Progress in Natural Science-Materials International
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2023
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IF 4.7
Haiyan Kang,Hongchao Geng,Sisi Shi,Zhongxian Song,Yuting Dong,Xiaole Yan,Chaohai Wang,Yanli Mao,Qingyun Cai
High concentrations of Cu 2+ exceeding what the required level for the cellular process can changes the properties of protein, affects the normal function of the body, but also affect the body's metabolism, so that the body's immunity appears to decline. Detection of Cu 2+ is important for the environmental monitoring. Here, a fluorescence probe that uses heavy metal-free and properties-superior CuInS 2 /ZnS was proposed for detection of Cu 2+ . The fluorescence intensity of CuInS 2 /ZnS quantum dots (QDs) decreased obviously after Cu 2+ were added, and the intensity with the concentration of Cu 2+ showed excellent linear relation within the scope of 0–50 μM, the LOD down to 0.45 μM, a possible detection mechanism was analyzed. The QDs was further applied to the detection of Cu 2+ in drinking water, manifesting the foreground of the current strategy.
研究领域:
生物化学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
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2019
·Vol. 7
·期 17
·2835-2844
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IF 5.047
Hui Zhang,Youshen Wu,Zhenhai Gan,Yuexuan Yang,Yiming Liu,Peng Tang,Daocheng Wu
Accurate and sensitive temperature sensing is of great importance for studying biological activities in cells and tissues. Due to the complexity of the microenvironments, most of the existing thermometry systems cannot meet the needs of accurate intracellular and in vivo temperature sensing with high-sensitivity and low-toxicity. After investigation, we found that the fluorescence intensity of CuInS2/ZnS quantum dots (QDs) is significantly temperature dependent, and these QDs can be used for intracellular and in vivo temperature sensing after being encapsulated by amphiphilic micelles (QD-micelles). These QD-micelles have a size of approximately 10.1 ± 2.5 nm and an emission band of approximately 650 nm with intense photoluminescence (PL). Meanwhile, its high thermal sensitivity of 2% °C−1 at near-room temperature is insensitive to solution pH, ionic strength, and protein concentration. Even at a concentration of 300 μg mL−1, our QD-micelles do not exert cytotoxic effects on HeLa and PC-3 cells. Similar to being dispersed in water, the QD-micelles continue to exhibit a high thermal sensitivity of 2.0% °C−1 intracellular and 2.1% °C−1 in tumor tissues, and the PL intensity (I) and temperature (T) over the physiological temperature range have a good linear correlation. Therefore, they have considerable potential in biomedical applications.
研究领域:
生物医学工程
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纳米医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Materials Research Express
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2019
·Vol. 6
·期 12
·125018
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IF 1.449
Hui Zou,Yi Wu,Caixia Yao,Shiqi Duan,Zhiyuan Liu,Zhengyu Yan,Shenghua Liao
A novel method for preparing CuInS2/ZnS quantum dots (CuInS2/ZnS QDs) with the crystal structure of sphalerite by one-pot hot injection was developed in this paper. The as-prepared core CuInS2 quantum dots (CuInS2 QDs) was uniform spherical nanoparticle with the average diameter of about 2 nm with the fluorescent quantum yields (QY) of about 2.6%. By capping a shell of ZnS on the surface of CuInS2 QDs, the formed CuInS2/ZnS QDs exhibited obvious enhanced fluorescent emission with the QY of about 20%. Intriguingly, the crystal structure of obtained CuInS2/ZnS QDs is sphalerite, which was confirmed by XRD. Considering the different terminal group between these small organic molecules, mercaptoacetic acid (TGA), thioglycerol (TGC) and mercaptoethylamine (MEA) were further selected as capping molecules to exchange the surface ligands of CuInS2/ZnS QDs. The obtained CuInS2/ZnS QDs modified with TGC exhibited excellent photostability in physiological conditions. To investigate the effect of ligand molecules on the biocompatible of CuInS2/ZnS QDs, the experiment on the cytotoxicity of CuInS2/ZnS QDs to A549 cells were conducted. The results indicated that CuInS2/ZnS QDs capped with TGC showed the lowest cytotoxicity while CuInS2/ZnS QDs capped with MEA exhibited the highest cytotoxicity among the three types of CuInS2/ZnS QDs modified with ligands with different terminal group. These results provided a sight on how to select surface ligands to modify CuInS2/ZnS QDs when CuInS2/ZnS QDs will apply in biological fields.
研究领域:
材料科学
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生物医学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 20
·5277-5285
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IF 3.286
Chen Li,Lin Jianwei,Yi Jinquan,Weng Qinghua,Zhou Ying,Han Zhizhong,Li Chunyan,Chen Jinghua,Zhang Qian
Rapid, highly sensitive detection of tau protein and other neurodegenerative biomarkers remains a significant hurdle for diagnostic tests for Alzheimer's disease. In this work, we developed a novel tyrosinase (TYR)-induced tau aptamer–tau–tau antibody (anti-tau) sandwich fluorescence immunoassay to detect tau protein that used dopamine (DA)-functionalized CuInS 2 /ZnS quantum dots as the fluorophore. CuInS 2 /ZnS core/shell quantum dots with high luminescence, low toxicity, and excellent biocompatibility were successfully fabricated and decorated with DA through amide conjugation. Meanwhile, TYR was conjugated with anti-tau by a click reaction. When DA-functionalized CuInS 2 /ZnS quantum dots were added to the sandwich system, TYR catalyzed the transformation of DA to dopamine quinone, which acted as an effective electron acceptor and triggered fluorescence quenching. The fluorescence intensity of the immunoassay based on DA-functionalized CuInS 2 /ZnS quantum dots shows good performance in terms of linearity with the logarithm of tau protein concentration, with a linear concentration range from 10 pM to 200 nM. This work is the first to use a TYR-induced fluorescence immunoassay for the rapid detection of tau protein, paving a new way for the detection of disease biomarkers.
Graphical abstract
研究领域:
生物化学
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纳米技术
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神经科学
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诊断学
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产品关联
DOI
PubMed
高影响因子
Oncology Letters
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2017
·Vol. 15
·期 2
·2471-2476
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IF 1.39
Guang Sun,Wanying Xing,Ren Xing,Liu Cong,Sun Tong,Siyao Yu
The aim of the present study was to develop a water-soluble biomarker for the detection of breast cancer using quantum dots (QDs) conjugated to Ki‑67, a nuclear protein associated with the cell cycle. Ki‑67 is also a marker of cell proliferation, with expression levels categorizing good and poor prognosis in invasive breast cancer. Ki‑67 is a clinically used biomarker for breast cancer diagnosis, treatment and prognosis. Owing to the optical and chemical advantages of QDs, QD‑based nanotechnology may aid the construction of a biomedical imaging platform for the study of cancer cell behavior. In the present study, a biomarker was prepared by employing the water‑soluble CuInS2/ZnS QDs conjugated to an anti‑Ki‑67 monoclonal antibody to detect Ki‑67 expression in breast cancer. The QDs, which were hydrophobic and coated with octadecylamine, were encapsulated with an amphiphilic biocompatible centipede‑like polymer, and then conjugated to anti‑Ki‑67 monoclonal antibodies (QD‑Ki‑67 probes). The QD‑Ki‑67 probes retained the original optical properties of the unadorned QDs and did not exhibit distinct toxic side effects in in vitro cytotoxicity experiments. Therefore, this CuInS2/ZnS QD‑labeled bioprobe, with a high quantum yield and low cytotoxicity, is a promising candidate for bioimaging and may be used as a cell label.
研究领域:
肿瘤学
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纳米技术
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生物医学工程
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 414
·期 5
·1829-1839
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IF 4.478
Yi Jinquan,Chen Xiaoping,Lin Jianwei,Song Kai,Han Zhizhong,Chen Jinghua
In this work, we designed new dual-mode “turn-on” electrochemical (EC) and photoelectrochemical (PEC) sensors for the detection of dopamine (DA) based on 0D/2D/2D CuInS 2 /ZnS quantum dot (QD)–black phosphorous nanosheet (BPNS)–TiO 2 nanosheet (TiO 2 NS) nanocomposites. QDs can not only improve the photocurrent of the developed PEC sensors, but also provide the electrochemical signal in the EC detection. BPNSs as p-type semiconductor with high conductive properties work as electron acceptors and are utilized to improve the sensitivity of the DA PEC and EC sensors. Under irradiation of visible light or the applied voltage, DA is both excited and releases electrons, realizing “turn-on” detection. The PEC sensors have a linear range of 0.1–100 μM with a lower detection limit of 0.028 μM. For the EC detection, BPNSs can accelerate electron transfer which attribute to its excellent conductivity. In the range of 1–200 μM, the working curve of DA detection by the EC sensors was established and the detection limit is 0.88 μM. Comparing the two methods, the PEC sensors have a lower detection limit, and the EC sensors have a wider monitoring range. The dual-mode sensors of EC and PEC pave an effective way for the detection in biological and medical fields. Graphical abstract
研究领域:
分析化学
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材料科学
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生物医学工程
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）