JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2016
·Vol. 54
·期 7
·1213-1219
·
IF 1.32
Liang Xianrui,Zhao Cui,Su Weike
A novel fingerprinting approach was developed by means of ultra-high-performance liquid chromatography with photodiode array detector (UPLC-PAD) for the quality control of Chimonanthus salicifolius (C. salicifolius). All UPLC analyses were carried out on a Waters Acquity BEH Phenyl column (2.1 × 50 mm, 1.7 μm particle size) at 48°C, with a gradient mobile phase composed of 0.1% aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 mL/min. The method validation results demonstrated the developed method possessing desirable precision [<0.88% relative standard deviation (RSD)], reproducibility (<1.87% RSD), stability (<1.42% RSD) and allowing fingerprint analysis in one chromatographic run within 21 min. The quality assessment was achieved by using chemometrics methods including similarity analysis, hierarchical clustering analysis and principal component analysis. The developed method can be used for further quality control of C. salicifolius.
研究领域:
植物学
·
药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yijie Chen,Liang Hong,Qifang Wu,Meiyan Sun,Menglai Ling,Haibin Tong,Zhengshuang Liu
Ethnopharmacological relevance Chimonanthus salicifolius S. Y. Hu (Salicaceae), known as "Liu Ye La Mei," is traditionally used by the She ethnic minority in China to treat respiratory ailments including cough, asthma, and bronchitis. This ethnobotanical background provides a strong rationale for investigating its potential therapeutic value in acute lung injury (ALI). Aim of the study : This study aimed to evaluate the protective effects of the essential oil from Chimonanthus salicifolius (CSEO) against lipopolysaccharide (LPS)-induced ALI in mice and to elucidate the underlying mechanisms. Methods ALI was induced in C57BL/6 mice by intratracheal LPS instillation, with separate cohorts pretreated intraperitoneally with CSEO or its component eucalyptol. In vivo therapeutic efficacy was evaluated by assessing lung histopathology, pulmonary edema, BALF inflammatory markers, and neutrophil activation status. In vitro , the effects of CSEO and eucalyptol on key neutrophil functions were assessed, including adhesion to ICAM-1 and chemotaxis (Transwell, Zigmond assays). The mechanism was investigated by evaluating interference with the β2 integrin/ICAM-1 interaction using competitive ELISA and co-immunoprecipitation. Following the identification of eucalyptol as a lead candidate via molecular docking, direct eucalyptol-β2 integrin binding was confirmed using multiple biophysical methods (CETSA, DARTS, MST). Results CSEO administration significantly ameliorated LPS-induced lung pathology, pulmonary edema, inflammatory cell infiltration, and suppressed MPO/NE activity and ROS levels in lung tissues. CSEO also reduced the production of pro-inflammatory cytokines and inhibited NF-κB pathway activation in the lungs. In vitro , CSEO dose-dependently inhibited neutrophil adhesion to ICAM-1 and chemotaxis towards CXCL1. Mechanistically, CSEO directly disrupted the binding between β2 integrin and ICAM-1 in both competitive ELISA and protein binding assays. Molecular docking identified eucalyptol as the CSEO constituent with the highest predicted binding affinity for β2 integrin. Subsequent experiments confirmed that eucalyptol significantly inhibited β2 integrin/ICAM-1 binding and suppressed neutrophil adhesion and chemotaxis in vitro . Biophysical assays provided strong evidence for a direct interaction between eucalyptol and β2 integrin, with MST determining a dissociation constant (Kd) of approximately 19.5 μM. Importantly, in vivo administration of eucalyptol replicated the protective effects of CSEO, significantly mitigating ALI severity, reducing inflammation, and inhibiting neutrophil recruitment and activation markers. Conclusions CSEO demonstrates significant therapeutic potential against ALI by inhibiting neutrophil trafficking. Its key component, eucalyptol, directly antagonizes the β2 integrin/ICAM-1 interaction, leading to reduced neutrophil adhesion and chemotaxis. This work provides scientific evidence for the traditional use of C. salicifolius and identifies eucalyptol as a promising β2 integrin-targeting agent for treating ALI.
研究领域:
药理学
·
免疫学
·
民族药理学
·
肺病学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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