SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Jing Xu,Huanhuan Li,Selva Sharma Arumugam,Yawen Rong,Pingyue Wang,Quansheng Chen
Adenosine triphosphate (ATP) is an energy molecule of significant importance, and, the monitoring of ATP in living cells is considerable for the clinical diagnosis of many related diseases, including cancer. Upconversion nanoparticles (UCNPs) have recently been attracting widespread interest in biomedical applications due to their chemical and thermal stability, high sensitivity, good biocompatibility, and excellent tissue penetration. Herein, a Cy3-aptamer-cDNA- UCNPs nanosensor was synthesized, based on the luminescence resonance energy transfer (LRET) between UCNPs and Cy3 for monitoring ATP in living cells. It showed a selective sensing ability for ATP levels by changes of fluorescence intensity of UNCPs at 536 nm. The investigated biosensor showed a precise, efficient detection with sufficient selectivity which was achieved through the optimization of conditions. In the range of 1–1000 μM, the ATP-induced changes of the fluorescence intensity were linearly proportional to the ATP concentrations. Furthermore, the cytotoxicity assay revealed that the UCNPs sensor exhibited favorable biocompatibility, implicating the use of UCNPs in vivo imaging. This study highlights the potential of using a combination of UCNPs and ATP-binding aptamer to design an ATP-activatable probe for fluorescence-mediated imaging in living cells. These results implied that the nanosensor can be applicable for the monitoring of intracellular ATP by fluorescence imaging and the quantitative analysis of biological liquids.
研究领域:
生物医学工程
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分析化学
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分子生物学
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细胞生物学
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生物技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
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IF 5.399
Tarun Belwal,Hao Huang,Li Li,Zhenhua Duan,Xuebing Zhang,Halah Aalim,Zisheng Luo
Ultrasonic effect on extraction of cyanidin-3-galactoside (Cy3-gal) from pear fruit peel was investigated and compared with conventional extraction (CE) method. Different process factors were tested to determine the optimum conditions for ultrasonic-assisted extraction (UAE). Results revealed that under optimized UAE conditions (ultrasonic power = 162 W, temperature = 71 °C, trifluoroacetic acid = 3%, ethanol = 57%, ultrasonication time = 11 min, and sample to solvent ratio = 1:30 g/ml), Cy3-gal yield was significantly higher (0.34 3± 0.005 mg/g) than from CE (0.266 ± 0.004 mg/g), whereas the extract viscosity during UAE showed a negative impact. UPLC-Triple-TOF/MS analysis detected a total number of 13 anthocyanin compounds, out of which 8 were identified and that mainly consisted of cyanidin, delphinidin and petunidin compounds. Higher yield of Cy3-gal under UAE compared to CE was also justified by higher deformations in the cell structure. The possible mechanism of ultrasonication effect during the extraction process is also proposed in the present study. During scale-up UAE process, the extraction yield of Cy3-gal was recorded higher under batch scale-up compared to continuous operation. The present study is an attempt to optimize UAE of valuable anthocyanins from Pyrus communis ‘Starkrimson’ fruit peel and further scaled-up for higher volume extraction which can be utilized for industrial applications.
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2021
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IF 6.057
Tongtong Li,Yan Zhang,Xiaohong Sun,Yanjin Zhang,Yongan Wang,Zhiyong Nie
A fluorescent probe was developed for ratiometric detection of thallium ions in mineral water samples by modifying a G-rich aptamer (PS2.M − 7) with a fluorescence donor (Cyanine-3, Cy 3 ) and a quencher (Cyanine-5, Cy 5 ). The probe had a random coil structure that changed into a G-quadruplex structure upon binding with Tl + . This change in structure decreased the distance between the donor and acceptor moieties, which resulted in fluorescence resonance energy transfer between Cy 3 and Cy 5 . Under optimized conditions, the limit of detection and linear concentration range for Tl + were 30.1 μM (3σ) and 10 μM–10 mM ( R 2 = 0.9981), respectively. This simple and cost-effective fluorescence sensor provided satisfactory results for detection of thallium ions in spiked mineral water samples.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2018
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IF 4.244
Hongbin Pu,Xiaohui Xie,Da-Wen Sun,Qingyi Wei,Yingfen Jiang
A detection method for 17β-estradiol (E2) using surface-enhanced Raman scattering (SERS)-based aptamer sensor was presented. Raman reporter molecule Cy3 labeled E2-aptamer and DNA functionalized gold-silver core-shell nanoparticles ( [email protected] CS NPs) offered SERS with high sensitivity and selectivity . Based on the fabricated double strand DNA-immobilized gold-silver core-shell nanoparticles ( [email protected] NPs), SERS signal intensity of Raman reporter changed with the number of Cy3-labeled aptamer attached to the core-shell nanoparticles due to the strong binding affinity between the aptamers and E2 with different concentrations. A wide linear range from 1.0 × 10 –13 to 1.0 × 10 −9 was obtained for the detection of E2, with a low detection limit of 2.75 fM. This proposed method showed highly sensitive and selective for detecting E2, and could be used to determine E2 in actual samples.
研究领域:
分析化学
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纳米技术
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
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IF 4.916
Yingfen Jiang,Da-Wen Sun,Hongbin Pu,Qingyi Wei
An ultrasensitive method for the kanamycin (KANA) detection in milk sample using surface-enhanced Raman spectroscopy-based aptasensor was employed in the current study. Double strand DNA binding bimetallic [email protected] nanoparticles were developed as a sensing platform. Probe DNAs were first embedded on the surface of gold nanoparticles by the end-modified thiol, and after silver shell encapsulating, KANA aptamer DNAs with the Raman reporter Cy3 were then hybridized with probe DNAs by complementary base pairing. Results showed that with increase in the KANA concentration, the Raman intensity of Cy3 decreased. Besides achieving selectivity , an ultralow detection limit of 0.90 pg/mL, a broad linear relationship ranging from 10 μg/mL to 100 ng/mL in aqueous reagent and satisfactory recoveries of 90.4–112% in liquid whole milk were obtained. The result of actual sample proved that this aptasensor was promising in trace determination of KANA residue.
研究领域:
分析化学
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食品安全
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2021
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IF 5.097
Li Ping Cao,Yao Wang,Yan Bai,Yong Jian Jiang,Chun Mei Li,Hua Zuo,Yuan Fang Li,Shu Jun Zhen,Cheng Zhi Huang
Inspired by the specific optical properties of DNA photonic nanowires (PNs) on which fluorescent dyes are to be precisely positioned and photonic energy transfer over nanometers can be achieved, herein, we develop a DNA PN-based homogeneous entropy-driven biomolecular assay (HEBA). Using homogeneous Förster resonance energy transfer (FRET) of Cy3, we constructed a DNA PN capable of transferring energy from the green donor dye (FAM) to the red acceptor fluorophore (Cy5) across a total distance of 10.2 nm with an end-to-end transfer efficiency of 13.2%. By taking DNA PNs as the substrate of entropy-driven circuit, thrombin was detected as a proof-of-principle target. The trigger of HEBA by thrombin, during which separation of Cy3, FAM, and Cy5 occurred and the FRET of the PN got interrupted, resulted in a high value of signal-to-background ratio (S/B, signal and background were FFAM/FCy5 in the presence and absence of thrombin, respectively), giving a detection dynamic range of 5–25 nM with a detection limit of 2.7 nM. This strategy offers an alternative platform for biomarker clinical diagnosis, greatly expanding the application fields of DNA PNs to analytical chemistry.
研究领域:
生物技术
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分析化学
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纳米技术
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2015
·Vol. 25
·期 5
·1267-1277
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IF 1.927
Huang Weidong,Wang Yue,Wang Zhimin
Single-molecule detection (SMD) and single-molecule fluorescence resonance energy transfer (smFRET) were conducted using Cy3- and Cy5-labeled single-strand DNAs (ssDNAs) either immobilized on substrates or encapsulated in microdroplets. High-quality fluorescent images were obtained using a total internal reflection fluorescence microscope (TIRFM). In the substrate system, deposition of a low concentration of fluorescence molecules on substrates through electrostatic adsorption showed that most of the fluorescence spots were single molecules, and the mean value of signal to noise ratio (S/N) reached 6.9 ± 0.34. smFRET analysis was conducted through immobilization of donor- and acceptor-labeled oligonucleotides on substrates. In the droplet system, fluorophor-labeled oligonucleotides were injected into T-type microfluidics. Single and double fluorophor-labeled DNA molecules encapsulated in droplets were detected, the FRET efficiency and inter-dye distance of a single donor-acceptor pair were measured accurately. smFRET was conducted detailedly in the tortuous channel for the first time.
研究领域:
生物化学
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分子生物学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
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