Chenping Li,Juncheng Zhu,Shihao Qiao,Yuxin Yang,Hongjie Dai,Hai Chen,Liang Ma,Yuhao Zhang,Hongxia Wang
Gelatin based nanoparticles was prepared by sodium trimetaphosphate (STMP) for stabilizing and fabricating high internal phase emulsions (HIPEs), and cyanophycin and capsanthin were single/dual-encapsulated in emulsion systems. The characterization of FTIR, XRD, TGA, zeta potential, particle sizes, dilatation rheology and AFM indicated that the phosphate groups of STMP could react with side chain groups of gelatin and chitosan to form nanoparticles, and enhance the thermal resistance. The nanoparticles could form strong membrane on the oil-water interface, which prevents stratification and precipitation of HIPEs, and promotes high centrifugation stability and freezing-thawing stability. The functional HIPEs with single encapsulation of medium-content cyanophycin or capsanthin have excellent colors, shear thinning performance, structure reversibility, mechanical strength, elasticity and strong network systems with small emulsion droplets; however, the mixed purple HIPEs illustrated the relative weak network structure and the low final strain recovery rates with the enlarged emulsion droplets. On another hand, the simultaneous load of cyanophycin and capsanthin in emulsion systems could produce bright purple HIPEs based on Subtractive Color Principle, and HIPEs possess the outstanding rheological characteristics and the structured systems with small emulsion droplets. The outstanding extrudability and excellent combinational 3D printability of functional HIPEs suggest the great potential in personalized, innovative food production.
Zou Kui,Liu Hongyuan,Feng Bo,Qing Taiping,Zhang Peng
In the sewage treatment process, facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation. Cyanophycin granule polypeptide (CGP), a recently discovered high value-added biopolymer present in activated sludge, has provided new avenues for the recovery of resources. However, the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus far. This study investigated the synthesis of CGP, polyhydroxyalkanoates (PHA), and alginate-like exopolysaccharides (ALE) in various microbial aggregates under different carbon sources feeding conditions. Our results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding conditions. Furthermore, biofilm was more conducive to CGP accumulation than floc sludge. Compared with glucose and methanol, sodium acetate significantly enriched the CGP synthetase-encoding gene ( cphA abundance = ~17419), resulting in the highest CGP yield (average 107.1 mg/g MLSS) in both biofilm and floc sludge. This study is the first to reported the characteristic fluorescence of CGP (Ex/Em = ~360/450 nm) caused by the aggregated luminescence of arginine on the side chains. Overall, this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.
危险品化学品经营许可证（带存储）