MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-10
·
IF 5.3
Liu Menglong,Wang Ying,Xiao Jingyi,Liu Yiyao,Ren Yi,Gao Xue
Based on the peroxidase activity of Cu-hemin metal–organic framework (Cu-hemin MOF) nanozyme, a colorimetric enzyme-linked immunosensor was developed for the detection of furazolidone (FZD). Cu-hemin MOF is a bimetallic nanozyme that exhibited a stronger catalytic effect compared with single-metal organic framework nanoenzymes. Cu-hemin-MOF catalyzes hydrogen peroxide (H 2 O 2 ) to produce hydroxyl radicals (•OH), which oxidizes the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB). The absorbance change is at 650 nm. The content of AOZ in animal food can be quickly and accurately determined by changes in absorbance. The linear range of the colorimetric biosensor for detecting FZD was 0.01 ~ 62.52 ng/mL, and the limit of detection was as low as 0.01 ng/mL. The recovery of spikes samples was in the range 94.2-108.0 % and reproducibility was less than 4.8%. In addition, the cross-reaction rate was less than 0.1% when detecting other metabolites except AOZ, indicating that the sensor has good applicability and specificity. This study not only provides a better understanding of the relationship between the dispersion of nanoenzymes and enzyme-like activity but also offers a general method for detecting antibiotics using the nanoenzyme colorimetric method.
研究领域:
食品安全检测
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2023
·
IF 3
Zitong Wang,Haotian Wu,Yuan Fang,Hao Yang,Xiaomin Wang
The increasing drug resistance of bacteria due to the inappropriate use of various antibiotics has become an ongoing public health problem around the world. In this work, a novel Cu-hemin nanosheet with multilayer structure was synthesized by a simple green method. Integrating the peroxidase properties of hemin with the excellent photothermal conversion performance of IR780, the as-prepared Cu-hemin@IR780 can not only effectively catalyze hydrogen peroxide to produce reactive oxygen species (ROS), but also has a highly efficient and stable photothermal effect for sterilization. The antibacterial efficiency against MRSA can reach up to 98.96% by using chemodynamic therapy (CDT) and photothermal therapy (PTT) for synergistic sterilization.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
Chen Xiaolong,Wang Xianfeng,Cao Gaihua,Wu Yawen,Luo Huibo,Ji Zhong,Shen Caihong,Huo Danqun,Hou Changjun
Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H 2 O 2 . DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Graphical abstract
研究领域:
材料科学
·
分析化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Xiying Chen,Haijuan Qin,Xiao Zhou,Weiran Li,Jingjing Zhang,Shuo Wang,Yaqing Liu
Azodicarbonamide (ADA) in flour products is easily converted to semicarbazide which greatly threatens human health. Herein, a long-lasting chemiluminescence (CL)-based biosensor was developed for quantitative point-of-care testing (POCT) of ADA. The threonine (Thr)-functionalized Cu-hemin MOFs (Cu-hemin@Thr) could induce persistent CL of luminol with excellent stability. The CL intensity was related to the competition reaction among ADA and a composite of glutathione-silver ions (GSH-Ag + ). In the presence of ADA, GSH is oxidized to glutathione disulfide (GSSG), which breaks the coordination between Ag + and GSH. The CL of the sensing system is then decreased which is expected to be used for ADA detection. By combining a homemade portable device as a detector and a smartphone as an analyzer, quantitative POCT of ADA was successfully achieved. The limit of detection was 0.562 μM (0.065 ppm), which is much lower than the maximum permissible concentration of ADA (45 ppm) in flour extract. The developed strategy demonstrated quantitative POCT capabilities along with advantages of low cost, excellent selectivity, and repeatability, presenting great potential application in food safety and environment monitoring.
研究领域:
分析化学
·
食品安全
·
环境监测
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）