Yudie Lv,Yang Yang,Jiaxin Niu,Xu Sun,Han Zhang,Xiangdong Kong
Oxidative stress is closely linked to androgenetic alopecia (AGA). However, research on antioxidants specifically targeting hair follicle tissue has been limited. The utilization of nanomaterials that possess superior reactive oxygen species (ROS)-scavenging capabilities and biocompatibility is a promising approach for the treatment of AGA. The microneedle-mediated transdermal delivery system can overcome the limitations of the stratum corneum and effectively deliver functional nanomaterials to the vicinity of hair follicles. In this study, we report a degradable microneedle patch loaded with ROS-scavenging CuxO nanozyme (CuxO-MNs) for targeted treatment of AGA. Tiny CuxO nanozymes were synthesized using a robust and eco-friendly method, demonstrating effective ROS-scavenging ability at both in vitro and cellular levels. With the penetration and dissolution of sodium hyaluronate microneedles, CuxO can be rapidly released and effectively reach the area around the hair follicle, enabling targeted removal of ROS. In the AGA model, CuxO-MNs effectively alleviate oxidative stress, restore the hair follicle microenvironment, and stimulate the transition of hair follicles from the telogen to the anagen. These combined effects significantly enhance hair regeneration and exhibit therapeutic efficacy comparable to the clinical drug minoxidil, suggesting the safety and translational potential of this strategy for AGA treatment.
Ke Wang,Jian Liu,Xin Wang,Xiaoyang Liu,Jinshuang Hu,Enshou Li,Yusai Zhao,Rusong Zhao,Shenghong Yang
Copper oxide nanoparticles (CuO NPs) as nanozymes have received extensive attention due to their numerous advantages such as water solubility and biomimetic effects. In this work, we combined the inherent oxidase and ascorbate oxidase mimetic activity of CuO NPs to construct a ratiometric fluorescence system for the detection of ascorbic acid (Vitamin C, AA) in serum and fruits. By feat of the dual mimic nanozymatic activity of CuO NPs, the sole substrate o-phenylenediamine (OPD) can be catalyzed to attain yellow fluorescent 2,3-diaminophenazine (oxOPD), while oxidized AA will react with the pristine OPD to form blue fluorescent product. This method has a sensitive response to AA with a good linear relationship in the concentration range of 1–150 μM and low detection limit of 0.16 μM. Practical sample detection of AA in human serum and fruits can be realized reasonably with the recovery rates between 84.83% and 87.28% and 88.53%-113.1%, respectively. More conveniently, the method can be successfully used for scanning measurement analysis through the associated application (ColorAssist) on smartphones and portable UV lamps.
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