneously turned off, whereas the 7-hydroxycoumarin turned on the fluorescence intensity. Thereupon, the ratiometric fluorescence intensity of the ternary emission linearly varied within 0.025–3 μM BHb, accompanying the profuse fluorescence color evolution from yellowish green to yellow to salmon to plum to purple to finally blue. In comparison with the dual- or single-emission sensor, the ternary-emission fluorescence MIPs sensor provided a wider color variation covering the green–red–blue window for accurate naked-eye determination of BHb, as well as a lower detection limit down to 7.8 nM and a higher imprinting factor of 15.2. Moreover, the satisfactory recoveries of 99.25–111.7% in determining the spiked BHb in bovine urine samples, as well as the optical stability and post-imprinting construction convenience, indicated that the developed tricolor-emission fluorescence MIPs sensor provided an ideal alternative for rapid, sensitive, and visual determination of proteins in complicated samples.
研究领域:
分析化学
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生物技术
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
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2014
·Vol. 6
·期 7
·2331-2337
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IF 1.938
Ying Yu,Ruo-Ying Li,Shuang-Shuang Wu,Bi-Xia Lin,Yu-Juan Cao,Xiao-Gang Hu,Jian-Zhong Wu
Plant hormones influence plant growth and development progress, and response to the environment. Therefore, it is highly significant to know the reasons that cause the above phenomena. Research on the signal transduction mechanism of hormones in plants is insufficient due to the lack of an accurate and sensitive method to measure it. The detection of binding sites between hormones and receptors is the first step in studying the signal transduction of plant hormones. The auxin indole-propionic acid (IPA) is an important plant hormone and can promote rooting and fruiting in plants. By conjugating CdTe/ZnS QDs with the IPA moiety, we constructed the CdTe/ZnS–IPA fluorescence probes for the in situ detection of IPA binding sites in plant tissues. By improving the synthesis conditions, we capped ZnS outside the oil bath-obtained CdTe to prepare red-emitting CdTe/ZnS QDs, and the core/shell structure of the CdTe/ZnS QDs was proved by XRD. The success of conjugation was verified by fluorescence spectra, fluorescence decay lifetime, UV-vis spectra, and DLS. The CdTe/ZnS–IPA with a 5.4% coupling rate were selected for the labelling of IPA binding sites, which are concentrated in the endodermis cells of mung bean root tissues. The existence of the competitive relationship of recognition for IPA binding sites between CdTe/ZnS–IPA and IPA declared that CdTe/ZnS–IPA possessed biological activity for IPA. The red-emitting CdTe/ZnS–IPA can contrast with the background of plant tissues compared to green-emitting CdTe–IPA. In sum, the novel probe showed low toxicity, was plant tissue background-resistant, and this new method for the selective detection of IPA binding sites in plant tissues has the advantages of convenience and easy visualization.
研究领域:
植物生物学
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分子生物学
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生物技术
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 21
·5437-5444
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IF 3.286
Jiao Xiangyu,Zhou Yabin,Zhao Dan,Pang Duo,Wang Changtao,Du Hongwu,Wen Yongqiang,Zhang Xueji
To meet the need for high-throughput immunoassays, many multiplex fluorescent immunoassays have been proposed. Most of them need different kinds of fluorescent label indicators during the test. In this work, a novel indirect ELISA-inspired dual-channel fluorescent immunoassay based on 3-mercaptopropionic acid capped CdTe/ZnS quantum dots (QDs) was constructed. The ELISA wells were coated with two kinds of antigen–QD complex. When the primary antibodies were present in a sample, they mediated the binding of a secondary antibody–DNA–gold nanoparticle complex to the antigen–QD complex. Then the gold nanoparticles quenched the fluorescence of the QDs and a decrease in fluorescence intensity was observed. Thus, the amount of primary antibody could be estimated from the decrease of fluorescence intensity. Owing to the wide absorption range and the relatively narrow emission band of the QDs, the dual-channel fluorescent immunoassay system could work at the same excitation wavelength and the emission wavelengths of each channel had no interference. As a result, two different kinds of primary antibody could be detected at the same time in one ELISA well, which simplified the operation and greatly improved the efficiency. Besides, only one type of secondary antibody needs to be added to the prepared microtiter plates, which further simplified the operation during the detection procedure. This dual-channel fluorescent immunoassay system will provide new insights into high-throughput immunodetection.
Graphical abstract
研究领域:
生物化学
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分析化学
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纳米技术
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免疫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）