ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 19
·19450-19460
·
IF 2.76
Zhou Qiongzhi,Li Feng,Ge Fei,Liu Na,Kuang Yangduo
Toxicants are generally harmful to biotechnology in wastewater treatment. However, trace toxicant can induce microbial hormesis, but to date, it is still unknown how this phenomenon affects nutrient removal during municipal wastewater treatment process. Therefore, this study focused on the effects of hormesis induced by cetyltrimethyl ammonium bromide (CTAB), a representative quaternary ammonium cationic surfactant, on nutrient removal by Chlorella vulgaris F1068. Results showed that when the concentration of CTAB was less than 10 ng/L, the cellular components chlorophyll a , proteins, polysaccharides, and total lipids increased by 10.11, 58.17, 38.78, and 11.87 %, respectively, and some enzymes in nutrient metabolism of algal cells, such as glutamine synthetase (GS), acid phosphatase (ACP), H + -ATPase, and esterase, were also enhanced. As a result, the removal efficiencies of ammonia nitrogen (NH 4
+ ) and total phosphorus (TP) increased by 14.66 and 8.51 %, respectively, compared to the control during a 7-day test period. The underlying mechanism was mainly due to an enhanced photosynthetic activity of C. vulgaris F1068 indicated by the increase in chlorophyll fluorescence parameters (the value of Fv / Fm , ΦII, Fv / Fo , and rETR increased by 12.99, 7.56, 25.59, and 8.11 %, respectively) and adenylate energy charge (AEC) (from 0.68 to 0.72). These results suggest that hormesis induced by trace toxicants could enhance the nutrient removal, which would be further considered in the design of municipal wastewater treatment processes.
The schematic mechanism of C. vulgaris F1068 under CTAB induced hormesis. Green arrows ( ) represent the increase and the red arrow ( ) represents the decrease.
研究领域:
环境科学
·
微生物学
·
生物技术
·
废水处理
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Ruohua Qu,Qiting Xie,Jiang Tian,Min Zhou,Fei Ge
Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo -inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine , glutamine, alanine , and aspartic acid , as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.
研究领域:
环境科学
·
生物技术
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Na Liu,Han Zhang,Jinfeng Zhao,Yin Xu,Fei Ge
Microalgae photosynthesis is sensitive to coexisted contaminates in aquatic environment, thereby causes adverse effect on algal growth and nutrients uptake. Here, we investigated the photosynthetic toxicity mechanism of cetyltrimethyl ammonium chloride (CTAC)-induced on a green microalga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that CTAC reduced the algal growth rate, nutrients removal efficiency and weakened the photosynthetic performance. Meanwhile, the efficiency of oxygen evolution complex (OEC) and oxygen evolvement rates stressed by CTAC were significantly declined to 90.48% and 58.48% of the control (without CTAC), respectively. In addition, atomic force microscopy (AFM) detected the damage of PSII-OEC morphology and structure by CTAC. Furthermore, proteomic analysis showed that 41% of proteins were in the chloroplast thylakoid membranes which function in photosynthesis. The activity of oxygen-evolving enhancer protein 2 (OEE2 or PsbP) involved in electron transfer was significantly inhibited by CTAC, which down-regulated 15.14-fold in the presence of 0.6 mg/L CTAC. These results indicated that photosynthetic inhibition of CTAC mainly occurred in the PSII-OEC. This study provided a new perspective of the photosynthetic response in evaluation of environmental bioimpacts of surfactants on microalgae.
研究领域:
环境科学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）