FOOD CHEMISTRY
·
2018
·
IF 4.946
Tong Bu,Qiong Huang,Lingzhi Yan,Wentao Zhang,Leina Dou,Lunjie Huang,Qingfeng Yang,Bingxin Zhao,Baowei Yang,Tao Li,Jianlong Wang,Daohong Zhang
Here, a facile, label-free and sensitive lateral flow strip (LFS) biosensor for foodborne pathogens was established relying on the innovative introduction of Gram staining and the direct immunoreaction. Target bacteria can be directly marked with crystal violet (CV) by one-step staining which is superior to traditional signal marking techniques in LFS assay, and the method’s selectivity can be guaranteed by high-specificity monoclonal antibody. With Salmonella Enteritidis ( S. Enteritidis) as a model target, this protocol can selectively detect 80 CFU mL −1 S. Enteritidis within 11 min in the optimized conditions. Moreover, with Listeria monocytogenes as another model target, the biosensor shows a high universality for detections of both gram-negative and gram-positive bacteria. The unexpected applicability of biological dye tracer in strip biosensor reveals that the biological dye can be a potential tool serving as a universal signal tracer for pathogenic microorganisms in food safety monitoring and early clinical diagnosis.
研究领域:
食品安全监测
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微生物学
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生物传感器技术
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临床诊断
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 15
·7070-7077
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IF 3.925
Zheng Lu,Wenxian Wei,Jingjing Yang,Qin Xu,Xiao-Ya Hu
A novel Ag triangle nanoparticles/TiO2 nanorod array (AgNTs/TNA) heterostructure was designed as a surface-enhanced Raman scattering (SERS) active substrate for the sensitive detection of Rhodamine 6G (R6G), Crystal Violet (CV), Malachite Green (MG) and Thiram in food samples. The heterostructure was constructed by simply stacking AgNTs and hydrothermally prepared rutile TNA together. On the surface of the heterostructure, the intensity of the Raman scattering intensity was about two times stronger compared with that on the AgNT substrate, using the R6G molecule as a probe. The AgNTs/TNA heterostructure could detect the SERS signal of R6G down to 10−10 M, showing excellent SERS performance. This platform has been successfully applied for the detection of CV and MG residues in crayfish. When irradiated with ultraviolet light, the SERS substrates can be cleaned and reused. This work demonstrated that the AgNTs/TNA metal–semiconductor heterostructure with high sensitivity, reproducibility and recyclability could provide an alternative reliable platform for food additive analysis.
研究领域:
材料科学
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分析化学
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食品安全
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DOI
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SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Jiao Zhou,Min Qing,Yu Ling,Lei Wang,Nian Bing Li,Hong Qun Luo
DNAzyme and G-quadruplex are purposed as universal recognition elements to achieve multi-functional signal amplification and readout, showing great potential in the design of biosensors and nanodevices. Herein, the single-stranded DNA (ssDNA) opens the dumbbell-shaped hairpin probes, the generated double-stranded DNA (dsDNA) is constructed as a nanobridge, and the released G-rich sequence is folded into a G-quadruplex village. The fluorescence of crystal violet/G-quadruplex complex villages is enhanced by the dsDNA nanobridge connections. Under the excitation wavelength of 580 nm, the fluorescence emission intensity at 624 nm exhibits a good linearity with the concentration of crystal violet (CV) in the range of 10–250 nM, which can be used for sensitive detection of CV . Additionally, combined with Pb 2+ -dependent DNAzyme, a platform for quantitative detection of Pb 2+ is established. In the concentration range of 5 nM – 25 μM, the fluorescence signal increases with the increase of Pb 2+ concentration. The quantitative detection limit of Pb 2+ by this method is 5 nM, and it possesses good selectivity and recovery for Pb 2+ in a variety of water samples. More importantly, the platform can sensitively detect various metal ions by changing the DNAzyme sequence. It has potential application in the field of environmental monitoring.
研究领域:
生物化学
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纳米技术
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分析化学
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环境科学
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DOI
高影响因子
Scientific Reports
·
2026
·
IF 4.9
Wang Jinghan,Hou Peng,Yao Qingwei,Fu Shuang,Chao Liyuan,Sui Huimin,Han Lu,Wang Jiafeng,Wang Guowei,Zhang Hongguang
Surface-enhanced Raman scattering (SERS) technology has emerged as a powerful analytical tool for trace-level environmental pollutant detection, with its performance critically dependent on the physicochemical properties of substrate materials. While noble metal substrates (e.g., Au/Ag) demonstrate superior SERS enhancement through surface plasmon resonance (SPR), their practical deployment is hampered by inherent limitations including high material costs and susceptibility to oxidation-induced degradation. Herein, we report the rational design of Ag 2 O/TiO 2 composite substrates via a facile wet-chemical precipitation method, wherein Ag 2 O nanoparticles are uniformly decorated onto pre-synthesized TiO 2 nanospheres. Upon application in crystal violet (CV) detection, the optimized Ag 2 O/TiO 2 composite substrate exhibits remarkable SERS performance with an enhancement factor (EF) of 6.7 × 10 6 and an ultra-low detection limit (LOD) of 1.0 nM (1.0 × 10⁻ 9 M). Systematic investigations reveal that the Ag 2 O modification induces photogenerated electron accumulation on the TiO 2 surface, promoting photoinduced charge transfer (PICT) between the substrate and adsorbed CV molecules. This work not only presents a cost-effective and stable SERS platform for environmental contaminant monitoring but also provides mechanistic insights into the rational engineering of semiconductor-based SERS substrates with enhanced sensitivity and durability.
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Nanoscale
·
2026
·
IF 5.2
Zhiqi Zhang,Jiazheng Lin,Zhuorui Wen,Kerui Hu,Lihui Zhang,Jinying Zhang
Violet phosphorus structure, a promising van der Waals semiconductor, has attracted growing attention due to its unique properties. It is still a big challenge to grow violet phosphorus or phosphorene on certain substrate. Herein, violet phosphorus flakes with tuneable sizes have been directly grown on silicon substrate using exfoliated violet phosphorene nanosheets as seeds. A chemical transport method based on P/I/Sn sources was adopted to epitaxial growth of violet phosphorus flakes. The edges of violet phosphorene nanosheets were found to decompose initially with increasing temperature, subsequently acting as homogeneous nuclei for the growth of violet phosphorus flakes in both the lateral and vertical (out-of-plane) directions. The size of violet phosphorus flakes was found to increase with increasing reaction time. The average lateral sizes of the as-grown violet phosphorus flakes were found to grow from ~0.98 μm to ~41.54 μm, with average thickness from ~0.15 μm to ~3.82 μm after 48 h of reaction. The as-grown flakes were further confirmed using a marked silicon substrate, where the violet phosphorene nanosheet seeds were found to grow from lateral size of 0.95/1.04 μm with thickness of 0.09/0.11 μm to 30.22/36.64 μm with thickness of 2.31/2.57 μm after 10 h of reaction. The successful growth of violet phosphorus flakes on silicon substrate enables the development of advanced electronic and optoelectronic devices based on violet phosphorus and phosphorene structures, enhancing the functionality of next-generation technologies.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xiaoyue Hui,Moustafa Zahran,Amal H. Marei,Ying Xie,Qiyu Liu,Guozhong Xu,Huizhong Chi,Xiaolin Li
Quantification of toxic dyes in polluted water is essential to environmental monitoring and control. Herein, a novel electrochemical sensor has been developed to quantify crystal violet (CV) using alginate-silver nanoparticles (Alg-AgNPs) fabricated by the direct chemical reduction technique. Alg- AgNPs synthesis was visually indicated, then experimentally confirmed employing an ultraviolet-visible spectrophotometer, which showed AgNP absorption peak at 400 nm, and square wave voltammetry (SWV), which exhibited the AgNP oxidation peak at 0.3 V. Additionally, X-ray diffraction analysis demonstrated the crystalline nature of Alg-AgNP, while transmission electron microscopy showed the spherical/semi-spherical shape of AgNPs with an average particle size of 42.9 nm. The prominent role of Alg-AgNPs in the electrocatalysis of CV oxidation was evidenced using cyclic voltammetry and SWV. AgNP-modified glassy carbon electrode sensor exhibited detection and quantification limits of 0.08 and 0.28 μM, respectively. Additionally, the sensor’s selectivity was demonstrated through interference studies and real-sample analysis.
研究领域:
环境科学
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纳米技术
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分析化学
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电化学
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水质监测
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Xiaoliang Qi,Qiankun Zeng,Xianqin Tong,Ting Su,Lei Xie,Kai Yuan,Jianxiong Xu,Jianliang Shen
Both secondary pollution and the low mechanical strength of adsorbents have severely impeded the practical application of adsorption methods in the dye wastewater treatment. In this work, we innovatively synthesized a composite hydrogel adsorbent by incorporating polydopamine (PDA) and montmorillonite (MMT) into the pullulan hydrogel matrix for dye adsorption. First, the successful formation of the resultant adsorbents was verified by Fourier-transform infrared spectroscopy and scanning electron microscope elemental mapping analysis. Then, several physicochemical properties (such as thermal and swelling properties, microarchitecture, and mechanical strength) of the five prepared adsorbents (PM1–PM5) were investigated. These results demonstrated the adsorbents had tunable properties, which can be achieved by adjusting the PDA/MMT mass ratio. Next, the dye adsorption performance was systematically explored. The resultant adsorbent (PM3) had a maximum adsorption capacity of 112.45 mg/g and its adsorption data was best described by a Langmuir isotherm and pseudo-second-order kinetic. Finally, the adsorption mechanisms and potential commercial practicability of the adsorbent were studied. Altogether, the designed adsorbent could effectively avoid generating the secondary pollution typical of adsorbents and it displayed excellent mechanical strength, thus opening up a new horizon in mitigating environmental pollution from textile industries.
研究领域:
环境科学
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材料科学
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化学工程
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KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2016
·Vol. 33
·期 9
·2659-2667
·
IF 1.408
Xu Wenjing,Zhang Wensheng,Li Yan,Li Wei
A new acrylic-lignosulfonate resin (ALR) was obtained by radical polymerization using calcium lignosulfonate (LS-Ca) and acrylic acid (AA) as raw materials. ALR was characterized using FT-IR, SEM, TG-DSC and N 2 adsorption–desorption surface area and pore size analyzer. Batch adsorption, including initial concentration, time, pH, dosage and temperature on the adsorption of crystal violet (CV) by ALR, was systematically studied. The ALR contained porous structure and the specific surface area of ALR was 190.55 m 2 /g with average pore diameter 11.34 nm. The kinetic and equilibrium data fitted into the pseudo-second-order model and Freundlich isotherm model, respectively. The adsorption process was slightly influenced in the range of pH=3–12. The maximum adsorption capacity of CV was found to be as high as 150.40±4.80mg/g for 24 h at 25 °C. Thermodynamic parameters were evaluated, and their values indicated that adsorption of CV on ALR was an exothermic process and spontaneous.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2022
·Vol. 101
·期 6
·3309-3321
·
IF 2.5
Jinjin Fan,Shuwen Xue,Keji Wan,Guoqiang Wang,Zhenyong Miao
Electrolysis technology is very effective in removing organic pollutants including dye wastewater and takes into account environmental friendliness. This paper deals with the electrochemical degradation of triphenylmethane dye crystal violet (DCV) from the aqueous solution using 304 stainless steel electrodes. The electrolytic cell device with a flotation column structure was set up and the electrolytic degradation process was studied. It could be found that the DCV removal efficiency and reaction rate constants were fitting the first-order kinetic model. The process performance was analyzed in terms of degradation efficiency and energy consumption. Almost complete degradation (>98%) of 100 mg/L DCV was achieved after the 40 min reaction under the conditions of 300 mg/L NaCl dosage, nature pH (5–7), and 10 V applied voltage. Finally, the behaviours of degradation were studied. In the process of electrochemical degradation, the synergistic effects of electrode electrolysis, including indirect oxidation of electrolyte, hydroxyl radical, flocculation, and air flotation, removed the dye pollutants from the wastewater. The present study has proved the effectiveness and cleanness of electrochemical treatment for the triphenylmethane dye solution.
研究领域:
环境科学
·
化学工程
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Journal of Water Process Engineering
·
2024
·
IF 6.3
Guanqun Tang,Huiyin Mo,Le Gao,Yidi Chen,Xu Zhou
The purpose of this study is to investigate the optimal preparation conditions for alkali-modified pomelo peel biochar and to clarify its adsorption process towards crystal violet (CV). The pyrolysis temperature and the alkali-to-carbon ratio of pomelo peel raw materials with varying epidermal layers were evaluated and optimized. Several characterization techniques demonstrated that alkaline modification enhances the adsorption performance of biochar by modifying its pore structure and functional group composition. Notably, the biochar, derived from the outer epidermis of pomelo, modified with an alkali-to-carbon ratio of 2:1 at 900 °C (KC2), exhibited significant adsorption capacity through mechanisms such as pore filling, hydrogen bonding, π-π interactions, electrostatic interactions, and functional groups including C C and C O C. The Freundlich isotherm and the pseudo-second-order kinetic model were determined to be the most appropriate for describing the equilibrium data. Accordingly, KC2 exhibited a maximum adsorption capacity of 805.69 mg/g, which significantly exceeded that of biochar derived from pomelo peel with a basic outer epidermis pyrolyzed at 900 °C (BC-GOP900, 586.7 mg/g). This study ultimately concluded that utilizing pomelo peel biochar, particularly KC2, represents an innovative strategy to address pollution caused by CV while effectively repurposing agricultural waste.
研究领域:
环境科学
·
材料科学
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DOI
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JOURNAL OF MOLECULAR LIQUIDS
·
2017
·
IF 3.648
Zhiyun Chen,Zilin Yang,Weiguo Shen
The reaction kinetics for alkaline fading of malachite green (MG) in the critical binary solution 2-butoxyethanol + water and of crystal violet (CV) in the critical microemulsion water/bis(2-ethylhexyl) sodium sulfosuccinate (AOT)/ n -decane with the molar ratio of water to AOT being 30 have been studied by the three-wavelength UV-spectrophotometry at initial reaction stage and various temperatures. It was found that the reaction rates k obs for both above reactions were well described by the Arrhenius equation in the temperature ranges far away from the critical points. The critical slowing down effects were observed in the near-critical regions and the corresponding critical slowing down exponents were determined to be 0.146 ± 0.014 and 0.035 ± 0.006 for the two reactions, respectively. The former exhibited both the dynamic and thermodynamic critical slowing down effects; while the latter showed no thermodynamic singularity. It was attributed to the particular experimental design in this work that made the dimer/monomer droplet equilibrium in the critical microemulsion system no longer affect the reaction kinetics.
研究领域:
化学动力学
·
物理化学
·
胶体与界面化学
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CHEMOSPHERE
·
2023
·
IF 8.8
Long Wang,Chunbao Zhou,Yanxin Yuan,Yajie Jin,Yang Liu,Zhihui Jiang,Xiangtong Li,Jianjun Dai,Yingwen Zhang,Asif Ali Siyal,Wenya Ao,Jie Fu,Junshen Qu
Metals-loaded (Fe 3+ , Cu 2+ and Zn 2+ ) activated carbons (M@AC) with different loading ratios (0.1%, 0.5%, 1%, 5% and 10%) were prepared and employed for catalytic degradation of dye model compounds (crystal violet (CV) and methyl orange (MO)) in wastewater by heterogeneous Fenton-like technique. Compared with Cu@AC and Zn@AC, 0.5% Fe 3+ loaded AC (0.5Fe@AC) had better catalytic activity for dyes degradation. The effects of dyes initial concentration, catalyst dosage, pH and hydrogen peroxide (H 2 O 2 ) volume on the catalytic degradation process were investigated. Cyclic performance, stability of 0.5Fe@AC and iron leaching were explored. Degradation kinetics were well fitted to the pseudo-second-order model (Langmuir-Hinshelwood). Almost complete decolorization (99.7%) of 400 mg L −1 CV was achieved after 30 min reaction under the conditions of CV volume (30 mL), catalyst dosage (0.05 g), H 2 O 2 volume (1 mL) and pH (7.7). Decolorization of MO reached 98.2% under the same conditions. The abilities of pyrolysis char (PC) of dyeing sludge (DS) and metal loaded carbon to remove dye pollutants were compared. The intermediate products were analyzed and the possible degradation pathway was proposed. This study provided an insight into catalytic degradation of triphenylmethane- and aromatic azo-based substances, and utilization of sludge char.
研究领域:
环境科学
·
材料科学
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高影响因子
危险品化学品经营许可证（带存储）