Commelina communis L., a functional food and herbal plant in Asia, has been used against obesity, diabetes, and infections for centuries. A growing body of studies has demonstrated that indigestible polysaccharides are significant in obesity management. However, the structures and bioactivities of homogeneous polysaccharides from C. communis remain unclear. This study presented the structural characterization, simulated digestion, and human gut Bacteroides proliferation promotion activity of a novel homogeneous polysaccharide (CCB-3) from C. communis . The results showed that CCB-3 was an arabinoglucuronoxylan, primarily composed of arabinose, galactose, xylose, glucuronic acid (GlcA), and 4- O -methyl GlcA with a molecular weight ( Mw ) of 58.8 kDa. Following a 6-hour exposure to simulated gastrointestinal fluid, the Mw of CCB-3 remained unchanged, revealing that CCB-3 was an indigestible polysaccharide. Notably, CCB-3 could promote the proliferation of B. thetaiotaomicron, B . ovatus , and B . cellulosilyticus and produce short-chain fatty acids (SCFAs) and 1,2-propanediol. These findings might shed light on the discovery of polysaccharide-based leading compounds from C. communis against obesity.
Keda Ying,Xinru Ren,Meiru Tong,Siyu Ye,Yuhong Shi,Qianjin Zhou,Jiong Chen
Multidrug-resistant (MDR) pathogens are a significant threat to aquaculture due to their resistance to most conventional drugs. AcrAB-TolC is a known MDR efflux pump that increases antibiotic resistance. Inhibiting such pumps is a promising strategy in drug discovery. In this study, phytocompounds derived from Commelina communis (Asiatic dayflower) have been identified as candidate inhibitors for AcrB of Aeromonas hydrophila through virtual screening, encompassing molecular docking and molecular dynamics (MD) simulation. Consequently, five phytocompounds from the Asiatic dayflower met the criteria as suggested by the TCMSP database. Among them, flavocommelin, luteolin, and delphin_qt, exhibiting values higher than or equal to the control PAβN, emerged as candidate inhibitors of AcrB protein according to molecular docking analysis. Luteolin, which adhered to Lipinski's rule of five, was stable in binding to AcrB in a 50 ns molecular dynamics simulation. Luteolin also attenuated the reduction in Nile Red fluorescence in a dose-dependent manner, indicating the actual inhibition of the AcrAB-TolC efflux pump of the MDR A. hydrophila Y-1. The checkerboard synergy analysis revealed that luteolin exerted a positive impact on the six tested antibiotics, with fractional inhibitory concentration (FIC) indexes ranging from 0.125 to 0.375. Luteolin and florfenicol were recognized for having the best synergistic activity. When Ctenopharyngodon idella (grass carp) and Plecoglossus altivelis (ayu) were infected by A. hydrophila Y-1, the combination of luteolin and florfenicol markedly enhanced the survival rate and declined the bacterial load, as compared to either luteolin or florfenicol alone. Thus, luteolin is an effective efflux pump inhibitor for AcrAB-TolC of A. hydrophila , enhancing antibiotic sensitivity and exhibiting synergistic effects with antibiotics.
危险品化学品经营许可证（带存储）