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Genome-Wide Identification of the DnaJ Gene Family in Citrus and Functional Characterization of ClDJC24 in Response to Citrus Huanglongbing

Yuzhen Tian,Xizi Wang,Huoqing Huang,Xin Deng,Baihong Zhang,Yixuan Meng,Libo Wu,Hang Chen,Yun Zhong,Wenli Chen

Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide. The etiological agent responsible for this disease is “CandidatusLiberibacter asiaticus” (CLas), a phloem-restricted bacterium transmitted by psyllid vectors. To date, effective practical strategies for curing Citrus HLB remain elusive. Additionally, no susceptibility genes associated with HLB have been identified in Citrus species, thereby complicating the application of gene-editing techniques such as CRISPR-Cas9 to enhance resistance to HLB. The co-chaperoneDnaJplays a crucial role in protein folding and the regulation of various physiological activities, and it is also associated with multiple pathological processes.DnaJhas been extensively studied in many species, including Arabidopsis, rice, and wheat. However, there is limited information available regarding the DnaJ gene family in citrus. In this study, we conducted a comprehensive genome-wide analysis of the DnaJ family genes in various Citrus species. The Citrus genome was identified to contain 86DnaJgenes, which were unevenly distributed across nine chromosomes. Phylogenetic analysis indicated that these genes could be classified into six distinct groups. Furthermore, transcriptomic analysis revealed that nineDnaJgenes exhibited significantly higher induction in HLB-infected samples relative to non-HLB-infected Citrus.Cis-acting elements within the promoters ofDnaJgenes were also examined, revealing the presence of hormone and defense/stress responsiveness elements (TC-rich) distributed on theClDJC24gene. The results were validated using quantitative real-time PCR (qRT-PCR). Additionally, the silencing ofClDJC24suggested that this gene negatively regulates disease resistance in Citrus. Our study provided useful clues for further functional characterization and constructed a theoretical foundation for disease-resistant breeding in Citrus.