LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Lin Wang,Feng Li,Sufang Wang,Jinsong Wu,Wenhui Zhang,Yan Zhang,Wentao Liu
The application of enzyme-inorganic hybrid nanoflowers in time-temperature indicators (TTIs) was studied for the first time. Lipase@Cu 3 (PO 4 ) 2 hybrid nanoflowers were prepared by the interaction between lipase and copper ions. Compared with free lipase, the activity of lipase in the nanoflowers was increased by 137%, and the storage stability was also improved significantly. Based on the enzymatic reaction between lipase and glycerol triacetate, six TTI prototypes with different concentrations of lipase@Cu 3 (PO 4 ) 2 hybrid nanoflowers were investigated. The color of the six TTIs changed from purple to yellow, and finally to green, and the activation energies were 46.86 kJ/mol, 51.17 kJ/mol, 54.99 kJ/mol, 60.00 kJ/mol, 62.17 kJ/mol, and 65.63 kJ/mol, respectively. The shelf life of TTIs can be determined by changing the ratio of lipase@Cu 3 (PO 4 ) 2 hybrid nanoflowers to glycerol triacetate, therefore the TTIs can be easily applied to real-time safety evaluation of many perishable foods.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
MATERIALS LETTERS
·
2021
·
IF 3.423
Chunyun Ma,Yan Zhang,Chuankai Yang,Miaorong Zhang,Jianguo Tang
Lipase was immobilized with Cu 3 (PO 4 ) 2 ⋅3H 2 O to synthesize lipase-Cu 3 (PO 4 ) 2 ⋅3H 2 O hybrid microflowers (Lip-MFs). Surfactants (Tween 80, Triton X-100 and CTAB) were added to study their effect on the morphology and activity of Lip-MFs. SEM images revealed that surfactants can increase the size of Lip-MFs and make the hierarchical structure more compact. Further, compared with other surfactants, the activity of CTAB-Lip-MFs was 1.45 times of that of free lipase, demonstrating CTAB is conducive to improve the activity of lipase.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 14
·4139-4147
·
IF 4.478
Ye Cui,Xu Songmin,Wu Zhen,Wang Minqiang
Propyl gallate (PG) as one of the most important additives has been widely used to prevent or slow the oxidation of foods in the food industry. In this work, Cu 3 (PO 4 ) 2 /BiVO 4 composite is synthesized through two hydrothermal processes. With visible light irradiation, the Cu 3 (PO 4 ) 2 /BiVO 4 composites modified PEC platform displays a superior anode photocurrent signal. The PEC sensor showed a wide linear range from 1 × 10 −10 to 1 × 10 −3 mol L −1 with a detection limit as low as 0.05 × 10 −10 mol L −1 . The Cu 3 (PO 4 ) 2 /BiVO 4 photoelectrochemical (PEC) sensor is designed and characterized by electrochemical impedance. Compared with GCE/BiVO 4 and GCE/Cu 3 (PO 4 ) 2 , the GCE/Cu 3 (PO 4 ) 2 /BiVO 4 has a higher photocurrent response. In addition, the sensor is highly selective for samples containing other antioxidants. Furthermore, the sensor can be used to detect PG in edible oil samples with satisfactory results. The recoveries of propyl gallate in edible oil ranged from 95.5 to 101.8%. The results show that Cu 3 (PO 4 ) 2 /BiVO 4 composites can be used to analyze PG in different edible oil samples, which are beneficial for food quality monitoring and reduce the risk of PG overuse in food. Graphical abstract
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2022
·
IF 18.027
Yuyue Mo,Feng Zhang,Hefeng Dong,Xingzhen Zhang,Shoujian Gao,Shenxiang Zhang,Jian Jin
Membrane fouling is a persistent and crippling challenge for oily wastewater treatment due to the high susceptibility of membranes to contamination. A feasible strategy is to design a robust and stable hydration layer on the membrane surface to prevent contaminates. A hydrogel illustrates a distinct category of materials with outstanding antifouling performance but is limited by its weak mechanical property. In this research, we report a reinforced hydrogel on a membrane by in situ growing ultrasmall hydrophilic Cu3(PO4)2 nanoparticles in a copper alginate (CuAlg) layer via metal-ion-coordination-mediated mineralization. The embeddedness of hydrophilic Cu3(PO4)2 nanoparticle with a size of 3–5 nm endows the CuAlg/Cu3(PO4)2 composite hydrogel with enhanced mechanical property as well as reinforced hydrate ability. The as-prepared CuAlg/Cu3(PO4)2 modified membrane exhibits a superior oil-repulsive property and achieves a nearly zero flux decline for separating surfactant stabilized oil-in-water emulsions with a high permeate flux up to ∼1330 L m–2 h–1 bar–1. Notably, it is capable of keeping similar permeate flux for both pure water and oil-in-water emulsions during filtration, which is superior to the currently reported membranes, indicating its super-antifouling properties.
研究领域:
环境工程
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材料科学
·
化学工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Mengying Luo,Yinnan Song,Niaz Ali Khan,Wanru Wei,Ying Lu,Qinghua Zhao,Haiqing Jiang,Mufang Li,Dong Wang
H 2 O 2 sensor is widely used in biomedicine, environmental governance and food processing. However, it is limited by the complex preparation processes and high cost of raw materials. In this work, we develop a facile, simple and cost-effective method to fabricate H 2 O 2 sensor based on a screen-printed electrode via in-situ growth method. The copper was first electrodeposited on the working electrode of screen-printed electrode (SPE) as a sacrificial layer, subsequently, the SPE was immersed in horseradish peroxidase (HRP) containing phosphate buffer saline (PBS). The Cu ions formed by oxidation of the copper layer combined with HRP and phosphate ions to construct HRP-Cu 3 (PO 4 ) 2 hybrid nanoflowers on its surface. The fabrication process is straightforward, without complex reaction conditions and dangerous drugs, which is conducive to mass production. This sensor showed a good linear response to H 2 O 2 with a detection limit of 1.8 μM and a sensitivity of 263.3 μA∙mM −1 ∙cm −2 . Additionally, it possessed excellent selectivity and long-time stability. The performance of this sensor was investigated in various beverages such as milk, beer, and juice, showing reliable detection of H 2 O 2 which highlights the potential practical applications of the sensor.
研究领域:
生物医学工程
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分析化学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 9
·1687-1698
·
IF 4.3
Liu Cheng,Zheng Jingtian,Zhang Benxing,Zhong Xianhua,Wang Wei,Li Zhangyong
The Fenton reaction is one of the most effective methods for treating organic wastewater, which is extremely harmful to humans but difficult to treat. However, finding simple, low-cost, and efficient catalysts for the Fenton reaction remains a challenge. In this study, a BSA-Cu 3 (PO 4 ) 2 hybrid nanoflower (NF) was synthesized to investigate its peroxidase-like activity for the treatment of organic wastewater. Its morphology, composition, and crystallization had been fully studied and the results confirmed that the NFs were successfully prepared. Subsequently, the origin of the peroxidase-like activity of the NFs was further analyzed, with the results suggesting two reasons: (i) the transformation between Cu(I) and Cu(II) and (ii) nano-effects. Additionally, Congo red was selected as the organic pollutant to simulate the decolorization of wastewater. After 3 h, the decolorization efficiency reached 96%. Furthermore, the NFs exhibited good storage performance, maintaining approximately 90% relative activity after storage for 30 days. In summary, the NFs have great application prospects in the treatment of organic wastewater. Graphical abstract
研究领域:
环境科学
·
纳米技术
·
催化
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jingling Gong,Bin Xiang,Ruirui Jin,Zhaoyang Li,Weimin Liu,Jian Li
Membrane fouling is a key challenge to the sustainable operation of oil–water separation membranes. Constructing a stable hydration layer on the membrane surface as an oil-fouling resistance barrier by modulating the physicochemical structure of the membrane is one of the most effective strategies. Herein, inspired by the petal structure in nature, Cu 3 (PO 4 ) 2 and UiO-66-NH 2 particles were combined by a self-assembly strategy to construct CUP (Cu 3 (PO 4 ) 2 /UiO-66-NH 2 /PVA) composite membranes with anti-fouling capability against viscous crude oil. Benefiting from the petal structure and abundant polar groups within the membrane, the as-prepared membrane could easily combine with water molecules to form a stable hydration layer, which acted as a barrier to hinder the oil contamination of the membrane. Further, the hierarchical flower-like structure was conducive to the improvement of the emulsion breaking rate and permeation flux of oil-in-water emulsions. After 60 min continuous cross-flow separation, the obtained CUP composite membrane still remained prominent separation efficiency (99.6 %) and permeate flux (4685.96 LMH/bar). In addition, the CUP composite membranes showed outstanding resistance to acid and alkali as well as abrasion. This study provides an effective method for the advancement of high-performance membranes with high flux and anti-fouling capability in the flied of oily wastewater treatment.
研究领域:
膜科学
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材料工程
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环境工程
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分离技术
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Jian Rong,Tao Zhang,Fengxian Qiu,Yao Zhu
Issues with separation and recycling of suspension powder samples in wastewater hinder the practical application of hybrid enzyme materials for environmental pollution control. In this work, for the first time, laccase–Cu3(PO4)2 hybrid microspheres with hierarchical structure were successfully prepared and loaded on a treated copper foil surface. First, Cu8(PO3OH)2(PO4)4·7H2O nanoflowers (CPN) were synthesized on the copper foil surface by a solution-growth method. Then the laccase–Cu3(PO4)2 hybrid microspheres were loaded on the CPN surface via an immersion reaction method using a laccase-containing phosphate buffer solution (PBS) solution. The formation mechanisms of CPN and La–CPN (laccase–Cu3(PO4)2 hybrid microspheres on the CPN surface) are discussed in detail and mainly contain the following processes: crystal growth, coordination effect, in situ growth, and self-assembly. Compared with free laccase, the as-obtained La–CPN has a higher decoloration efficiency (more than 95%) and decoloration rate (nearly 3.6 times higher than that of free laccase) on Congo red dye (CR) solution in the short time of 3 h. Cyclic voltammetry results demonstrated that the oxidizability of immobilized laccase could be enhanced as a result of the presence of Cu2+. Meanwhile, the utilization of the CPN carrier and the unique nanostructure gave the laccase–Cu3(PO4)2 hybrid microspheres high and stable decoloration efficiency and improved the tolerance toward pH and temperature changes. La–CPN still maintained about 85% relative activity after storage for 10 days. The concept presented herein can be further expanded to the preparation of other hybrid enzyme materials for environmental control, medical treatment, and more applications.
研究领域:
环境科学
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材料科学
·
生物技术
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产品关联
DOI
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Xu Xu,Xiang Li,Guibin Liu,Xixi Wei,Daming Feng,Lei Zhang
In recent years, efficient oil–water separation has gradually become an indispensable part of environmental treatment. Superhydrophobic/superoleophilic materials with excellent self-cleaning performance are urgently required and remain challenging in the investigation of practical, rapid, and efficient separation of oil–water mixture and emulsion, especially those with robust surfaces that can be used in harsh conditions. In this work, a novel superhydrophobic/superoleophilic material was first fabricated by in situ constructing PDMS@ZIF-7/Cu3(PO4)2 hierarchical architectures on a copper mesh, which was adopted as a high flux and efficient separation material for gravity-driven separation of oil–water mixture as well as emulsion. The introduction of crucial Cu3(PO4)2 nanosheet interlayers created the ideal hierarchical structures and serve as partial templates for the subsequent in situ growth of hydrophobic ZIF-7 nanosheets. An improved superhydrophobicity (CA = 155°), permeation flux (102,000 L m–2 h–1), and preferred self-cleaning property were thus achieved by such manipulation of the copper mesh. The PDMS@ZIF-7/Cu3(PO4)2 mesh exhibited exceptional separation efficiency for diverse oil–water mixtures and emulsions attributed to the superhydrophobicity and the demulsification ability and considerable stability to cope with extreme environments including sunlight resistance, low temperature, and corrosion resistance, which prompted its promising applicability in cleaning emulsified wastewater.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Min Lu,Hao Zhang,Xu Wang,Hui Jiang,Guang Hu,Feng-Qing Yang
A simple, convenient and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower was developed. The structural properties of the materials were studied by several characterization techniques. Subsequently, the enzymatic reaction conditions such as the pH value and temperature were optimized, and the enzyme kinetics and inhibition parameters were determined. The PA modified α-Glu/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower had better enzymatic activity under a wide pH range and high temperature than the free one. After seven successive cycles, the PA modified α-Glu/Cu 3 (PO 4 ) 2 ·3H 2 O hybrid nanoflower could still maintain approximately 63.0 % of its initial immobilized enzyme activity. The Michaelis-Menten constant ( K m ) and the half-maximal inhibitory concentration (IC 50 ) of acarbose were determined as 0.77 mM and 15.01 μM, respectively. In addition, the material was applied to evaluate the inhibitory activity of ten phenolic compounds on α-Glu, and epicatechin gallate, gallocatechin gallate, epigallocatechin gallate and rosmarinic acid showed good inhibitory activity with % of inhibition of (53.42 ± 2.39)%, (37.28 ± 1.32)%, (37.08 ± 0.63)% and (35.53 ± 0.23)%, respectively. These results indicate that the PA modified hybrid nanoflower is an efficient method of α-Glu immobilization.
研究领域:
生物化学
·
纳米技术
·
酶工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-10
·
IF 5.7
Wang Na,Yang Juan,Luo Zhi,Qin Dongmiao,Wu Yusheng,Deng Biyang
Copper phosphate hybrid nanoflowers (Cu 3 (PO 4 ) 2 HNFs) were demonstrated to produce cathodic ECL emission in the presence of potassium persulfate (K 2 S 2 O 8 ) and then used as a carrier due to their large specific surface area. AgNPs modified on Cu 3 (PO 4 ) 2 HNFs provided more binding sites for immobilizing secondary antibodies and accelerating the electron transfer rate to enhance the ECL signal. In addition, FONDs-Au was used to capture primary antibodies due to its good biocompatibility and large specific surface area. A sandwich electrochemiluminescence (ECL) immunosensor based on copper phosphate hybrid nanoflower/Ag nanoparticle (Cu 3 (PO 4 ) 2 HNFs@Ag) composite and Au NPs-functionalized Fe 2 O 3 nanodendrites (FONDs-Au) was constructed to detect prostate-specific antigen (PSA) in real samples. Under optimal conditions, the constructed sandwich ECL immunosensor was sensitive to PSA with a detection limit of 0.037 pg/mL ( S / N = 3), a linear detection concentration range of 0.0001–50 ng/mL, and a recovery range of 97.33–102.5%. This immunosensor is expected to provide a method to detect PSA or other biomarkers. Graphical Abstract
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 5
·2357-2364
·
IF 2.936
Jianyun Yu,Xinxin Chen,Min Jiang,Anming Wang,Linlin Yang,Xiaolin Pei,Pengfei Zhang,Stephen Gang Wu
To develop an efficient and green immobilized biocatalyst for promiscuous catalysis which has a broad scope of applications, hybrid nanoflower (hNF) confined papain as a biocatalyst has been proposed and characterized in this study. hNFs were firstly prepared through mixing CuSO4 aqueous solution with papain in phosphate saline (PBS) at room temperature. The resulting hNFs were characterized by SEM and verified through a hydrolysis reaction with N-benzoyl-DL-arginine amide as substrate. Under optimal conditions, this nano-biocatalyst demonstrated a 15-fold hydrolytic activity compared with papain of free form, along with better thermal stability. A series of reaction factors (reaction temperature, time, and solvent) have been investigated for Knoevenagel condensation reactions with hNFs as catalyst. At optimal conditions, product yield of the hNFs catalyzed reaction was 1.3 fold higher than that of the free enzyme with benzaldehyde and acetylacetone as substrates. A few aldehydes and methylene compounds have also been used to test the generality and scope of this new enzymatic promiscuity. To sum up, the obtained hNFs demonstrate better catalytic properties than free papain and the inorganic metal-salt crystal can function as both support and promotor in biocatalysis.
研究领域:
生物化学
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材料科学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）