LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Qi Sun,Miaomiao Ma,Zhijuan Li,Xiang Chen,Jian Zeng,Meiqi Huang,Yuting Yin,Xueyan Ran,Jianmei Li,Tao Le
Zearalenone (ZEN) poses significant threats to food safety and human health due to its widespread contamination in cereals. Current detection methods face limitations in cost, complexity, and field applicability. To address this, we developed a novel colorimetric aptasensor leveraging the peroxidase-like activity of Au/CoOOH nanozymes for rapid and sensitive ZEN detection. Systematic characterization of cobalt-based nanomaterials (β-Co(OH) 2 , Co 3 O 4 , CoOOH, and Au/CoOOH) revealed that Au/CoOOH exhibited the highest catalytic efficiency, achieving a 3.5-fold enhancement over CoOOH due to synergistic interactions with Au nanoparticles. The aptasensor employed a competitive displacement strategy: ZEN binding to aptamer-functionalized magnetic beads released cDNA-conjugated Au/CoOOH, reducing 3,3′,5,5′-tetramethylbenzidine oxidation signals. Optimized parameters enabled a linear detection range of 0.3–500 ng/mL with a detection limit of 0.23 ng/mL. Specificity tests confirmed minimal cross-reactivity with other mycotoxins. The sensor demonstrated exceptional sensitivity, specificity, and practicality, validated by recovery rates of 90.65–105.63 % in spiked matrices, positioning it as a reliable tool for safeguarding food safety through rapid, precise mycotoxin monitoring.
研究领域:
食品安全
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Kongxiang Wei,Honghong Rao,Xin Xue,Mingyue Luo,Zhonghua Xue
Nanozymes-catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) reaction systems have been extensively used for fabricating colorimetric sensors, but they still require advanced instruments like color analysis software or ultraviolet spectrophotometers to achieve accurate quantitative analysis. Herein, we explored an alternative strategy to convert a color readout to a temperature readout of a CoOOH nanoflakes-TMB colorimetric reaction system, and successfully fabricated a simple and facile photothermometric sensor of acid phosphatase (ACP) by using a common thermometer as the reader. It mainly performed based on the strong photothermal effect of the oxidation product (TMBox) of TMB upon an 808 nm near-infrared (NIR) laser irradiation, which showed a close relationship with the ACP content with a lower limit of detection (LOD) of 0.037 mU/mL for photothermometric ACP determination. This photothermometric method was proved to be suitable for not only sensing ACP but also screening its inhibitor in human serum, and providing a simple, rapid, convenient, low cost, and portable organic chromogenic substrate-based sensing platform.
研究领域:
分析化学
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生物传感器
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yanyun Zou,Jinyang Chen,Qingli Chai,Ting Zhu,Shasha Zeng,Yucheng Liu
As a class of intelligent materials, stimuli-responsive hydrogels enable attractive sensors for sensitive and selective detection along with the potential for low production costs. Herein, we proposed a stimuli-responsive colorimetric sensor based on Cu 2+ -crosslinked alginate hydrogel loaded with CoOOH nanozyme. Upon binding of Cu 2+ to pyrophosphate ion (PPi), the hydrogel would be disassembled by PPi, which controlled the release of CoOOH nanoflakes . Interestingly and importantly, it was found that PPi and PPi-Cu 2+ could further enhance the catalytic activity of the released CoOOH nanozyme. Since alkaline phosphatase (ALP) can catalyze the hydrolysis of PPi to phosphate ion (Pi), a sensitive colorimetric sensor for ALP detection was constructed based on PPi-triggered controlled release and enhancing activity of CoOOH nanozyme. The as-constructed sensor exhibited good linear response to ALP over the range from 0.1 to 250 mU/mL with a detection limit of 0.07 mU/mL. Additionally, this sensor was also extended to measure two typical ALP inhibitors (Na 3 VO 4 and EDTA) with reasonable results. Last but not least, it was confirmed that the detection results of ALP in real serum samples with the proposed sensor were in accordance with the data from clinical test, which indicates the robustness and practicability of this stimuli-responsive colorimetric sensor.
研究领域:
材料科学
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分析化学
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生物传感器
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 9
·2991-3003
·
IF 4.478
Rao Honghong,Li Jianying,Luo Mingyue,Zhang Kehui,Gou Hao,Liu Haixia,Xue Zhonghua
Ratiometric electrochemical assays have been demonstrated to be more sensitive and selective in various sensing events, mainly due to their affordable built-in correction and good self-reference capability. But it is known that complicated modification and labeling operations usually are necessary for the construction of ratiometric electrochemical assays, therefore is a hot and important issue needing consideration carefully. We herein report a new yet simple bare electrode-based ratiometric electrochemical bioassay to achieve sensitive and selective analysis of alkaline phosphatase (ALP), using a liquid phase system that contains CoOOH nanozymes and commercially available indicator substrate. This proposed bioassay works based on the ratiometric change of dual electrochemical signals, arising from an exclusive target ALP-triggered hydrolysis of electrochemical substrate p-nitrophenyl phosphate (PNPP). In this design, the two hydrolyzed products of electrochemically active p-nitrophenol (PNP) and electrochemically inactive phosphate anion (PO 4 3− ) are responsible together for the ratiometric electrochemical analysis of ALP. PNP exhibits a straightforward current response toward ALP content; however, PO 4 3− cannot show a direct electrochemical signal thus is rationally designed to offer an alternative response by linking it with the specific CoOOH nanozyme-catalyzed reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 , in which the nanozyme-catalyzed product oxTMB shows a direct reduction current at the GCE, and significantly decreases with increasing PO 4 3− species due to the good inhibition of PO 4 3− toward CoOOH nanozyme activity. As a result, a ratiometric electrochemical strategy for ALP analysis with a low limit of detection of 0.366 U/L (S/N = 3) was successfully achieved by integrating the above direct and indirect dual electrochemical responses. This developed bioassay can allow the quantitative diagnosis of ALP activity especially with a label-free and modification-free merit, therefore paving the way for simple, convenient, and portable electroanalytical tools in biosensing design and application. Graphical abstract
研究领域:
分析化学
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生物化学
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电化学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）