The excellent peroxidase-like activity of uniform CuCo2O4 microspheres with oxygen vacancy for fast sensing of hydrogen peroxide and ascorbic acid
High catalytic activity is essential for constructing a rapid and facile colorimetric sensor to determine H2O2 and interrelated biomolecules. CuCo2O4 microspheres were successfully prepared using a facile hydrothermal and pyrolysis method. The excellent peroxidase-like performance of CuCo2O4 microspheres was evaluated by employing a chromogenic substrate, colorless 3,3′,5,5′-tetramethylbenzidine (TMB), which can be catalytically oxidized by H2O2 into a blue ox-TMB product within only 1 min. Fluorescence results reveal that the catalytic activity of CuCo2O4 is attributed to the increasing amount of ˙OH from the decomposition of H2O2 and theoretical simulations by Materials Studio proved that the existence of a large number of oxygen vacancies effectively enhanced the catalytic performance. Based on the high catalytic activity of CuCo2O4 microspheres, a rapid colorimetric sensor with high selectivity has been designed for detecting H2O2 and ascorbic acid. Under optimal reaction conditions, the linear response ranges of H2O2 and AA are 1.00–10.00 mM and 1.00–10.00 μM. The detection limits of H2O2 and AA are 132.70 μM and 0.57 μM, respectively.
危险品化学品经营许可证（带存储）