Herein, we report an effectual method for designing a novel form of nanozyme laccase mimic namely Cu/H3BTC, using copper ions and 1,3,5-benzene tricarboxylic acid (1,3,5-H3BTC). This Cu-based metal–organic framework (MOF) was synthesized through a simple procedure of mixing of two usual reagents at room temperature. Amido Black 10B (AB-10B) was chosen as a model dye for degradation consequences. Results showed that Cu/H3BTC MOF revealed significantly higher catalytic efficacy under certain conditions like high pH, extreme temperature and high salt conditions and it has long-term storage stability, which can lead to a significant decline in catalytic activity of laccase. In addition, the degradation of AB-10B was up to 60% after ten cycles, showing good recyclability of Cu/H3BTC MOF. The UV-visible spectral changes clearly showed that Cu/H3BTC MOF is an effective laccase mimic for the degradation of azo dye AB-10B, which was degraded more easily within the time duration of 60 min. The Cu/H3BTC MOF also possessed fundamental activities like laccase with regard to oxidation of the phenolic compounds. Moreover, a technique for the quantitative detection of epinephrine by Cu/H3BTC MOF was established. These findings help to understand the laccase-like reactivity and provide a basis for the future design and application of metal-based catalysts.
The objective of this study was to design a more effective antibacterial agent to overcome the problem of fast-growing bacterial resistance. The results showed that a Cu/H3BTC metal–organic framework (MOF) exhibited a notable antibacterial performance towards Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and could significantly disrupt the cell membrane, resulting in the discharge of cell constituents. In addition, morphological changes were elucidated using scanning electron microscopy (SEM). SEM photographs demonstrated noticeable and apparent changes in the morphology of bacterial cells, signifying cell damage that was further confirmed via confocal laser scanning microscopy (CLSM). Furthermore, the result of agarose gel electrophoresis (AGE) showed that Cu/H3BTC MOF could enter the bacterial cells by impairing cell membranes and inhibiting the synthesis of DNA. These findings indicated that the Cu/H3BTC MOF is a potent antimicrobial material that can be applied to hinder the growth of pathogenic microbes.
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