ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Kairen Zhao,Lin Hou,Feng Li,Yuanyuan Xue,Yan Jin,Baoxin Li
The rise of antibiotic-resistant bacteria poses a severe global health threat, driving the urgent need for alternative antimicrobial strategies. Nanomaterials, particularly silicon quantum dots (SiQDs), offer promising potential due to their multimechanistic antibacterial action and lower risk of inducing resistance. However, the practical application of conventional SiQDs is often constrained by a limited antibacterial efficacy. In this work, chiral SiQDs (D-SiQDs and L-SiQDs) were synthesized via a one-step polymerization method using (3-aminopropyl)trimethoxysilane (APTMS) and d- or l-cysteine as raw materials. Their antibacterial activity was evaluated against Gram-positive S. aureus and Gram-negative E. coli. D-SiQDs exhibited antibacterial activity against S. aureus and E. coli, achieving sterilization rates of 95% at concentrations of 50 μg/mL and 250 μg/mL, respectively, significantly outperforming achiral SiQDs (1 mg/mL and 2 mg/mL). Thus, the chiral introduction significantly enhanced the antibacterial activity of SiQDs. Mechanistic studies revealed that D-SiQDs caused more severe membrane damage, nucleic acid leakage, and ATP depletion in S. aureus. Furthermore, when applied to polypropylene fabrics, D-SiQDs confer excellent antibacterial properties while showing minimal cytotoxicity. This work highlights the significant role of chirality in enhancing the antibacterial performance of nanomaterials and presents chiral SiQDs as a highly promising, biocompatible alternative to conventional antibiotics for anti-infective applications.
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LANGMUIR
·
2025
·
IF 3.9
Huimin Wang,Guangxin Wang,Ruoxi Liu,Jin Yong Lee,Shufeng Liu,Yingqi Tang,Jing Yu
The development of highly efficient chiral recognition systems by simple and cost-effective means is critical to advance the precise application of chiral molecules. Herein, a novel electrochemiluminescence (ECL) enantioselective recognition platform was constructed based on l-histidine-functionalized zeolitic imidazolate frameworks (l-His-ZIF8). Detailed characterization verified the successful preparation of the chiral l-His-ZIF8 material. It was then applied to the chiral discrimination of six amino acids: cysteine (Cys), tryptophan (Trp), phenylalanine (Phe), glutamic acid (Glu), histidine (His), and proline (Pro). Among them, the system employing Ru(bpy)32+ as the luminophore demonstrated the most efficient chiral recognition of d-Cys over l-Cys, as evidenced by a distinct ECL signal ratio (ECLd-Cys/ECLl-Cys) of 3.3 at 1 mM. A detection limit of approximately 0.193 mM was achieved for Cys enantiomers. Conversely, an inverse chiral recognition was observed for the tryptophan (Trp)/sodium tetraphenylborate (TPB) system, resulting in a stronger ECL response for l-Trp over that for d-Trp (ECLl-Trp/ECLd-Trp = 2.4 at 1 mM). Theoretical calculations indicate that the excellent ECL enantioselectivity stems from the preferential binding of the “host” l-His-ZIF8 with specific “guest” enantiomers (d-Cys or l-Trp) via stronger intermolecular interactions. This preferential binding leads to the formation of more stable diastereoisomers that facilitate electrooxidation of more “guests” to generate a stronger luminescent signal than its isomer. This study establishes a novel strategy for electrochemiluminescent enantiomer discrimination using chiral site-engineered metal–organic frameworks (MOFs) and opens new avenues for the future of chiral sensing.
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Food Bioscience
·
2026
·
IF 6.2
Mengmeng Ji,Jinwei Li,Liuping Fan,Liang Wang,Shengquan Huang
Pseudomonas aeruginosa , a significant opportunistic pathogen, poses a serious threat to food safety due to its antibiotic resistance and strong biofilm-forming ability. In this study, quercetagetin (Que) was identified as a novel quorum sensing (QS) inhibitor. Reporter strain assays, molecular docking, and dynamic simulation indicated that Que acts as an antagonist of the QS receptors. Que attenuated QS-regulated virulence phenotypes in a dose-dependent manner but showed insufficient antibiofilm activity ( p >0.05) at sub-minimum inhibitory concentration (sub-MIC) levels. In contrast, the combination of 0.5 mmol/L Que with 20 mmol/L D-leucine or D-tryptophan exhibited additive effects on planktonic cells, reducing its survival by 2.19–3.97 log compared to the control. For biofilm cells, the combined treatment potentiated the reduction in cell density by 0.48–1.42 log. Notably, these effects were strain-dependent but were equal to or outperformed those of the ε-polylysine (ε-PL) control. Mechanistically, this enhancement was driven by the disruption of biofilm matrix and cell membrane integrity. This was evidenced by the greater suppression of Acyl-homoserine lactones (AHLs) synthesis and virulence phenotypes, alongside substantial reductions in biofilm biomass and roughness and increased in membrane permeability compared to Que. Furthermore, the combination resulted in repression of key enzymes activities (Na + -K + -ATPase and succinate dehydrogenase) by 29%–38%. These changes indicated a promising strategy to control P. aeruginosa and enhance food safety.
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MATERIALS CHEMISTRY AND PHYSICS
·
2022
·
IF 4.778
Dan Zhao,Mengyu Xu,Kang Dai,Huan Liu,Yan Jiao,Xincai Xiao
Chirality is one of the most widespread properties in nature. After the successful application of non-optical active carbon dots (CDs) in the fields of detection, imaging, and antibacterial, the researchers turned more attention to the preparation and applications of chiral CDs. This paper reports the preparation of several chiral CDs with chiral amino acids as carbon sources through two different methods (one-step hydrothermal route and two-step coupling route), and further explores the difference in antibacterial abilities of these chiral CDs prepared by different carbon sources and synthesis methods. The experiments results showed that chiral D/LGU which are prepared through one-step method and with glutamic acid (Glu), are superior to those prepared by two-step method (NLDU/NDGU), or those prepared with cysteine as raw material (D/LCS). For D/LCS, the antibacterial ability of the L type is superior to that of the D type, proving that the synthesis method and the chirality of raw material are both important in the antibacterial behavior of prepared CDs. The chirality and optical properties of prepared CDs have been characterized by UV–vis spectrophotometer , fluorescence spectrology, and circular dichroism. Through MurA enzyme inhibition tests, SEM, and molecular docking design, the antibacterial mechanism of chiral CDs has been investigated. The research provides a sound foundation for the preparation, characterization, and application of chiral CDs, as well as the novel targeting antibacterial applications.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Hongying Li,Jiale Zhang,Lingling Jiang,Ruo Yuan,Xia Yang
The identification of enantiomers is of great importance in chiral separations and medicinal chemistry . While Surface-enhanced Raman spectroscopy (SERS) is a technique that provides vibrational fingerprints of analytes . The enantiomers identification relies on the SERS difference between left and right-handed circularly polarized light or additional selectors for indirect distinction. In this work, Au–Ag core shell nanobipyramid (L/ [email protected] BPs) were synthesized guiding by chiral encoder of L/D-cysteine. L/ [email protected] BPs produced plasmon-induced circular dichroism signals in the plasmon resonance absorption band , which can be tuned by modulation the amount of cysteine. Moreover, the chiral anisotropy factor of L/ [email protected] BPs at 532 nm can reach 5.11 × 10 −3 . Due to the selective resonance coupling between L/ [email protected] BPs and different enantiomers, L/ [email protected] BPs were further used as SERS substrates for efficient discrimination of biologically relevant small molecules . Chiral [email protected] BPs display the potential for chiral drug identification.
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TALANTA
·
2022
·
IF 6.556
Shutong Yang,Liancheng Gu,Fangling Wu,Xinhua Dai,Fuxing Xu,Qiaoyu Li,Xiang Fang,Shaoning Yu,Chuan-Fan Ding
Chiral recognition is of highly interest in the areas of chemistry , pharmaceuticals, and bioscience. An effective strategy of enantiomeric determination of amino acids (AAs) was developed in this work. All 19 natural AAs enantiomers can be easily distinguished by ion mobility-mass spectrometry of the non-covalent complexes of AAs with cyclodextrins (α-CD, β-CD and γ-CD) and Mg 2+ without any chemical derivatization . Differences of the mobilities between the enantiomers’ complexes is from 0.006 to 0.058 V s/cm 2 . In addition, the complex of [β-CD + Phe + Mg] 2+ was selected as an example to study the relative quantification by measuring L/D-Phe at different molar ratio of 10:1 to 1:10 in the μM range, resulting in a good linearity (R 2 > 0.99) and high sensitivity at 2 μM. A DFT calculation was also performed to illustrate the detailed molecular structure of the complexes of CDs , Mg 2+ and D- or L-Phe. Both experiment and theoretical calculation showed that Mg 2+ plays an important role in host/guest interactions, which changed the molecular conformations by non-covalent interaction between Mg 2+ and CDs, and resulted in the different collision cross-sections of the complex ions of CDs, Mg 2+ and D- or L-AAs in the gas phase. This effective and convenient strategy could potentially be utilized in scientific research and industry for routine enantiomeric determination of natural AAs, peptides and some other small chiral biomolecules such as non-natural AAs and carboxylic acid-containing drugs.
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ANALYST
·
2021
·Vol. 146
·期 20
·6245-6251
·
IF 4.616
Qianqian Zhao,Wenrong Cai,Baozhu Yang,Zheng-Zhi Yin,Datong Wu,Yong Kong
Well-dispersed chiral Ag2S quantum dots (Ag2S QDs) were facilely synthesized by using N-acetyl-L-cysteine (NALC) as the chiral ligand and loaded onto nanosheets of two-dimensional (2D) few-layer carbon nitride (C3N4). The resultant nanocomposite (Ag2S QDs/few-layer C3N4) shows enhanced electrochemiluminescence (ECL) while maintaining the chirality of Ag2S QDs, which can be used for the chiral discrimination of the enantiomers of tyrosine (Tyr). Due to the higher affinity of chiral Ag2S QDs toward L-Tyr than toward its enantiomer, the ECL intensity of Ag2S QDs/few-layer C3N4 is significantly decreased after its incubation with L-Tyr, and thus the Tyr enantiomers can be discriminated. The developed ECL chiral sensor exhibits high stability and reproducibility. The universality of the ECL chiral sensor for the discrimination of other chiral amino acids is also studied, and the results indicate that it can work only in the case of chiral aromatic amino acids.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Qingkun Ma,Xiaoxi Man,Chun-Yan Xu,Jian Huo,Chao Qi,Qing Shi,Jun Nan,Jun Zhe Min
Measurement of chiral thiol compounds such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) in human serum plays an important role in the early diagnosis and warning of cardiovascular disease, neurodegenerative disease, and cancer. We developed a novel chiral mass spectrometry derivatization reagent, (R)-(5-(3-isothiocyanatopyrrolidin-1-yl)-5-oxopentyl) triphenylphosphonium (NCS-OTPP), with triphenylphosphine (TPP) as a basic structure carrying a permanent positive charge for the diastereomeric separation of chiral thiol compounds by ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). A novel method was developed for simultaneous determination of three kinds of chiral thiol compounds based on the NCS-OTPP derivatization method. Three kinds of chiral thiol compounds on a YMC Triart C18 (2.0 × 150 mm, 1.9 μm) column with Rs were 1.56–1.68. The protonated precursor to product ion transitions monitored for GSH was m/z 780.16→747.24/473.18, Cys was m/z 594.20→561.18/473.18, and Hcy was m/z 608.21→575.19/473.18. An excellent linearity for all the analytes with correlation coefficients ≥ 0.9995 and suitable precision with inter-day and intra-day coefficients of variation RSDs was 0.83–4.06% and 0.95–3.11%. Satisfactory accuracy with recoveries between 83.73 and 103.35% was observed. The limit of detection (S/N = 3) was 2.4–7.2 fmol. Furthermore, the method was successfully applied to the simultaneous determination of three kinds of free and total thiol compounds in serum from 10 healthy volunteers at normal and stress states.
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ACS Nano
·
2021
·
IF 15.881
Jinglin Shen,Qianwen Xiao,Panpan Sun,Jin Feng,Xia Xin,You Yu,Wei Qi
The self-assembly of chiral metal nanoclusters into supramolecular chiral aggregates is of interest for developing advanced materials. Herein, we manipulated the self-assembly of Au nanoclusters modified by l-/d-cysteine (l-/d-AuNCs) into ordered microstructures featuring enhanced phosphorescence and optical activities. The formation of these aggregates was driven by synergistic effect of coordination and electrostatic interactions assisted by Cd2+/H+. Detailed structural characterization and theoretical studies confirmed that the compact aggregation structures are essential for the emission enhancement and the chirality amplification of l-/d-AuNCs. Interestingly, upon the formation of microflowers, the emission lifetime was prolonged to 3.34 ms with a switch from fluorescence to phosphorescence induced by aurophilic Au(I)···Au(I) interactions and intensive ligand-to-metal charge transfer (LMCT). Moreover, both the CD and photoluminescence (PL) signals of the microflowers exhibited pH-responsiveness. This dual-mode sensitive platform could be developed as a pH sensor with improved accuracy. Additionally, the pH-responsive photoluminescence ON/OFF switch of the microflowers could be employed for reliable information encryption and decryption. This study provides useful ideas for regulating the self-assembly of nanoclusters to generate desired photophysical properties with potential applications.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Qingkun Ma,Chao Qi,Xi-Ling Li,Qing Shi,Chun-Yan Xu,Toufeng Jin,Jun Zhe Min
A high-sensitivity and -selectivity mass spectrometry derivatization reagent, ( R )-(5-(3-isothiocyanatopyrrolidin-1-yl)-5-oxopentyl) triphenylphosphonium (NCS-OTPP), was developed for the enantiomeric separation of chiral thiol compounds as prospectively important diagnostic markers for oxidative stress-related diseases. Complete separation of GSH, DL-Cys, and DL-Hcy was achieved. The parent ions of all derivatives had a fragment of m/z 473.18 and a structure of m/z 75.95 (R-S = C-S-R’), conducive to qualitative and quantitative analysis. Good linear relationships were obtained for all analytes ( R 2 ≥ 0.9995). The intra-day and inter-day precision were 0.82–5.16 % and 1.02–4.18 % in saliva, and 0.81–3.45 % and 0.99–6.47 % in urine, with mean recoveries of 83.31–105.66 % and 84.09–101.11 %, respectively. The limit of detection ( S/N = 3) was 19.20–57.60 nM. Free and total GSH, DL-Cys, and DL-Hcy were detected simultaneously in saliva and urine from 10 volunteers in the normal, stressed, and stable states by UHPLC-Q-Orbitrap HRMS . The thiol compounds were quantitatively related to oxidative stress state changes.
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CORROSION SCIENCE
·
2026
·
IF 10.3
Jingzhi Yang,Mingyue Zhang,Junsen Zhao,Annan Kong,Yu Yang,Yuting Jin,Zhongheng Fu,Yaxin Wang,Lingwei Ma,Dawei Zhang
2D-EI enables concurrent optimization of antibacterial and anticorrosion performance without pre-set weights. • Bridging data-driven approaches with theoretical calculations reveals the key mechanistic drivers of synergistic effects. • Weak molecular interactions may favor the adsorption configurations of inhibitors on metal surfaces. • The optimized pairs with balanced performance exhibit stronger inhibition against microbial corrosion. Developing advanced antibacterial and anticorrosion bifunctional inhibitor formulations represents a promising approach to address microbial corrosion. Up to now, achieving this goal has been hindered by the following challenges: i) the gigantic search space of the optional components; ii) the inherent trade-off between antibacterial and anticorrosion bifunctionality; and iii) the ambiguous mechanism of achieving ideal formulations. Herein, we developed a data-driven framework that combines bi-objective global optimization with interpretable molecular interaction modules. This approach efficiently explored the vast formulation-performance landscape without pre-set optimization weights, enabling the discovery of the entire Pareto-optimal solutions of amino acid-surfactant pairs. Among these, an optimal formulation with balanced antibacterial and anticorrosion behavior showed the best performance in mitigating the microbial corrosion. Meanwhile, the crucial molecular interaction factors that determined the antibacterial and anticorrosion synergy of inhibitor pairs were found among the various possibilities. Notably, we demonstrated that relatively weak molecular interactions between amino acids and surfactants were paradoxically more favorable for the formation of ideal adsorption configurations on the metal surface. This work delivers a promising corrosion inhibitor discovery paradigm for addressing microbial corrosion, while offering a versatile closed-loop route for the rational design of multifunctional drug combinations.
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ACS Nano
·
2023
·
IF 17.1
Rui Gao,Changlong Hao,Liguang Xu,Xinxin Xu,Jing Zhao,Maozhong Sun,Qing Wang,Hua Kuang,Chuanlai Xu
Titanium dioxide (TiO2) has attracted significant attention in the fields of antibacterial activity and pollutant degradation due to its well-known photocatalytic properties. However, the application of TiO2 is significantly limited by its large band gap width, which only allows excitation by ultraviolet light below 400 nm. Here, we propose the use of surface chiral functionalization of TiO2 to tune its band gap width, thus enabling it to be excited by near-infrared-region light (NIR), resulting in the effective separation of electron–hole pairs. By controlling the solvent polarity and forming numerous weak interactions (such as hydrogen bonding) between chiral ligands and TiO2, we successfully prepared chiral TiO2 superparticles (SPs) that exhibited a broad circular dichroism (CD) absorption at 792 nm. Under circularly polarized light (CPL) at 808 nm, the chiral SPs induced the separation of electron–hole pairs in TiO2, thus generating hydroxyl and singlet oxygen radicals. Antibacterial tests under CPL in NIR showed that the chiral TiO2 SPs exhibited excellent antibacterial performance, with inhibition rates of 99.4% and 100% against Gram-positive and Gram-negative bacteria, respectively. Recycling–reuse experiments and biocompatibility evaluation of the material demonstrated that the chiral TiO2 SPs are stable and safe antibacterial materials, thus indicating the potential application of chiral TiO2 SPs in antibacterial aspects of medical implants.
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