BIORESOURCE TECHNOLOGY
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2019
·
IF 6.669
Huan Li,Yun Zhang,Jiuyi Liu,Zhensheng Shen,An Li,Tian Ma,Qian Feng,Yingqiang Sun
The aim of this work was to study the biotreatment of mixed wastewaters collected from two points of MnO 2 industry by Chlorella vulgaris . Their growth rates in four mixed wastewaters with mass ratio of wastewater 1 # :2 # of 20:1, 50:1, 100:1, and 200:1 were characterized, and the lag phase was shortened with increase of nitrate concentrations. The N, P, and metal removal kinetics were quantified each other day to evaluate the bio-treatment efficiencies of high-nitrate wastewaters from MnO 2 industry. 84.68% and 98% of N, P has been removed. The Ca, Zn, Mn, and Si in mixed wastewaters was removed with maximum removal efficiencies of 97.91%, 99.37%, 99.44%, and 81.68%, respectively. The compositions of Chlorella vulgaris cultured in mixed wastewaters, including proteins, lipids, ash contents, and carbohydrates, were investigated in detail. The optimum HHV of Chlorella vulgaris about 18 MJ/Kg presented a potential to decrease the cost of algal biofuel.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Zhenyu Zhang,Li Wang,Yinqiang Wu,Chenshuo Li,Pengcheng Fu,Jing Liu
Microalgae , particularly Chlorella , have promising selenium (Se) enrichment capacity and great potential in wastewater treatment and the development of nutraceuticals. To acquire Chlorella strains with strong heterotrophic propensity and robust Se tolerance, this research utilized a heterotrophic isolation strategy along with a high Se concentration medium as selection factors. The combined sequence of the ribosomal small subunit (SSU) and internal transcribed spacer (ITS) was utilized for phylogenetic analysis , and comparative analysis of ITS secondary structures was also integrated to identify the desired strain. A novel subtropical microalgal strain, Chlorella vulgaris HNUFU001 with high Se tolerance, was isolated from water samples in a lake at Hainan University, China. Gaussian process regression was utilized to assess the strain's growth under varying Se concentrations. The intracellular accumulation and extracellular removal of Se were measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) and Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES), respectively. The maximum intracellular organic Se (92 % of total Se) was observed as 108.835 μg/g dry weight (DW) for 1 mg/L Se treatment. The highest Se removal rate was 40 %. The high Se tolerance and enrichment traits enable Chlorella vulgaris HNUFU001 to be a promising candidate for the heterotrophic production of organic Se-related nutraceuticals.
研究领域:
环境科学
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微生物学
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生物技术
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Yongrui Pi,Wenpeng Jia,Shengkai Chi,Hongke Meng,Yongzheng Tang
The Chlorella vulgaris - Rhodococcus erythropolis consortia was constructed for the biodegradation of waste motor oil (WMO), combined with thermodynamic calculations and stoichiometric analyses. The microalgae-bacteria consortium was constructed as C. vulgaris : R. erythropolis = 1:1 (biomass, cell/mL), pH = 7, 3 g/L WMO. Under the same condition, the terminal electron acceptors (TEAs) play a crucial role in the WMO biodegradation, which follows Fe 3+ >SO 4 2- > none. The biodegradation of WMO fitted well with the first-order kinetic model under experimental temperatures with different TEAs ( R 2 >0.98). The WMO biodegradation efficiency reached 99.2 % and 97.1 % with Fe 3+ and SO 4 2− as TEAs at 37 °C, respectively. Thermodynamic methanogenesis opportunity windows with Fe 3+ as TEA are 2.72 times fold as large as those with SO 4 2- . Microorganism metabolism equations demonstrated the viability of anabolism and catabolism on WMO. This work lays the groundwork for the implementation of WMO wastewater bioremediation and supports research into the biochemical process of WMO biotransformation .
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2016
·Vol. 23
·期 19
·19450-19460
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IF 2.76
Zhou Qiongzhi,Li Feng,Ge Fei,Liu Na,Kuang Yangduo
Toxicants are generally harmful to biotechnology in wastewater treatment. However, trace toxicant can induce microbial hormesis, but to date, it is still unknown how this phenomenon affects nutrient removal during municipal wastewater treatment process. Therefore, this study focused on the effects of hormesis induced by cetyltrimethyl ammonium bromide (CTAB), a representative quaternary ammonium cationic surfactant, on nutrient removal by Chlorella vulgaris F1068. Results showed that when the concentration of CTAB was less than 10 ng/L, the cellular components chlorophyll a , proteins, polysaccharides, and total lipids increased by 10.11, 58.17, 38.78, and 11.87 %, respectively, and some enzymes in nutrient metabolism of algal cells, such as glutamine synthetase (GS), acid phosphatase (ACP), H + -ATPase, and esterase, were also enhanced. As a result, the removal efficiencies of ammonia nitrogen (NH 4
+ ) and total phosphorus (TP) increased by 14.66 and 8.51 %, respectively, compared to the control during a 7-day test period. The underlying mechanism was mainly due to an enhanced photosynthetic activity of C. vulgaris F1068 indicated by the increase in chlorophyll fluorescence parameters (the value of Fv / Fm , ΦII, Fv / Fo , and rETR increased by 12.99, 7.56, 25.59, and 8.11 %, respectively) and adenylate energy charge (AEC) (from 0.68 to 0.72). These results suggest that hormesis induced by trace toxicants could enhance the nutrient removal, which would be further considered in the design of municipal wastewater treatment processes.
The schematic mechanism of C. vulgaris F1068 under CTAB induced hormesis. Green arrows ( ) represent the increase and the red arrow ( ) represents the decrease.
研究领域:
环境科学
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微生物学
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生物技术
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废水处理
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DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2021
·Vol. 107
·期 1
·147-153
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IF 2.151
Zhang Rui,Dong Pengyue,Sun Hongwen,Liu Chunguang
To investigate the combined effects of excess boron (B) and high salinity on the growth of freshwater algal species, Chlorella vulgaris and Microcystis aeruginosa were cultured in the medium with different B and salinities. The results show that high levels of B and salinity inhibited the growth of the two algal species. For C. vulgaris , low levels of B can alleviate the growth inhibition induced by salinity, and low levels of salinity can also relieve the growth inhibition induced by B. In contrast, high levels of salinity have little effect on B toxicity, while high levels of B aggravate salinity stress. For M. aeruginosa , salinity aggravates B toxicity, regardless of salinity levels. B supply worsens salinity stress on M. aeruginosa , regardless of supply doses. These results suggest that it may be possible to control algal bloom by regulating B or salinities.
研究领域:
生态学
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环境科学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2017
·
IF 5.651
Yang Peng,Aosong Deng,Xun Gong,Xiaomin Li,Yang Zhang
Considering the high concentration of mercury in industrial wastewater, such as coal-fired power plants and gold mining wastewater, this research study investigated the coupling process of lipid production and mercury bioremediation using microalgae cells. Chlorella vulgaris modified by biomimetic mineralization. The cultivation was divided in two stages: a natural cultivation for 7 days and 5 days of Hg 2+ addition (10–100 μg/L) for cultivation at different pH values (4–7) after inoculation. Next, the harvested cells were eluted, and lipid was extracted. The fluorescein diacetate (FDA) dye tests demonstrated that the mineralized layer enhanced the biological activity of microalgae cells in Hg 2+ contaminated media. Hg distribution tests showed that the Hg removal capacity of modified cells was increased from 62.85% to 94.74%, and 88.72% of eluted Hg 2+ concentration was observed in modified cells compared to 48.42% of raw cells, implying that more mercury was transferred from lipid and residuals into elutable forms.
研究领域:
环境生物学
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微生物学
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DOI
高影响因子
CHEMICAL ENGINEERING & TECHNOLOGY
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2013
·Vol. 36
·期 5
·733-739
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IF 1.366
C. Prommuak,P. Pavasant,A. T. Quitain,M. Goto,A. Shotipruk
Biodiesel and valuable free lutein were demonstrated to be simultaneously produced from Chlorella vulgaris lipid extracts. The alkali catalyst used in the transesterification of triglycerides acted as a reactant in converting lutein fatty acid esters to free lutein. A maximum biodiesel yield of 33.6 % by weight of the algal lipids was obtained after a 4-h reaction with MeOH at the MeOH/biomass ratio of 16:1 using 6 % alkali catalyst. The excess of alkali and MeOH employed in the production of biodiesel ensured the complete saponification of all lutein fatty acid esters to free lutein, giving a maximum yield of 2.3 % by weight of the algal lipids. In addition, a process for the separation of the biodiesel and free lutein products from the reaction mixture is proposed. Finally, a preliminary economic assessment was conducted, the results of which suggest that the process for the simultaneous production of biodiesel and lutein from C. vulgaris may be economically feasible.
研究领域:
生物工程
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能源生物学
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植物化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Qian Feng,Yabo Sun,An Li,Xiangang Lin,Tao Lu,Dan Ding,Menghan Shi,Yingqiang Sun,Yupeng Yuan
Booming graphitic carbon nitride (g-C 3 N 4 ) photocatalyzed water splitting increases crisis of aquatic contamination. However, a controversial understanding regarding effect of g-C 3 N 4 on growth of microalgae still exists. Accordingly, Chlorella vulgaris were cultured in 0–250 mg/L of g-C 3 N 4 with biomass named as C-0, C-50, C-100, C-150, C-200, and C-250, respectively. g-C 3 N 4 below 200 mg/L was beneficial to short-term cultivation of microalgae, while it was harmful to long-time cultivation. Protein factions of C-0, C-100, and C-250 were 41.4, 42.3, and 36.4 wt%, while their lipid factions varied from 21.5, 16.9, to 17.8 wt%, respectively. In short-term cultivation, superoxide dismutase’s activity of C-0, C-150, and C-250 increased dramatically, while accumulated H 2 O 2 led to increased activity of catalase . However, it started to decrease once antioxidant enzymes were per-oxidized, leading to increase of malondialdehyde content. In long-term cultivation, activities of superoxide dismutase, catalase and malondialdehyde content decreased dramatically owning to peroxidation of algae. Scavenger tests with tertiary butanol and triethanolamine implied that·OH was dominate parameter affecting growth of microalgae. This work indicates that g-C 3 N 4 below 200 mg/L is propitious to short-term cultivation of microalgae, while it is bad to long-time cultivation of microalgae, revealing dual rules of g-C 3 N 4 in Chlorella vulgaris cultivation.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2019
·
IF 2.87
Jiejun Xi,Jian Shao,Yuan Wang,Xiaoqin Wang,Hui Yang,Xinwei Zhang,Dongmei Xiong
Triflumizole is one of imidazole fungicides that works by inhibiting ergosterol biosynthesis , and is widely used for the control of powdery mildew and scabs on various fruits and crops. Triflumizole residue has been frequently detected in soil and aquatic ecosystems . While many studies have focused on its toxic effect on terrestrial and aquatic animals, little attention has been paid to aquatic algae, the primary producers of aquatic environments . Therefore, we evaluated the acute (96 h) toxicity effects of triflumizole on the freshwater algae Chlorella vulgaris , by examining growth, cell morphology, photosynthesis , and oxidative stress . The results showed that the 96 h median inhibition concentration (96 h-EC 50 ) was 0.82 mg / L (95% confidential interval 0.70–0.98 mg/L).The growth of algal cells was conspicuously inhibited by triflumizole exposure, and the cell surfaces appeared to be shrunkThe chlorophyll content (including Chl-a, Chl-b and T-Chl) dramatically decreased at triflumizole concentrations of 0.2 and 1.0 mg/L. In addition, the transcript abundance of photosynthesis-related genes ( psa B, psb C and rbc L) showed obvious decreases in above treatments after 96 h of exposure to triflumizole. Moreover, the algal growth inhibition was accompanied by an increase in intracellular reactive oxygen species and malondialdehyde content, as well as increased activity of antioxidant enzymes such as superoxide dismutase and peroxidase , indicating oxidative stress and lipid peroxidation . Our findings reveal that triflumizole has potential toxicity to the primary producers (freshwater algae) in aquatic ecosystems.
研究领域:
环境毒理学
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水生生态学
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植物学
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DOI
PubMed
高影响因子
BIOREMEDIATION JOURNAL
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2022
·
IF 2.14
Zhuo-yi Zhou,Yi-cheng Wu,Yi Kuang,Guan-zhu Lin,Hai-yan Fu,Ze-jie Wang
Ibuprofen, a most widely used anti-inflammatory drug, is frequently detectable in surface water, which might negatively affect the ecosystem. The present research investigated acute toxicity of ibuprofen to Chlorella vulgaris, and ibuprofen removal by C. vulgaris was also evaluated via a first order kinetic model. The results showed that ibuprofen in water has certain freshwater ecological risks, and ibuprofen-contaminated water can be effectively treated by C. vulgaris. The growth and the photosynthetic activity of C. vulgaris were decreased with the increase in ibuprofen exposure concentration, while superoxide dismutase and catalase activities were markedly promoted. The dry weight of C. vulgaris exposed to 100 mg/L ibuprofen was determined as 0.238 ± 0.008 g/L, which was 60.82% of the control group (without added ibuprofen in medium). The capacity of C. vulgaris to ibuprofen removal was decreased with the increase in ibuprofen concentration. The removal efficiency reached to 67.25% after 10-day treatment at ibuprofen concentration of 25 mg/L, while that was just 32.77% when the ibuprofen concentration increased to 100 mg/L. The ibuprofen removal by C. vulgaris accorded with the first-order kinetic model, and the removal rate constants of C. vulgaris to different ibuprofen concentrations (25, 50, 75 and 100 mg/L) were determined as 0.115, 0.097, 0.093 and 0.043 d−1, respectively.
研究领域:
环境科学
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水生毒理学
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生物修复
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Jirong Long,Wenguang Zhou,Jing Cao,Yuxuan Mao,Peilun Xu,Jin Liu,Yanghong Yao,Chenguang Wang,Yong Liu
Sporopollenin is a chemically robust biopolymer known for its resistance to environmental degradation and diverse functional potential. This study pioneers the successful extraction of sporopollenin from Chlorella vulgaris cultured in coal-fired flue gas using an innovative alkali-acid two-stage protocol. Multiscale characterization revealed that, despite its irregular morphology, microalgal sporopollenin shares high structural similarity with pollen-derived counterparts. Fourier-transform infrared spectroscopy and elemental analysis confirmed similar functional groups and elemental profiles. Liquid chromatography–mass spectrometry and pyrolysis–gas chromatography–mass spectrometry identified fatty acids as the major structural units, while solid-state 13 C SSNMR spectroscopy revealed a hybrid architecture comprising aliphatic, aromatic, and heterocyclic components. These findings provide molecular insight into the evolutionary continuity of sporopollenin and highlight a scalable, green approach for transforming industrial emissions into high-value biomaterials.
研究领域:
藻类学
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生物聚合物科学
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环境生物技术
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工业生物技术
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材料科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2024
·Vol. 2024
·期 1
·3828251
·
IF 3.5
Yan Wang,Xiao Kong,Mengdi Du,Yang Qiao,Minjie Ye,Bing Han,Jing Gan
Chlorella , a single-celled green algae rich in proteins, holds promise as a source of bioactive peptides. This research aimed to isolate an antioxidant peptide from Chlorella , examining its stability through digestion. Using Response Surface Methodology (RSM), we identified optimal hydrolysis conditions for Chlorella Protein Hydrolysate (CPH): 55°C, pH 8.75, and an enzyme-to-substrate ratio of 2.15%. These conditions led to CPH with notable radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (53.19 ± 0.99%), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (76.86 ± 0.28%), hydroxyl radical (53.39 ± 1.74%), and Fe 2+ chelating rates of 22.56 ± 2.97%. Subsequently, peptides under 3 kDa isolated by ultrafication proved particularly stable and antioxidative after in vitro digestion. In addition, further purify to obtain component C1 with DPPH radical scavenging ability of 59.62 ± 1.29% and an Fe 2+ chelating ability of 49.78 ± 1.55%. Subsequent LC-MS/MS identification and virtual screening led to the discovery of a stable and antioxidative peptide, Ser-Gly-His-His-Lys-Pro-Leu (SGHHKPL). The study provides the theoretical basis for the development and utilization of chlorella .
研究领域:
食品科学
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生物化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）