PROCESS BIOCHEMISTRY
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2017
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IF 2.497
Jinsha Huang,Wei Zhuang,Ce Wei,Liwen Mu,Jiahua Zhu,Yudan Zhu,Jinglan Wu,Yong Chen,Hanjie Ying
Orientation immobilization of enzymes has attracted intensive interest owing to the retainable specific activity and stability. Specially, glycoprotein immobilized onto Concanavalin A (Con A) modified carriers induces the orientation of the enzyme. However, the effects of the interface properties of carriers and enzymes are still not well understood yet. In this study, we synthesized the activated porous poly (styrene- divinylbenzene) resin carriers with 30 nm pore sizes and 72 m 2 g −1 specific surface areas and decorated with Con A. The resultant loading capacity of NP 1 on Con A modified carriers was as high as 4.02 mg g −1 wet support as a result of strong affinity between the enzyme and Con A decorated on carriers. It was found that the acid resistance, thermal stability, reusability and degradation efficiency of the immobilized enzyme on Con A modified porous carriers were significantly improved. The reduction of K m from 18.40 ± 0.55 mg mL −1 to 17.19 ± 0.51 mg mL −1 illustrated the improved substrate affinity of HA-GA-ConA-NP 1 . Moreover, Con A-affinity NP 1 exhibited the best operational stability that only 7% of its initial activity was lost even after 9 batches repeated reaction. This work demonstrates that surface property manipulation of porous carriers and its derivatives has great potential in efficient biocatalytic systems.
研究领域:
生物化学
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酶工程
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
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2024
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IF 27.4
Yinli Jin,Shuhua Liu,Xueyang Wang,Chenyuan Wang,Qiongfang Ruan,Wei Li
Chronic wounds impose significant physical and mental burdens on patients. Nano-based formulations offer a promising strategy for chronic wound healing due to their non-invasive nature and enhanced biofilms penetration, but they often lack targeting capability or fail to achieve long-term and synergistic effects. In this work, a multifunctional microneedle (MN) patch loaded with engineered nitric oxide (NO)-releasing nanocarriers are presented that encapsulate an antibacterial agent and are immobilized with Concanavalin A (Con A) and NO molecules for targeted and synergistic treatment of chronic wounds. With the assistance of MNs, the nanoparticles (NPs) can directly cross bacterial biofilms and be efficiently delivered to wound tissues, where they target harmful bacteria through the specific recognition between Con A and polysaccharides on bacterial surfaces, followed by the release of the encapsulated antimicrobial agent, thereby achieving effective antibacterial effect. Moreover, the NPs generate NO in a sustained manner as they dissociate in the wound tissue, which exerts potent anti-inflammatory action and benefits tissue regeneration, further promoting chronic wounds closure. Consequently, this work provides a novel MN patch loaded with engineered NPs designed for accelerating chronic wound healing through targeted and synergistic therapy.
研究领域:
生物医学工程
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药剂学
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伤口护理
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DOI
PubMed
高影响因子
RSC Advances
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2015
·Vol. 5
·期 73
·59603-59608
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IF 3.84
Shuguang Yan,Yurong Tang,Mengling Yu
Herein we developed a highly sensitive methodology for heparin detection based on a resonance Rayleigh scattering (RRS) spectral assay. Heparin and concanavalin A (conA) densely stacked together and formed nanoparticles or a well-defined brick-and-mortar nanostructure. A strong electrostatic attraction, hydrogen bond and the solid–liquid interface induced RRS enhancement. The result of absorption spectra and TEM testified that the introduction of heparin indeed changed the structure of conA and formed brick-and-mortar nanostructures. The optimum reaction conditions of the method and the reasons for RRS enhancement are also discussed. Combined with an RRS spectral assay, an effective biosensor has been developed for heparin. The bioassay allows sensitive detection of heparin with a detection limit of 2.48 ng mL−1 (3σ), a linear range from 8.28 ng mL−1 to 2.5 μg mL−1. The method was successfully applied to the determination of heparin in heparin sodium injection samples.
研究领域:
分析化学
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2015
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IF 5.636
Liping Dong,Shun Feng,Shanshan Li,Peipei Song,Jide Wang
In this work, a soft and nondestructive approach was developed to prepare concanavalin A-chelating magnetic nanoparticles (Con A-MNPs) for selective enrichment of glycoproteins. Ethylenediamine tetraacetic acid-modified-MNPs (EDTA-MNPs) were prepared by a one-pot chemical coprecipitation method first, and then, Cu(II) cations were used as bridge groups to immobilize Con A on EDTA-MNPs. The as-prepared absorbents with a mean diameter of 15 nm showed a strong magnetic response to an externally applied magnetic field. The results of thermogravimetric analysis showed the content of immobilized Con A was up to 28 wt %. For glycoprotein ovalbumin, the maximum capacity and equilibrium constant were 72.41 mg/g and 0.6035 L/mg, respectively. The as-prepared nanocomposites exhibited a remarkable selectivity for glycoproteins and can enrich glycoproteins specifically from a mixture of glycoprotein and nonglycoprotein even at a molar ratio of 1:600. It was also successfully applied for the enrichment of glycoproteins from real egg white samples. We expect that our finding will serve as a helpful template for others to design new adsorbents for enriching glycoproteins.
研究领域:
生物化学
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纳米技术
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蛋白质组学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2011
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IF 2.502
Ruixue Yin,Zi Tong,Dongzhi Yang,Jun Nie
Glucose and pH dual-responsive microhydrogels based on concanavalin A (Con A) were prepared and used for insulin delivery. The combination of the specific saccharide-binding affinity of Con A and the cationic groups of N-(2-(dimethylamino) ethyl)-methacrylamide (DMAEMA) led to dual-responsive systems. SEM, fluorescence microscopy and particle size analysis showed that the obtained microhydrogels had a dense surface morphology and an average size of 38 μm. The in vitro insulin release study revealed that the microhydrogels could quickly respond to the changes of glucose concentrations in the medium and small change in pH value of the environment. The kinetics of insulin release was analyzed by using empirical equation and the apparent diffusion coefficient was calculated according to a solution of Fick's second law. The released insulin was proved to remain active. The result suggested that this microhydrogel might find potential applications for self-regulated insulin delivery, actuators and separation systems with sensitivity to glucose.
研究领域:
生物材料学
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药物递送系统
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Ya Li,Yao Lu,Yang Chen,Pei Zhang,Nengqin Jia
A fullerene-promoted organic-inorganic hybrid electrochemiluminescence (ECL) biosensor was proposed for sensitive detecting concanavalin A (ConA). Here, platinum-modified inorganic TiO 2 (Pt-TiO 2 ) promotes charge transfer to enhance ECL intensity of organic luminescent probe—ammonolysis product of PTCDA (PTC). Fullerene interacts with K 2 S 2 O 8 co-reagent to produce more SO 4 •− free radicals , and its large specific-surface-area and electrophilic nature are beneficial to loading more luminescent PTC. The glucose oxidase (GOx) is chosen for specific recognition of ConA through a biospecific interaction. The constructed ECL biosensor shows a wide detection range of 1 × 10 −4 –1 × 10 3 ng mL −1 with a low detection limit (LOD) of 1.8 × 10 −5 ng mL −1 , as well as high stability, reproducibility, and detection capability of actual serum. This work provides a promising organic-inorganic hybrid biosensor platform for early biotherapy and clinical diagnosis.
研究领域:
生物传感
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分析化学
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医学诊断
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2024
·
IF 4.1
Xiaojuan You,Yumei Chen,Jingming Zhou,Hongliang Liu,Yankai Liu,Yanhua Qi,Chao Liang,Peiyang Ding,Xifang Zhu,Aiping Wang,Gaiping Zhang
Concanavalin A (ConA), as a carrier for immobilizing antibodies, was used for the first time in electrochemical immunosensor. In this work, a label-free electrochemical immunosensor based on Ag nanoparticles-reduced graphene oxide (AgNPs-rGO) nanocomposite and ConA was successfully prepared to detect arsanilic acid (ASA). AgNPs-rGO nanocomposite displayed superior conductivity and redox capability. Specially, ConA showed excellent binding ability with monoclonal antibody of ASA through lectin-sugar interactions. Under the optimized conditions, the proposed electrochemical immunosensor showed a linear dependence over ASA concentrations ranging from 0.025 ng/mL to 25 ng/mL with a low detection limit of 0.011 ng/mL. Furthermore, the immunosensor was used to monitor ASA in chicken and egg with good precision and accuracy. In conclusion, the simple and low-cost immunosensor was desirably applied as a useful candidate for detection of ASA in animal derived food.
研究领域:
分析化学
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纳米技术
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食品科学
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
Results in Chemistry
·
2025
·
IF 4.2
Yaan Zhang,Tingyu Zhou,Zhuxin She,Wei Feng,AnJie Ju,Yi Li,Yuanyuan Liu
Multivalent lectin-glycan interactions play a significant role in biology. Developing novel methods to investigate glycosyl-receptor interactions quantitatively and elucidate binding modes is crucial for advancing biomedical detection techniques and innovative therapeutic approaches. Herein, a mannobiose-chalcone disulfide ligand ( 2S-DiMan ) was designed and synthesized. Through click chemistry and surface ligand exchange, 2S-DiMan-functionalized gold nanoprobes ( 2S-DiMan-AuNPs ) were prepared and characterized. Density functional theory (DFT) calculations and molecular docking revealed that the oxygen atoms of the mannobiose moiety in 2S-DiMan have the highest negative charge, enabling specific recognition of the carbohydrate recognition domain (CRD) of concanavalin A (Con A) by effectively occupying its sugar-binding site and forming hydrogen bonds. Thus, the 2S-DiMan ligand exhibits strong binding affinity for Con A. The effect of fluorescence quenching on the recognition of Con A was further investigated. Obvious quenching was observed upon 2S-DiMan-AuNPs addition. The probes showed a dissociation constant ( K d ) of 0.76 μM for Con A (affinity enhancement factor β = 315-fold vs. monovalent mannose) and achieved a limit of detection as low as 5.02 nM. This work provides a novel structure and method for analysing lectins with sugar-binding sites, investigating protein-glycan multivalent binding affinity, and assaying disease-associated glycans, with promising potential for medical research and biotechnological applications.
研究领域:
糖生物学
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纳米生物技术
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分子识别
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生物分析化学
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结构生物学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Yilin Li,Shupei Zhang,Hong Dai,Zhensheng Hong,Yanyu Lin
This work designed a delicate signal-on photoelectrochemical sensing methodology for concanavalin A monitoring by coupling graphene-supported quasi-octahedral TiO 2 mesocrystal with an efficient enzyme-free labeling strategy. Herein, graphene with prominent conductivity could accelerate photo-generated electrons transfer, thus enhancing the photocurrent response. Currently, labeling of various enzymes was the mainly strategy for signal amplification, unfavorably, this method usually suffered inferior stability. Hence, the exploration and development of NiCo 2 O 4 labeling was proposed here, which could effectively accept the photo-induced electrons from conduction band of TiO 2 mesocrystal, leading to the decrease of the photocurrent intensity. Accordingly, a signal-on non-enzymatic photoelectrochemical sensor was constructed based on immobilized concanavalin A competing with isolated concanavalin A of various concentrations for reaction with the NiCo 2 O 4 labeled glucose conjugates. Under the optimized conditions, the photocurrent increased with the increasing concanavalin A concentration in a dynamic linear range from 0.05 ng/mL to 0.5 μg/mL, thereby proposing a general format for practical use in quantitative determination of concanavalin A. Promisingly, the successful consideration of graphene sensitized TiO 2 mesocrystal further broadened the application of excellent photoactive materials for photoelectrochemical sensing platform.
研究领域:
分析化学
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材料科学
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生物化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
·
IF 5.401
Xionghui Ma,Jianping Li,Yunbin Liu,Yali Yuan,Guobao Xu
Herein, a concanavalin A (ConA) electrochemical sensor was constructed via a sandwich mode. Firstly chitosan functionalized graphene oxide (CS/GO) composite was introduced on the of glassy carbon electrode (GCE) as the substrate. D–mannose (D-man) was immobilized on the surface of CS/GO via Schiff base reaction. Due to the specific affinity between mannose and ConA, the prepared D–man/CS/GO sensing interface could specifically capture ConA which could further react with mannose residues of HRP and form a sandwich configuration. Based on the catalytic effect of horseradish peroxidase (HRP) to H 2 O 2 , a ConA electrochemical sensor with enzyme catalytic amplification was constructed by taking hydroquinone as electrical mediator. The morphology of CS/GO was investigated by transmission electron microscope and Fourier transform infrared spectroscopy. Electrochemical techniques including electrochemical impedance spectroscopy and differential pulse voltammogram (DPV) were used for reveal the characteristics of the sensing platform. Benefit from the high surface area of GO and enzyme catalytic amplification of HRP, the peak current from DPV is linearly dependent on the ConA concentration ranging from 5 × 10 −9 to 5 × 10 −7 mol/L with a detection limit of 1.24 × 10 −9 mol/L. The results demonstrated that the proposed sensor might exploit a new direction for determination of ConA.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenze Lu,Gongyan Wang,Xiaoxiao Zhang,Zhihui Fang,Xinyuan Wang,Fan Tang,Kang Ning,Min Xu,Jing Wang,Han Jiang,Ran Tao,Pei Xu
Intake of Concanavalin A (Con A), a hazardous lectin protein commonly discovered in legume vegetables, could cause various systemic symptoms including rash, nausea, vomiting, diarrhea, etc. Herein, we present a novel label-free and cost-effective electrochemical Con A aptasensor. [Fe(CN) 6 ] 3−/4- ions in testing buffer not only generate redox peaks during electrochemical scan, but also serve as electron acceptor/donor to enhance electron transfer. Anti-Con A aptamer is immobilized on surface of screen printed electrode to capture Con A molecules and form aptamer-protein complex, which hinders electron transfer near electrode surface and causes a decrease in peak current value. The constructed aptasensor displays a low detection limit of 17.88 ng/mL (around 171.92 pM), as well as good specificity against other legume lectins. The practicality of electrochemical sensors was evaluated using real-world samples, demonstrating their ability to rapid detect Con A in crude protein extracts from jack bean seeds. This work provides novel tools for rapid evaluation of Con A content in massive samples, which is required in food safety inspection and safety breeding of legume vegetables.
研究领域:
生物传感技术
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食品安全
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Sijie Yuan,Ying Tu,Ru Yu,Fei Nie
Introducing novel catalysts is essential for developing chemiluminescence (CL) systems that exhibit sustained and robust emission. Traditional Luminol-H2O2 systems typically feature flash-type CL emission. In this study, we discovered that the porous material HKUST-1 can induce a long-lasting and intense CL emission when combined with Luminol-H2O2. This long-term emission signal can be directly detected by the smartphone. By changing the calcination temperature, a series of microporous and hierarchically porous HKUST-1 materials were prepared as catalysts to adjust the kinetic characteristics of the CL signal of Luminol-H2O2 system from flash-type to glow-type. A systematic investigation into the influence of the central metal and ligand, aperture, and particle size of HKUST-1 on the CL kinetic properties revealed that the pore structure has the most pronounced impact on the dynamics of the Luminol-H2O2 CL reaction. Capitalizing on the intense emission of the HKUST-1-catalyzed Luminol-H2O2 system, we established a CL sandwich immunoassay strategy for concanavalin A (ConA), demonstrating good linearity and low detection limit. This research presents a significant endeavor in modulating the dynamics of CL signal emissions.
研究领域:
分析化学
·
生物传感
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）