BIOLOGICAL CONTROL
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2020
·
IF 2.754
Zhiping Cai,Fang Ouyang,Jianwei Su,Xingrui Zhang,Chenglin Liu,Yunli Xiao,Jianping Zhang,Feng Ge
Plant volatiles mediate the interactions within plant-insect communities and influence the distribution and behavior of insects. Natural enemies often utilize the volatiles that released from plants to locate the profitable patches for foraging. The lady beetle Harmonia axyridis (Pallas) is a dominant generalist predator of various pests in agriculture fields, yet little is known about the chemical cues which the predator employs to locate its prey. In this study, we tested the attractiveness of volatiles emanating from healthy and aphid-infested insectary plant Cnidium monnieri (L.) Cuss. to H. axyridis adults in a Y-tube olfactometer. Next, using coupled gas chromatography – electroantennogram detection (GC-EAD) and gas chromatography – mass spectrometry (GC–MS) identified the volatile components which elicited electrophysiological activity in H. axyridis adults. Lastly, Y-tube olfactometer tests in laboratory and field traps were used to verify the effectiveness of the volatile components. In olfactometer trials, H. axyridis adults preferred healthy and aphid-infested plants versus control. In GC–MS and GC-EAD trials, two antenna-active components (1,2-diethylbenzene and p-diethylbenzene) were detected among the volatile blends emanating from healthy and aphid-infested plants; and in ensuing behavioral assays, H. axyridis adults were attracted to two synthetic volatile compounds. Under field conditions, the number of H. axyridis adults as recorded in traps for two synthetic volatile components at three different concentrations (100 mg/ml, 10 mg/ml, and 1 mg/ml) were significantly higher than those for mineral oil (control). These findings could provide an effective means for the cultivation of insectary plants to develop natural enemy attractants and support the biological control in the field.
研究领域:
昆虫学
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植物化学
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生物防治
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
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2021
·Vol. 18
·期 12
·e2100633
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IF 2.408
Ying-Hui He,Xiao-Fei Shang,Hai-Xin Li,An-Ping Li,Chen Tang,Bao-Qi Zhang,Zhi-Jun Zhang,Rui Wang,Yue Ma,Sha-Sha Du,Yong-Mei Hu,Tian-Lin Wu,Wen-Bin Zhao,Cheng-Jie Yang,Ying-Qian Liu
The increasing resistance of plant diseases caused by phytopathogenic fungi highlights the need for highly effective and environmentally benign agents. The antifungal activities of Cnidium monnieri fruit extracts and five isolated compounds as well as structurally related coumarins against five plant pathogenic fungi were evaluated. The acetone extract, which contained the highest amount of five coumarins, showed strongest antifungal activity. Among the coumarin compounds, we found that 4-methoxycoumarin exhibited stronger and broader antifungal activity against five phytopathogenic fungi, and was more potent than osthol. Especially, it could significantly inhibit the growth of Rhizoctonia solani mycelium with an EC 50 value of 21 μg mL −1 . Further studies showed that 4-methoxycoumarin affected the structure and function of peroxisomes, inhibited the β-oxidation of fatty acids, decreased the production of ATP and acetyl coenzyme A, and then accumulated ROS by damaging MMP and the mitochondrial function to cause the cell death of R. solani mycelia. 4-Methoxycoumarin presented antifungal efficacy in a concentration- dependent manner in vivo and could be used to prevent the potato black scurf. This study laid the foundation for the future development of 4-methoxycournamin as an alternative and friendly biofungicide.
研究领域:
植物病理学
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植物化学
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分子生物学
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杀菌剂
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PeerJ
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2020
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IF 2.379
Yuanyuan Shi,Shengxiang Zhang,Daiyin Peng,Chunmiao Shan,Liqiang Zhao,Bin Wang,Jiawen Wu
Cnidium monnieri (L.) Cuss ( C. monnieri ) is one of the most widely used traditional herbal medicines, exhibiting a wide range of pharmacological functions for treating asynodia, trichomonas vaginitis, and osphyalgia. Its important medicinal value comes from its abundance of coumarins. To identify genes involved in coumarin biosynthesis and accumulation, we analyzed transcriptome data from flower, leaf, root and stem tissues of C. monnieri . A total of 173,938 unigenes with a mean length of 1,272 bp, GC content of 38.79%, and N50 length of 2,121 bp were assembled using the Trinity program. Of these, 119,177 unigenes were annotated in public databases. We identified differentially expressed genes (DEGs) based on expression profile analysis. These DEGs exhibited higher expression levels in flower tissue than in leaf, stem or root tissues. We identified and analyzed numerous genes encoding enzymes involved in coumarin biosynthesis, and verified genes encoding key enzymes using quantitative real-time PCR. Our transcriptome data will make great contributions to research on C. monnieri and provide clues for identifying candidate genes involved in coumarin metabolic pathways.
研究领域:
植物学
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）