Zearalenone (ZEN) poses significant threats to food safety and human health due to its widespread contamination in cereals. Current detection methods face limitations in cost, complexity, and field applicability. To address this, we developed a novel colorimetric aptasensor leveraging the peroxidase-like activity of Au/CoOOH nanozymes for rapid and sensitive ZEN detection. Systematic characterization of cobalt-based nanomaterials (β-Co(OH) 2 , Co 3 O 4 , CoOOH, and Au/CoOOH) revealed that Au/CoOOH exhibited the highest catalytic efficiency, achieving a 3.5-fold enhancement over CoOOH due to synergistic interactions with Au nanoparticles. The aptasensor employed a competitive displacement strategy: ZEN binding to aptamer-functionalized magnetic beads released cDNA-conjugated Au/CoOOH, reducing 3,3′,5,5′-tetramethylbenzidine oxidation signals. Optimized parameters enabled a linear detection range of 0.3–500 ng/mL with a detection limit of 0.23 ng/mL. Specificity tests confirmed minimal cross-reactivity with other mycotoxins. The sensor demonstrated exceptional sensitivity, specificity, and practicality, validated by recovery rates of 90.65–105.63 % in spiked matrices, positioning it as a reliable tool for safeguarding food safety through rapid, precise mycotoxin monitoring.
Yanyun Zou,Jinyang Chen,Qingli Chai,Ting Zhu,Shasha Zeng,Yucheng Liu
As a class of intelligent materials, stimuli-responsive hydrogels enable attractive sensors for sensitive and selective detection along with the potential for low production costs. Herein, we proposed a stimuli-responsive colorimetric sensor based on Cu 2+ -crosslinked alginate hydrogel loaded with CoOOH nanozyme. Upon binding of Cu 2+ to pyrophosphate ion (PPi), the hydrogel would be disassembled by PPi, which controlled the release of CoOOH nanoflakes . Interestingly and importantly, it was found that PPi and PPi-Cu 2+ could further enhance the catalytic activity of the released CoOOH nanozyme. Since alkaline phosphatase (ALP) can catalyze the hydrolysis of PPi to phosphate ion (Pi), a sensitive colorimetric sensor for ALP detection was constructed based on PPi-triggered controlled release and enhancing activity of CoOOH nanozyme. The as-constructed sensor exhibited good linear response to ALP over the range from 0.1 to 250 mU/mL with a detection limit of 0.07 mU/mL. Additionally, this sensor was also extended to measure two typical ALP inhibitors (Na 3 VO 4 and EDTA) with reasonable results. Last but not least, it was confirmed that the detection results of ALP in real serum samples with the proposed sensor were in accordance with the data from clinical test, which indicates the robustness and practicability of this stimuli-responsive colorimetric sensor.
危险品化学品经营许可证（带存储）