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Coprinus comatus
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY · 2023 · IF 2.9

Optimization of liquid fermentation conditions for Coprinus comatus to enhance antioxidant activity

Dongyu Gu,Shanshan Tang,Chang Liu,Dajun He,Jing Tian,Yi Yang

Coprinus comatus is an edible and medicinal fungus. In this study, the antioxidant activity of the fermentation product of C. comatus was investigated through optimization of fermentation process. The results indicated that the fermentation product of C. comatus had obvious scavenging ability for 2,2’-Azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) free radical. The EC50 of the n-butanol extract from the fermentation product on ABTS·+ was 0.65 ± 0.02 mg/mL. On this basis, the liquid fermentation conditions of C. comatus were optimized through single factor and response surface optimization experiments according to the scavenging ability of ABTS·+ to improve the antioxidant capacity of the fermentation product. The results showed that when the 14% of C. comatus was fermented in a culture medium with a C/N ratio of 48:1 for 6 days, the ABTS·+ scavenging ability was the strongest, and the EC50 of n-butanol extract was 0.57 ± 0.01 mg/mL, which was 12.31% higher than the initial activity. This study laid the foundation for the development of C. comatus.

CAS / MDL: 28752-68-3
研究领域: 微生物学 · 食品科学 · 生物技术
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY · 2024 · IF 2

Immobilization of Coprinus comatus with magnetic alginate hydrogel microsphere for improving the antioxidant activity of fermentation products

Shanshan Tang,Jia Zhao,Chang Liu,Dezhi Huang,Jing Tian,Yi Yang

Coprinus comatus is an edible mushroom and its fermented product possesses antioxidant activity. In this study, to further enhance the antioxidant activity and improve the reusability of the strain, calcium alginate hydrogel was used as the carrier for embedding and immobilizing Coprinus comatus. The effects of CaCl2 concentration, sodium alginate concentration, microsphere diameter, and the amount of magnetic particle on the antioxidant activity of fermented products were investigated. The results showed that the magnetic immobilized microsphere prepared by 2.50% CaCl2, 2.00% sodium alginate and 0.50% Fe3O4 had the best fermentation antioxidant activity (EC50 was 0.43 ± 0.01 mg/mL) when the diameter was 5 mm, which increased by 24.56% compared to the initial activity. Besides, the microsphere showed strong reusability, the antioxidant activity was still better than the free strain after being used five times. This study not only enhanced the antioxidant activity of Coprinus comatus fermented product through immobilization, but also provided an effective method for microbial fermentation.

CAS / MDL: 28752-68-3
研究领域: 微生物学 · 食品科学
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2022 · IF 6.056

Evaluation of physicochemical properties, equivalent umami concentration and antioxidant activity of Coprinus comatus prepared by different drying methods

Shiya Ren,Enping Zheng,Tingxue Zhao,Shunqiang Hu,Hailong Yang

Coprinus comatus contains a variety of bioactive and umami compounds and is regarded as “the rising star of fungi”. To maximally maintain its quality, the physicochemical and antioxidant properties of C. comatus were investigated and compared, after undergoing hot air drying (HAD), infrared drying (IRD), microwave drying (MD) and freeze drying (FD). Results showed that different drying methods have their respective advantages. FD sample showed the lowest color difference and highest rehydration ratio, as well as provided the highest retention of gallic acid (223.76 μg/g) and protocatechuic acid (336.67 μg/g), thereby demonstrating highest free radicals (DPPH· and ABTS·) scavenging activity. MD significantly retained polysaccharide (113.36 mg/g), coumaric acid (13.36 μg/g) and p-hydroxybenzoic acid (35.09 μg/g), and exhibited higher ferric reducing antioxidant power. IRD and HAD led to higher contents of monosodium glutamate-like free amino acids and flavor 5′-nucleotide. In addition, IRD sample had the highest equivalent umami concentration value (1145.73 g MSG/100 g). Comprehensively, FD was better to preserve the antioxidants, while IRD would be a potential method to produce dried C. comatus with high umami concentration.

研究领域: 食品科学 · 营养学 · 生物化学 · 农业工程
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NANO · 2017 · IF 1.025

Coprinus Comatus-Based Nitrogen-Doped Active Carbon for High Performance Supercapacitor

Guofu Ma,Wei Tang,Kanjun Sun,Zhiguo Zhang,Enke Feng,Ziqiang Lei

Coprinus comatus-based nitrogen-doped activated carbon (N-ACC) is prepared by chemical activation and nitrogen-doped methods. The N-ACC possesses large specific surface areas (976.96 m 2 g − 1 ), high nitrogen contents (11.53 wt.%), and super hydrophilicity. As electrode material for supercapacitors, the N-ACC shows remarkable electrochemical performance, such as 346 F g − 1 maximum specific capacitance at a current density of 1 A g − 1 , which retains 260 F g − 1 even at a high current density of 10 A g − 1 (about 75% capacitance retention) in 2 M KOH aqueous electrolyte. The assembled N-ACC//N-ACC symmetric supercapacitor exhibits energy density of 14.63 Wh kg − 1 at power density of 810 W kg − 1 , and excellent cycling stability with 92% specific capacitance retention after 10000 cycles in the voltage range 0–1.8 V in 0.5 M Na 2 SO 4 aqueous solution. These results indicate that the N-ACC as electrode materials can be used for high performance supercapacitors.

CAS / MDL: 57-13-6
研究领域: 材料科学 · 能源技术
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