PLoS One
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2017
·Vol. 12
·期 7
·e0181094
·
IF 2.806
Qi Sun,Jiajin Zhu,Feiwei Cao,Fengjia Chen
Chimonanthus nitens Oliv. (CN) is a species in the family Calycanthaceae. Its leaf is widely used to make traditional herbal tea in southern China and has a wide range of therapeutic effects. The profile of the ethanol extracts from CN leaves was identified by UPLC-QTOF-MS/MS. Forty seven compounds were determined including organic acids, phenolic acids and derivatives, flavonoids, coumarins, fatty acids and other compounds. The effect of the CN extracts on the inflammatory damage in zebrafish and in RAW 264.7 cells was investigated. The extracts demonstrated a strong ability to inhibit the recruitment of neutrophils in LPS-stimulated zebrafish, but macrophage migration was not significantly affected. Pro-inflammatory cytokines (i.e., TNF-α, IL-6 and IL-1β) were also determined by q-PCR. The extracts strongly reduced mRNA expression of TNF-α, IL-6 but not IL-1β in zebrafish model, while significantly inhibited the production of the factors in the RAW 264.7 cells. Therefore, our results suggest that the ethanol extracts of CN leaves may serve as a source of nutraceutical compounds with anti-inflammatory properties.
研究领域:
药理学
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免疫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
·
2023
·
IF 7.31
Yang Zhang,Yantian Tang,Lei Cai,Jing He,Lingli Chen,Kehui Ouyang,Wenjun Wang
To investigate the immunomodulatory activities of Chimonanthus nitens Oliv polysaccharides (COP1), an immunosuppressive mouse model was generated by cyclophosphamide (CY) administration and then treated with COP1. The results demonstrated that COP1 ameliorated the body weight and immune organ (spleen and thymus) index of mice and improved the pathological changes of the spleen and ileum induced by CY. COP1 strongly stimulated the production of inflammatory cytokines (IL-10, IL-12, IL-17, IL-1β, and TNF-α) of the spleen and ileum by promoting the mRNA expressions. Furthermore, COP1 had immunomodulatory activity by increasing several transcription factors (JNK, ERK, and P38) in the mitogen-activated protein kinase (MAPK) signaling pathway. Related to the above immune stimulatory effects, COP1 positively affected the production of short-chain fatty acids (SCFAs) and the expression of ileum tight junction (TJ) protein (ZO-1, Occludin-1, and Claudin-1), upregulated the level of secretory immunoglobulin A (SIgA) in the ileum and microbiota diversity and composition, and improved intestinal barrier function. This study suggests that COP1 may provide an alternative strategy for alleviating chemotherapy-induced immunosuppression.
研究领域:
免疫学
·
药理学
·
微生物学
·
分子生物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Min Zhang,Danna Fan,Wenjun Wang,Wenbing Hu,Yi Han,Lingli Chen,Suyun Lin,Chuanlong Yu,Min Wang,Hui Chen
CTF alleviated HFFD-induced insulin resistance by regulating the gut-muscle axis in mice. • Antibiotic-mediated microbiota depletion verified that gut microbiota acts as a critical mediator underlying the insulin-sensitizing effects of CTF. • CTF remodeled intestinal flora homeostasis and activated skeletal muscle AMPK/GLUT4/UCP1 signaling to restore glucose metabolic homeostasis. Insulin resistance (IR) drives obesity and type 2 diabetes, with defective skeletal muscle glucose metabolism as the primary trigger of systemic metabolic dysfunction. We previously confirmed the glycolipid-regulating activity of Chimonanthus nitens Oliv. leaves total flavonoids (CTF), yet its gut microbiota-dependent anti-insulin resistance mechanism remains unclear. In this study, five major flavonoid constituents of CTF were identified using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF MS/MS), namely rutin, isoquercitrin, kaempferol-3-O-rutinoside, astragalin and quercetin. We established a high-fat and high-fructose diet (HFFD)-induced IR mouse model to explore CTF's protective effects and molecular mechanisms through comprehensive detection of physiological parameters, gut microbial profiles, cecal metabolites and skeletal muscle function. Microbiologically, CTF relieved glucose disorders and IR by restoring gut microbial and cecal metabolite homeostasis. Antibiotics-mediated gut microbiota depletion nearly abolished hypoglycemic and muscle-protective capacities of CTF, demonstrating gut microbiota as an indispensable mediator of CTF efficacy. Mechanistically, CTF activated adenosine monophosphate-activated protein kinase (AMPK) signaling, thereby synchronously upregulating glucose transporter 4 (GLUT4) and uncoupling protein 1 (UCP1). Specifically, CTF raised skeletal muscle glycogen accumulation and succinate dehydrogenase (SDH) activity, enhanced GLUT4 expression and its translocation onto the sarcolemma to accelerate muscular glucose uptake. Meanwhile, the upregulated UCP1 promoted thermogenesis of intermuscular fat and further alleviated lipotoxic damage. In conclusion, CTF alleviates HFFD-induced IR predominantly by regulating gut microbiota and cecal metabolism, and recovers skeletal muscle glucose homeostasis through the gut-muscle-axis-dependent AMPK/GLUT4/UCP1 pathway. This research offers a theoretical basis for CTF as natural anti-metabolic disorder dietary supplements. Download: Download high-res image (336KB) Download: Download full-size image
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高影响因子
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