SCIENCE OF THE TOTAL ENVIRONMENT
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2018
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IF 4.61
Haifeng Qian,Jiahui Xu,Tao Lu,Qi Zhang,Qian Qu,Zhaopeng Yang,Xiangliang Pan
Linoleic acid (LA), is the product of secondary metabolism secreted from Microcystis aeruginosa , and it exhibits allelopathic activity against eukaryotic algae. However, information about on the mechanisms associated with the inhibition of algal activity by LA is limited. In this study, Chlorella pyrenoidosa was treated with LA (20–120 μg L −1 ) for 4 days, and its growth inhibition and physiological responses were examined for potential toxic mechanisms. The photosynthetic efficiency of C. pyrenoidosa was inhibited by LA treatments, and the F v /F m parameter decreased significantly compared to that of controls; however, the photosynthetic pigment content did not change significantly. Peroxidase activity was enhanced, relieving oxidative damage in algae after LA treatments. However, superoxide dismutase and catalase were suppressed, ultimately leading to the aggravation of lipid peroxidation. Transcriptome-based gene expression analysis revealed that the 120 μg L −1 LA treatment significantly inhibited the transcription of genes related to photosynthesis, carbon metabolism, and amino acid metabolism in C. pyrenoidosa , suggesting that these genes might be key LA targets in C. pyrenoidosa . Moreover, the expression of genes involved in vitamin, lipid, nitrogen cycling, terpenoid, and ascorbate metabolism was also affected, suggesting that LA inhibits algal cell growth through multiple pathways. The identification of LA-responsive genes in C. pyrenoidosa provides new insight into LA stress responses in eukaryotic algae.
研究领域:
植物学
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生态学
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2017
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IF 5.099
Tao Lu,Youchao Zhu,Jiahui Xu,Mingjing Ke,Meng Zhang,Chengxia Tan,Zhengwei Fu,Haifeng Qian
The top-selling strobilurin, azoxystrobin (AZ), is a broad-spectrum fungicide that protects against many kinds of pathogenic fungi by preventing their ATP production. The extensive use of AZ can have negative consequences on non-target species and its effects and toxic mechanisms on algae are still poorly understood. In this work, Chlorella pyrenoidosa that had been grown in BG-11 medium was exposed to AZ (0.5–10 mg L −1 ) for 10 d. The physiological and molecular responses of the algae to AZ treatment, including photosynthetic efficiency, lipid peroxidation level, antioxidant enzyme activities , as well as transcriptome-based analysis of gene expression, were examined to investigate the potential toxic mechanism. Results shows that the photosynthetic pigment (per cell) increased slightly after AZ treatments, indicating that the photosystem of C. pyrenoidosa may have been strengthened. Glutathione and ascorbate contents were increased, and antioxidant enzyme activities were induced to relieve oxidative damage (e.g., from lipid peroxidation) in algae after AZ treatment. Transcriptome-based analysis of gene expression combined with physiological verification suggested that the 5 mg L −1 AZ treatment did not inhibit ATP generation in C. pyrenoidosa , but did significantly alter amino acid metabolism, especially in aspartate- and glutamine-related reactions. Moreover, perturbation of ascorbate synthesis, fat acid metabolism, and RNA translation was also observed, suggesting that AZ inhibits algal cell growth through multiple pathways. The identification of AZ-responsive genes in the eukaryotic alga C. pyrenoidosa provides new insight into AZ stress responses in a non-target organism.
研究领域:
环境科学
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毒理学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
Materials Research Express
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2019
·Vol. 6
·期 9
·0950c3
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IF 1.449
Qianzhu Mu,Yanfeng Sun,Aiying Guo,Xiazhao Yu,Xiaoyue Xu,Aijun Cai,Xiuping Wang
A series of Fe3O4–TiO2 composites were successfully synthesized using Chlorella pyrenoidosa as a bio-template. C. pyrenoidosa is not only a promising bio-template for obtaining Fe3O4 and TiO2 nanoparticles (NPs) but is also a natural dye sensitizer source that improves the photocatalytic activity of the TiO2 NPs under visible light. The bandgap energy of Fe3O4–TiO2 is narrower than that of TiO2 due to the coupling between the Fe3O4 and TiO2 NPs. The coupling and dye-sensitization lead to the rapid separation and suppressed recombination of electron-hole pairs in Fe3O4–TiO2, which enhances the photocatalytic activity of TiO2 under visible light. The photocatalytic activity of the as-prepared composites was evaluated by degrading a rhodamine B (RhB) aqueous solution under visible-light irradiation. Fe3O4–TiO2 exhibits a high degradation efficiency for RhB, and can be withdrawn from solution easily using an external magnetic field.
研究领域:
材料科学
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环境科学
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生物学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Shasha Zhao,Jin Qian,Bianhe Lu,Sijing Tang,Yuxuan He,Yin Liu,Yitong Yan,Shuai Jin
Microalgae-bacteria consortia (MBC) system has been shown to enhance the efficiency of microalgae in wastewater treatment, yet its effectiveness in treating levofloxacin (LEV) wastewater remains unexplored. This study compared the treatment of LEV wastewater using pure Chlorella pyrenoidosa (PA) and its MBC constructed with activated sludge bacteria. The results showed that MBC improved the removal efficiency of LEV from 3.50–5.41 % to 33.62–57.20 % by enhancing the growth metabolism of microalgae. The MBC increased microalgae biomass and extracellular polymeric substance (EPS) secretion, yet reduced photosynthetic pigment content compared to the PA. At the phylum level, Proteobacteria and Actinobacteriota are the major bacteria in MBC. Furthermore, the transcriptome reveals that the growth-promoting effects of MBC are associated with the up-regulation of genes encoding the glycolysis, the citrate cycle (TCA cycle), and the pentose phosphate pathway. Enhanced carbon fixation, coupled with down-regulation of photosynthetic electron transfer processes, suggests an energy allocation mechanism within MBC. The up-regulation of porphyrin and arachidonic acid metabolism, along with the expression of genes encoding LEV-degrading enzymes, provides evidence of MBC’s superior tolerance to and degradation of LEV. Overall, these findings lead to a better understanding of the underlying mechanisms through which MBC outperforms PA in treating LEV wastewater.
研究领域:
环境科学
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微生物学
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分子生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Yu Feng,Ao Wang,Wenxian Fu,Donghui Song
Chlorella pyrenoidosa was exposed to nonylphenol (NP) to investigate the tolerance, antioxidant response, removal efficiency, and biodegradation mechanism. We conducted studies on algal biomass, chlorophyll a content, and photosynthetic activity, and found that C. pyrenoidosa exhibited a high tolerance even at 8 mg L −1 of NP. Changes in peroxidase (POD) and superoxide dismutase (SOD) activities indicated that the NP-induced oxidative stress caused oxidant damage, which increased the malondialdehyde (MDA) content. After culturing for 120 h, the NP removal efficiency of C. pyrenoidosa was 89%, 59%, 49%, and 48% in the 2, 4, 6, and 8 mg L −1 treatment groups, respectively. Degradation intermediates determined by GC–MS suggested that the biodegradation of NP in C. pyrenoidosa originated from the long alkyl chain. In addition, transcriptome analysis indicated that NP affected photosynthesis, antioxidase, and oxidoreductase activity-related genes. In summary, our results indicated that C. pyrenoidosa is a species that exhibits high tolerance and biodegradation capacity toward NP.
研究领域:
环境科学
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微生物学
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分子生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2022
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IF 11.889
Hutao Wang,Caiye Hu,Yi Wang,Chunji Jin,Zonglian She,Liang Guo
Sulfadiazine (SDZ) as a common sulfonamide antibiotic is frequently detected in wastewater, but there is little information on the high-value product recovery and toxicity tolerance evaluation of mixotrophic microalgae under SDZ stress. In this study, effects of SDZ on growth, photosynthesis, cellular damage, antioxidant capacity and intracellular biochemical components of Chlorella pyrenoidosa were investigated. Results showed that the growth of C. pyrenoidosa was inhibited by about 20% under high SDZ stress, but there was little impact on photosynthesis. Cellular damage and antioxidant capacity were evaluated using malondialdehyde (MDA) content and superoxide dismutase (SOD) activity to further explain the toxicity tolerance of mixotrophic microalgae. The SDZ stress not only increased lipid and carbohydrate content, respectively attaining to the maximum of 390.0 and 65.4 mg/L, but also improved the biodiesel quality of C. pyrenoidosa . The findings show the potential of mixotrophic microalgae for biodiesel production and wastewater treatment.
研究领域:
环境科学
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生物技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 14
·期 8
·449
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IF 3.4
Min Li,Zhenfang Shang,Yonglan Ma,Huijun Zhao,Zhijing Ni,Zhaojun Wei,Xiu Zhang
This study investigates the growth tolerance mechanisms ofChlorella pyrenoidosato 3-fluorophenol and its removal efficiency by algal cells. Our results indicate thatC. pyrenoidosacan tolerate up to 100 mg/L of 3-fluorophenol, exhibiting a significant hormesis effect characterized by initial inhibition followed by promotion of growth. InC. pyrenoidosacells, the activities of superoxide dismutase (SOD) and catalase (CAT), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), were higher than or comparable to the control group. Metabolic analysis revealed that the 3-fluorophenol treatment activated pathways, such as glycerol phospholipid metabolism, autophagy, glycosylphosphatidylinositol (GPI)-anchored protein biosynthesis, and phenylpropanoid biosynthesis, contributed to the stabilization of cell membrane structures and enhanced cell repair capacity. After 240 h of treatment, over 50% of 3-fluorophenol was removed by algal cells, primarily through adsorption. Thus,C. pyrenoidosashows potential as an effective biosorbent for the bioremediation of 3-fluorophenol.
研究领域:
环境科学
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生物技术
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微生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
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2021
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IF 8.001
Gul Muhammad,Ange Douglas Potchamyou Ngatcha,Yongkun Lv,Wenlong Xiong,Yaser A. El-Badry,Eylem Asmatulu,Jingliang Xu,Md Asraful Alam
This study investigates modeling and optimal conditions for biodiesel production from exceedingly wet microalgae Chlorella pyrenoidosa using the catalyst, hydrochloric acid . Three levels of Box-Behnken design response surface methodology were used to optimize individual and interactive effects of parameter time (120–240 min), temperature (120–160 °C), solvent-to-wet biomass ratio (2.0–4.67), and hydrochloric acid concentration (2–4 M). Temperature was the most significant factor for direct transesterification of wet microalgae (low p-value (0.0001) and high F-value (53.89). The highest yield (19.90%) of fatty acid methyl ester was obtained on dry biomass weight basis under the optimum conditions of 240 min, 146 °C, 2.83 (vol/wt), and 3.86 M acid concentration. The artificial neural network and response surface methodology were trained with Box-Behnken design data to predict responses, and to develop and compare each model's predictive abilities . The accuracy of results indicates that both models predict the experimental data for fatty acid methyl ester yields with high correlation coefficients (R 2 ) 0.94 and 0.92, respectively for artificial neural network and response surface methodology. The potential for producing biodiesel from C. pyrenoidosa is validated by the high yields of C18 fatty acid methyl esters. Experimental analysis demonstrated biodiesel quality in comparison with European and US standards.
研究领域:
生物工程
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可再生能源
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2023
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IF 11.2
Hao Tang,Jinwen Huang,Qingxia Yuan,Kunling Lv,Haiqiong Ma,Tingting Li,Yonghong Liu,Shunli Mi,Longyan Zhao
Chlorella is one of the most widely cultivated species of microalgae and has been consumed as a “green healthy food”. In this study, a novel polysaccharide (CPP-1) was isolated from Chlorella pyrenoidosa , structurally analyzed, and sulfated as a promising anticoagulant . Structural analyses by chemical and instrumental methods such as monosaccharide composition , methylation-GC–MS and 1D/2D NMR spectroscopy analysis revealed that CPP-1 had a molecular weight of ~13.6 kDa, and mainly consisted of d -mannopyranose ( d -Man p ), 3- O -methylated d -Man p (3- O -Me- d -Man p ), and d -galactopyranose ( d -Gal p ). The molar ratio of d -Man p and d -Gal p was 1.0:2.3. CPP-1 consisted of a (1→6)-linked β- d -Gal p backbone substituted at C-3 by the d -Man p and 3- O -Me- d -Man p residues in a molar ratio of 1:1, which was a regular mannogalactan. The sulfated Chlorella mannogalactan (SCM) with sulfated group content of 40.2 % equivalent to that of unfractionated heparin was prepared and analyzed. NMR analysis confirmed its structure, indicating that most free hydroxyl groups in the side chains and partial hydroxyl groups in the backbone were sulfated. Anticoagulant activity assays indicated that SCM exhibited strong anticoagulant activity by inhibiting intrinsic tenase (FXase) with IC 50 of 13.65 ng/mL, which may be a safer anticoagulant as an alternative to heparin-like drugs.
研究领域:
生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2021
·
IF 9.297
Wenqi Qin,Siqi Zhang,Shujuan Yang,Feilin Lin,Qichang Zhou,Houkui Gong,Xinyu Zhao,Furui He,Yang Zhou,Yuhong Feng,Gaobo Yu,Yuanyuan Liu,Jiacheng Li
Microalgae have been known as a promising sustainable resource for bioenergy cleaner production. Herein, a series of highly dispersible novel magnetic quaternary ammonium cationic surfactants (Fe 3 O 4 @DQn, n = 1, 2, 3) for the efficient harvesting of oleaginous Chlorella pyrenoidosa ( C. pyrenoidosa ) have been investigated. The Fe 3 O 4 @DQn capture microalgae process can be divided into a three-step operation (i.e., flocculation , separation, and recycle). For harvesting C. pyrenoidosa , the Fe 3 O 4 @DQn has both a high recovery efficiency (>98.4%) and a short flocculation time (2 min). In addition, the reused Fe 3 O 4 @DQ2 can maintain a high recovery efficiency (92.31%) with five cycling times. Moreover, the N -[3-(Trimethoxysilyl)propyl]ethylenediamine (TMPED) amount and the pH value both have a positive influence on the C. pyrenoidosa harvesting. It is worth noting that the dual effect of quaternary ammonium cationic surfactant, strong positively charged and hydrophobic properties, leads to a higher harvesting efficiency of microalgae. Furthermore, the results of particle size distribution and turbiscan stability index (TSI) show that a higher specific surface area, a smaller polydispersity index and TSI value of the Fe 3 O 4 @DQn can make a more excellent adsorption and dispersion properties. Finally, the Fe 3 O 4 @DQn might provide a promising high cost-performance to harvesting of oleaginous microalgae due to the better economic viability.
研究领域:
生物技术
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环境科学
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产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2023
·Vol. 20
·期 4
·3623
·
IF 4.614
Junrong Li,Yingjun Wang,Ziqi Fan,Panyang Tang,Mengting Wu,Hong Xiao,Zhenxing Zeng
Antibiotics have become a new kind of organic pollutant as they are widely used in the water environment of China. Tetracycline (TC) is a class of broad-spectrum antibiotics produced or semi-synthesized by actinomycetes. Metronidazole (MTZ) is the first generation of typical nitroimidazoles. The content of nitroimidazoles is relatively high in medical wastewater, and their ecotoxicity is worthy of attention because they are difficult to completely eliminate. In this paper, the effects of TC and MTZ on the growth, cell morphology, extracellular polymer and oxidative stress ofChlorella pyrenoidosa(C. pyrenoidosa) were studied, and the toxic interactions between TC and MTZ mixture components were analyzed. The results showed that the 96h-EC50of TC and MTZ was 8.72 mg/L and 45.125 mg/L, respectively. The toxicity of TC toC. pyrenoidosawas higher than that of MTZ, and the combined toxicity effect of TC and MTZ was synergistic after the combined action of a 1:1 toxicity ratio. In addition, the algal cells ofC. pyrenoidosadied to varying degrees, the membrane permeability of algal cells was increased, the membrane was damaged, the surface of algal cells exposed to higher concentration of pollutants was wrinkled, and their morphology was changed. The extracellular polymer ofC. pyrenoidosawas affected by a change in concentration. The effect of pollutants on the reactive oxygen species (ROS) level and malondialdehyde (MDA) content ofC. pyrenoidosaalso had an obvious dose–effect relationship. This study contributes to the assessment of the possible ecological risks to green algae due to the presence of TC and MTZ in aquatic environments.Keywords:tetracycline;metronidazole;Chlorella pyrenoidosa;single toxicity;combined toxicity
研究领域:
环境科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
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IF 4.5
Anqi Zhao,Xuejing Duan,Yang Guo,Zhuoqi Yu,Beixiao Zhang,Yongkun Lv,Alexei Solovchenko,Jifeng Chen,Jiangliang Xu
Chlorella based single cell protein (SCP) is a powerful candidate of sustainable protein source. The heterotrophic cultivation has been proved to be a more effective and industrially scalable route for the economical production of the Chlorella biomass, compare to traditional autotrophic cultivation. In this study, the heterotrophic cultivation medium and pulsed feeding strategy were optimized for the enhanced production of Chlorella pyrenoidosa CP2 based SCP. The content of total amino acids, biomass production, biomass productivity of C. pyrenoidosa CP2 respectively reached 50.64 %, 132.13 g/L and 18.87 g/L/d at 5-L fermenter scale. Moreover, the nutrient profile showed that the macronutrient contents, as well as the amino acid compositions and the essential fatty acid proportion of the heterotrophic C. pyrenoidosa CP2 biomass were comparable to those of the autotrophic biomass. The results indicated that the heterotrophic cultivation of C. pyrenoidosa CP2 has great potential for industrial production of microalgae based SCP.
研究领域:
微生物学
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生物工程
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工业生物技术
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应用与环境微生物学
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食品科学
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营养学
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DOI
高影响因子
危险品化学品经营许可证（带存储）