JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Mingjie Xu,Jianheng Guo,Tengjie Li,Chunmei Zhang,Xue Peng,Ke Xing,Sheng Qin
Black spot disease caused by Ceratocystis fimbriata is destructive to the production, transportation, and storage of sweet potato. The antifungal effects of Bacillus tequilensis XK29 against C. fimbriata through volatile organic compounds (VOCs) were evaluated in this study. The activated carbon assay proved that XK29 could exert antibiotic effects through volatiles. By optimizing the wheat seed weight, inoculation method, concentration, volume, and time, the antifungal activity of XK29 was significantly improved. XK29 fumigation inhibited spore formation and germination and changed the cell morphology of C. fimbriata. During the storage of sweet potato tuber roots, XK29 effectively controlled black spot disease and reduced the weight loss and malondialdehyde content. Metabolomic analysis revealed that 21 volatile compounds were released from XK29. Isovaleric acid, isobutyric acid, and 2-methylbutanoic acid effectively inhibited the growth of C. fimbriata. These results indicate that B. tequilensis XK29 has a good potential to be developed as a microbial fumigation agent.
研究领域:
植物病理学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Xuezhi Li,Man Liu,Tinggong Huang,Kunlong Yang,Sihan Zhou,Yongxin Li,Jun Tian
Ceratocystis fimbriata is the most devastating phytopathogen causing significant losses in post-harvest sweet potato. In this study, monoterpene nerol (NEL), the active compound in neroli essential oil, was found to dose-dependently inhibit the mycelial growth and spore germination of C. fimbriata at a minimum inhibitory concentration (MIC) of 0.25 mL L -1 . NEL vapor treatments significantly reduced the incidence and lesion diameter of black rot in sweet potato infected by the fungus and regulated the defense-related enzyme activity of phenylalanine ammonia lyase (PAL). Using RNA sequencing (RNA-seq) and biochemical assays, it was demonstrated that NEL treatment impaired cell membrane integrity via down-regulating the expression of ergosterol synthesis genes and reduced the ergosterol content. Moreover, an analysis of a series of apoptotic events revealed that NEL treatment caused mitochondrial membrane damage by reducing the mitochondrial membrane potential (MMP, Δψm), which led to down-regulation of genes involved in ATP production, then induced accumulation of intracellular reactive oxygen species (ROS) generation. Simultaneously, NEL caused nuclear chromatin condensation and concomitant DNA cleavage, which led to the up-regulation of DNA repair genes expression, and the cell-cycle arrest principally occurred at the G2/M phase in C. fimbriata . Altogether, these findings provide information about the underlying antifungal mechanism of NEL against C. fimbriata and suggest that NEL could be a useful alternative for controlling C. fimbriata in post-harvest spoilage of sweet potato.
研究领域:
植物病理学
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分子生物学
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微生物学
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植物病原学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
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IF 3.571
Yu Zhang,Tengjie Li,Yuanfang Liu,Xiaoyan Li,Chunmei Zhang,Zhaozhong Feng,Xue Peng,Zongyun Li,Sheng Qin,Ke Xing
The biocontrol activity and chemical composition of the volatile organic compounds (VOCs) produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 were investigated. The VOCs inhibited mycelial growth and spore germination in Ceratocystis fimbriata, which causes black rot disease in sweet potato tuber roots (TRs) and showed wide-spectrum antifungal activity against several plant pathogenic fungi. A microscopic examination of C. fimbriata cells suggested morphological changes and a loss of cellular contents. Different inoculation strategies significantly affected the antifungal activity of the VOCs. In the volatile profile of SPS-41, the most abundant compound, 3-methyl-1-butanol, followed by phenylethyl alcohol and 2-methyl-1-butanol showed strong inhibition toward C. fimbriata. The weight loss rate and disease severity of the TRs were significantly reduced in response to the VOCs emitted by SPS-41. The results suggest that the VOCs produced by P. chlororaphis subsp. aureofaciens SPS-41 might constitute an attractive biological fumigant for controlling black rot disease in sweet potato TRs.
研究领域:
植物病理学
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微生物学
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DOI
PubMed
高影响因子
Microorganisms
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2020
·Vol. 8
·期 3
·319
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IF 4.152
Xuewei Li,Beibei Li,Shurui Cai,Yu Zhang,Mingjie Xu,Chunmei Zhang,Bo Yuan,Ke Xing,Sheng Qin
Black spot disease, which is caused by the pathogenic fungalCeratocystis fimbriata, seriously affects the production of sweet potato and its quality during postharvest storage. In this study, the preliminary identification of the rhizosphere actinomycete strain SPS-33, and its antifungal activity of volatilesin vitroandin vivowas investigated. Based on morphological identification and phylogenetic analysis of the 16S rRNA gene sequence, strain SPS-33 was identified asStreptomyces lavendulae.Volatile organic compounds (VOCs) emitted by SPS-33 inhibited mycelial growth and sporulation ofC. fimbriatain vitroand also induced a series of observable hyphae morphological changes. In anin vivopathogenicity assay, exposure to SPS-33 significantly decreased the lesion diameter and water loss rate in sweet potato tuberous roots (TRs) inoculated withC. fimbriata. It increased the antioxidant enzymes’ activities of peroxidase, catalase, and superoxide dismutase as well as decreased malondialdehyde and increased total soluble sugar. In the VOC profile of SPS-33 detected by a headspace solid-phase micro extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS), heptadecane, tetradecane, and 3-methyl-1-butanol were the most abundant compounds. 2-Methyl-1-butanol, 3-methyl-1-butanol, pyridine, and phenylethyl alcohol showed strong antifungal effects againstC. fimbriata. These findings suggest that VOCs fromS. lavendulaeSPS-33 have the potential for pathogenC. fimbriatacontrol in sweet potato postharvest storage by fumigant action.Keywords:Ipomoea batatas(L.) Lam.;Streptomyces lavendulae;volatile organic compound;fumigant;antimicrobial
研究领域:
植物病理学
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微生物学
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产品关联
DOI
PubMed
高影响因子
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