INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Jie Zhou,Huili Ma,Min Guan,Junfen Feng,Xiaomeng Dong,Yuxin Wei,Tong Zhang
The smart oral administration Insulin device has the potential to improve glycemic management. It can reduce the risk of hypoglycemia associated with exogenous Insulin (INS) therapy while also avoiding many of the disadvantages associated with subcutaneous injections. Furthermore, diabetes mellitus (DM) is an endocrine illness characterized by inflammation, and it is critical to minimize the amount of inflammatory markers in diabetic patients while maintaining average blood glucose. In this study, a responsive nanosystem vitamin B12-Fucoidan-Concanavalin A (VB12-FU-ConA NPs) with anti-inflammatory action was developed for smart oral delivery of Insulin. Con A has high sensitivity and strong specificity as a glucose-responsive material. Fucoidan has anti-inflammatory, immunomodulatory, and hypoglycemic functions, and it can bind to Con A to form a reversible complex. Under high glucose conditions, free glucose competitively binds to Con A, which swells the nanocarrier and promotes Insulin release. Furthermore, in the low pH environment of the gastrointestinal tract, positively charged VB12 and anionic fucoidan bind tightly to protect the Insulin wrapped in the carrier, and VB12 can also bind to intestinal epithelial factors to improve transit rate, thereby promoting INS absorption. In vitro tests showed that the release of nanoparticles in hyperglycemic solutions was significantly higher than the drug release in normoglycemic conditions. Oral delivery of the nanosystems dramatically lowered blood glucose levels in type I diabetic mice (T1DM) during in vivo pharmacodynamics, minimizing the risk of hypoglycemia. Blood glucose levels reached a minimum of 8.1 ± 0.4 mmol/L after 8 h. Administering the nanosystem orally notably decreased the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in diabetic mice. The nano delivery system can be degraded and metabolized in the intestinal tract after being taken orally, demonstrating good biodegradability and biosafety. In conclusion, the present study showed that VB12-FU-ConA nanocarriers are expected to be a novel system for rationalizing blood glucose.
研究领域:
药学
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纳米技术
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糖尿病研究
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药物递送系统
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生物医学工程
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PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Sijie Yuan,Ying Tu,Ru Yu,Fei Nie
Introducing novel catalysts is essential for developing chemiluminescence (CL) systems that exhibit sustained and robust emission. Traditional Luminol-H2O2 systems typically feature flash-type CL emission. In this study, we discovered that the porous material HKUST-1 can induce a long-lasting and intense CL emission when combined with Luminol-H2O2. This long-term emission signal can be directly detected by the smartphone. By changing the calcination temperature, a series of microporous and hierarchically porous HKUST-1 materials were prepared as catalysts to adjust the kinetic characteristics of the CL signal of Luminol-H2O2 system from flash-type to glow-type. A systematic investigation into the influence of the central metal and ligand, aperture, and particle size of HKUST-1 on the CL kinetic properties revealed that the pore structure has the most pronounced impact on the dynamics of the Luminol-H2O2 CL reaction. Capitalizing on the intense emission of the HKUST-1-catalyzed Luminol-H2O2 system, we established a CL sandwich immunoassay strategy for concanavalin A (ConA), demonstrating good linearity and low detection limit. This research presents a significant endeavor in modulating the dynamics of CL signal emissions.
研究领域:
分析化学
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生物传感
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材料科学
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PubMed
高影响因子
RSC Advances
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2015
·Vol. 5
·期 73
·59603-59608
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IF 3.84
Shuguang Yan,Yurong Tang,Mengling Yu
Herein we developed a highly sensitive methodology for heparin detection based on a resonance Rayleigh scattering (RRS) spectral assay. Heparin and concanavalin A (conA) densely stacked together and formed nanoparticles or a well-defined brick-and-mortar nanostructure. A strong electrostatic attraction, hydrogen bond and the solid–liquid interface induced RRS enhancement. The result of absorption spectra and TEM testified that the introduction of heparin indeed changed the structure of conA and formed brick-and-mortar nanostructures. The optimum reaction conditions of the method and the reasons for RRS enhancement are also discussed. Combined with an RRS spectral assay, an effective biosensor has been developed for heparin. The bioassay allows sensitive detection of heparin with a detection limit of 2.48 ng mL−1 (3σ), a linear range from 8.28 ng mL−1 to 2.5 μg mL−1. The method was successfully applied to the determination of heparin in heparin sodium injection samples.
研究领域:
分析化学
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生物医学工程
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药学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Min Cai,Congyu Wu,Muqing Wang,Qian Chen,Yilin Guo,Yu Liu,Xin Huang,Yuan Gao,Yun Qi
Ethnopharmacological relevance Elephantopus scaber L. ( E. scaber ) is a heat-clearing and detoxifying traditional Chinese medicine. Clinically, it is used for treating some immuno-inflammatory diseases associated with excessive T cell activation. However, its action and mechanism on T cells are still ambiguous. Aim of the study This study aims to investigate the molecular mechanism of the ethanol extract of E.scaber (ESE) in ConA-induced T cell activation models. Materials and methods IL-2 transcription and translation levels were determined by RT- q PCR and ELISA. CD4 + naïve T cells were sorted using the flow cytometer. Western blot assay was used for determining intracellular protein levels. Cytoplasmic and mitochondrial Ca 2+ concentrations were measured by fluorometric assays. Results ESE not only dampened the proliferation of splenic lymphocytes, but also decreased IL-2 production in ConA-activated T cells without affecting its mRNA stability. It could inhibit the activation of NFAT and NF-κB pathways, showed as impeding the nuclear translocation of NFAT and p65. Regarding the upstream signaling, ESE significantly decreased cytoplasmic Ca 2+ (Ca 2+ [c] ) level in a model-independent manner. Although it failed to affect plasma membrane Ca 2+ -ATPase, Na + /Ca 2+ exchanger, and mitochondrial Ca 2+ uptake, it affected sarco/endoplasmic Ca 2+ -ATPase (SERCA) pathway. It also hindered IκBα phosphorylation and subsequent degradation. In vivo , ESE reduced IL-2 levels in local and systemic T cell activation models. Conclusion Through inhibiting the activation of SERCA/Ca 2+ [c] /NFAT and NF-κB pathways, ESE markedly decreased ConA-induced IL-2 production in vitro and in vivo . This study provides not only a modern explanation for the traditional uses of E. scaber , but also a scientific guidance for clinical practice.
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PubMed
高影响因子
ELECTROCHIMICA ACTA
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2017
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IF 4.798
Shihong Chen,Yu Fan,Cong Zhang,Yanyan He,Shaping Wei
This work investigated the quenching effect of ferrocenecarboxylic acid (Fc-COOH) on the bovine serum albumin-stabilized Au nanoclusters-triethylamine (Au 25 -Et 3 N) electrochemiluminescence (ECL) system. Based on the annihilation between the oxidation product of Fc (Fc + ) and Et 3 N radical, a quenching-based sandwich-configuration ECL biosensor was established for sensitive and specific detection of concanavalin A (ConA). Au 25 with strong initial ECL signal were modified onto the glassy carbon electrode (GCE) for immobilizing glucose oxidase (GOD). With the further immobilization of target ConA, the quenching probe of GOD@Fc-COOH-PtPd nanocubes were incubated onto the electrode through a specific carbohydrate-ConA interaction, thus achieving a sandwich structure. The resulting ECL biosensor showed a wide linear range from 0.004 ng/mL to 90 ng/mL and a low detection limit of 0.001 ng/mL (S/N = 3) for ConA detection. The integration of Fc-COOH with Au 25 -Et 3 N system would hold a great promise in bioanalysis.
研究领域:
生物分析
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电化学
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DOI
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 9
·1-11
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IF 6.232
Tang Tingting,Yang Fan,Wang Lina,Zhao Chunxin,Nie Fei,GuopingYang
An ultrasensitive sandwich electrochemiluminescence (ECL) biosensor was designed for determination of concanavalin A (ConA) through the specific carbohydrate-ConA interactions. Three-dimensional porous metal-organic framework (Zn-MOF) was synthesized, which loaded a large amount of luminescent reagents as luminol by encapsulating into its pores to form Zn-MOF@luminol complex. Interestingly, Zn-MOF also acted as the coreactant accelerator in the luminol-H 2 O 2 ECL system. This Zn-MOF@luminol complex was used as the signal probe to achieve a super strong and stable ECL signal. In addition, three-dimensional hierarchical molybdenum disulfide nanoflower and multiwalled carbon nanotubes complex (MoS 2 NF@MWCNTs) with peroxidase-mimicking enzyme property were used as a substrate to modify the glassy carbon electrode to further enhance the ECL signal of luminol by promoting decomposition of H 2 O 2 into reactive oxygen species (ROSs). In addition to the horseradish peroxidase (HRP) catalysis effect on the luminol ECL signal, a triple amplified ConA sandwich ECL sensor with high sensitivity sensor was constructed. The linear range for ConA detection was from 0.5 pg/mL to 100 ng/mL with a detection limit of 0.3 pg/mL ( S / N = 3). The recovery test for ConA in human serum samples was performed with satisfactory results.
Graphical abstract
研究领域:
生物传感器
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分析化学
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电化学
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PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
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2021
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IF 4.598
Zhaoquan Zheng,Bingyu Wang,Jing Chen,Yan Wang,Ziyue Miao,Cenyao Shang,Qiang Zhang
Glycopolymer has been developed in bactericidal applications, but the traditional synthesis, such as click chemistry, needs poly-step reactions and complicated processes. Herein, incorporating the glyco-units into the polymers by quaternization has been exploited to give a facile strategy by Copper-mediated reversible deactivation radical polymerization (Cu(0)-RDRP) to prepare quaternized poly(ionic liquid)s (PILs) with pendant saccharide. The obtained PILs have the suitable property of killing typical Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus . Simultaneously, they perform the lower hemolytic rate of blood cells, higher selectivity index against bacteria, and higher affinity with Concanavalin A (ConA) than those quaternized by bromohydrin at the same quaternization degree. This result demonstrated that the introduced saccharide units, such as glucose and mannose, could maintain the electrostatic interaction to kill bacteria, perform lectin recognition behavior and interaction with ConA, and relatively harmlessness against red blood cells. Moreover, the sugar-containing PILs became insoluble and still antibacterial after anion exchange reaction. These new findings illuminated that such quaternized antibacterial agents with saccharide units that are easy-to-prepare, low cytotoxic could pave the way for further water disinfectant and pathogen control application.
研究领域:
生物材料学
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高分子化学
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微生物学
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2018
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IF 3.141
Rongting Hu,Guang Yang,Hong-ming Ding,Jinbiao Ma,Yu-qiang Ma,Jianhua Gan,Guosong Chen
We introduced a small molecular “inducing ligand” strategy to crystallize proteins via dual noncovalent interactions. Here we demonstrate that the variant protein-packing frameworks of concanavalin A (ConA) binding with ligands are controlled by different crystallization methods. Besides, the protein crystalline frameworks are also controlled by small-molecule inducing ligands RnM (n = 1–5), which varies in the number of ethylene oxide repeating units. To better understand the mechanism of different packing frameworks controlled by different inducing ligands, all-atom molecular dynamic (MD) simulations were performed. The MD simulation focused on the dynamic dimerization behavior of ConA-RnM system, which revealed clear relationships between the length of inducing ligands and ConA crystalline. In short, besides protein-packing framework control via different crystallization methods, inducing ligands RnM with suitable spacer lengths (n = 3, 4) also play an important role in desired and stable crystalline frameworks.
研究领域:
生物化学
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结构生物学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Xiaojian Li,Yaoguang Wang,Li Shi,Hongmin Ma,Yong Zhang,Bin Du,Dan Wu,Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
研究领域:
生物传感
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2026
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IF 5.7
Min Cai,Ruijuan Qi,Muqing Wang,Congyu Wu,Qian Chen,Yilin Guo,Yu Liu,Xin Huang,Yuan Gao,Yun Qi
DET decreases the transcription and translation of IL-2 in ConA-activated T cells. • DET inhibits ConA-activated NF-κB pathway, but fails to suppress MAPK signaling. • DET dampens ConA-induced nuclear translocation of NFAT. • DET directly suppresses calcineurin (CaN) activity not by reducing cytoplasmic Ca 2+ . • DET lowers serum IL-2 level of anti-CD3 antibody-treated mice. Deoxyelephantopin (DET) is a sesquiterpene lactone isolated from Elephantopus scaber , a traditional medicinal plant valued for its heat-clearing and detoxifying properties. As one of the major bioactive components, DET exhibits a variety of pharmacological activities, such as antitumor, antimicrobial, and anti-inflammatory effects. Nevertheless, its effect on T cell activation remains underexplored. Our study indicated that DET suppressed the proliferation and interleukin-2 (IL-2) production in T cells activated by various stimuli for T cell receptor (TCR)/CD3 signaling (i.e., concanavalin A (ConA), anti-CD3 antibody, and allogeneic major histocompatibility complex (MHC)). Mechanistically, DET lowered IL-2 mRNA level without affecting its stability in ConA-activated Jurkat T cells. Among three nuclear transcriptional pathways (mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB), nuclear factor of activated T cells (NFAT)) triggered by ConA, DET did not suppress MAPK signaling, but significantly inhibited NF-κB activation by hindering inhibitor of kappa-B alpha (IκBα) phosphorylation and degradation, thereby preventing p65 nuclear translocation. Concurrently, DET could dampen the activation of NFAT signaling through inhibiting the activity of calcineurin (CaN) to impede NFAT nuclear translocation independent of decreasing cytoplasmic Ca 2+ (Ca 2+ [c] ). Unlike classical CaN inhibitors (CNIs), DET inhibits CaN activity without involving immunophilins. In vivo , DET could also reduce serum IL-2 level in acute T-cell activation mouse model induced by intravenous injection of anti-CD3 antibody. In conclusion, our findings reveal DET's dual mechanisms (NF-κB and NFAT inhibition) responsible for repressing IL-2 expression in the activated T cells, which may provide a promising candidate for the development of a novel IL-2 suppressive agent. Download: Download high-res image (270KB) Download: Download full-size image
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PubMed
高影响因子
Botanical Studies
·
2016
·Vol. 57
·期 1
·1-11
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IF 1.159
Shi Qiang-Qiang,Dang Jun,Wen Huai-Xiu,Yuan Xiang,Tao Yan-Duo,Wang Qi-Lan
Background
Dracocephalum heterophyllum was a traditional Tibetan medicine possesses various pharmacological effects involved in anti-inflammatory, antibacterial activities. However, its anti-hepatitis, antioxidant activity and bioactive compounds have not been reported, the objective of this research work was to investigate the pharmacological activity and bioactive compounds of D. heterophyllum extracts. Results In the present study, the anti-hepatics and antioxidant activities of four D. heterophyllum extracts (i.e. petroleum ether extracts, ethyl acetate extracts, n -BuOH extracts, and water extracts) were conducted. The main chemical constituent of petroleum ether and ethyl acetate extracts were also isolated using chromatographic techniques and identified by NMR spectroscopic methods. The anti-hepatitis assay showed that the petroleum ether and ethyl acetate extracts of D. heterophyllum significantly prolonged the mean survival times and reduced the mortality of mouse hepatitis model induced by concanavalin A (ConA). The levels of alanine transaminase, aspartate transaminase in blood serum could be decreased obviously by ethyl acetate extracts compared with ConA group (P < 0.01). The histological analysis demonstrated that the ethyl acetate extracts could inhibit apoptosis and necrosis caused by ConA. In addition, the antioxidant activities of the four extracts of D. heterophyllum were measured by DPPH assay, ABTS assay, anti-lipidperoxidation assay, ferric reducing antioxidant power assay, ferrous metal ions chelating assay and determination of total phenolic contents. The results showed that the ethyl acetate extract had the highest antioxidant activities, followed by petroleum ether extract. Finally, nine mainly compounds were isolated from the Petroleum ether and ethyl acetate extracts, including four triterpenes: oleanolic acid ( 1 ), ursolic acid ( 2 ), pomolic acid ( 3 ), 2α- hydroxyl ursolic acid ( 4 ), three flavonoids: apigenin-7- O -rutinoside ( 5 ), luteolin ( 8 ), diosmetin ( 9 ) and two phenolic acids: rosmarinic acid ( 6 ), methyl rosmarinate ( 7 ). Conclusion The Ethyl acetate extract of D. heterophyllum had the highest anti-hepatitis and antioxidants activities, followed by petroleum ether extract. The bioactive substances may be triterpenes, flavonoids and phenolic acids, the ethyl acetate extracts of D. heterophyllum may be possible candidates in developing anti-hepatitis medicine.
研究领域:
药理学
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植物学
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药物化学
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PubMed
高影响因子
ACS Applied Nano Materials
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2020
·
IF 0
Jing Zheng,Jianping Wang,Dandan Song,Jingli Xu,Min Zhang
An ultrasensitive electrochemical sensor was developed for the quantitative determination of carcinoembryonic antigen (CEA), which is often utilized as a tumor marker and a prognostic indicator. In the paper, first, copper silicate integrated with nitrogen-doped magnetic carbon microtubes (NCMTs@Fe3O4@Cusilicate) was synthesized and employed to enrich concanavalin A (ConA), which can be used as an affinity probe to selectively capture CEA. Through chemical chelation between a copper ion and Con A, the combination of Cusilicate/ConA was greatly improved. Furthermore, gold nanoclusters (AuNCs) immobilized with aptamers were provided for signal amplification. Thus, in the presence of the target protein, CEA, in a sandwich manner, was formed, and AuNCs were gathered on the electrode through a magnetic field. Electrochemical oxidation of AuNCs to AuCl4– was performed, and immediately differential pulse voltammetry was performed, resulting in an electrochemical response because of the reduction of AuCl4–. On the basis of these merits, the constructed sensing system provided a ultrasensitive detection scheme for CEA with a linear range from 0.03 to 6.00 ng·mL–1 and a low detection limit of 5.38 pg·mL–1. In addition, the proposed strategy was employed to detect CEA in serum, which showed excellent correlations with standard immunoassay. The excellent performance indicated that the proposed sensor has significant potential for clinical diagnosis.
研究领域:
生物医学工程
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肿瘤标志物检测
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纳米技术
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DOI
危险品化学品经营许可证（带存储）