JOURNAL OF LUMINESCENCE
·
2012
·
IF 2.102
Zhengqing Liu,Pengfei Yin,Huiping Gong,Pingping Li,Xiaodan Wang,Youqiu He
Aqueous glutathione (GSH)-capped CdTe / ZnS QDs with the diameter of 3–4 nm were synthesized. The fluorescence of CdTe / ZnS QDs at 577 nm was quenched in the presence of rifampicin (Rfp), with excitation wavelength at 350 nm. The mechanism of the interaction of CdTe / ZnS QDs with Rfp was investigated. Under the optimal conditions, the calibration plot of ln( F 0 /F ) was linear in the range 0.83–56 μg mL −1 with concentration of Rfp, and the detection limit was 0.25 μg mL −1 . The proposed method was successfully applied to the determination of Rfp in its commercial capsules, and satisfactory results were obtained. The recovery of the method was in the range 98.6–103.2%.
研究领域:
分析化学
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药学
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2015
·Vol. 25
·期 4
·897-905
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IF 1.927
Li Li,Zhang Qiaolin,Ding Yaping,Lu Yaxiang,Cai Xiaoyong,Yu Lurong
Based on the quenching of the fluorescence intensity of thioglycolic acid (TGA)-capped core-shell CdTe/ZnS nanoparticles (NPs) by vanillin, a novel, simple and rapid method for the determination of vanillin was proposed. In aqueous medium, the functionalized core-shell CdTe/ZnS NPs were successfully synthesized with TGA as the capping ligand. TGA-capped core-shell CdTe/ZnS NPs were characterized by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Factors affecting the vanillin detection were investigated, and the optimum conditions were also determined. Under the optimum conditions, the relative fluorescence intensity of CdTe/ZnS NPs was linearly proportional to vanillin over a concentration range from 9.4 × 10 −7 to 5.2 × 10 −4 M with a correlation coefficient of 0.998 and a detection limit of 2.6 × 10 −7 M. The proposed method was also employed to detect trace vanillin in cookies with satisfactory results.
研究领域:
分析化学
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纳米技术
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食品科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huiling Li,Li Tian,Shuning Yang,Chao Li,Yujia Song,Ruidan Li,Yanjia Guo,Juan Lu
A signal-on solid-state electrochemiluminescence (ECL) sensor based on luminol@carbon nanotubes (luminol@CNTs) and CdTe-ZnS@hydroxyapatite (CdTe-ZnS@HAP) was constructed for the detection of paclitaxel (PTX). In this sensing platform, luminol was used as the main luminophore and CdTe-ZnS was used as a co-reaction accelerator. CNTs and HAP were acted as carriers to adsorb more luminophores and co-reaction promoters. In addition, due to the enhanced effect of PTX on the ECL intensity of the luminol-O 2 system, PTX was introduced as the final detection target. Under the optimal experimental conditions, the ECL intensity change values (ΔI) of the sensor showed a good linear relationship with the logarithm of PTX concentration in the range of 3 × 10 -12 − 3 × 10 -7 molL -1 , and the limit of detection was 1 × 10 -12 mol L -1 (S/N = 3). This method was successfully applied to the determination of trace PTX concentration in serum.
研究领域:
分析化学
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药物检测
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纳米材料科学
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2021
·Vol. 12
·期 26
·3775-3783
·
IF 5.582
Bingfeng Shi,Jianhua Lü,Ying Liu,Yang Xiao,Changli Lü
Nano-light materials have attracted great interest in materials research due to their excellent fluorescent properties. The hybrid assembly of various nano-optical materials combines their advantages, extending the scope of potential applications. Herein, we report a fluorescence organic–inorganic hybrid nanostructure (T-PNI@QDs) based on binding red-emitting CdTe/ZnS quantum dots (QDs) to blue-emitting aggregation-induced emission (AIE)-active polymeric micelles in aqueous media with dual-channel emission. The AIE-active polymeric micelles were obtained by the self-assembly of thiol-terminated tetraphenylethylene (TPE) functionalized poly(N-isopropylmethacylamide) (T-PNI-SH) in water. An interesting assembly behaviour of transformation from a worm-like to a core–shell structure was observed by controlling the ratio of QDs/polymers due to the coordinated interaction between the QDs and the thiol groups. The dual-emission of T-PNI@QDs has obvious temperature-sensitive behaviour on account of PNIPAM. The novel fluorescent hybrid nanostructure was used as a probe to selectively detect picric acid (PA) in pure water. We expect this work will open up a new perspective for the construction of organic–inorganic hybrids based on different nano-light materials.
研究领域:
材料科学
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纳米技术
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光学材料
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化学传感
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Hai Chi,Guoqin Liu
Aflatoxin B1 (AFB1) is a toxic metabolite produced by fungi with substantial environmental and health concerns. Thus, the detection of AFB1 has attracted increasing attention. In this study, a fluorometric sandwich biosensor was developed for the detection of AFB1, which was prepared by combining molecularly imprinted polymer (MIP) with an aptamer. Porous carbon (PC) generated by the carbonization of ZIF-8 provided abundant sites for the MIP layer to achieve high-performance recognition of AFB1. The aptamer-modified CdTe/ZnS achieved efficient sandwich analysis of AFB1 as a signal probe. The sandwich biosensor exhibited excellent selectivity and high sensitivity with a dynamic linear range of 0.01–20 ng/mL and good reliability in spiked edible oil samples as low as 4.0 pg/mL. In sum, the developed sandwich biosensor is promising for the detection of AFB1 in food and the environment, with great potential in the control of other challenging food hazards .
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 21
·5437-5444
·
IF 3.286
Jiao Xiangyu,Zhou Yabin,Zhao Dan,Pang Duo,Wang Changtao,Du Hongwu,Wen Yongqiang,Zhang Xueji
To meet the need for high-throughput immunoassays, many multiplex fluorescent immunoassays have been proposed. Most of them need different kinds of fluorescent label indicators during the test. In this work, a novel indirect ELISA-inspired dual-channel fluorescent immunoassay based on 3-mercaptopropionic acid capped CdTe/ZnS quantum dots (QDs) was constructed. The ELISA wells were coated with two kinds of antigen–QD complex. When the primary antibodies were present in a sample, they mediated the binding of a secondary antibody–DNA–gold nanoparticle complex to the antigen–QD complex. Then the gold nanoparticles quenched the fluorescence of the QDs and a decrease in fluorescence intensity was observed. Thus, the amount of primary antibody could be estimated from the decrease of fluorescence intensity. Owing to the wide absorption range and the relatively narrow emission band of the QDs, the dual-channel fluorescent immunoassay system could work at the same excitation wavelength and the emission wavelengths of each channel had no interference. As a result, two different kinds of primary antibody could be detected at the same time in one ELISA well, which simplified the operation and greatly improved the efficiency. Besides, only one type of secondary antibody needs to be added to the prepared microtiter plates, which further simplified the operation during the detection procedure. This dual-channel fluorescent immunoassay system will provide new insights into high-throughput immunodetection.
Graphical abstract
研究领域:
生物化学
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分析化学
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纳米技术
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免疫学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2015
·Vol. 30
·期 7
·1064-1070
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IF 1.518
Wei Bian,Fang Wang,Hao Zhang,Lin Zhang,Li Wang,Shaomin Shuang
Core-shell CdTe/ZnS quantum dots capped with 3-mercaptopropionic acid (MPA) were successfully synthesized in aqueous medium by hydrothermal synthesis. These quantum dots have advantages compared to traditional quantum dots with limited biological applications, high toxicity and tendency to aggregate. The concentration of Cu 2+ has a significant impact on the fluorescence intensity of quantum dots (QDs), therefore, a rapid sensitive and selective fluorescence probe has been proposed for the detection of Cu 2+ in aqueous solution. Under optimal conditions, the fluorescence intensity of CdTe/ZnS QDs was linearly proportional to the concentration of Cu 2+ in the range from 2.5 × 10 –9 M to 17.5 × 10 –7 M with the limit of 1.5 × 10 –9 M and relative standard deviation of 0.23%. The quenching mechanism is static quenching with recoveries of 97.30–102.75%. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
分析化学
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纳米材料
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环境监测
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生物传感器
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DOI
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
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2019
·Vol. 40
·期 3
·388-402
·
IF 3.065
Tianshu Wu,Keyu He,Xue Liang,Tingting Wei,Yan Wang,Lingyue Zou,Ting Zhang,Yuying Xue,Meng Tang
The excellent optical property and relatively low toxicity of CdTe/ZnS core/shell quantum dots (QDs) make them an advanced fluorescent probe in the application of biomedicines, particularly in neuroscience. Thus, it is important to evaluate the biosafety of CdTe/ZnS QDs on the central nervous system (CNS). Our previous studies have suggested that the high possibility of CdTe/ZnS QDs being transported into the brain across the blood-brain barrier resulted in microglial activation and a shift of glycometabolism, but their underlying mechanism remains unclear. In this study, when mice were injected intravenously with CdTe/ZnS QDs through tail veins, the microglial activation, polarized into both M1 phenotype and M2 phenotype, and the neuronal impairment were observed in the hippocampus. Meanwhile, the increased pro- and anti-inflammatory cytokines released from BV2 microglial cells treated with CdTe/ZnS QDs also indicated that QD exposure was capable of inducing microglial activation in vitro. We further demonstrated that the glycolytic shift from oxidative phosphorylation switching into aerobic glycolysis was required in the microglial activation into M1 phenotype induced by CdTe/ZnS QD treatment, which was mediated through the mTOR signaling pathway. The findings, taken together, provide a mechanistic insight regarding the CdTe/ZnS QDs inducing microglial activation and the role of the glycolytic shift in it.
研究领域:
神经科学
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毒理学
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细胞代谢
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生物医学工程
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2014
·Vol. 29
·期 8
·1177-1182
·
IF 1.675
Lili Pang,Jian Xu,Chang Shu,Jin Guo,Xiaona Ma,Yu Liu,Wenying Zhong
High luminescence quantum yield water-soluble CdTe/ZnS core/shell quantum dots (QDs) stabilized with thioglycolic acid were synthesized. QDs were chemically coupled to fully humanized antivascular endothelial growth factor 165 monoclonal antibodies to produce fluorescent probes. These probes can be used to assay the biological affinity of the antibody. The properties of QDs conjugated to an antibody were characterized by ultraviolet and visible spectrophotometry, fluorescent spectrophotometry, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transmission electron microscopy and fluorescence microscopy. Cell-targeted imaging was performed in human breast cancer cell lines. The cytotoxicity of bare QDs and fluorescent probes was evaluated in the MCF-7 cells with an MTT viability assay. The results proved that CdTe/ZnS QD–monoclonal antibody nanoprobes had been successfully prepared with excellent spectral properties in target detections. Surface modification by ZnS shell could mitigate the cytotoxicity of cadmium-based QDs. The therapeutic effects of antivascular endothelial growth factor antibodies towards cultured human cancer cells were confirmed by MTT assay. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
纳米技术
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癌症研究
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分子成像
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生物偶联
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生物医学工程
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2014
·Vol. 20
·期 8
·2132-2137
·
IF 5.696
Bingxin Liu,Cuiyan Tong,Lijuan Feng,Chunyu Wang,Yao He,Changli Lü
Graphical Smooth revelation : A new type of water-soluble CdTe/ZnS quantum dot has been employed to construct a ratiometric fluorescent nanosensor for the detection of nitroexplosives in aqueous media (see scheme). The observed ratiometric fluorescence intensity change allows the quantitative detection of picric acid with a limit of 9 n M . (PA=picric acid; ET=electron transfer.) A ratiometric fluorescent probe based on dual luminescence QD/CPL for selective sensing of the nitroaromatic explosive picric acid (PA) was constructed. The observed ratiometric fluorescence intensity change allows the quantitative detection of PA with a detection limit of 9 n M .
研究领域:
分析化学
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材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Qian Yang,Jinhua Li,Xiaoyan Wang,Hailong Peng,Hua Xiong,Lingxin Chen
Single-component dual-emission nanoparticles were synthesized by chelating the organic ligand 8-hydroxyquinoline (HQ) to the surface of CdTe/ZnS quantum dots, namely CdTe/ZnQ 2 , and were used to construct a novel mesoporous structured molecular imprinting ratiometric fluorescence sensor by facile one-pot sol-gel polymerization for the visual detection of Brilliant Blue. The CdTe/ZnQ 2 had bimodal fluorescence belonging to CdTe and ZnQ 2 segments, respectively; significantly, the emission wavelength of CdTe was optimized to be 630 nm for the largest overlap with the absorption spectrum of Brilliant Blue. Consequently, fluorescence resonance energy transfer (FRET) efficiency was greatly enhanced, resulting in ideal determination. A favorable linearity toward Brilliant Blue was obtained within 0–1.0 μmol·L −1 along with profuse color evolution from orange to yellowish orange to yellowish green to green, and a high detectability of 8.8 nmol·L −1 was offered. Excellent recognition selectivity for Brilliant Blue over possibly coexistent food colorants was demonstrated, with a high imprinting factor of 7.1. Furthermore, endogenous Brilliant Blue was detected ranging from 0.21 to 41.03 mg/kg in six typical food samples with relative standard deviations lower than 3.5%, and the results agreed well with that afforded by conventional methods. Using Brilliant Blue as a model, this dual-emission color-controllable nanoparticle based imprinting ratiometric fluorescence sensor provided promising perspectives for the highly selective and sensitive, rapid, visual detection of colored substances in complicated matrices.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
食品科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Qian Yang,Jinhua Li,Xiaoyan Wang,Hua Xiong,Lingxin Chen
A novel ternary-emission fluorescence sensor was proposed by post-imprinting mixing blue-/green-/red-emission bovine hemoglobin (BHb) imprinted polymers (b-MIPs, g-MIPs, and r-MIPs) at a proper ratio and realized the multiplexed and visual detection of BHb. The three MIPs were individually embedded with blue-emission 7-hydroxycoumarin, green-emission CdTe quantum dots (QDs), and red-emission CdTe/ZnS QDs. Upon interaction with BHb within 8 min, the fluorescence of CdTe and CdTe/ZnS QDs were simultaneously turned off, whereas the 7-hydroxycoumarin turned on the fluorescence intensity. Thereupon, the ratiometric fluorescence intensity of the ternary emission linearly varied within 0.025–3 μM BHb, accompanying the profuse fluorescence color evolution from yellowish green to yellow to salmon to plum to purple to finally blue. In comparison with the dual- or single-emission sensor, the ternary-emission fluorescence MIPs sensor provided a wider color variation covering the green–red–blue window for accurate naked-eye determination of BHb, as well as a lower detection limit down to 7.8 nM and a higher imprinting factor of 15.2. Moreover, the satisfactory recoveries of 99.25–111.7% in determining the spiked BHb in bovine urine samples, as well as the optical stability and post-imprinting construction convenience, indicated that the developed tricolor-emission fluorescence MIPs sensor provided an ideal alternative for rapid, sensitive, and visual determination of proteins in complicated samples.
研究领域:
分析化学
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生物技术
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）