BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xue Hu,Xidong Liu,Xiaodan Zhang,Hongxiang Chai,Yuming Huang
The fluorescent CuNCs/ZIF-8 nanocomposites were facilely prepared by mixing the PEI protected CuNCs with the precursors of ZIF-8, and characterized by UV–vis absorption, fluorescence, FT-IR, TEM , XRD and XPS . The quantum yield of the CuNCs/ZIF-8 is 15 times that of the PEI-CuNCs. Furthermore, the CuNCs/ZIF-8 possesses better stability and higher fluorescence response due to protective and confinement effects of MOFs . It was found that H 2 O 2 could cause much more quenching of fluorescent CuNCs/ZIF-8 than that of PEI-CuNCs, which might result from enriching H 2 O 2 by ZIF-8. The CuNCs/ZIF-8 can be designed as a fluorescence probe to selectively and sensitively detect H 2 O 2 with a linear range from 0.01 to 1.5 µM and a LOD of 0.01 µM, while those with PEI-CuNCs are 0.5–30 µM and 0.50 µM, respectively. Through formation of CuNCs/ZIF-8 hybrid, the sensitivity for the detection of H 2 O 2 increases by nearly 50-fold, which makes CuNCs/ZIF-8 a desirable probe to detect H 2 O 2 content in human serum samples. Also, we successfully demonstrated the potential application of the CuNCs/ZIF-8 for screening and evaluating activities of oxidase using glucose oxidase as a model. The glucose oxidase activity can be detected in a range of 0.1–10 mU/L with a LOD of 0.1 mU/L.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
He Mei,Jingcheng Huang,Guixin Wang,Kun Chen,Huili Wang,Xuedong Wang,Wenwei Wang
Herein, we engineered a novel pH-responsive ratiometric assay by virtue of the composite system of Ce 3+ -enhanced glutathione-encapsulated copper nanoclusters (GSH-CuNCs-Ce 3+ ) and N-doped carbon quantum dots (N-CQDs). The GSH-CuNCs-Ce 3+ and N-CQDs were fabricated using one-pot chemical reduction and hydrothermal approaches, respectively, and their morphologies as well as physical and chemical properties were testified in detail by a series of characterization techniques. At 350 nm excitation wavelength, the GSH-CuNCs-Ce 3+ /N-CQDs system (GCC-NC) featured the 440/650 nm dual-emitting property. The ratios of fluorescence intensities (F 650 /F 440 ) demonstrated a strong pH-dependence from 3.0 to 5.0 with a coefficient of determination of 0.9982. Consequently, the GCC-NC system was feasible for constructing a pH-responsive dual-emitting fluoroprobe. Based on the catalytic effect of acetylcholinesterase on acetylcholine to yield acetic acid and further trigger varying solution pH, the as-constructed GCC-NC fluoroprobe was satisfactorily applied for detecting malathion . Under optimized conditions, the newly developed GCC-NC fluoroprobe supplied a wider linear range (0.25–200 μM), lower detection limit (0.075 μM), satisfactory recoveries (96.3–106.2%) and higher precision for malathion in several kinds of fruit juice samples. The fortified experiments by malathion’s structural analogues and metal ions provided compelling evidence that this fluoroprobe had strong anti-interference capacity and high specificity for malathion. These findings render us to believe that the as-constructed pH-responsive dual-emitting fluoroprobe holds great promise in trace malathion assay in food matrices.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Nuanfei Zhu,Kuijing Yuan,Dinghui Xiong,Fengxiang Ai,Kun Zeng,Biying Zhao,Zhen Zhang,Hongjun Zhao
Dietary intake is an important exposure pathway of environmental bisphenols including bisphenol S (BPS, a typical emerging organic pollutant). However, available data on the occurrence and risk assessment of BPS in food samples are limited due to the lack of high-throughput analytical methods. Herein, based on H 2 O 2 inducing fluorescence turn-off signals, a sensitive and high-throughput fluorescence enzyme-linked immunosorbent assay (FELISA) was developed for BPS investigation in beverages. In this system, the fluorescence of copper nanoclusters in ZIF-8 (CuNCs-CS@ZIF-8) could be quenched with the presence of H 2 O 2 owing to the strong oxidation, being served as signal amplification in FELISA. Meanwhile, the aggregation-induced emission (AIE) fluorescence enhancement of CuNCs-CS through the conformational locking of ZIF-8 provided an effective strategy to overcome the intrinsic limitations of FELISA including high background, serious fluorescence quenching in the detection of real samples. Thus, the integration of AIE building blocks into porous ZIF-8 showed obvious advantages in enhancing the analytical performance of FELISA. Under optimized conditions, satisfactory results with good accuracy were achieved in this proposed FELISA, which was applied for the systematic investigation of BPS in foods and further health risk assessments. The results after 100 beverages analysis displayed an average detection frequency of 55%, and the detected concentration of BPS ranged from below the limit of quantification (LOQ) to 12.08 ng/mL with a mean value of 1.717 ng/mL. Besides, the estimated daily intake (EDI) of BPS was calculated to be 0.05449–4.483 ng/kg-bw/day, in which milk–tea drinks contributed to the majority of BPS daily intake (mean EDI = 4.483 ng/kg-bw/day). And the hazard quotient (HQ) according to EDI values indicated that BPS exposure in beverages might not make a considerable impact on human health.
研究领域:
环境科学
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食品科学
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免疫学
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产品关联
DOI
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 41
·33991-34002
·
IF 4.6
Jiaxu Tian,Chenfeng Wang,Yueyue Wang,Xiaoyi Zhang,Jinxuan Pang,Chang Li,Shiying Tang
Mercury ions (Hg2+) are highly toxic and bioaccumulative, posing a serious threat to the environment and human health. Traditional analytical techniques are limited by cost, portability and real-time monitoring capabilities, and there is an urgent need for sensitive and portable detection methods. In this study, copper nanoclusters (CuNCs) were encapsulated in red-emitting europium metal–organic frameworks (Eu-MOFs), and a ratiometric fluorescence probe based on CuNCs@EuMOFs was constructed and used for the detection of Hg2+ in water extracts of traditional Chinese medicine samples and river samples. In the presence of Hg2+, the fluorescence of this probe is enhanced at 450 nm and weakened at 618 nm. Visual quantification can be performed using a smart phone based on the color change. The linear range is 0–360 μM (R2 = 0.9986), and the detection limit is 0.94 nM. The sample recovery rate was 94.43–106.43% (RSD < 4.37%). This study provides a new strategy for the portable visual detection of Hg2+ in complex matrices.
研究领域:
环境监测
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分析化学
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纳米材料
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荧光传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Guirong Liu,Wenli He,Chenghui Liu
As an important base excision repair (BER) enzyme, uracil-DNA glycosylase (UDG) can repair the uracil-induced DNA lesion and maintain the genomic integrity. Herein, we have proposed a sensitive UDG assay based on the terminal deoxynucleotidyl transferase (TdT)-assisted formation of fluorescent copper nanoclusters (CuNCs). In this study, a uracil-containing stem-loop DNA substrate is rationally designed with its 3′-end blocked with 2′, 3′-dideoxycytosine (ddC). UDG can remove the uracil in the DNA substrate to generate an apurinic/apyrimidinic (AP) site, which can then be specifically cleaved by endonuclease IV (Endo IV) to expose a 3′-OH terminus. TdT will initiate the template-free DNA extension along the exposed 3′-OH terminus to produce a quite long poly(T) tail, which will perfectly template the production of fluorescent CuNCs. By recording the fluorescence of the CuNCs, the UDG activity can be faithfully detected. In contrast, if UDG is absent, the 3′-ddC terminus of the DNA substrate cannot be recognized by TdT and thus no TdT-based extension and formation of CuNCs will occur. The use of ddC as a 3′-end blocker can greatly decrease the nonspecific DNA extension and improve the signal-to-noise ratio. Furthermore, TdT is a template-free and sequence-independent DNA polymerase, which can effectively catalyze the tailing process up to a maximum of thousands of thymines , and each tail can form many fluorescent CuNCs. Therefore, an ultrahigh sensitivity is achieved and as low as 0.00005 U/mL of UDG can be clearly detected. Moreover, with an additional AP site-contained poly(A) oligonucleotide during TdT-mediated extension, a branched amplification mechanism is also preliminarily devised, which can further push the detection limit of UDG to an extremely low level of 0.000002 U/mL.
研究领域:
生物化学
·
分子生物学
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分析化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Shanshan Wang,Yingyi Wang,Yi Peng,Xiaoming Yang
Integrating kinds of bactericides as one entirety shows prospect for improving antibacterial efficiency, thus a new type of nanoclusters based on bactericide-directed herein emerged as a combination for achieving a higher antibacterial effect. Specifically, an easily operated approach for preparing bacitracin-directed silver, gold, and copper nanoclusters (AgNCs, AuNCs, and CuNCs, respectively) was proposed. Meanwhile, AgNCs@Bacitracin, AuNCs@Bacitracin, and CuNCs@Bacitracin emitted cyan, yellow, and red colors of fluorescence, respectively, and different sizes and nitrogen contents of these nanoclusters played a critical role for their fluorescence alterations. Significantly, AgNCs@Bacitracin exhibited robust bacteria-killing efficiency than other nanoclusters, owing to its marked damage on the bacterial membrane. Additionally, propidium iodide (PI) staining indicated the bacterial membrane damage of 72.3% to the bacteria population followed by the treatment with AgNCs@Bacitracin, and the introduction of AgNCs@Bacitracin lead to the higher reactive oxygen species (ROS) production, facilitating their attractive antibacterial activity. Moreover, this strategy of developing new nanoclusters broadened the avenues for designing the improved antibacterial materials.
研究领域:
纳米技术
·
材料科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2025
·Vol. 40
·期 12
·e70396
·
IF 3
Jinhua Fu,Gengneng Lai,Fang Ke,Zhibin Zhao,Xiuzhi Xu
Acid phosphatase (ACP) serves as a critical biomarker for disease diagnosis, yet conventional detection methods face limitations in cost and complexity. Copper nanoclusters (CuNCs), leveraging Earth-abundance, low expense, and noble metal-like catalytic properties, offer an ideal nanozyme platform. This study harnesses the intrinsic peroxidase mimicking activity of CuNCs to construct a visual colorimetric sensor for ACP detection. CuNCs oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue chromogen (λmax = 654 nm). ACP introduction triggers reduction of this chromogen, diminishing both color intensity and absorbance. The optimized sensor achieves a linear detection range of 0–6 U/L with a detection limit of 0.027 U/L, demonstrating high sensitivity and specificity. This CuNCs-based platform provides a rapid, low-cost, and operationally simple approach for ACP quantification, highlighting significant potential for clinical diagnostics.
研究领域:
生物化学
·
纳米技术
·
临床诊断
·
酶检测
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2021
·
IF 3.882
Jin Yuan,Wenna Wu,Luxuan Guo,Jingcheng Hao,Shuli Dong
Multistimuli-responsive fluorescent gelsbased small molecular gelator by supramolecular assembly, possessing excellent dynamic and reversible characteristic, have caused much concern. In this article, aggregation-induced emission-active fluorescence gels (AIE-gels) with chirality were developed by combining Cu nanoclusters (CuNCs) and natural amino acids, l-tryptophan (l-Trp) or d-Tryptophan (d-Trp). In DMSO/H2O mixed solvents, CuNCs can self-assemble to form intertwined fibersbased nanoparticles with numerous pores by introducing Zn2+. Fibers as second networks of heteronetwork structures are characterized with the participation of l-Trp or d-Trp for cross-linking to reinforce mechanical strength and chiral regulation of gel networks. Aggregation-induced emission enhancement (AIEE) of CuNCs endows the gels with excellent fluorescent properties by introducing solvents and gelation process. The fluorescent gels exhibit sufficient fluorescence intensity (FI) at −20 °C to −80 °C and possess sensitive responsibility including gel–sol transition and fluorescence behavior for stimuli of mechanical force, heating, pH, H2O2, and ethylene diamine tetraacetic acid (EDTA).
研究领域:
材料科学
·
化学
·
生物化学
✓ 已验证
DOI
高影响因子
LUMINESCENCE
·
2026
·Vol. 41
·期 3
·e70462
·
IF 2.8
Rui Tian,Yuhui Yang,Duo Li,Jiamin Chai,Rui Zhang,Hua Yang,Haoyu Li,Xuehua Sun
Polyvinylpyrrolidone (PVP) and cysteine (Cys) double-ligand–modified copper nanoclusters (PVP-Cys CuNCs) were synthesized by a one-pot method. The addition of Cys can protect copper nanoclusters (CuNCs) more effectively and enhance its fluorescence intensity, compared with the single-ligand-modified PVP CuNCs; the double-ligand-modified PVP-Cys CuNCs showed higher fluorescence intensity and sensing ability. Based on the internal filtration and dynamic quenching effect of berberine (BBR) on PVP-Cys CuNCs, a novel, simple, and sensitive method for BBR detection has been established. This method exhibits a good linear response to BBR within the linear range of 1.09 × 10 −7 –4.26 × 10 −6 mol · L −1 , and with a limit of detection (LOD) of 3.67 × 10 −8 mol · L −1 . It has been successfully applied to the determination of BBR in practical pharmaceutical samples.
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Shan Pu,Chengyan Xia,Lan Wu,Kailai Xu
In this study, polyvinylpyrrolidone (PVP) supported and cysteine (Cys) modified copper nanoclusters (Cys-PVP-CuNCs) with high fluorescence intensity have been synthesized and used to establish a simple and sensitive assay for the determination of trace Sn 4+ . The presence of Sn 4+ can lead to fluorescence quenching based on the aggregation of PVP-CuNCs caused by the coordination of Sn 4+ and oxygen atoms. For Cys-PVP-CuNCs, the ligand Cys can transfer electrons to the metal center of the cluster, resulting in strong fluorescence enhancement of CuNCs and intensification of fluorescence quenching after interaction with Sn 4+ , which is conducive to more sensitive visual detection. The proposed assay allows for the detection of Sn 4+ in a linear range from 5 μg L −1 to 100 μg L −1 , with a detection limit as low as 4.5 μg L −1 at a signal-to-noise ratio of 3. Moreover, under UV light, the obvious fluorescent color change can be observed by naked eyes at Sn 4+ concentration ranging from as low as 5 μg L −1 to 200 μg L −1 . Benefiting from the high sensitivity of this assay, satisfactory test results have been obtained when using only 10 mg of canned food samples with rapid digestion. The proposed Cys-PVP-CuNCs fluorescent probe shows excellent stability, simple operation, good selectivity, and high sensitivity, and thus it is expected to be used in the real-time field detection of Sn 4+ .
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Liyang Fu,Huimin Liu,Liang Yan,Yanyan Fu,Yu Zhu,Lan Jin,Ruizheng Liang
Fluorescent copper nanoclusters (CuNCs) have been drawing great research interest because of their fascinating physicochemical properties. However, the low quantum yield (QY) and poor stability of CuNCs have limited their applications. In this work, CuNCs were anchored onto the layered double hydroxides (LDHs) by the layer-by-layer (LBL) assembly method, and the obtained CuNCs/LDHs ultrathin films displayed an ordered periodic long-range structure and uniform morphology. The CuNCs/LDHs films indicate the strong orange-red emission with higher QY (16.66%) and prolonged fluorescence lifetime (9.05 μs), which is obviously better than that of individual CuNCs. The experimental studies and theoretical calculations both reveal that the location and confinement effects derived from LDHs nanosheets enhance the QY of CuNCs. In addition, the CuNCs/LDHs films are temperature-responsive in photoluminescence (PL) and electrogenerated chemiluminescence (ECL). Consequently, it provides a new and facile approach to fabricate CuNCs-based films accompanied by excellent luminescent properties, which indicate the great potential for temperature sensing applications.
研究领域:
材料科学
·
光学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Songrui Li,Fubin Yang,Junying Li,Yongcun Zou,Qiong Jia
Background As a common organophosphorus pesticide, trichlorfon (TC) is being extensively used in agriculture due to its high efficiency in plant diseases and insect pest control. However, TC should be monitored in a timely manner due to its severe threat to public health. Currently reported fluorescence methods for TC detection largely depend on enzyme inhibition effect, which suffers from their intrinsic defects such as poor selectivity, complexity, or high cost. Hence, it is of essential value to develop a facile and low-cost fluorescence approach to achieve specific enzyme-free detection of TC. Results In this work, L-cysteine protected copper nanoclusters (CuNCs) with good stability were prepared as the fluorescence sensing probe. Under alkaline conditions, TC tends to be hydrolyzed to produce dichloroacetaldehyde, which was directly employed as an effective reducing agent to in-situ synthesize silver nanoparticles (AgNPs). The fluorescence of L-cys-CuNCs was quenched by AgNPs through the inner filter effect (IFE), realizing specific enzyme-free determination of TC. Besides, portable and visual detection of TC was achieved depending on a smartphone sensing platform. This method was further employed to monitor TC in cherry tomatoes, potatoes, and cultivated soil samples with satisfying results, illustrating its high potential in monitoring TC in real samples. Overall, this method possesses the attractive superiorities of simplicity, cost efficiency, and specificity for the detection of TC. Significance The strategy has the following advantages. (i) Specific enzyme-free detection of TC was achieved based on CuNCs/AgNPs fluorescent platform. (ii) AgNPs, as CuNCs fluorescence quencher, was directly in-situ synthesized using the hydrolysis products of TC, which avoids adding extra reducing agent and provides a possibility for the waste utilization of TC. (iii) A portable and low-cost smartphone sensing platform was constructed for visually specific determination of TC.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）