CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Xiaoting Li,Cheng Qian,Yonghui Tian,Naizhi Yao,Yixiang Duan,Zhijun Huang
In this work, Pt-Ru bimetallic nanoclusters (Pt-Ru NCs) were prepared by a simple one-pot hydrothermal method. The as-prepared Pt-Ru NCs has extremely high peroxidase-like activity, showing a specific activity of 115.36 U/mg far superior to Pt NCs and many reported single-atom nanozymes. This can be attributed to the various microstructures effective for catalysis and unique electron transfer characteristics leading to active hydroxyl radicals ( OH) activation as revealed by density functional theory. By using the Pt-Ru NCs as a substitute for natural horseradish peroxidaseand when cross-linking with antibody as a catalytic label, the colorimetric enhanced lateral flow immunoassay (LFIA) for D-dimer detection was established. As a result, with only 20 s of additional coloration, the visual sensitivity can be improved by about 100 times to 1 ng/mL, while maintaining good specificity and anti-interference. In general, the excellent performance indicates that the Pt-Ru NCs have great potential in colorimetric detection, which is worthy of further promotion.
研究领域:
生物化学
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医学诊断
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纳米材料
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
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IF 8.758
Haiping Wu,Min Zhao,Jia Li,Xiaoyan Zhou,Tiantian Yang,Dezhang Zhao,Ping Liu,Huangxian Ju,Wei Cheng,Shijia Ding
Most of chemiluminescence (CL) substrates exhibit the flash-type light emission. Therefore, the long-lasting CL system is always the crown in the field of CL-based analysis methodology. In this work, we constructed a Dox-ABEI chimeric magnetic DNA hydrogel (MDH) as a novel protease-free long-lasting CL reaction system. The functional MDH can transform flash-type ABEI/H2O2/CO2+ reaction into a glow-type CL system because of its block effect on delaying the diffusion rate of co-reactants, making the CL reaction gradually occur. More importantly, the functional MDH possessed the advantages of biocompatibility and controllability and could be well-designed to incorporate different biosensing strategies. Subsequently, we established a functional MDH-based long-lasting CL immunoassay system for ultrasensitive and highly specific detection of d-dimer and fibrin degradation products (FDPs). The designed CL immunoassay can detect d-dimer and FDP down to 53.7 and 31.6 fg/mL, respectively, with a wide line ranging from 100 fg/mL to 100 ng/mL, which was superior to the previously reported CL biosensing strategies. Moreover, benefiting from the magnetic separation of MDH and excellent CL performance, the developed immunoassaying method was successfully applied in the detection of clinical samples, which showed a close correlation with clinical reference technology. Thus, this functional MDH proved to be an excellent long-lasting CL system and a potential technical platform for clinical bioanalysis applications.
研究领域:
分析化学
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生物化学
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生物技术
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临床化学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2020
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IF 7.242
Ying Wang,Xiaowen Xu,Xuan Zhao,Zongning Yin
Pathological thrombosis within a vessel hampers blood flow and is the mainspring of numerous fatal cardiovascular complications. In order to specifically image and dissolve a thrombus, we rationally designed a functionalized polymeric hybrid micelle (PHM) system self-assembled from amphiphilic polycaprolactone-polyethylenimine (PCL-PEI) and polycaprolactone-polyethylene glycol (PCL-PEG). Based on a biological component of thrombi, activated coagulation factor XIII (FXIIIa), which is responsible for fibrin crosslinking, we further developed FXIIIa-targeted near infrared imaging and thrombolytic nanoparticles , termed IR780/FPHM/LK NPs, through chemical conjugation of peptides to the system. In a ferric chloride (FeCl 3 )-induced mouse carotid thrombosis model, IR780/FPHM/LK NPs specifically targeted the thrombus and significantly enhanced the photoacoustic signal for an accurate diagnosis. When loaded with the fibrinolytic drug lumbrokinase (LK), FPHM remarkably dissociated the thrombus accompanied by an increase in the d -dimer level, a fibrin degradation product , and alleviation of fatal nonspecific hemorrhagic risk. Given its thrombus-specific imaging along with potent therapeutic activities, IR780/FPHM/LK NPs hold promise for developing nanotheranostic agents in preclinical thrombotic vascular disease models.
研究领域:
纳米技术
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诊疗学
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心血管疾病
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Nutrients
·
2023
·Vol. 15
·期 6
·1522
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IF 5.9
Jiyong Yin,Jiangping Niu,Junsheng Huo,Jing Sun,Jian Huang,Chaoqun Sun
We attempted to construct and evaluate a novel detection method to realize simultaneous detection based on a multiplex liquid protein chip technique for nine nutrition-and-health-related protein markers to meet the requirement of an accurate, simultaneous and comprehensive analysis of the proteomics of nutrition and health. The lower limits of detection, biological limits of detection and regression equations of serum ferritin (SF), soluble transferrin receptor (sTfR), c-reactive protein (CRP), retinol-binding protein4 (RBP4), apolipoprotein B (ApoB), alpha-fetoprotein (AFP), prealbumin (PA), carcino-embryonic antigen (CEA) and D-Dimmer (D-D) were determined after a series of optimal experiments. Then, the results of the methodological evaluation for this novel method indicated that the accuracies were between 70.12% and 127.07%, the within-run precisions were between 0.85% and 7.31%, the between-run precisions were between 3.53% and 19.07%, the correlation coefficients between this method and other methods were above 0.504 (p< 0.05), and the direct bilirubin (DBIL) of low concentration and the indirect bilirubin (IBIL) of high concentration could not interfere with the detected results of nine indicators. The novel multiplex detection method, which can increase accuracy and improve the ability of comprehensive analysis, can basically meet the requirement of detection and the diagnosis of the proteomics of nutrition and health.Keywords:liquid protein chip;nutrition and health;protein marker;multiplex detection
研究领域:
蛋白质组学
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临床生化
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营养科学
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）