MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-7
·
IF 5.479
Zhang Limei,Qin Ju,Yang Qiang,Wei Siqi,Yang Rui
Carbon dots (CDs) were hydrothermally prepared from gelatin as the sole precursor. The CDs were hybridized with CoOOH nanosheets (NSs) to obtain a fluorescent probe (CD-CoOOH) for ascorbic acid (AA). The blue luminescence of the CDs, with excitation/emission maxima of 350/443 nm, is quenched by the NSs. If the NSs are reduced to Co 2+ by addition of AA, the CD-CoOOH hybrid is disintegrated and fluorescence recovers. The hybrid nanoprobe has a linear response in the 0.8–12.5 μM and 12.5–690 μM AA concentration ranges and a 0.025 μM limit of detection. Compared to previously reported methods for detecting AA, this methods has a wider linear range and a lower detection limit. The nanoprobe was utilized to quantify AA in spiked human serum samples and gave satisfactory results. The strategy was also applied to the construction of an “AND” logic gate, which avoided perplexed material decorations and chemical tracking.
Scheme 1. Schematic illustrations of the preparation of CDs and of the working principle for determination of ascorbic acid (AA).
研究领域:
分析化学
·
生物医学工程
·
材料科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 40
·4951-4957
·
IF 2.073
Zhi Yuan Mao,Lu Ning Zhu,Jie Gao,Jia Jun Liu,Yin Hui Wei,Xin Yu Li,Bin Cheng Yin,Jian Wang
As a major metabolic product of purine nucleotides, uric acid (UA) usually presents in urine and serum, which is closely related to human health. With the catalysis by uricase, uric acid can yield allantoin, CO2 and H2O2. Then, the produced H2O2 is able to decompose CoOOH nanoflakes into Co2+, resulting in a smaller size with weakened light scattering. This work provides a simple and selective light scattering method for UA analysis with a linear range from 1.0 × 10−5 to 1.0 × 10−3 M and a detection limit of 1.0 × 10−6 M. In addition, this method has been successfully applied to the determination of UA in human serum samples with similar results to clinical methods.
研究领域:
分析化学
·
医学诊断
·
生物传感
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产品关联
DOI
高影响因子
LUMINESCENCE
·
2021
·Vol. 36
·期 5
·1317-1326
·
IF 2.464
Lu Ning Zhu,Ru Cheng,Kai Wen Kang,Ming Yun Chen,Tianrong Zhan,Jian Wang
As a natural enzyme, alkaline phosphatase (ALP) plays an essential role in clinicopathological examinations and biomedical research, and is capable of hydrolyzing the phosphate group of l -ascorbic acid-2-phosphate (AAP) to yield l -ascorbic acid (L-AA). L-AA reduced cobalt oxyhydroxide (CoOOH) nanoflakes to Co 2+ , leading to a smaller size and weaker light scattering, which could be monitored by electron microscopic images and optical spectra. The indirect detection of ALP was achieved by the reduced light scattering signal of CoOOH nanoflakes. Under optimal conditions, the decrease in scattering intensity was proportional to the ALP concentration over the range 0.1–160 U/L and the detection limit was 0.034 U/L (3σ/ k ). Compared with other assays, this proposed light scattering method was more convenient and economic for ALP sensing. The method was successfully applied to ALP analysis in human serum samples, and was similar to the results obtained by commercial kits.
研究领域:
生物医学工程
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临床检验
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Ming Zhu,Jie Miao,Xiaoguang Duan,Daqin Guan,Yijun Zhong,Shaobin Wang,Wei Zhou,Zongping Shao
The deployment of an efficient catalyst is critical for successful application of peroxymonosulfate-based advanced oxidation processes to the rapid degradation of retardant organics in wastewater. Considering the rich properties of perovskite oxides and their drawbacks of low specific area and easy cation leaching, we reported a facile postsynthesis hydrothermal treatment method for preparing SrCo0.6Ti0.4O3−δ@CoOOH (SCT@CoOOH) nanocomposite as an efficient catalyst for PMS activation here. Surprisingly, CoOOH nanosheets were grown in situ over the surface of SCT substrate, resulting in a significantly increased surface area (22.1 m2 g–1), enhanced charge transfer capability, more generated surface oxygen defects and a strongly synergistic effect created between the bulk SCT and CoOOH surface layer. Remarkably, SCT@CoOOH exhibited higher (1.7 times) catalytic activity (0.84 mg L–1 min–1) for phenol degradation than SCT. Additionally, suppressed cobalt leaching was demonstrated in the SCT@CoOOH/PMS system. Notably, singlet oxygen as additional oxidative species were formed to accelerate phenol degradation in the radical-based SCT@CoOOH/PMS system due to the surface oxygen defects. The beneficial effect of higher pH value and different influence of foreign anions on the reation rate were also investigated. As a universal method, it may be also useful for the development of innovative functional materials for other various applications.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Minghui Xiong,Bo Chai,Guozhi Fan,Xiaohu Zhang,Chunlei Wang,Guangsen Song
The strong electron interaction between metal oxide–carbon-based catalyst components plays a vital role in the peroxymonosulfate (PMS) activation for pollutant degradation. Herein, a novel CoOOH nanosheets anchored on rape straw-derived biochar (BC) surface (labeled as CoOOH/BC) as an efficient PMS activator toward degrading sulfamethoxazole (SMX) was synthesized. Experimental results indicated that integrating CoOOH nanosheets on the BC surface could inhibit CoOOH aggregation to increase the specific surface areas, exert a component synergistic effect to enhance activation degradation activity, and improve the catalyst stability. As a result, a 96 % degradation efficiency of SMX was achieved within 20 min over 20 wt% CoOOH/BC composite catalyst under the optimal conditions. Density functional theory (DFT) calculations disclosed that a built-in electric field (BIEF) pointing from BC to CoOOH was constructed at their interface, which could mediate PMS activation for reactive oxygen species (ROS) generation and induce direct electron transfer from SMX to PMS, resulting in efficient SMX degradation via both radical and non-radical pathways. Moreover, quenching experiments and electron paramagnetic resonance (EPR) measurements confirmed that single oxide ( 1 O 2 ) and superoxide radical ( O 2 · - ) are the dominant active species in the current system. Additionally, the possible SMX degradation routes were reasonably proposed based on liquid chromatography-mass spectrometry (LC-MS) results. This work provides an in-depth understanding of the role of BIEF in PMS activation, and expands the application of biochar-based materials in the field of environmental remediation.
研究领域:
环境科学
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材料化学
·
污染修复
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DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yu-Lan Zhang,Cheng-Xiao Deng,Jian-Mei Zou,Jia-Le Hu,Wei Li,Jin-Fang Nie,Yun Zhang
Biothiol analysis holds critical importance in disease diagnosis, but the highly similar structures of biothiols pose a major obstacle to their practical detection. Herein, a straightforward yet efficient CoOOH nanoflake (CoOOH NF)-triggered dual-color fluorescence sensor array has been developed for the discrimination of biothiols. Owing to their high oxidase-like activity, CoOOH NFs efficiently catalyze the oxidation of fluorometric signal indicators, generating diverse fluorescence signals. However, the presence of biothiols induced structural degradation of CoOOH NFs via redox etching, thereby inhibiting the fluorescence reactions. Based on the differential reducing capacities of biothiols, the sensor array produced unique fluorescence response patterns, which were further analyzed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). The results demonstrated that the sensor array could reliably discriminate three biothiols (glutathione (GSH), cysteine (Cys), and homocysteine (Hcy)) across a broad concentration range (0.5–100 μM), as well as their mixtures with varying molar ratios. Importantly, this strategy was successfully applied to distinguish cancer cells from normal cells and to analyze clinical serum samples, highlighting its potential for medical diagnostics.
研究领域:
分析化学
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生物传感
·
医学诊断
·
纳米材料
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 42
·5081-5085
·
IF 2.596
Hongqi Chen,Huihui Wang,Wanying Xia,Jianguo Zhang,Lun Wang
In this work, we developed a simple and selective luminescence sensing system for detecting ascorbic acid (AA) based on NaGdF4:Yb,Er@NaYF4 upconversion nanoparticles (UCNPs) and oxidase-like CoOOH nanoflakes. When p-phenylenediamine (PPD) and oxidase-like CoOOH nanoflakes were added to the UCNPs solutions, PPD, as a typical substrate of oxidase, could be oxidized to PPDox. The luminescence intensity of UCNPs was quenched because PPDox could play a role as an energy acceptor. When AA was added to the above solution mixture, the CoOOH nanoflakes were destroyed, and then, the luminescence intensity was recovered. Under the best experimental conditions, the linear range for detection of AA was 0.8 to 280 μM, and the detection limit was 0.25 μM. Furthermore, the system could be applied to real sample analysis with satisfactory results.
研究领域:
分析化学
·
纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Yanping He,Peixuan Li,Xin Zhao,Dongmei Wang,Fuqiang Zhang
The abuse or misuse of fleroxacin (FLE) in aquaculture and livestock may elevate FLE residues in foods, posing a serious threat to human health. Consequently, FLE detection has garnered extensive attention in food science. This study developed a magnetic separation-assisted colorimetric aptasensor for ultrasensitive FLE detection, leveraging the enhanced nanozyme activity of CoOOH nanoflakes with hairpins and dual cascade amplification (CHA-HCR). Under optimal experimental conditions, the proposed aptasensor exhibited high specificity for FLE with a wide linear range (1–100 nM) and a detection limit as low as 758 pM. Furthermore, the colorimetric aptasensor demonstrated robust performance in FLE-spiked honey analysis, achieving 96.70–101.06 % recoveries with 2.42–5.45 % RSDs, which showed strong agreement with commercial ELISA results. This study establishes a simple and effective platform for sensitive FLE detection in honey samples, demonstrating significant potential for practical food safety monitoring.
研究领域:
食品科学
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分析化学
·
纳米技术
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环境卫生
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生物传感器
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产品关联
DOI
高影响因子
Small
·
2024
·
IF 13
Yingyan Ma,Yuan Ha,Liangqiang Chen,Ziqi An,Linzhuang Xing,Zhenni Wang,Zhimin Li
Efficient and affordable price bifunctional electrocatalysts based on transition metal oxides for oxygen and hydrogen evolution reactions have a balanced efficiency, but it remains a significant challenge to control their activity and durability. Herein, a trace Ru (0.74 wt.%) decorated ultrathin CoOOH nanosheets (≈4 nm) supported on the surface of nickel foam (Ru/CoOOH@NF) is rationally designed via an electrochemically induced strategy to effectively drive the electrolysis of alkaline overall water splitting. The as-synthesized Ru/CoOOH@NF electrocatalysts integrate the advantages of a large number of different HER (Ru nanoclusters) and OER (CoOOH nanosheets) active sites as well as strong in-suit structure stability, thereby exhibiting exceptional catalytic activity. In particular, the ultra-low overpotential of the HER (36 mV) and the OER (264 mV) are implemented to achieve 10 mA cm −2 . Experimental and theoretical calculations also reveal that Ru/CoOOH@NF possesses high intrinsic conductivity, which facilitates electron release from H 2 O and H-OH bond breakage and accelerates electron/mass transfer by regulating the charge distribution. This work provides a new avenue for the rational design of low-cost and high-activity bifunctional electrocatalysts for large-scale water-splitting technology and expects to help contribute to the creation of various hybrid electrocatalysts.
研究领域:
电化学
·
材料科学
·
催化
·
能源转换与存储
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
Kong Weisu,Tan Qingqing,Guo Haiyu,Sun Han,Qin Xia,Qu Fengli
A method is described for photoelectrochemical determination of the activity of alkaline phosphatase (ALP). It employs an indium tin oxide (ITO) electrode modified a CdS quantum dots@graphene (CdS@GR) composite and hexagonal cobalt oxyhydroxide (CoOOH) nanosheets. The CdS@GR nanocomposite was synthesized by assembling the CdS quantum dots onto a GO film to receive a basic photocurrent response of the ITO. This is further improved by covering it with CoOOH nanosheets. Secondly, 2-phospho-L-ascorbic acid (AAP) is added as a substrate for ALP. Its hydrolysis yields ascorbic acid which reduces CoOOH to form cobalt(II) ion. As a result, the CoOOH nanosheets decompose. This is accompanied by a reduction of the photocurrent. The effect was used to design a selective and sensitive assay of determination of the activity of ALP. Under the optimized experimental conditions, response is linear in the 10 to 300 U·L −1 ALP activity range. The detection limit is 1.5 U·L −1 at a signal-to-noise ratio of 3.
Indium tin oxide (ITO) was coted with CdS@graphene and CoOOH to obtain a material with superior photoelectrochemical properties. The detection of alkaline phosphatase (ALP) was accomplished by using 2-phospho-L-ascorbic acid (AAP) which is hydrolyzed by ALP to release ascorbic acid (AA) which reduces CoOOH to Co 2+ .
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-8
·
IF 5.479
Zhou Jiao,Ling Yu,Li Nian Bing,Luo Hong Qun
The authors describe a fluorometric and resonance Rayleigh scattering dual-mode scheme for detection of the activity and inhibition of alkaline phosphatase (ALP). The method utilizes (a) CoOOH nanoflakes, which have high resonance Rayleigh scattering activity and can strongly adsorb ssDNA, and (b) Co(II)-dependent DNAzyme assisted signal amplification. ALP specifically catalyzes the hydrolysis of ascorbic acid-2-phosphate to produce ascorbic acid which reduces CoOOH nanoflakes to Co(II) ion. The Co(II)-dependent DNAzyme is then activated by Co(II) ion, and this results in the cleavage of a substrate labeled with both a fluorophore and a quencher. Following hydrolysis, fluorophore and quencher become separated and the fluorescence measured at excitation/emission wavelengths of 490/518 nm recovers, while the RRS signal at 405 nm decreases. The method works on the 0.2 to 2000 U L −1 ALP activity range, and the detection limit is 0.05 U L −1 . The method was used to validate the mechanism of the action of two classical ALP inhibitors (EDTA and Na 3 VO 4 ). Conceivably, it can also be applied to screen for ALP inhibitors.
A bioprobe composed of cobalt oxyhydroxide (CoOOH) nanoflakes as the substrate and carrier, and Co 2+ -dependent DNAzyme containing guanine ribonucleotide (rG) and uracil ribonucleotide (rU) for circularly signal amplification is used for fluorometric determination of alkaline phosphatase (ALP) activity.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 18
·2843-2850
·
IF 4.776
Shi Gang Liu,Lei Han,Na Li,Na Xiao,Yan Jun Ju,Nian Bing Li,Hong Qun Luo
Nanozymes are increasingly exploited as components in bioanalysis and diagnostics. Here, we report a fluorescence and colorimetric dual-mode assay for alkaline phosphatase (ALP) activity employing oxidase-like cobalt oxyhydroxide (CoOOH) nanoflakes. Colorless o-phenylenediamine (OPD), a substrate for oxidase, can be oxidized to a product (OxOPD) with yellow color and orange fluorescence in a CoOOH nanoflake solution. But, ascorbic acid (AA) is able to reduce CoOOH to cobalt ion (Co2+), which causes the decomposition and collapse of the CoOOH nanoflakes, and thereby the CoOOH nanoflakes are deprived of the oxidase-like property. Based on this principle, the fluorescence and colorimetric dual-mode detection of AA was achieved. Alkaline phosphatase (ALP) can make L-ascorbic acid-2-phosphate (AAP) hydrolyze to yield AA. As a result, with the help of AAP, the selective and sensitive dual-mode assay of ALP activity has been realized successfully by using fluorescence and UV-vis absorption spectroscopies. Quantitative analysis of ALP in human serum samples and an ALP inhibitor investigation were performed using this sensing system. Given the economical and sensitive properties, the proposed method based on CoOOH nanoflakes has great potential for not only probing ALP activity in biological systems but also screening potential ALP inhibitors. Meanwhile, in this study, a new insight has been provided into the application of CoOOH nanoflakes in the development of sensitive and convenient sensors.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）