This work investigated the quenching effect of ferrocenecarboxylic acid (Fc-COOH) on the bovine serum albumin-stabilized Au nanoclusters-triethylamine (Au 25 -Et 3 N) electrochemiluminescence (ECL) system. Based on the annihilation between the oxidation product of Fc (Fc + ) and Et 3 N radical, a quenching-based sandwich-configuration ECL biosensor was established for sensitive and specific detection of concanavalin A (ConA). Au 25 with strong initial ECL signal were modified onto the glassy carbon electrode (GCE) for immobilizing glucose oxidase (GOD). With the further immobilization of target ConA, the quenching probe of GOD@Fc-COOH-PtPd nanocubes were incubated onto the electrode through a specific carbohydrate-ConA interaction, thus achieving a sandwich structure. The resulting ECL biosensor showed a wide linear range from 0.004 ng/mL to 90 ng/mL and a low detection limit of 0.001 ng/mL (S/N = 3) for ConA detection. The integration of Fc-COOH with Au 25 -Et 3 N system would hold a great promise in bioanalysis.
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
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