ENVIRONMENTAL POLLUTION
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2023
·
IF 8.9
Kai Zhao,Xun Zhao,Tianbo Gao,Xuan Li,Guanqi Wang,Xinxiang Pan,Junsheng Wang
A novel dielectrophoresis (DEP)-assisted device for the bioremediation of heavy metal ions by using Chlorella microalgae is presented in this paper. To generate the DEP forces, pairs of electrode mesh were inserted in the DEP-assisted device. By applying DC electric field via the electrodes, the inhomogeneous electric field gradient is induced and the strongest non-uniform electric field exists near the mesh cross-corner. After the adsorption of Cd and Cu heavy metal ions by Chlorella , the Chlorella chain were trapped along the vicinity of the electrode mesh. Then, the effects of Chlorella concentration on the adsorption of heavy metal ions, and the applied voltage and electrode mesh size on the removal of Chlorella are conducted. In the co-existing Cd and Cu solutions, the individual adsorption ratio of Cd and Cu reaches as high as approximately 96% and 98%, respectively, showing excellent bioremediation capability of multiple heavy metal ions in wastewater. By adjusting the applied electric voltage and the mesh size, the Chlorella adsorbed with Cd and Cu are captured by negative DC-DEP effects and the removal ratio of Chlorella reach an average of 97%, providing a method for the removal of multiple heavy metal ions in wastewater by using Chlorella microalgae.
研究领域:
环境科学
·
生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Huan Li,Yun Zhang,Jiuyi Liu,Zhensheng Shen,An Li,Tian Ma,Qian Feng,Yingqiang Sun
The aim of this work was to study the biotreatment of mixed wastewaters collected from two points of MnO 2 industry by Chlorella vulgaris . Their growth rates in four mixed wastewaters with mass ratio of wastewater 1 # :2 # of 20:1, 50:1, 100:1, and 200:1 were characterized, and the lag phase was shortened with increase of nitrate concentrations. The N, P, and metal removal kinetics were quantified each other day to evaluate the bio-treatment efficiencies of high-nitrate wastewaters from MnO 2 industry. 84.68% and 98% of N, P has been removed. The Ca, Zn, Mn, and Si in mixed wastewaters was removed with maximum removal efficiencies of 97.91%, 99.37%, 99.44%, and 81.68%, respectively. The compositions of Chlorella vulgaris cultured in mixed wastewaters, including proteins, lipids, ash contents, and carbohydrates, were investigated in detail. The optimum HHV of Chlorella vulgaris about 18 MJ/Kg presented a potential to decrease the cost of algal biofuel.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2018
·
IF 5.807
Xinru Zhang,Hao Yuan,Zeyi Jiang,Dahao Lin,Xinxin Zhang
The organic matter and surfactants in wastewater may cause variations in the surface tension of wastewater (STW) ranging between ∼40 and ∼70 mJ·m −2 . This study focused on the influence of STW on microalgae biofilm formation. A theoretical analysis was first conducted, and then microalgae biofilm formation on hydrophilic and hydrophobic substrata in liquid and real wastewater with different surface tensions was studied. The results demonstrated that STW affected microalgae biofilm formation remarkably. When the surface tension of liquid medium ( γ lv ) was approximately equal to the average value of surface free energy of microalgae and substrata, biofilm formation reached the minimum. Microalgae biofilm formation on a hydrophilic surface first decreased (from ∼2200 to ∼1500 cells/mm 2 ) and then increased (from ∼1500 to 3100 cells/mm 2 ) with the decrease in γ lv (from ∼70 to ∼40 mJ·m −2 ), whereas biofilm on a hydrophobic surface continued to decrease (from ∼2500 to 1000 cells/mm 2 ).
研究领域:
环境生物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Haifeng Qian,Jiahui Xu,Tao Lu,Qi Zhang,Qian Qu,Zhaopeng Yang,Xiangliang Pan
Linoleic acid (LA), is the product of secondary metabolism secreted from Microcystis aeruginosa , and it exhibits allelopathic activity against eukaryotic algae. However, information about on the mechanisms associated with the inhibition of algal activity by LA is limited. In this study, Chlorella pyrenoidosa was treated with LA (20–120 μg L −1 ) for 4 days, and its growth inhibition and physiological responses were examined for potential toxic mechanisms. The photosynthetic efficiency of C. pyrenoidosa was inhibited by LA treatments, and the F v /F m parameter decreased significantly compared to that of controls; however, the photosynthetic pigment content did not change significantly. Peroxidase activity was enhanced, relieving oxidative damage in algae after LA treatments. However, superoxide dismutase and catalase were suppressed, ultimately leading to the aggravation of lipid peroxidation. Transcriptome-based gene expression analysis revealed that the 120 μg L −1 LA treatment significantly inhibited the transcription of genes related to photosynthesis, carbon metabolism, and amino acid metabolism in C. pyrenoidosa , suggesting that these genes might be key LA targets in C. pyrenoidosa . Moreover, the expression of genes involved in vitamin, lipid, nitrogen cycling, terpenoid, and ascorbate metabolism was also affected, suggesting that LA inhibits algal cell growth through multiple pathways. The identification of LA-responsive genes in C. pyrenoidosa provides new insight into LA stress responses in eukaryotic algae.
研究领域:
植物学
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生态学
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分子生物学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
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IF 11.2
Hao Tang,Jinwen Huang,Qingxia Yuan,Kunling Lv,Haiqiong Ma,Tingting Li,Yonghong Liu,Shunli Mi,Longyan Zhao
Chlorella is one of the most widely cultivated species of microalgae and has been consumed as a “green healthy food”. In this study, a novel polysaccharide (CPP-1) was isolated from Chlorella pyrenoidosa , structurally analyzed, and sulfated as a promising anticoagulant . Structural analyses by chemical and instrumental methods such as monosaccharide composition , methylation-GC–MS and 1D/2D NMR spectroscopy analysis revealed that CPP-1 had a molecular weight of ~13.6 kDa, and mainly consisted of d -mannopyranose ( d -Man p ), 3- O -methylated d -Man p (3- O -Me- d -Man p ), and d -galactopyranose ( d -Gal p ). The molar ratio of d -Man p and d -Gal p was 1.0:2.3. CPP-1 consisted of a (1→6)-linked β- d -Gal p backbone substituted at C-3 by the d -Man p and 3- O -Me- d -Man p residues in a molar ratio of 1:1, which was a regular mannogalactan. The sulfated Chlorella mannogalactan (SCM) with sulfated group content of 40.2 % equivalent to that of unfractionated heparin was prepared and analyzed. NMR analysis confirmed its structure, indicating that most free hydroxyl groups in the side chains and partial hydroxyl groups in the backbone were sulfated. Anticoagulant activity assays indicated that SCM exhibited strong anticoagulant activity by inhibiting intrinsic tenase (FXase) with IC 50 of 13.65 ng/mL, which may be a safer anticoagulant as an alternative to heparin-like drugs.
研究领域:
生物化学
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药理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2017
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IF 5.099
Tao Lu,Youchao Zhu,Jiahui Xu,Mingjing Ke,Meng Zhang,Chengxia Tan,Zhengwei Fu,Haifeng Qian
The top-selling strobilurin, azoxystrobin (AZ), is a broad-spectrum fungicide that protects against many kinds of pathogenic fungi by preventing their ATP production. The extensive use of AZ can have negative consequences on non-target species and its effects and toxic mechanisms on algae are still poorly understood. In this work, Chlorella pyrenoidosa that had been grown in BG-11 medium was exposed to AZ (0.5–10 mg L −1 ) for 10 d. The physiological and molecular responses of the algae to AZ treatment, including photosynthetic efficiency, lipid peroxidation level, antioxidant enzyme activities , as well as transcriptome-based analysis of gene expression, were examined to investigate the potential toxic mechanism. Results shows that the photosynthetic pigment (per cell) increased slightly after AZ treatments, indicating that the photosystem of C. pyrenoidosa may have been strengthened. Glutathione and ascorbate contents were increased, and antioxidant enzyme activities were induced to relieve oxidative damage (e.g., from lipid peroxidation) in algae after AZ treatment. Transcriptome-based analysis of gene expression combined with physiological verification suggested that the 5 mg L −1 AZ treatment did not inhibit ATP generation in C. pyrenoidosa , but did significantly alter amino acid metabolism, especially in aspartate- and glutamine-related reactions. Moreover, perturbation of ascorbate synthesis, fat acid metabolism, and RNA translation was also observed, suggesting that AZ inhibits algal cell growth through multiple pathways. The identification of AZ-responsive genes in the eukaryotic alga C. pyrenoidosa provides new insight into AZ stress responses in a non-target organism.
研究领域:
环境科学
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毒理学
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植物生理学
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DOI
PubMed
高影响因子
Water
·
2023
·Vol. 15
·期 20
·3544
·
IF 3.4
Qingnan Sun,Xiaoping Zhang,Xin Zhang
In the treatment of wastewater containing only chemical oxygen demand (COD) byChlorellasp., the cell activity and proliferation ability ofChlorellasp. decreased with the culture time, which affected the removal of COD in wastewater. To solve these problems, theChlorellasp.–natural microorganism symbiosis system was prepared. The system was used to explore how natural microorganisms affect the cell activity and the proliferation ability ofChlorellasp. in wastewater. In the treatment of COD byChlorellasp., the removal rate of COD decreased from 45.47% to 28.88%, with a decrease in the cell activity and proliferation ability ofChlorellasp. In theChlorellasp.–natural microorganism symbiotic system, the removal rate of COD reached 45.75%. With the introduction of natural microorganisms, the circulation of CO2and O2betweenChlorellasp. and natural microorganisms promoted photosynthesis and respiration, which enhanced the cell activity ofChlorellasp. Under the condition that the dosage of natural microorganisms was between 1% and 6%, the concentration ofChlorellasp. was close to the logarithmic growth phase, which maintained the proliferation ability ofChlorellasp. At the same time, the natural microorganisms grew and proliferated in wastewater containing only COD through preying onChlorellasp.Keywords:Chlorellasp.;natural microorganisms;symbiosis system;cell activity;predation
研究领域:
环境生物学
·
废水处理
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DOI
高影响因子
Materials Research Express
·
2019
·Vol. 6
·期 9
·0950c3
·
IF 1.449
Qianzhu Mu,Yanfeng Sun,Aiying Guo,Xiazhao Yu,Xiaoyue Xu,Aijun Cai,Xiuping Wang
A series of Fe3O4–TiO2 composites were successfully synthesized using Chlorella pyrenoidosa as a bio-template. C. pyrenoidosa is not only a promising bio-template for obtaining Fe3O4 and TiO2 nanoparticles (NPs) but is also a natural dye sensitizer source that improves the photocatalytic activity of the TiO2 NPs under visible light. The bandgap energy of Fe3O4–TiO2 is narrower than that of TiO2 due to the coupling between the Fe3O4 and TiO2 NPs. The coupling and dye-sensitization lead to the rapid separation and suppressed recombination of electron-hole pairs in Fe3O4–TiO2, which enhances the photocatalytic activity of TiO2 under visible light. The photocatalytic activity of the as-prepared composites was evaluated by degrading a rhodamine B (RhB) aqueous solution under visible-light irradiation. Fe3O4–TiO2 exhibits a high degradation efficiency for RhB, and can be withdrawn from solution easily using an external magnetic field.
研究领域:
材料科学
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环境科学
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生物学
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产品关联
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2023
·
IF 5.1
Zhenyu Zhang,Li Wang,Yinqiang Wu,Chenshuo Li,Pengcheng Fu,Jing Liu
Microalgae , particularly Chlorella , have promising selenium (Se) enrichment capacity and great potential in wastewater treatment and the development of nutraceuticals. To acquire Chlorella strains with strong heterotrophic propensity and robust Se tolerance, this research utilized a heterotrophic isolation strategy along with a high Se concentration medium as selection factors. The combined sequence of the ribosomal small subunit (SSU) and internal transcribed spacer (ITS) was utilized for phylogenetic analysis , and comparative analysis of ITS secondary structures was also integrated to identify the desired strain. A novel subtropical microalgal strain, Chlorella vulgaris HNUFU001 with high Se tolerance, was isolated from water samples in a lake at Hainan University, China. Gaussian process regression was utilized to assess the strain's growth under varying Se concentrations. The intracellular accumulation and extracellular removal of Se were measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) and Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES), respectively. The maximum intracellular organic Se (92 % of total Se) was observed as 108.835 μg/g dry weight (DW) for 1 mg/L Se treatment. The highest Se removal rate was 40 %. The high Se tolerance and enrichment traits enable Chlorella vulgaris HNUFU001 to be a promising candidate for the heterotrophic production of organic Se-related nutraceuticals.
研究领域:
环境科学
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微生物学
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生物技术
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 2
·1501-1512
·
IF 3.9
Dingyong Liu,Hongjie Cai,Weiming Zhou,Dandan Lei,Changlin Cao,Xinshu Xia,Liren Xiao,Qingrong Qian,Qinghua Chen
Photocatalysis is widely acknowledged as an efficient and environmentally friendly method for treating dye-contaminated wastewater. However, the utilization of powdered photocatalysts presents significant challenges, including issues related to recyclability and the potential for secondary pollution. Herein, a novel technique based on 3D printing for the synthesizing of iron oxide (Fe2O3) involving chlorella was presented. Initially, chlorella powders were immobilized within acrylonitrile butadiene styrene (ABS) and thermoplastic polyurethane (TPU) substrate plastics using melt extrusion technology. Subsequently, these composite materials were transformed into ABS/TPU/chlorella skeletons (ATCh40), through fused deposition molding (FDM) technology. The integration of Fe2O3 onto the ATCh40 (ATCh40-Fe2O3) skeletons was accomplished by subjecting them to controlled heating in an oil bath. A comprehensive characterization of the synthesized materials confirms the successful growth of Fe2O3 on the surface of 3D skeletons. This strategy effectively addresses the immobilization challenges associated with powdered photocatalysts. In photocatalytic degradation experiments targeting methyl orange (MO), the ATCh40-Fe2O3 skeletons exhibited a remarkable MO removal rate of 91% within 240 min. Under conditions where the pH of MO solution was maintained at 3, and the ATCh40-Fe2O3 skeletons were subjected to a heat treatment in a 150 °C blast drying oven for 2 hours, the degradation rate of MO remained substantial, achieving 90% removal after 6 cycles. In contrast, when the same synthetic procedure was applied to ABS/TPU (AT) skeletons, the resulting product was identified as α-FeOOH. The MO removal rate by the AT-α-FeOOH skeletons was considerably lower, reaching only 49% after 240 min. This research provided a practical approach for the construction of photocatalytic devices through the use of 3D printing technology.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shasha Zhao,Jin Qian,Bianhe Lu,Sijing Tang,Yuxuan He,Yin Liu,Yitong Yan,Shuai Jin
Microalgae-bacteria consortia (MBC) system has been shown to enhance the efficiency of microalgae in wastewater treatment, yet its effectiveness in treating levofloxacin (LEV) wastewater remains unexplored. This study compared the treatment of LEV wastewater using pure Chlorella pyrenoidosa (PA) and its MBC constructed with activated sludge bacteria. The results showed that MBC improved the removal efficiency of LEV from 3.50–5.41 % to 33.62–57.20 % by enhancing the growth metabolism of microalgae. The MBC increased microalgae biomass and extracellular polymeric substance (EPS) secretion, yet reduced photosynthetic pigment content compared to the PA. At the phylum level, Proteobacteria and Actinobacteriota are the major bacteria in MBC. Furthermore, the transcriptome reveals that the growth-promoting effects of MBC are associated with the up-regulation of genes encoding the glycolysis, the citrate cycle (TCA cycle), and the pentose phosphate pathway. Enhanced carbon fixation, coupled with down-regulation of photosynthetic electron transfer processes, suggests an energy allocation mechanism within MBC. The up-regulation of porphyrin and arachidonic acid metabolism, along with the expression of genes encoding LEV-degrading enzymes, provides evidence of MBC’s superior tolerance to and degradation of LEV. Overall, these findings lead to a better understanding of the underlying mechanisms through which MBC outperforms PA in treating LEV wastewater.
研究领域:
环境科学
·
微生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Juan Liu,Shiqing Sun,Kangyu Chen,Shumei Gao,Dixing Chen,Yuejin Zhang,Changwei Hu
Fungi-microalgae consortia have great potential in microalgae harvesting and wastewater treatment. However, the symbiotic mechanisms underlying diffusible signaling metabolite production by fungi and microalgae remain unclear. Here, the positive impacts of Clonostachys rosea on Chlorella sp. were investigated using metabolomics and transcriptomics during a 5-day non-contact co-culture experiment. Co-cultured Chlorella sp. had a significantly higher growth rate and better photosynthetic performance than its mono-culture equivalent. The metabolites played important roles in the up-regulation of energy and amino acid metabolism processes in algal cells, whilst transcriptomics showed the up-regulation of genes involved in photosynthesis and signaling molecules. The biosynthesis of tryptophan and indole-3-acetic acid (IAA) was up-regulated in both algal cells and fungi, indicating that IAA is an important diffusible signaling molecule for algal growth regulation. This work provides novel insights into the signaling molecule interactions between fungi and microalgae in fungi-microalgae consortia.
研究领域:
微生物学
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环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）