NEW JOURNAL OF CHEMISTRY
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2025
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IF 2.5
Yuying Jia,Xiangyu Cai,Xuepeng Wang,Hongyan Shan,Yanfu Huan
Accurate detection of Cys/Hcy levels is critical for evaluating human health conditions. In this study, we developed an innovative fluorescence/colorimetric dual-mode sensing system to detect Cys/Hcy by dynamically adjusting the level of oxTMB. The system utilizes Au@PtNPs with peroxidase-like activity to catalyze the oxidation of colorless 3,3,5,5- tetramethylbenzidine (TMB) to blue oxTMB, and TAPB@DMTP COF, a fluorescent nanomaterial with abundant spatial pores, could be used as a carrier for oxTMB, and the two were made fluorescence quenching due to the fluorescence inner filter effect. The addition of Cys/Hcy allowed the originally dispersed Au@PtNPs to aggregate to inhibit their peroxidase-like activity, thus indirectly regulating the oxTMB content. At this point, the absorbance signal decreased and fluorescence was restored. To this end, we have developed a special fluorescence/colorimetric dual-mode sensing system for the detection of Cys/Hcy. The detection limits of the fluorescence and colorimetric methods for Cys/Hcy were determined to be 0.788 nM (Cys), 0.663 nM (Hcy), and 1.795 nM (Cys), 1.886 nM (Hcy), respectively. The sensing system was also evaluated for its ability to withstand interferences from 12 anions and 13 amino acids, demonstrating excellent unti-interference and selectivity. The recoveries of the two methods for detecting Cys/Hcy in fetal bovine serum were found to be 98.9%-114.3% with RSDs as low as 0.52% (fluorescence) and 0.92% (colorimetric), respectively.
研究领域:
生物化学
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分析化学
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Qinghua Hu,Changmin Yu,Xitao Xia,Fang Zeng,Shuizhu Wu
Biothiols like Cys, Hcy and GSH play important roles in various physiological and pathological processes, and it is still challenging to simultaneously and discriminatively detect GSH and Cys/Hcy due to their similar structures and reaction activities. Hence, it would be highly desirable to design a fluorescent probe for simultaneously discriminating GSH and Cys/Hcy in biological samples with no spectral crosstalk , few interferences and rapid response. Herein, through coupling two fluorophores with biothiol-sensitive linker, we developed a fluorescent probe , which has two biothiol-responsive and distinctly-separated emissions via independent visible-light excitations, for simultaneously discriminating GSH and Cys/Hcy with near-infrared and green emissions. The probe is operable in human serum samples, thus holding promise for diagnostic-related applications. Moreover, the probe shows quite good properties. First, it exhibits a rapid response (within a few minutes) with highly selective and sensitive detection for GSH and Cys/Hcy. Second, it offers an apparent colorimetric and two fluorescence emission signals without spectral crosstalk . Third, it shows low cytotoxicity. Therefore, it would provide a useful method for further elucidating the roles of biothiols as well as for conducting pathological analysis for diseases involving biothiols.
研究领域:
生物化学
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分析化学
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医学诊断
✓ 已验证
DOI
高影响因子
Nanoscale
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2020
·Vol. 12
·期 24
·13010-13016
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IF 6.895
Qiaoling Liu,Xiuyun Niu,Yan Zhang,Ying Zhao,Kaixin Xie,Boru Yang,Qing He,Shiyou Lv,Lin Li
The abnormal concentrations of both biothiols and pH in lysosomes are seriously related to many major diseases, such as Parkinson's and Alzheimer's diseases. Up to now, there are few reports that clearly illustrate the relationship between lysosomal pH and biothiols via fluorescence assay. Herein, novel carbon dots (Scy-CDs) are prepared with good water dispersibility and excellent photostability, and a large Stokes shift of 106 nm is exhibited under an excitation wavelength of 450 nm. The remarkable pH-dependent behavior of Scy-CDs is presented with the fluorescence quenching based on the donor-excited photoinduced electron transfer (d-PET) process. The pKa value is 5.30, which is in good agreement with the range of the normal and abnormal lysosomal pH. Upon the addition of cysteine (Cys) or homocysteine (Hcy), the d-PET process is effectively inhibited with fluorescence recovery totally. The significant co-localization of Scy-CDs with Lyso-Tracker Deep Red in HEp-2 cells and the Pearson correlation coefficient 0.88 strongly suggest that the Scy-CDs can target lysosomal pH and Cys/Hcy in living cells.
研究领域:
细胞生物学
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医学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
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2019
·Vol. 9
·期 14
·7955-7960
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IF 3.049
Hui Zhang,Lizhen Xu,Wenxiu Li,Wenqiang Chen,Qi Xiao,Jun Huang,Chusheng Huang,Jiarong Sheng,Xiangzhi Song
A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.
研究领域:
分析化学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2018
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IF 6.042
Kangming Xiong,Fangjun Huo,Jianbin Chao,Yongbin Zhang,Caixia Yin
Although some progress has been made in distinguishing the detection of biothiols, NIR biothiol fluorescent probes for simultaneously distinguishing detection of Cys, Hcy, and GSH in vivo have not been reported. The design of these probes involves the introduction of NIR fluorophores and multiple binding sites; thus, the integrated design of probes remains a challenge. Although Cys, Hcy, and GSH have common functional groups, a sulfydryl group and an amino group, due to their differences in spatial structure, they may react with multiple binding site probes to produce different reaction products in different bonding mechanisms, resulting in different colors and fluorescent signal changes of the system. Therefore, multiple binding site fluorescent probes can realize their discrimination detection. For an NIR fluorescent probe, it is easier to realize in vivo imaging to promote the research of biothiols in clinical diagnosis. In our work, not only were multiple binding sites constructed in the compound but also NIR fluorophores were introduced. This enables the probe to not only efficiently distinguish detection of Cys/Hcy and GSH but also achieve fluorescence imaging in vivo. We believe this result is a milestone in the discrimination detection of biothiols.
研究领域:
生物化学
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医学诊断
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分子探针研究
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
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2025
·
IF 14.1
Ting Yu,Yang Li,Jing Li,Yabing Gan,Zhengze Long,Yun Deng,Youyu Zhang,Haitao Li,Peng Yin,Shouzhuo Yao
Adenosine triphosphate (ATP) is a critical intracellular energy currency that plays a key role in various cellular processes and is closely associated with numerous diseases. Similarly, biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are integral to many physiological and pathological processes due to their strong redox properties. Simultaneous discrimination and detection of ATP and biothiols offer valuable insights into the pathogenesis of conditions such as epilepsy and liver injury. This study introduces the first fluorescent probe, BCR , designed for multifunctional detection of ATP, GSH, Hcy, and Cys. With outstanding optical properties, excellent biocompatibility, high selectivity, and superior sensitivity, probe BCR enables effective imaging of ATP and biothiol dynamics in vivo. Moreover, probe BCR successfully visualizes changes in ATP, GSH, Hcy, and Cys levels in a PTZ-induced epileptic zebrafish model and an APAP-induced mouse liver injury tissue section model. These findings underscore the significant potential of probe BCR for early disease diagnosis and therapeutic applications.
研究领域:
生物化学
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医学诊断
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药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Yang Li,Jing Li,Ting Yu,Hongyi Cai,Youyu Zhang,Haitao Li,Huijun Zhou,Peng Yin
The Mercury (II) ion (Hg²⁺) is a toxic heavy metal that threatens biological systems by inducing oxidative stress and disrupting the redox balance. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are critical in maintaining redox homeostasis and are implicated in numerous physiological and pathological processes. Understanding the complex interactions between Hg²⁺ and biothiols requires molecular tools capable of simultaneous detection. Herein, we report a novel multi-signal fluorescent probe, DPC , designed with four specific recognition sites and rhodamine and coumarin fluorophores. The DPC probe enables the selective and sensitive differentiation of Mercury (II) ion (Hg²⁺), cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), with distinct fluorescence signals. DPC can detect mercury (II) ions (Hg²⁺) in water samples with a high recovery rate ranging from 90.44 % to 112.27 %. DPC was also successfully applied for real-time imaging of these species in living cells and zebrafish, revealing that Hg²⁺ induces oxidative stress, reducing cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) levels. Furthermore, detoxifying agents such as sodium selenite (Na₂SeO₃), 2,3-dimercaptopropan-1-ol (DMPC), and L-selenocysteine (Sec) restored biothiol levels, counteracting Mercury (II) ion (Hg²⁺) toxicity. These results highlight the exceptional performance of DPC for the simultaneous imaging of Mercury (II) ions (Hg²⁺) and biothiols, providing insights into their interactions and proposing a chemobiological framework for studying heavy metal toxicity and developing detoxification strategies.
研究领域:
生物化学
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毒理学
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分析化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaojie Ren,Huihui Tian,Liu Yang,Long He,Yani Geng,Xingjiang Liu,Xiangzhi Song
Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are of great importance in many physiological and pathological processes. Due to their similar structure and reactivity of these three biothiols, it is a great challenge to simultaneously distinguish one from another. By virtue of two different fluorescence signals, nitrobenzofurazan ( NBD ) has been proved to be an effective sensing functional group as well as a green fluorophore precursor to construct fluorescent probes for simultaneous discrimination of Cys/Hcy and GSH. However, most of the reported NBD-based fluorescent probes suffered from a poor water-solubility, a slow response, double-wavelength excitation or two interfering fluorescence signals (narrow wavelength separation in λ max Em and/or big difference in Φ Fl ). In this study, we developed a fluorescent probe, MCQ-O-NBD , for simultaneous discrimination of Cys/Hcy and GSH using two matched fluorescent signals (well separated red and green fluorescence and comparable Φ Fl ), which could function in a pure aqueous media with a rapid response under a single-wavelength excitation. This probe exhibited high sensitivity and good selectivity as well as low detection limit, and had been successfully applied to visualize and differentiate Cys/Hcy and GSH in living cells.
研究领域:
生物化学
·
医学检测
·
荧光成像
✓ 已验证
DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2020
·
IF 3.28
Jin Bai,Xin Yang,Ying Qian
In this work, a unique ratiometric and NIR fluorescent probe , Hecy-NBD, was designed and synthesized to detect cysteine/homocysteine/glutathione in aqueous solution. Hemicyanine skeleton was chosen as the major fluorophore and nitro benzoxadiazole (NBD) chromophore was selected as the recognition unit to construct a ratiometric fluorescent probe. The fluorescence of probe Hecy-NBD was very weak due to the introduction of quenching group NBD . Upon the addition of 100 μM GSH to 10 μM Hecy-NBD, the fluorescence intensity at 702 nm was very strong with the releasing of compound Hecy-OH. While upon the addition of 100 μM Cys or 100 μM Hcy, probe exhibited distinct fluorescence enhancement at 540 nm and 702 nm, which resulted from the formation of compound NBD-Cys and NBD-Hcy through the 5/6-membered cyclic transition and the fluorescence releasing of compound Hecy-OH. The proposed mechanism based on S N Ar reaction and molecular rearrangement, which were confirmed by HRMS spectra. The detection limit of these three biothiols was also calculated (GSH, 50 nM; Cys, 81.0 nM; Hcy, 130 nM). Moreover, probe Hecy-NBD could function well at wide pH conditions from 4 to 10. Besides that, probe Hecy-NBD with low cell toxicity had been successfully applied to dual-channel fluorescence imaging in living cells. Considering the good characteristics, Hecy-NBD had great potential to detect biothiols-associated diseases in the future.
研究领域:
生物化学
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医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Qian Wang,Wenting Zhang,Mingzhe Cai,Yafei Ma,Ajuan Yu,Sheng Chen,Shusheng Zhang
The reactive sulfur species (RSS), in particular Cys, Hcy , GSH, and H 2 S, play an essential role in lots of critical physiological and pathological processes. To elucidate their complex interrelationships in signaling and metabolic pathways, suitable molecular tools to simultaneously distinguish Cys, Hcy , GSH, and H 2 S are urgently needed. Herein, we developed a novel cascade reaction-assisted trinal-site fluorescent probe BCS by connecting three functional fragments for simultaneously distinguishing Cys, Hcy , GSH, and H 2 S. The probe BCS exhibited red fluorescence in the absence of the four biological RSS. However, with the attendance of Cys, Hcy , GSH, and H 2 S, the probe showed different fluorescence patterns, including blue, blue/red, green/red, and no fluorescence, respectively. More importantly, based on the different RSS-induced fluorescence patterns and multi-color fluorescence imaging, probe BCS was used to discriminate and visualize all four biological RSS in both HepG2 cells and zebrafish. It is expected that probe BCS will have applications in the diagnosis and research of RSS-related diseases in the future.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Jie Zhang,Haishuang Jia,Wenfang Liu,Jun Wang,Dawei Fang
Cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H 2 S) are essential active substance in human body. The identification and detection of biothiols and H 2 S are of great significance for the early diagnosis of certain diseases. In this paper, a new dual excitation and dual emission fluorescent probe , carbon quantum dot-O-7-nitrobenzo-2-oxa-1,3-diazole (CQDs-O-NBD), for the detection of biothiols and H 2 S was synthesized. The carbon quantum dots (CQDs-OH) (λ ex/em maxima = 377 nm/467 nm) were synthesized by a microwave-assisted method using hydroquinone as the carbon source. Through the reaction of the phenolic hydroxyl group of the carbon quantum dots and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), the CQDs-O-NBD was synthesized. Due to the static quenching (SQ) and the photo-induced electron transfer (PET) from CQDs to NBD, the fluorescence of the CQDs-O-NBD is very weak. After adding biothiols and H 2 S, the ether bond of the CQDs-O-NBD was broken through nucleophilic substitution reaction , which led to the release of the CQDs-OH (channel 1 is turned on) and generation of a new compound NBD-thiols (channel 2). The CQDs-O-NBD as a probe shows the high selectivity and sensitivity for Cys/Hcy/GSH/NaHS (LOD of 0.24 μM/0.21 μM/0.19 μM/0.18 μM) over other aminos and ions by channel 1. The new formed compounds from NBD and Cys/Hcy have strong fluorescence under 470 nm wavelength light excitation (channel 2 is turned on), while the formed compounds from NBD and GSH/NaHS have no fluorescence (no channel 2), so the CQDs-O-NBD can discriminate Cys/Hcy (LOD: 0.07 μM/0.06 μM) from GSH/NaHS. In addition, the CQDs-O-NBD shows low toxicity and can be applied to the detection of Cys in fetal bovine serum and imaging in living cells.
研究领域:
分析化学
·
生物化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）