GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2020
·
IF 2.428
Liping Yang,Shaoyang Zhi,Guokun Yang,Chaobin Qin,Wenli Zhao,Mingming Niu,Wenlei Zhang,Wenyu Tang,Xiao Yan,Yuru Zhang,Xiaolin Meng,Ronghua Lu,Guoxing Nie
Irisin, encoded by fibronectin type III domain-containing protein 5 (FNDC5) gene, plays a role in energy expenditure and insulin sensitivity in mice. In fish, the function of irisin related to glucose metabolism is less reported. It may increase glucose utilization in fish. The aim of the present study was to characterize the regulatory role of irisin in glucose metabolism in common carp ( Cyprinus carpio L.). In this study, FNDC5a and FNDC5b were isolated from common carp. The cDNA of FNDC5a and FNDC5b were 722 bp and 714 bp, encoding 221 and 207 amino acids, respectively. FNDC5a was abundantly expressed in the brain and gonad. FNDC5b was mainly expressed in brain. Different expression pattern of FNDC5a and FNDC5b under fasting/refeeding and OGTT experiment were identified. The recombinant common carp irisinA and irisinB were prepared by prokaryotic expression system. Glucose concentration was decreased in treatment with irisinA or irisinB in the in vitro and in vivo experiments. The mRNA expression levels of gluconeogenesis-related genes were significantly down-regulated, while the mRNA expression of glycolysis-related genes were significantly up-regulated after treatment with recombinant irisinA or irisinB in liver in vivo and in primary hepatocytes in vitro. Our research shows that irisin inhibits hepatic gluconeogenesis and promotes hepatic glycolysis. Taken together, this study for the first time revealed the two subtypes of FNDC5 and explored the function and mechanisms of irisinA and irisinB in fish glucose homeostasis.
研究领域:
分子生物学
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内分泌学
·
水产养殖
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Feixue Liu,Tianmeng Zhang,Yeyun Ma,Jingquan Dong,Yong Sun
Environmental residues of the fungicide difenoconazole (DFZ) have been shown to pose a threat to mammals. However, the risk assessment of DFZ for cultured carp remains unclear. The aim of this study was to investigate the adverse effects of DFZ on carp liver and their molecular mechanisms by simulating the environmental contamination concentrations of DFZ. Our results showed that DFZ induced structural damage in the liver, including edema, vacuolation, and congestion. In addition, DFZ residues were detected in liver tissues. Mechanistically, DFZ causes mitochondrial dysfunction by promoting Ca 2+ transfer from the endoplasmic reticulum (ER) to mitochondria via IP3R, leading to the onset of ROS burst and apoptosis, and the inhibition of Nrf2 antioxidant function by DFZ also results in uncontrolled ROS. Mitophagy was also activated intracellularly to counteract mitochondrial damage. Interestingly, treatment with 2-APB alleviated mitochondrial dysfunction, restored the mitochondrial membrane potential, and inhibited apoptosis by blocking the translocation of Ca 2+ from the ER to the mitochondria. Metabolomic analysis revealed that DFZ disrupted energy metabolism in carp liver, whereas 2-APB reversed DFZ-induced metabolic alterations. In conclusion, the present study elucidates the threat of DFZ to carp liver and highlights the mechanism of damage, thereby helping to explain the impact of agriculture on the aquatic environment.
研究领域:
环境科学
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毒理学
·
水生生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xiaojing Li,Qian Wu,Dan Chen,Yichen Bai,Yuhong Yang,Shiwen Xu
Cadmium (Cd) is a major contaminant in natural environments and exerts adverse effects on aquatic biota at low concentrations. Gill is as vital respiratory organ and may cause pollutants to enter fish during gas exchange. Baicalein (BAI), as a kind of flavonoids, possess antioxidant properties through inactivating free radicals. To confirm the potential effects and approaches of BAI addition in maintaining the gill stability, 90 common carps ( Cyprinus carpio L.) were selected and randomly divided into water environment exposure group (0.22 mg/L Cd) and/or feed added with 0.10 g/kg BAI for 30 days. The analysis of ion content in serum showed that Cd exposure disturbed ion homeostasis , and BAI could reduce serum Cd concentration. The histopathological results of gills showed that Cd exposure caused gill tissue lesions and structural damage, and BAI feeding effectively alleviated this damage. In addition, BAI could enhance antioxidant activity and activate Nrf2/HO-1 axis, thereby reducing oxidative stress and endoplasmic reticulum (ER) stress. Moreover, BAI lightened cytokine imbalance, inflammatory response, and necroptosis. Overall, the results indicated that BAI feeding could maintain gill homeostasis against Cd poisoning via the ROS/ER stress signaling. This trial revealed the properties of BAI resistance to metal Cd in aquaculture and partially elucidated its mechanism.
研究领域:
水生毒理学
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环境科学
·
药理学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·Vol. 2024
·期 1
·5205506
·
IF 3
Dan Liu,Jiali Mi,Xiao Yan,Chaobin Qin,Junli Wang,Guoxing Nie
In the present study, we conducted a 10-week culture experiment to investigate the effects of taurine on the growth performance, antioxidant properties, and muscle quality of the common carp fed an oxidized lipid diet. There were five experimental groups with three replicates each. Based on the fresh fish oil group (FO), equal amounts of oxidized fish oil (with a thiobarbituric acid-reactive substances value of 49.57 ± 2.34 mg/kg) and 0 g/kg (OFO), 4 g/kg (OT4), 8 g/kg (OT8), and 12 g/kg (OT12) taurine were added to the diet, while the same composition ratio was maintained by changing the microcrystalline cellulose content. Compared to the FO group, the feed conversion ratio, weight gain rate, muscle crude lipid, and n-3/n-6 polyunsaturated fatty acids (PUFA) ratio were significantly lower in the group OFO ( P < 0.05). In addition, compared to the FO group, OFO fish showed an increased content of malondialdehyde and protein carbonylation and decreased hardness, brightness, pH, superoxide dismutase, and catalase levels in the muscle ( P < 0.05). Notably, the growth index significantly improved in the OT4 group compared to that in the OFO group ( P < 0.05). In addition, dietary taurine increased the crude lipid content, n-3/n-6 PUFA, antioxidant capacity, hardness, springiness, brightness, pH, and collagen content in the muscle compared with OFO fish ( P < 0.05). Moreover, taurine supplementation significantly reduced myofiber diameter and increased myofiber density ( P < 0.05) and enhanced the expression levels of paired box 7 ( pax7 ), myogenic factor 5 ( myf5 ), myogenic differentiation antigen ( myod ), and myogenic regulatory factor 4 ( mrf4 ) compared with that of the OFO group ( P < 0.05). Considering growth performance and muscle quality, the optimal supplemental levels of taurine in the oxidized lipid diet were 4 g/kg and 6.84–7.70 g/kg, respectively.
研究领域:
水产养殖
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2024
·
IF 2.6
Yujing Ren,Xiubin Hou,Ying Zhang,Jingyan Hao,Juan Gao,Xinbing Song,Yurong Ma
Anhydrous guanine (AG) crystals are widely applied in organisms due to their excellent optical properties. However, the influence of biomolecules on the biomineralization process of guanine crystals in organisms remains unexplored. In this study, mixed proteins from the silver film of carp fish scales were extracted and were used as a model system to elucidate the effect of biomolecules on the guanine crystallization for the first time. The mixed proteins were enriched with amino acids such as Asp, Glu, Leu and Lys. Elongated hexagonal microplates of α-form AG (α-AG) mixed with a small amount of β-form AG (β-AG) mainly exposing (100) plane were obtained in the presence of the mixed proteins under mild condition while irregular particles were formed in the absence of proteins, indicating that the extracted mixed proteins have a strong influence on the formation of AG microplates exposing (100) plane. It is found that the proteins not only adsorb on the surface but also occluded into the AG crystals by using confocal laser scanning microscopy analysis. We also find that guanine analogues have profound synergetic effects on the morphology and polymorphs of guanine in the in-vitro synthetic system. The obtained α-AG exhibit superior optical properties such as high reflectivity, strong depolarization and pearlescent luster. This in-vitro synthesis work shows clearly the strong influence of biomolecules for the biomineralization process of guanine crystals in the organisms.
研究领域:
生物矿化
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材料科学
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生物化学
·
光学
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产品关联
DOI
高影响因子
Biology-Basel
·
2025
·Vol. 14
·期 9
·1303
·
IF 3.5
Tengyu Liu,Rui Han,Yuhan Jiang,Jiamin Sun,Haiyun Wu,Qigen Liu
Water depth is a crucial environmental factor influencing temperature, oxygen availability, light intensity, and ultimately the physiology, behavior, and distribution of aquatic animals. This study investigated the physiological responses of two common carp varieties—the Qingtian paddy field carp, a core component of the Qingtian rice-fish co-culture system, and the Xingguo red carp, a typical pond-domesticated species—to extreme shallow aquatic environments (5–20 cm depth). Since traditional blood sampling fails to capture dynamic stress levels, we used a real-time glucose biosensor combined with biochemical assays to continuously monitor stress and adaptive reactions. The results show that the Qingtian carp exhibits superior adaptation to shallow water conditions, marked by higher stress tolerance, faster recovery, and efficient lipid-based energy utilization. These findings highlight the value of biosensor-based approaches in aquaculture and affirm the critical role of the Qingtian carp in sustainable integrated rice–fish systems. The domestication of common carp in rice paddies (5–20 cm depth) is challenging, as the fish must withstand drastic fluctuations in temperature and dissolved oxygen, restricted movement, and bird predation without the option of diving. The effects of stress responses in different species of carp in shallow-water environments remain poorly understood, particularly with fluctuating water levels where real-time monitoring is challenging. This study employed a glucose biosensor system enabling real-time monitoring, together with biochemical analysis techniques capable of evaluating multiple physiological indicators, to investigate shallow-water adaptation in Qingtian paddy field carp and Xingguo red carp. Our results quantitatively reveal, for the first time, the differing physiological stress thresholds of the two carp strains under shallow water. The Qingtian paddy field carp exhibited a higher tolerance to shallow water and showed faster recovery from prolonged stress. Furthermore, the total cholesterol and triglyceride contents of Qingtian paddy field carp gradually increased with prolonged shallow-water stress, reflecting the activation of lipid metabolic pathways. These findings highlight the advantages of biosensor technology in aquatic stress research and a strong support of the core element of paddy domesticated carp in the Globally Important Agricultural Heritage Systems.
研究领域:
鱼类生理学
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水产养殖学
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环境应激生物学
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代谢生理学
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生物传感器技术
·
可持续农业系统
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产品关联
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2021
·
IF 3.228
Hui Li,Guilan Di,Yi Zhang,Junping Liang,Xianfeng Wang,Zhenshan Xu,Xianghui Kong
MicroRNAs (miRNAs) play a critical role in regulating the response of animals exposed to heavy metal stress. As a globally dispersed heavy metal in aquatic ecosystems , cadmium (Cd) is highly toxic to many aquatic species . However, little is known about the miRNA response to Cd stress in fish. To investigate the regulatory effect of miRNAs in response to Cd, common carp ( Cyprinus carpio ) were exposed to Cd 2+ -containing water (0.005 mg/L, 0.05 mg/L, 0.5 mg/L) for 30 days. After exposure, Cd 2+ contents were significantly higher in the kidneys of C. carpio compared to other tissues, when exposed to 0.5 mg/L Cd 2+ . Hematoxylin and eosin staining images revealed that elevated Cd induced inflammatory damage in the kidneys of C.carpio . Further, miRNA sequencing revealed nine differentially expressed miRNAs (miR-217, miR-205 and seven novel miRNAs) in the kidneys, between 0.5 mg/L Cd 2+ exposure and control groups. Potential target mRNAs of miRNAs suggest that miR-217 is involved in immunotoxicity . miR-217 agomir was intraperitoneally administered to C. carpio and RT-PCR revealed that the expression of IL-8 and SIRT1 decreased, while TLR-4, TRAF6 , NF-kB, TNF-α, IL-1β, and TGF-β increased in the kidneys of C.carpio . Additionally, the expression of SIRT1 decreased, while the expression of other mRNAs increased in kidneys of C. carpio exposed to Cd. According to mRNAs expression in the agomir and Cd treatment, miRNAs inhibit the expressions of target mRNAs. These results demonstrate that miR-217 via SIRT1 plays a regulatory role in the immunotoxicity of Cd to C. carpio .
研究领域:
环境毒理学
·
分子生物学
·
水生生态学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 12
·e70783
·
IF 3.4
Xiao Liu,Shuguang Si,Ting Wang,Danfeng Huang,Xi Meng,Ke Li,Yunfang Qian
Excessive consumption of salt poses a threat to human health. While salt is essential for developing quality characteristics in surimi, directly reducing salt content negatively affects surimi products quality. This study investigated, for the first time, the effects of different flaxseed gum (FG) additions (0, 0.1%, 0.2%, 0.3%, 0.4%) on low-salt carp surimi (0.9% NaCl), aiming to reduce salt content while maintaining product quality. The results demonstrated that compared to the low-salt group, 0.3% FG incorporation enhanced gel strength from 22 to 33 N, improved water holding capacity (WHC) from 66% to 73%, and reduced weight loss from 12% to 9% ( p < 0.05). A significant increase in immobile water proportion was observed along with a corresponding decrease in free water proportion ( P < 0.05). The enhanced storage modulus (G') indicated improved gel elasticity. FG addition promoted secondary structure transition from α-helix to β-turn. Microstructural analysis revealed that low-salt surimi became increasingly homogeneous with FG content increasing from 0.1% to 0.4%, with the optimal 0.3% FG concentration showing the lowest porosity (31.929%). These findings support the utilization of FG in low-salt freshwater fish surimi production.
研究领域:
食品科学与技术
·
水产品加工
·
功能性食品成分
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Zhao Jia,Zhao Hongxia,Zhong Zhihui,Bekele Tadiyose Girma,Wan Huihui,Sun Yuming,Li Xintong,Zhang Xiaonuo,Li Zhansheng
Tetrabromobisphenol A bis (allyl ether) (TBBPA-BAE) is an extensively used brominated flame retardant, which has raised considerable concern because of its neurotoxic and endocrine disruption effects on aquatic organisms. However, previous studies mainly focused on the parent compound before modification, tetrabromobisphenol A (TBBPA), and little information is available about the bioconcentration and biotransformation of TBBPA derivatives in fish. In this study, we investigated the tissue-specific uptake, elimination kinetic, and biotransformation of TBBPA-BAE in common carp ( Cyprinus carpio ). The fish were exposed to TBBPA-BAE at environmentally relevant concentrations (20 μg·L −1 ) for 28 days, followed by 14 days of depuration. The results showed TBBPA-BAE could rapidly accumulate in common carp. Among the seven tissues studied, the highest concentrations of TBBPA-BAE were observed in the liver (6.00 μg·g −1 wet weight [ww]) on day 24, while the longest residence time was observed in the kidney ( t 1 /2 values of 18.7 days). Biotransformation of TBBPA-BAE was documented in the in vivo experiments, and 14 different phase I and phase II metabolites were identified in the liver. These findings suggest the biotransformation products of TBBPA-BAE should be considered for a comprehensive risk evaluation. Graphical abstract
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Zhijuan Nie,Xiaotong Xu,Yanhan Ji,Ping Gao,Sihan Zhou,Yi Sun,Jian He,Lanmei Wang,Nailing Shao,Yu Shi,Xu Liu,Pao Xu,Baozhan Wang
Rice-fish co-culture systems, as compared to fish monoculture, offer a sustainable agriculture model that balances productivity with environmental conservation. While the important role of gut microbiome in fish growth and health is increasingly recognized, the impact of different farming practices on fish quality and their gut microbiome remains insufficiently understood. To address this gap, we conducted a comprehensive survey of 292 carps ( Cyprinus carpio ) raised in four rice-fish co-culture systems (RF) across China, comparing them with site-matched pond systems (PF). Through a combination of amplicon sequencing, metabolomic analysis, and bioinformatics, we investigated changes in carp quality, as well as alterations in gut microbiota and metabolites, and explored the potential links between them. Our results reveal that RF system significantly improve carp quality and alter gut metabolite profiles. Further analysis of gut microbial subgroup showed that RF co-culture induced homogenization in the community structure of rare microbial taxa. Ecological network analysis indicated that RF enhances the complexity and stability of microbial networks, particularly among rare taxa, which play a previously underestimated role in gut ecosystems. Community assembly analysis suggested that deterministic processes more strongly shape the microbial communities in carps from RF system, promoting habitat-dependent selection. By developing a bi-network model to explore host-microbe interactions, we identified key microbial taxa, such as Cetobacterium , that are closely associated with beneficial traits in carp quality. Overall, this study highlights the potential of Rice-fish co-culture to foster beneficial host-microbe interactions, providing a microbial ecological perspective on understanding aquaculture safety and sustainability.
研究领域:
水产养殖
·
微生物生态学
·
代谢组学
·
环境科学
·
渔业
·
生物信息学
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
·
IF 4.3
Yimin Li,Chunnuan Zhang,Zihao Meng,Mengkang You,Huajuan Shi
TBBPA, a ubiquitously distributed brominated flame retardant, poses significant ecological risks due to its bioaccumulative potential and toxicity. Previous investigations have predominantly focused on untargeted metabolomics and lipidomics to characterize the effects of TBBPA on metabolic end products. However, limited data are available regarding the mechanisms underlying TBBPA-induced disruption of lipid metabolism in aquatic organisms. This study systematically investigated the dose-dependent effects of TBBPA on lipid metabolism in common carp through integrated biochemical, histochemical evaluations, and transcriptomic profiling. Fish were exposed to environmentally relevant TBBPA concentrations (0, 0.005, 0.05, and 0.5 mg/L) for a 14-day duration. Serum biochemical analyses revealed dose-dependent hyperlipidemia, manifested by elevated TG and T-CHO levels, suppressed SOD and CAT activities, and increased MDA levels. Oil Red O staining demonstrated hepatic lipid droplet accumulation despite no significant ( P > 0.05) alterations in intrahepatic TG/T-CHO content, with the 0.5 mg/L group exhibited the most severe histopathological phenotype. Transcriptomic profiling identified the AMPK and PPAR signaling pathways as pivotal regulators of TBBPA-induced metabolic dysregulation. Under low-dose TBBPA exposure (0.05 mg/L), lipid accumulation triggered compensatory activation of the AMPK / PPAR-α / CPT1 axis, enhancing fatty acid β-oxidation to partially counteract lipid deposition. Conversely, high-dose TBBPA (0.5 mg/L) disrupted lipid metabolism through coordinated upregulation of genes associated with lipid synthesis, transport, and storage, culminating in pathological lipid deposition. This study provides critical insights into the molecular cascades underlying TBBPA-induced metabolic dysregulation in aquatic species, highlights the dose-dependent disruption of lipid homeostasis.
研究领域:
环境毒理学
·
水生毒理学
·
分子生物学
·
生物化学
·
转录组学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·5623889
·
IF 3.9
Xianglin Cao,Rongjie Yuan,Yi Guo,Mengtao Jia,Yinyin Wei,Jiameng zhou,Han Cui,Baohua Li,Jianjun Chen
L-Carnitine is widely recognized for its involvement in lipid metabolism, but its effects on muscle quality and gut health in carp have not been well studied. The research aimed to investigate how L-carnitine influences muscle quality and intestinal health in high-fat-fed carp. The study was separated into four groups that received either the standard diet, a high-fat diet (HFD), or a HFD supplemented with 500 mg/kg L-carnitine (LLC), or a HFD supplemented with 1000 mg/kg L-carnitine (HLC) for 56 days. L-Carnitine was found to significantly reduce blood lipid levels. In addition, L-carnitine increased the crude protein content and decreased the crude fat content of high-fat-fed carp muscle while improving muscle fiber morphology and muscle quality. L-Carnitine increased the expression of genes related to intestinal tight junction proteins ( claudin-2 , occludin , and zo-1 ), improved the expression of genes related to intestinal inflammation, and enhanced the physical barrier function and organization of the intestine. Analysis of intestinal flora and intestinal metabolites showed that L-carnitine increased the diversity of the intestinal flora, increased the abundance of Cetobacterium , and influenced intestinal levels of bile acids, arachidonic acid, and tryptophan-related metabolites. In conclusion, supplementation with 1000 mg/kg L-carnitine improved muscle quality and intestinal health significantly in high-fat-fed carp by regulating muscle nutrient metabolism and intestinal flora.
研究领域:
水产养殖
·
营养学
·
生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）