MATERIALS LETTERS
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2021
·
IF 3.423
Chunyun Ma,Yan Zhang,Chuankai Yang,Miaorong Zhang,Jianguo Tang
Lipase was immobilized with Cu 3 (PO 4 ) 2 ⋅3H 2 O to synthesize lipase-Cu 3 (PO 4 ) 2 ⋅3H 2 O hybrid microflowers (Lip-MFs). Surfactants (Tween 80, Triton X-100 and CTAB) were added to study their effect on the morphology and activity of Lip-MFs. SEM images revealed that surfactants can increase the size of Lip-MFs and make the hierarchical structure more compact. Further, compared with other surfactants, the activity of CTAB-Lip-MFs was 1.45 times of that of free lipase, demonstrating CTAB is conducive to improve the activity of lipase.
研究领域:
生物化学
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材料科学
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Min Lu,Hao Zhang,Xu Wang,Hui Jiang,Guang Hu,Feng-Qing Yang
A simple, convenient and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower was developed. The structural properties of the materials were studied by several characterization techniques. Subsequently, the enzymatic reaction conditions such as the pH value and temperature were optimized, and the enzyme kinetics and inhibition parameters were determined. The PA modified α-Glu/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower had better enzymatic activity under a wide pH range and high temperature than the free one. After seven successive cycles, the PA modified α-Glu/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower could still maintain approximately 63.0 % of its initial immobilized enzyme activity. The Michaelis-Menten constant ( K m ) and the half-maximal inhibitory concentration (IC 50 ) of acarbose were determined as 0.77 mM and 15.01 μM, respectively. In addition, the material was applied to evaluate the inhibitory activity of ten phenolic compounds on α-Glu, and epicatechin gallate, gallocatechin gallate, epigallocatechin gallate and rosmarinic acid showed good inhibitory activity with % of inhibition of (53.42 ± 2.39)%, (37.28 ± 1.32)%, (37.08 ± 0.63)% and (35.53 ± 0.23)%, respectively. These results indicate that the PA modified hybrid nanoflower is an efficient method of α-Glu immobilization.
研究领域:
生物化学
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纳米技术
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酶工程
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药学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2021
·
IF 3.978
Miaorong Zhang,Yan Zhang,Chuankai Yang,Chunyun Ma,Jianguo Tang
A novel smartphone-based colorimetric biosensor based on enzyme-inorganic hybrid nanoflowers was applied for ultrasensitive determination of of hydrogen peroxide (H 2 O 2 ). Horseradish peroxidase-inorganic hybrid nanoflowers (HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs) were facilely synthesized by a one-pot incubation using HRP and Cu 3 (PO 4 ) 2 ⋅3H 2 O as organic and inorganic components, respectively. Characterization results revealed HRP molecules were well immobilized in HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs via self-assembly. The results of smartphone-based colorimetric assay successfully demonstrated the good sensing performance of HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs. The linear detection range of H 2 O 2 was from 5 to 500 μM, and the detection limit was 0.5 μM. Further, the proposed assay showed good anti-interference ability and high sensitivity for H 2 O 2 detection owing to the high specificity of HRP and excellent accuracy of color parameters obtained by Color Detector software, respectively. In addition, HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs had desirable storage stability and reusability, and this colorimetric system can realize the H 2 O 2 detection in tap water, which indicated the good applicability of HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs in real sample.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
RSC Advances
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2015
·Vol. 5
·期 117
·96997-97002
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IF 3.84
Liwen Liang,Xu Fei,Yue Li,Jing Tian,Longquan Xu,Xiuying Wang,Yi Wang
We have synthesized a novel composite material with a hierarchical flower-like structure and extremely high enzyme activity. The spherical, hierarchical flower-like structure with numerous small flowers was formed through self-assembly using papain as the organic component and Cu3(PO4)2·3H2O as the inorganic component. This hybrid-nanoflower structure was confirmed by Fourier transform infrared spectroscopy, and X-ray diffraction. The enzyme activity of papain embedded in the hybrid nanoflowers was further evaluated using BAEE as a substrate. Compared with free papain in solution, the hybrid materials exhibited extremely high enzyme activity (∼4510%). A study on the relationship of enzyme weight percentage and pattern structure with enzyme activity revealed that enzyme activity was mainly affected by the material structure. These results demonstrate that this hierarchical structure can effectively increase enzyme activity.
研究领域:
材料科学
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生物化学
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纳米技术
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DOI
高影响因子
FOOD CHEMISTRY
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2019
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IF 5.399
Nuan Feng,Haiyang Zhang,Yao Li,Yangkaixi Liu,Longquan Xu,Yi Wang,Xu Fei,Jing Tian
Milk allergenic proteins are the main reason of cow’s milk allergy. The most common method for removing milk allergenic proteins is enzymatic hydrolysis . However, the direct application of protease to hydrolyze allergens will lead to the introduction of new allergenic proteins. An ideal strategy for removing milk allergenic proteins without introducing new allergenic proteins is immobilization of protease. Herein, we established a simple method to synthesize a novel papain-Cu 3 (PO 4 ) 2 ·3H 2 O–magnetic nanoflowers (PCMNs). The PCMNs demonstrated 1556% higher activity than the free alkaline papain . Moreover, the PCMNs could hydrolyze most of allergenic proteins in cow’s milk, generating low-sensitive milk. In cycle analysis, the PCMNs also exhibited good reusability and were easily separated from the product.
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Hua-Wei Zhang,Ke Cheng,Jing-Jing Tang,Ling-Zhi Liu,Ruo-Xuan Qin,Yi-Nuo Sun,Jiang-Hua Zhang,Da-Yong Zhou
To synthesize structured phospholipids (SPLs) in an efficient and green manner is of great significance. Herein, novel Fe 3 O 4 @SiO 2 - Aspergillus oryzae lipase (AOL)/copper phosphate trihydrate nanoflowers (FLCNs) were prepared by coprecipitation method and then characterized by energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer etc. FLCN activity was further evaluated by promoting functional medium-chain SPL (MCSPL) or short-chain SPL (SCSPL) synthesis via biotransesterification between soybean lecithins and medium-chain fatty acids (MCFAs) or short-chain fatty acids (SCFAs) under optimal reaction conditions. Moreover, FLCNs and free AOLs were comparatively assayed in enzymatic properties. As a result, FLCNs were successfully fabricated with sufficiently large surfaces and numerous enzyme active sites. Owing to these structural features, after reacting at 40 °C for 36 h with FLCN dosage of 6% and substrate mass ratio of 1:3:3, FLCNs exhibited much more excellent activity in MCSPL synthesis with 88.30 ± 0.63% of MCFA incorporation than benchmark enzymes and heterogeneous functional materials. Most significantly, in the first biosynthesis of functional SCSPLs facilitated by FLCNs, much superior SCFA incorporation of 66.55 ± 0.73% was obtained. Notably, FLCNs also possessed convenient magnetic separability together with superior pH and temperature tolerances, thermal and storage stabilities, and recyclability to free AOLs. In brief, the present investigation provides a new biological strategy for effective, economic, convenient, and eco-friendly SPL synthesis in food and pharmaceutical industries, highlighting its merits in functional lipid production.
研究领域:
食品科学
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生物工程
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材料科学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Chunyun Ma,Yan Zhang,Chuankai Yang,Yuhang Zhang,Miaorong Zhang,Jianguo Tang
Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) were fabricated via biomineralization using Cu 3 (PO 4 ) 2 ⋅3H 2 O as the mineral for olive oil hydrolysis. FT-IR spectra indicated that immobilized lipase molecules in CTAB-Lip-MHFs has good structural integrity, and XPS spectra revealed that immobilized lipase molecules were present at the surface of CTAB-Lip-MHFs. Kinetic studies suggested that CTAB-Lip-MHFs had good substrate affinity ascribed to interfacial effect caused by CTAB, meanwhile, the open conformation of lipase activated by CTAB contributed to increased reaction rate with substrate. The generated fatty acids via hydrolysis of olive oil by CTAB-Lip-MHFs were 146% of that by free lipase, moreover, CTAB-Lip-MHFs could retain 76.5% and 93.2% of initial activities after storage at 50 °C for 10 h and 4 °C for 30 days, respectively, indicating the good stability of CTAB-Lip-MHFs. In addition, after 5 recycles, CTAB-Lip-MHFs preserved 72.7% of initial activity, demonstrating the favorable reusability of CTAB-Lip-MHFs for fatty acid production.
研究领域:
生物化学
·
酶工程
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产品关联
DOI
高影响因子
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