JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2019
·Vol. 56
·期 2
·1027-1036
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IF 1.85
Yang Shuang,Liu Jingsheng,Zheng Mingzhu,Zhao Chengbin,Cao Yong,Dong Yanjiao,Yaqoob Sanabil,Xiao Yu,Xu Xiuying
Cordyceps militaris ( C. militaris ) was utilized to ferment cornmeal by solid state fermentation. The main objective of this study was to investigate effect of fermentation on the dynamic state of water and microstructure distribution of water within cornmeal with Low-field nuclear magnetic resonance, as well as the effect on composition and microstructure properties. The spin–spin relaxation time (T 2 ) showed significant changes in solid-state fermented cornmeal. Principal component analysis further revealed that the variations within different fermentation stage could be discriminated by the T 2 parameters. Bulk water (T 22 ) was the main form of water present and lost in substrates. The weights of different indicators, as assessed by multiple regression analysis, demonstrated that there was a strong correlation between starch and T 2 relaxation. Scanning electron microscopy demonstrated that fermentation can cause the appearance of micropores. The longer relaxation time of T 22 during logarithmic period can be interpreted as a loosening of the structure at the starch hydrolysis, introducing more water into the structure. Thus, the differences in composition and structure of the substrate at different fermentation time produce different T 2 values.
研究领域:
食品科学
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微生物学
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PubMed
高影响因子
Foods
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2025
·Vol. 14
·期 15
·2711
·
IF 5.1
Wanying Song,Xinyue Zhang,Huiyi Yang,Hanyu Liu,Baodong Wei
This study presents the development and optimization of a functional soy sauce fermented withCordyceps militaris(C. militaris), a medicinal fungus known for its high cordycepin and polysaccharide content. UsingC. militarisas the sole starter culture, the process aimed to improve both nutritional and functional properties. Response surface methodology was employed to optimize the entire fermentation process. During the koji stage, temperature, aeration, and inoculum concentration were adjusted to maximize protease activity and cordycepin production. In the fermentation stage, temperature, brine concentration, and water-to-material ratio were optimized to increase amino acid nitrogen and bioactive compound levels. Under optimal conditions (24 °C, 679.60 LPM aeration, 9.6% inoculum for koji; 32 °C, 12% brine, 1.53:1 water-to-material ratio for fermentation), the resulting soy sauce contained 1.14 ± 0.05 g/100 mL amino acid nitrogen and 16.88 ± 0.47 mg/100 mL cordycepin. Compared with traditionally fermented soy sauce, theC. militarisproduct exhibited a darker color, enhanced umami taste, and a distinct volatile profile featuring linoleic acid, methyl palmitate, and niacinamide. These results demonstrate the feasibility of usingC. militarisin soy sauce fermentation and its potential as a novel functional condiment with improved bioactivity and sensory quality.
研究领域:
食品科学
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微生物学
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生物技术
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营养学
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发酵工程
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JOURNAL OF THE TEXTILE INSTITUTE
·
2023
·
IF 1.7
Jie Chen,Qing Suo,Yu Ni,Jiajing Yan,Yunli Wang,Huiyu Jiang,Huan Qi
As a sustainable and ecological dye resource, the biomass materials are receiving more and more attention. In this work, the pigments in Cordyceps militaris were extracted and applied for dyeing wool fabrics, and the various properties were investigated. The extracted pigments and dyed wool fibers were analyzed and characterized by ultraviolet–visible (UV-Vis) spectroscopy, Liquid Chromatography-Mass Spectrometry (LC-MS) and Fourier Transform Infrared (FTIR) spectroscopy. The single-factor experiments were used to screen and optimize the dyeing process. Six metal mordants with different use order were compared with each other, and their possible mordanting mechanisms were proposed. The color fastnesses, UV resistance and antioxidant properties were evaluated after dyeing. The results showed that UV-Vis, LC-MS and FTIR analysis confirmed that the main components of C. militaris extract was lutein. The optimal dyeing process for the extract was as follows: dyestuff mass 200% (o.m.f.), dyeing temperature 90 °C, dyeing time 45 min, bath pH 5.0, Na2SO4 mass 0 g/L. Metal ions are added to form chelate bonds between the dyes and the fibers, further improving the color fastness. The dyed fabric exhibits excellent UV resistance and its UPF (Ultraviolet Protection Factor) value increases with the color yield. Meanwhile, the dyed wool fabric could exhibit up to 94.77% antioxidant activity. These results provide new insight for the application of C. militaris pigments on wool fabric.
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Food Science and Technology
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2020
·
IF 1.443
LI Li,ZUO Jin-Hui,YI Fan,YANG Yun-Li,DONG Yin-Mao,LI Qin-Yu,LI Min-Hui
Cordyceps militaris is a commonly used edible and medicinal fungus in East Asia. Preparation of the mycelium and fermentation liquid by submerged fermentation using additives such as Panax ginseng for skin whitening applications has attracted wide attention. In the present study, we screened traditional Chinese medicines as additives to the liquid fermentation medium for C. militaris and performed systematical research on the changes of bioactivity and ginsenoside levels during the fermentation process. Total polysaccharide contents, cordycepin content, and polysaccharide molecular weight in the fermentation products were measured by UV spectrophotometry, high-performance liquid chromatography-diode array detection (HPLC/DAD), and gel permeation chromatography-multi angle laser light scattering (GPC-MALLS), respectively. In addition, the ginsenosides Rb1, Re, Rd, Rg1, and pseudoginsenoside F11 in the fermentation products were quantitatively analyzed by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). Panax ginseng significantly increased the cordycepin and total sugar contents, and the average molecular weight of polysaccharides in the fermentation liquid and the mycelium of C. militaris. At the same time, C. militaris enhanced the production of ginsenoside pseudoginsenoside F11, which is a unique component of Panax quinquefolium. The research provides scientific evidence for the application of C. militaris and P. ginseng as raw materials for food and medicines.
研究领域:
药用真菌学
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生物化学
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中药学
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高影响因子
Processes
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2024
·Vol. 12
·期 4
·687
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IF 2.8
Yannan Xiang,Siyi Tian,Xinyu Luo,Chenggang Cai,Yaowen Du,Hailong Yang,Haiyan Gao
The content of differentially abundant metabolites in the fermentation broth of grapefruit peels fermented byCordyceps militarisat different fermentation times was analyzed via LC–MS/MS. Small molecule metabolites and differential metabolic pathways were analyzed via multivariate analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. A total of 423 metabolites were identified at 0, 2, 6, and 10 days after fermentation. Among them, 169 metabolites showed differential abundance, with significant differences observed between the fermentation liquids of every two experimental groups, and the metabolite composition in the fermentation liquid changed over the fermentation time. In summary, the upregulation and downregulation of metabolites in cancer metabolic pathways collectively promote the remodeling of cancer cell metabolism, facilitating increased glycolysis, alterations in TCA cycle flux, and enhanced biosynthesis of the macromolecules required for rapid proliferation and survival. This study provides new perspectives on the development of high-value-added agricultural and forestry byproducts and the development and research of functional foods.
研究领域:
食品科学
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微生物学
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代谢组学
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药理学
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高影响因子
Polymers
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2022
·Vol. 14
·期 21
·4636
·
IF 4.967
Zhengyu Hu,Jiaming Wang,Long Jin,Tieqiang Zong,Yuanqi Duan,Jinfeng Sun,Wei Zhou,Gao Li
This investigation focuses on the three novel polysaccharides fromCordyceps militarisand then discusses their characterization and anti-complementary activity. The three polysaccharides fromC. militaris(CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.Keywords:Cordyceps militaris;anti-complementary activity;polysaccharides;structure-activity relationship
研究领域:
免疫学
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生物化学
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PubMed
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Frontiers in Microbiology
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2018
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IF 4.019
Yong Tianqiao,Chen Shaodan,Xie Yizhen,Chen Diling,Su Jiyan,Shuai Ou,Jiao Chunwei,Zuo Dan
Recently, we've reported the anti-hyperuricemic effects of Cordyceps militaris. As a characteristic compound of C. militaris, we hypothesized that cordycepin may play a role in preventing hyperurecimia. Remarkably, cordycepin produced important anti-hyperuricemic actions, decreasing SUA (serum uric acid) to 216, 210, and 203 μmol/L (P < 0.01) at 15, 30, and 60 mg/kg in comparison of hyperuricemic control (337 μmol/L), closing to normal control (202 μmol/L). Elisa, RT-PCR and western blot analysis demonstrated that the actions may be attributed to its downregulation of uric acid transporter 1 (URAT1) in kidney. Serum creatinine levels and blood urine nitrogen and liver, kidney, and spleen coefficients demonstrated that cordycepin may not impact liver, renal, and spleen functions. In addition, we used computational molecular simulation to investigate the binding mechanism of cordycepin. Of which, van der Waals interaction dominated the binding. Residues TRP290, ARG17, ALA408, GLY411, and MET147 contributed mainly on nonpolar energy. This provided the theoretical guidance to rationally design and synthesis novel URAT1 inhibitors.
研究领域:
药理学
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生物化学
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分子生物学
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PubMed
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Nanoscience and Nanotechnology Letters
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2017
·Vol. 9
·期 10
·1453-1462
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IF 1.889
Wu Yulong,Zhou Feng,Jiang Haitao,Wang Zhengjiong,Hua Chun,Wang Renlei
Two novel polysaccharides fractions were isolated from Cordyceps militaris substrate polysaccharides (CMSP) by DEAE-52 cellulose and Sephadex G-100 column chromatography, and named as CMSP-1 and CMSP-2. The CMSP-1 was a heteropolysaccharide mainly composed of sorbin, D-mannose,
D-glucose, galactose, arabinose, with the molar ratios of 1.00:6.32:10.23:2.86:1.02, and the molecular weight of CMSP-1 was 10715.2 Da. The CMSP-1 was used to investigate the protective effect on acute ethanolic hepatotoxicity (AAH) in male mice. The results indicated that oral administration
of CMSP-1 could significantly decrease the liver index, and increase body weight in mice with AAH. CMSP-1 could also significantly increase the levels of SOD, GSH and GSH-PX, and decrease the levels of ALT, AST, TG, VLDL and MDA in AAH model mice. Moreover, Histopathological examination of
liver showed that CMSP-1 could significantly alleviate the symptoms of liver injury caused by ethanol, such as swelling of hepatocytes, loose cytoplasm and lipid changes. Therefore, CMSP-1 has obvious protective effect on AAH model mice, and has a good prospect in functional foods or natural
medicine.
研究领域:
药理学
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肝病学
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营养保健品
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Houle Xiang,Yuqing Zhang,Shuo Zhang,Jingwei Kou,Hui Wang,Fengxia Zou,Wei Zhuang,Pengpeng Yang,Jinglan Wu,Hanjie Ying
Cell disruption is essential for extracting intracellular proteins from fungal biomass. However, its effects on the structural and functional properties of proteins remain insufficiently understood. This study used Cordyceps militaris as a model to evaluate three cell disruption techniques—high-pressure homogenization (HPH), ultrasonication (US), and cellulase hydrolysis—for extracting C. militaris protein isolates (CMPI). All methods significantly improved protein extraction compared to alkaline extraction alone, with HPH yielding the highest rate (70.75 %, p < 0.05). Structural analysis revealed a decrease in α-helix and β-sheet content, along with increased β-turns, random coils, surface hydrophobicity, and modifications in disulfide–sulfhydryl balance. CMPI-HPH and CMPI-US showed superior water-holding capacities (6.23 and 5.95 g/g) and oil-holding capacities (9.97 and 7.70 g/g), as well as enhanced foaming (69.57 % and 65.22 %) and emulsifying activities (32.07 and 31.13 m 2 /g). CMPI-HPH also exhibited the highest foaming stability (87.50 %). In vitro digestibility was significantly greater in CMPI-US (80.05 %) and CMPI-HPH (71.71 %) than in the control ( p < 0.05). These results demonstrate that cell disruption, especially HPH, effectively improves protein extraction efficiency, structural flexibility, and techno-functional properties, offering new potential for the sustainable application of fungal proteins in food systems.
研究领域:
食品科学
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生物技术
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真菌学
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蛋白质工程
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结构生物学
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PubMed
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Redox Biology
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2024
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IF 10.7
Mengfei Chen,Zhenjun Zhu,Shujian Wu,Aohuan Huang,Zhiqing Xie,Jie Cai,Rong Huang,Shubo Yu,Ming Liu,Jumei Zhang,Yuchung Tse,Qingping Wu,Juan Wang,Yu Ding
Oxidative stress (OS) and disruption of proteostasis caused by aggregated proteins are the primary causes of cell death in various diseases. Selenopeptides have shown the potential to control OS and alleviate inflammatory damage, suggesting promising therapeutic applications. However, their potential function in inhibiting proteotoxicity is not yet fully understood. To address this gap in knowledge, this study aimed to investigate the effects and underlying mechanisms of the selenopeptide VPRKL(Se)M on amyloid β protein (Aβ) toxicity in transgenic Caenorhabditis elegans . The results revealed that supplementation with VPRKL(Se)M can alleviate Aβ-induced toxic effects in the transgenic C. elegans model. Moreover, the addition of VPRKL(Se)M inhibited the Aβ aggregates formation, reduced the reactive oxygen species (ROS) levels, and ameliorated the overall proteostasis. Importantly, we found that the inhibitory effects of VPRKL(Se)M on Aβ toxicity and activation of the unfolded protein are dependent on skinhead-1 (SKN-1). These findings suggested that VPRKL(Se)M is a potential bioactive agent for modulating SKN-1, which subsequently improves proteostasis and reduces OS. Collectively, the findings from the current study suggests VPRKL(Se)M may play a critical role in preventing protein disorder and related diseases.
研究领域:
神经科学
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生物化学
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药理学
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Zhongxu Sun,Zeyang Li,Qingping Wu,Huiying Huang,Yuanting Tang,Zimeng Wang,Qiao Ding,Hua Wei,Yu Ding,Zhenjun Zhu
Selenium (Se) enrichment changes the nutritional composition and secondary metabolites of Cordyceps militaris (CM), but its effect on the fermentation process of CM is still unclear. This research revealed the effects of Se enrichment on the nutritional and metabolic characteristics of Cordyceps militaris juice (CMJ) fermented by Lactobacillus DZ001 (API). The results indicated that Se enrichment significantly enhanced the growth and metabolic activity of API. Furthermore, Se enrichment showed a higher initial carbohydrate content and significantly increased the total peptide content in CMJ, while it reduced the degradation of polyphenols and ascorbic acid, indicating that Se mediated the antioxidant protection. In addition, the amino acid and fatty acid contents increased significantly, but the compositional diversity did not change. Untargeted metabolomics further revealed Se-induced upregulation of differential metabolites, which were related to pathways such as ascorbic acid and linoleic acid metabolism. Volatile metabolomics showed that Se enrichment considerably increased the content of terpenes, alcohols and esters in flavor metabolites, and significantly improved flavor diversity. Sensory evaluation confirmed that Se enrichment can improve the sensory properties of fermented CMJ. These results highlight the role of Se in improving nutritional components and enhancing flavor, making Se-CMJ a promising nutritional flavored beverage.
研究领域:
食品科学
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微生物学
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营养学
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生物化学
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生物技术
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DOI
高影响因子
CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Tao Xuan Vu,Minh-Trung Nguyen,Anh Ngoc Dao,Tram Bao Tran,Yen Thi Hoang Le,Van-Tuan Tran,Thu-Huong Nguyen,Quang-Vinh Nguyen
Cordyceps militaris is a medicinal fungus known for its immunomodulatory effects. This study compared the antioxidant and anti-inflammatory activities of an albino strain ( C. militaris SHBTQ) and its wild-type counterpart (SHQ) using ethanol extracts from their fruiting bodies. The albino strain exhibited significantly higher bioactive compound levels, with cordycepin and pentostatin concentrations 2.37- and 1.74-fold higher than in the wild type. Enhanced polyphenol and flavonoid content correlated with stronger 1,1-diphenyl-2-picrylhydrazyl and azino-bis-(3-ethylbenzothiazoline sulfonate) radical scavenging (2.67- and 1.91-fold higher) and superior copper-chelating ability (97.52% vs. 51.83% at 5 mg/L). It also demonstrated greater anti-inflammatory effects, with a lower 50% inhibitory concentration in the bovine serum albumin denaturation assay (7.42 vs. 14.49 mg/mL) and reduced oxidative stress in zebrafish models. The superior bioactivity of SHBTQ, likely due to its high bioactive compound content, underscores its therapeutic potential against oxidative stress-related diseases. Graphical
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危险品化学品经营许可证（带存储）