Cu/N co-doped TiO 2 nanopowder was prepared with a facile sol–gel method. In the absence of stabilizing agents, the co-doped nanopowder with nanoscaled particle sizes was obtained. Doping didn’t change the anatase crystal structure of TiO 2 . Cuprite Cu 2 Cl(OH) 3 formed in the co-doped nanopowder. Based on ultraviolet–visible (UV–vis) spectroscopy and X-ray photoelectron spectroscopy (XPS) data, the mechanisms of significantly improved photocatalytic activity of the co-doped nanopowder were discussed. The antibacterial evaluation and cytotoxicity test suggested the nanopowder inhibited bacteria significantly under visible light and had ultralow cytotoxicity. This work provided a promising antibacterial agent with potential biomedical applications.
Graphical abstract
Xiaobin Zhou,Mengjun Wang,Junyang Chen,Xingguang Su
Developing high-performance nanozyme for the construction of high-sensitivity biosensor platform is of great significance. Herein, we have designed and synthesized a Cu/N co-doped carbon-based nanozyme by anchoring Cu atoms on two-dimensional carbon nanosheet (Cu/NC NS). The Cu/NC NS possessed ultra-high Cu loading, superior peroxidase-like catalytic activity, great stability and reusability. Significantly, the Cu/NC NS exhibited high affinity towards H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB) under acidic condition, which could induce obvious color changes of TMB in the presence of low H 2 O 2 concentration. By integrating the Cu/NC NS with β-galactosidase (β-Gal) and galactose oxidase (Gal Ox), a multienzyme cascade colorimetric sensing system was established for the detection of lactose and β-Gal, which realizing the assay of lactose and β-Gal in the linear ranges of 0.1–1.4 mM and 0.025–0.2 U/mL, respectively. And the limit of detection for lactose and β-Gal were 0.03 mM and 0.01 U/mL, respectively. Furthermore, this work achieved the accurate detection of the content of lactose in milk and the activity of β-Gal in human serum, exploiting a novel application for nanozyme in biosensing.
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