EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2022
·Vol. 2022
·期 20
·e202200202
·
IF 2.551
Zicong Tan,Ying Wang,Jie Zhang,Zhang Zhang,Samantha Sze Man Wong,Shiqing Zhang,Hongyan Sun,Ken Kin Lam Yung,Yung-Kang Peng
Graphical CeO 2 nanosphere enclosed by (100) and (111) surfaces has been reported to mimic phosphatase and peroxidase by catalyzing substrate dephosphorylation and oxidation (with H 2 O 2 ). We herein successfully boost the reaction specificity for this material by shape control. The acidic Ce species on octahedron (111) surface was found to selectively catalyze substrate dephosphorylation while its electron rich counterpart on cube (100) surface only promotes substrate oxidation. Among reported nanozymes, CeO 2 seems to be the only transition metal oxide that can mimic phosphatase and peroxidase by catalyzing substrate dephosphorylation and oxidation (with H 2 O 2 ). However, no consensus on the key Ce species was reached in the literature using spherical CeO 2 enclosed by (111) and (100) surfaces, not to mention the further control of its reaction specificity. In this study, octahedral and cubic CeO 2 preferentially terminated by (111) and (100) surfaces were found to exhibit high reaction specificity (and activity) towards each of the above reactions. Spectroscopic evidence suggests that this is closely associated with the Lewis acidity (or electron density) of surface Ce species. The acidic Ce species on (111) surface can catalyze substrate dephosphorylation at room temperature but do not for substrate oxidation with H 2 O 2 . This correlation was further evidenced by the electron-rich Ce species on (100) surface, hindering the first reaction while promoting the latter.
研究领域:
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiao-Jie Shi,Xin-Yi Dai,Yu Wu,Ting-Ting Zhang,Tian-Shun Song,Xiang-Ling Li,Jing-Jing Xie
Achieving a simple process, detailed information, and visual response for cancer diagnosis and treatment is desirable but challenging. Herein, we develop a self-cascade system-triggered colorimetric array for the sensitive detection of choline, a promising biomarker in triple-negative breast cancer (TNBC). The system utilizes a ChOx-Ce 3+ probe, where ChOx catalyzes the oxidation of target choline to betaine, generating H 2 O 2 , which then in situ reacts with Ce 3+ to form a CeO 2 nanozyme. This nanozyme exhibits robust oxidase (OXD)-like activity, oxidizing tetramethylbenzidine (TMB) to oxTMB, resulting in a visible color change that correlates with choline concentration. The self-triggered cascade mechanism significantly simplifies the detection process by eliminating the need for expensive materials and complex equipment. Additionally, it enhances the selectivity, resistance to protein interference, and long-term stability of the probe. The system demonstrated high accuracy in measuring choline concentrations in MDA-MB-231 cells and real samples, with recovery rates ranging from 97.4 % to 101 %. These results illustrate the potential of the self-cascade system-triggered colorimetric array for early TNBC detection and therapeutic monitoring, offering a cost-effective, user-friendly diagnostic tool for clinical applications.
研究领域:
生物化学
·
癌症诊断
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Lulu Cui,Houshen Li,Weijie Shi,Yingying Jing,Shuhong Sun,Shiyun Ai,Zeyi Guo
Reducing the size of active species is a powerful means to improve the utilization rate of active metals and enhance the properties of bimetallic nanozymes. In this work, Fe was introduced into Ce-MOF through the coordination of Fe 3+ and organic ligands, and the coordination effect resulted in atomically dispersed Fe in the derived Fe/CeO 2 nanozyme. Due to the atomically dispersed Fe embedded in the lattice of CeO 2 , a large number of defect sites were generated, endowing the nanozyme with excellent peroxidase (POD)-like activity. The constructed total antioxidant capacity (TAC) sensor based on the POD-like activity of Fe 1.01 /CeO 2 nanozyme displayed a very wide linear concentration range for AA, Cys, Glu and Trolox. More importantly, this TAC sensor can be applied to the TAC detection in vitamin C tablets and beverages. This work provides theoretical guidance for the synthesis of high-performance bimetallic nanozymes for TAC detection.
研究领域:
材料科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Cijun Shuai,Kangdong Wang,Shuping Peng,Jun Zan,Jiang Xiao,Shun Hu,Qi Zhong
CeO 2 nanozymes have garnered significant attention in chemodynamic therapy due to their peroxidase-like activity and ability to deplete glutathione. However, their catalytic efficiency is constrained by the low conversion rate of Ce 3+ /Ce 4+ . To overcome this limitation, the electron transfer was accelerated by introducing the transition metal Mn atom as a valence electron donor through lattice charge transfer. In detail, we introduced Ce 1-x Mn x O 2 into the poly-L-lactic acid (PLLA) scaffold fabricated by selective laser sintering. The conversion from Ce 3+ to Ce 4+ promoted the decomposition of H 2 O 2 within the tumor microenvironment to generate hydroxyl radicals with high oxidative activity, consequently inducing oxidative stress. The conversion from Ce 4+ to Ce 3+ depleted intracellular antioxidant glutathione, disrupting redox balance in tumor cells. This continuous redox cycle ultimately triggered apoptosis in tumor cells. Using a first-principles Hubbard-corrected approximate density-functional method, the analysis of the electron band structure revealed the presence of donor energy levels within the bandgap of Ce 1-x Mn x O 2 , enabling electron transfer channels around 0.645 eV. Electrochemical experiments confirmed that Ce 0.8 Mn 0.2 O 2 significantly reduced the activation overpotential from 0.907 V to 0.646 V, enhancing its redox capacity. As a result, the scaffold exhibited a threefold increase in tumor-killing rate. These findings highlight the immense potential of CeO 2 nanozymes in enhancing chemodynamic therapy for effective tumor treatment.
研究领域:
生物医学工程
·
肿瘤治疗学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Huan Jiang,Baihui Wang,Ruyi Tang,Yaoyu Tan,Mengyao Qi,Xia Zhang
Although thiourea and its derivatives are applied in medical, food, and chemical industries, sensitive thiourea detection remains a challenging and noticeable topic. Surface-enhanced Raman spectroscopy assay based on an enzyme-inhibition strategy is promising for the detection of thiourea residues. Therefore, in this study, Ag/CeO 2 nanomaterials with dual enzyme-Like Activities were synthesized to detection thiourea. We discovered that the Ag/CeO 2 nanozymes demonstrated strong peroxidase-like activity and oxidase-like activity, moreover they can accelerate and catalyze 3,3′,5,5′-tetramethylbenzidine enzymatic reaction , with significantly increasing the Raman signal. Instead, once thiourea was introduced into Ag/CeO 2 nanozymes, due to the influence of thiourea molecules occupation and nanozymes aggregation, the peroxidase-like activity and oxidase-like activity of Ag/CeO 2 decreased sharply, and the colorless substrate 3,3′,5,5′-tetramethylbenzidine would not undergo color changes. Based on this observation, a Raman method for detecting thiourea using oxidized 3,3′,5,5′-tetramethylbenzidine as a probe molecule was developed. The Raman intensity showed a good linearity for the concentration of thiourea in the range of 10 −11 M−10 −1 M with a correlation coefficient of R 2 = 0.9881, and the limit of detection is 2.4 × 10 −13 M. Therefore, the proposed Raman mode sensors exhibited excellent selectivity and anti-interference ability which offers a new avenue for exploiting environmental monitoring.
研究领域:
分析化学
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环境科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Leikai Zhao,Rui Zhang,Guixin Yang,Yuhang Wang,Shili Gai,Xin Zhao,Mengmeng Huang,Piaoping Yang
Foreseen as foundational in forthcoming oncology interventions are multimodal therapeutic systems. Nevertheless, the tumor microenvironment (TME), marked by heightened glucose levels, hypoxia, and scant concentrations of endogenous hydrogen peroxide could potentially impair their effectiveness. In this research, two-dimensional (2D) Ti3C2 MXene nanosheets are engineered with CeO2 nanozymes and glucose oxidase (GOD), optimizing them for TME, specifically targeting cancer therapy. Following our therapeutic design, CeO2 nanozymes, embodying both peroxidase-like and catalase-like characteristics, enable transformation of H2O2 into hydroxyl radicals for catalytic therapy while also producing oxygen to mitigate hypoxia. Concurrently, GOD metabolizes glucose, thereby augmenting H2O2 levels and disrupting the intracellular energy supply. When subjected to a near-infrared laser, 2D Ti3C2 MXene accomplishes photothermal therapy (PTT) and photodynamic therapy (PDT), additionally amplifying cascade catalytic treatment via thermal enhancement. Empirical evidence demonstrates robust tumor suppression both in vitro and in vivo by the CeO2/Ti3C2-PEG-GOD nanocomposite. Consequently, this integrated approach, which combines PTT/PDT and enzymatic catalysis, could offer a valuable blueprint for the development of advanced oncology therapies.
研究领域:
肿瘤学
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纳米医学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2023
·
IF 17.1
Bo Yuan,Zicong Tan,Qiang Guo,Xiutong Shen,Chao Zhao,Jian Lin Chen,Yung-Kang Peng
Nanozymes are promising alternatives to natural enzymes, but their use remains limited owing to poor specificity. For example, CeO2 activates H2O2 and displays peroxidase (POD)-like, catalase (CAT)-like, and haloperoxidase (HPO)-like activities. Since they unavoidably compete for H2O2, affecting its utilization in the target application, the precise manipulation of reaction specificity is thus imperative. Herein, we showed that one can simply achieve this by manipulating the H2O2 activation pathway on pristine CeO2 in well-defined shapes. This is because the coordination and electronic structures of Ce sites vary with CeO2 surfaces, wherein the (100) and (111) surfaces display nearly 100% specificity toward POD-/CAT-like and HPO-like activities, respectively. The antibacterial results suggest that the latter surface can well-utilize H2O2 to kill bacteria (cf., the former), which is promising for anti-biofouling applications. This work provides atomic insights into the synthesis of nanozymes with improved activity, reaction specificity, and H2O2 utilization.
研究领域:
生物化学
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材料科学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuxuan Dai,Shenghan Gao,Zhi Zhang,Wan-Jie Du,Xinyu Bao,Yun Xu
Alzheimer's disease (AD) has displayed complex pathological features, in which oxidative stress and ferroptosis play important roles. The development of collaborative treatment methods aimed at multiple pathological factors is particularly important for effective alleviation of AD. In our research, a multifunctional delivery system (CART-CeO 2 )@HA was developed for the co-delivery of neuroprotective Cocaine- and amphetamine-regulated transcript (CART) peptides and antioxidant CeO 2 nanozymes encapsulated within an ROS-responsive nanogel. Under the stimulation of Aβ, (CART-CeO 2 )@HA exhibited effective relief for cell apoptosis, mitochondrial dysfunction and synaptic loss on account of ROS removal and ferrous ions chelation. With sufficient accumulation and sustained drug release in the brain of APP/PS1 mice, (CART-CeO 2 )@HA significantly reduced the levels of oxidative stress and ferroptosis, which facilitate the reduction of Aβ deposition and the repair of neuronal damage, jointly rescuing the cognitive impairment in APP/PS1 mice. These results verified the clinical potential of the multi-target delivery system in AD synergistic therapy.
研究领域:
神经科学
·
纳米技术
·
药剂学
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生物医学工程
·
病理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-15
·
IF 10.6
Sun Yuxiang,Xu Tianze,Qian Yike,Chen Qiaoyun,Xiong Fei,Du Wenxian,Xu Li
Ceria nanoparticles (CeO 2 NPs) exhibit great potential in cardiovascular disease and nonalcoholic fatty liver disease due to its excellent antioxidant capacity. However, the profitable effect of CeO 2 NPs on many diseases is almost all attributed to the regulation of ROS. Apart from the general antioxidant function, there seems to be no more distinct mechanism to reflect its unique multi-disease improvement effect. Here, we for the first time reveal a new discovery of CeO 2 NPs in mimicking nitric oxide synthase (NOS) by catalyzing L-arginine (L-Arg) to produce nitric oxide (NO) or the derivatives. NOS-like activity of CeO 2 NPs is original and associated with multiple factors like substrate concentration, pH, temperature and time, etc. where oxygen vacancy ratio plays a more critical role. Meanwhile, NOS-like activity of CeO 2 NPs successfully elevates NO secretion in endothelial cells and macrophages without expanding eNOS/iNOS expression. Importantly, NOS-like activity of CeO 2 NPs and the responsive endogenous NO promote the re-distribution of blood lipids and stabilize eNOS expression but suppress iNOS, thus collectively alleviate the accumulation of vascular plaque. Altogether, we provide a new angle of view to survey the outstanding potential of CeO 2 NPs, apart from the inevitable antioxidant capacity, the covert but possible and more critical NOS-like enzymatic activity is more noteworthy.
研究领域:
生物医学工程
·
心血管疾病研究
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Shengnan Zhang,Hongxian Zhou,Xin Liu,Yantong Wu,Shuran Yang,Jia Yin,Mohammad H. Shubair,Mingzhu Zhang
Periodontitis, a chronic inflammatory disease, leads to the progressive destruction of tooth-supporting tissues, and current treatments fall short of fully restoring periodontal regeneration. Dental pulp stem cells (DPSCs) have shown great promise for periodontal regeneration due to their ability to differentiate into multiple cell types and modulate immune responses. However, clinical use of DPSCs remains challenging due to poor cell retention, dependence on scaffolds, and high costs. Additionally, excessive reactive oxygen species (ROS) in the periodontal microenvironment hinder stem cell proliferation and differentiation, further limiting their regenerative potential. To address these challenges, we developed ultrafine cerium oxide nanoparticles (CeO2NPs) and engineered DPSCs (E-DPSCs) by incorporating these antioxidant nanozymes via endocytosis. The modified E-DPSCs exhibited enhanced proliferation, migration, and osteogenic differentiation under normal conditions. Crucially, under oxidative stress, E-DPSCs effectively neutralized ROS, reduced apoptosis, and maintained their osteogenic capacity. In vivo studies further demonstrated that E-DPSCs significantly enhanced periodontal regeneration compared to unmodified DPSCs. These findings highlight E-DPSCs as a promising solution to overcome current limitations in stem cell therapy, offering a more effective strategy for periodontal repair and regeneration.
研究领域:
牙科学
·
纳米医学
·
干细胞研究
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
·
2021
·
IF 11.556
Shaofang Zhang,Ying Liu,Si Sun,Junying Wang,Qifeng Li,Ruijuan Yan,Yalong Gao,Haile Liu,Shuangjie Liu,Wenting Hao,Haitao Dai,Changlong Liu,Yuanming Sun,Wei Long,Xiaoyu Mu,Xiao-Dong Zhang
Traumatic brain injury (TBI) is a sudden injury to the brain, accompanied by the production of large amounts of reactive oxygen and nitrogen species (RONS) and acute neuroinflammation responses. Although traditional pharmacotherapy can effectively decrease the immune response of neuron cells via scavenging free radicals, it always involves in short reaction time as well as rigorous clinical trial. Therefore, a noninvasive topical treatment method that effectively eliminates free radicals still needs further investigation. Methods: In this study, a type of catalytic patch based on nanozymes with the excellent multienzyme-like activity is designed for noninvasive treatment of TBI. The enzyme-like activity, free radical scavenging ability and therapeutic efficacy of the designed catalytic patch were assessed in vitro and in vivo . The structural composition was characterized by the X-ray diffraction, X-ray photoelectron spectroscopy and high-resolution transmission electron microscopy technology. Results: Herein, the prepared Cr-doped CeO 2 (Cr/CeO 2 ) nanozyme increases the reduced Ce 3+ states, resulting in its enzyme-like activity 3-5 times higher than undoped CeO 2 . Furthermore, Cr/CeO 2 nanozyme can improve the survival rate of LPS induced neuron cells via decreasing excessive RONS. The in vivo experiments show the Cr/CeO 2 nanozyme can promote wound healing and reduce neuroinflammation of mice following brain trauma. The catalytic patch based on nanozyme provides a noninvasive topical treatment route for TBI as well as other traumas diseases. Conclusions: The catalytic patch based on nanozyme provides a noninvasive topical treatment route for TBI as well as other traumas diseases.
研究领域:
神经科学
·
纳米技术
·
药理学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 38
·16349-16361
·
IF 7.79
Shuoshuo Yang,Jiale Ji,Mengwei Luo,Hailing Li,Zhonghong Gao
Ceria (CeO2) based materials possess many antioxidant enzyme-like activities and unique properties for bone repair, but their free radical scavenging function is still insufficient. In order to deal with the complex oxidative stress environment in bone repair, multifunctional composite CeO2 nanozymes (CeO2NZs), featuring multiple antioxidative properties, were constructed via surface modification on CeO2NZs with nanoscale poly(tannic acid) (PTA) coatings. Moreover, we adjusted pH conditions (ranging from 4 to 9) to effectively control the formation and antioxidative properties of PTA coatings on CeO2NZ surfaces. Here, the physical properties of this novel inorganic and organic composite antioxidant, such as surface morphology, particle size, crystal structure, surface charge and element composition, were thoroughly characterized. The PTA/CeO2NZs showed obvious coating morphology under weak acid conditions (pH = 5–6), and the PTA layer at pH = 5 is about 1 nm in thickness. Compared with untreated CeO2NZs, the PTA/CeO2NZs showed stronger SOD-like activity and obviously higher free radical scavenging rate (for both ABTS+˙ and DPPH˙).Notably, this composite antioxidative nanozyme not only exhibited favorable cell proliferation of preosteoblasts (MC3T3-E1) but also provided strong antioxidative property to maintain cell vitality against H2O2 induced oxidative damage. In particular, this study provides new insights into the designing of surface polyphenolic coatings at the nanoscale, and these multiple antioxidative properties shown by PTA coated CeO2NZs make them suitable for protecting cells under the oxidative stress environment.
研究领域:
材料科学
·
纳米技术
·
生物材料
·
细胞生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）