Particulate photocatalysts developed for the solar energy-driven reduction of the greenhouse gas CO 2 have a small product range and low specificity. Hybrid photosynthesis expands the number of products with photocatalysts harvesting sunlight and transferring charges to microbes harboring versatile metabolisms for bioproduction. Besides CO 2 , abiotic photocatalysts have been employed to increase microbial production yields of reduced compounds from organic carbon substrates. Most single-reactor hybrid photosynthesis systems comprise CdS assembled in situ by microbial activity. This approach limits optimization of the morphology, crystal structure, and crystallinity of CdS for higher performance, which is usually done via synthesis methods incompatible with life. Here, shape and activity optimized CdS nanorods were hydrothermally produced and subsequently applied to Cupriavidus necator for the heterotrophic and autotrophic production of the bioplastic polyhydroxybutyrate (PHB). C. necator with CdS NR under light produced 1.5 times more PHB when compared to the same bacterium with suboptimal commercially-available CdS. Illuminated C. necator with CdS NR synthesized 1.41 g PHB from fructose over 120 h and 28 mg PHB from CO 2 over 48 h. Interestingly, the beneficial effect of CdS NR was specific to C. necator as the metabolism of other microbes often employed for bioproduction including yeast and bacteria was negatively impacted. These results demonstrate that hybrid photosynthesis is more productive when the photocatalyst characteristics are optimized via a separated synthesis process prior to being coupled with microbes. Furthermore, bioproduction improvement by CdS-based photocatalyst requires specific microbial species highlighting the importance of screening efforts for the development of performant hybrid photosynthesis.
The photocatalyst graphitic carbon nitride (g-C 3 N 4 ) is known to photostimulate the production of the bioplastic polyhydroxybutyrate (PHB) by Cupriavidus necator . In previous studies, the combination of C. necator and g-C 3 N 4 increased PHB yield from either an organic or inorganic carbon substrate under a light intensity of 4200 lx. Here, different parameters including light intensity, pH, temperature, nitrogen and carbon concentrations, aeration, and inoculum size were explored to maximize PHB production by hybrid photosynthesis from fructose and visible light. A g-C 3 N 4 / C. necator culture grown with a lower light intensity of 2100 lx, an inoculum size of 128.30 × 10 6 CFU ml −1 , and constant aeration produced 7.16 g l −1 d −1 PHB with a product yield from fructose of 60.94%. Furthermore, the ratio of incident photons harvested by g-C 3 N 4 converted into NADPH+H + by C. necator for PHB production was improved to 19.74% after the process optimization. In comparison, the PHB production rate of a non-optimized g-C 3 N 4 / C. necator system exposed to 4200 lx was only 2.94 g l −1 d −1 with a product yield from fructose of 33.29%. These results demonstrate that hybrid photosynthesis productivity can be significantly augmented by decreasing light intensity and adjusting other parameters, which is promising for future bioproduction applications.
Li Xingjie,Xiao Qiusheng,Shao Qin,Li Xiaopeng,Kong Jiejie,Liu Liyan,Zhao Zhigang,Li Rungen
Biosorbents have been extensively studied for heavy metal adsorption due to their advantages of low cost and high efficiency. In the study, the living and non-living biomass of Cupriavidus necator GX_5 previously isolated were evaluated for their adsorption capacity and/or removal efficiency for Cd (II) through batch experiments, SEM and FT-IR investigations. The maximum removal efficiency rates for the live and dead biomass were 60.51% and 78.53%, respectively, at an optimum pH of 6, a dosage of 1 g/L and an initial Cd (II) concentration of 5 mg/L. The pseudo-second-order kinetic model was more suitable for fitting the experimental data, indicating that the rate-limiting step might be chemisorption. The Freundlich isotherm model fit better than the Langmuir isotherm model, implying that the adsorption process of both biosorbents was heterogeneous. FT-IR observation reflected that various functional groups were involved in Cd (II) adsorption: –OH, –NH, C=O, C–O and C–C groups for the living biomass and –OH, –NH, C–H, C = O, C–N and N–H groups for the dead biomass. Our results imply that non-living biosorbents have a higher capacity and stronger strength for absorbing Cd (II) than living biomass. Therefore, we suggest that dead GX_5 is a promising adsorbent and can be used in Cd (II)-contaminated environments.
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