INSTRUMENTATION SCIENCE & TECHNOLOGY
·
2020
·
IF 1.418
Li-Ye Niu,Qi Wang,Wan-Ming Zhao,Jian-Ying Jing
A D-type optical fiber surface plasmon resonance (SPR) sensor based on gold film and Au nanoshells is described. The sensor has a smaller full width at half maximum (FWHM) and a higher quality factor. The influence of the coupling effect between the Au nanoshells and gold film with different internal and external diameter ratios on the sensor was investigated by the finite element method. The simulation results show that the coupling effect between the SPR of the gold film and the localized surface plasmon resonance (LSPR) of the Au nanoshells effectively improved the electric field strength of the sensing region. The refractive index measurements show that the sensitivity of the D-type fiber/gold film/Au nanoshell (50/40 AuNS) SPR sensor is 3064 nm/RIU, which is 993 nm/RIU higher than for the D-type fiber/gold film SPR sensor. The D-type fiber/gold film/Au nanoshell (50/40 AuNS) SPR sensor also provides good linearity with a value of 99.4%. The sensor reported in this paper can be used for reference in the development of high performance biosensors.
研究领域:
传感器技术
·
光学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1181
·
IF 4.2
Minghe Wang,Zhouyuji Liao,Dongting Zhangsun,Yong Wu,Sulan Luo
Antimicrobial peptides (AMPs), acknowledged as host defense peptides, constitute a category of predominant cationic peptides prevalent in diverse life forms. This study explored the antibacterial activity of α-conotoxin RgIA, and to enhance its stability and efficacy, D-amino acid substitution was employed, resulting in the synthesis of nine RgIA mutant analogs. Results revealed that several modified RgIA mutants displayed inhibitory efficacy against various pathogenic bacteria and fungi, includingCandida tropicalisandEscherichia coli. Mechanistic investigations elucidated that these polypeptides achieved antibacterial effects through the disruption of bacterial cell membranes. The study further assessed the designed peptides’ hemolytic activity, cytotoxicity, and safety. Mutants with antibacterial activity exhibited lower hemolytic activity and cytotoxicity, with Pep 8 demonstrating favorable safety in mice. RgIA mutants incorporating D-amino acids exhibited notable stability and adaptability, sustaining antibacterial properties across diverse environmental conditions. This research underscores the potential of the peptide to advance innovative oral antibiotics, offering a novel approach to address bacterial infections.
研究领域:
药理学
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Hui Liu,Huiting Xu,Zhaofeng Song,Zhengwen Li,Saiwei Zhang,Yili Zhang,Jixiang Wang,Xiaoyong Qian
The types and concentrations of L- and D-type amino acids (AAs) produced by sludge hydrolysis increased with increasing pH of alkali treatment, with the highest content of cysteine and a higher degree of racemization of threonine, cysteine, and alanine. There was a strong correlation between methane production and the following AAs (D-leucine, D-aspartic acid, L-threonine, and L-cysteine). The addition of representative AAs (L-cysteine and D-cysteine) increased cumulative methane production, and L-type promoted better than D-type, with the highest cumulative methane production 906.3 mL/g-VS with the addition of 50 mg/L of L-cysteine, which was 1.20 times that of the CK group (digestion time 21 d); while the methane production with the addition of 200 mg/L of L-cysteine would be inhibited. The L-type AAs promoted better than the D-type in the solubilization, hydrolysis, acidification, and hydrogen-consumption methanogenesis stages, whereas the homoacetogenesis process and acetate-consumption methanogenesis stages did not play a significant role. The dominant genera of bacteria at the genus level belonged to unclassified_c_Acitinobacteria, unclassified_p_Chloroflexi, unclassified_c_Acidimicrobiia and Mycobacterium, while Methanothrix and Methanobacterium were the dominant archaea in the digesters. The top 4 metabolic functions were biosynthesis of secondary metabolites, microbial metabolism in diverse environments, biosynthesis of AAs and carbon metabolism. Metagenome results showed that, with L-cysteine addition, enriching Methanothrix and Methanobacterium showed enhanced contribution to the main pathways, especially the biosynthesis of secondary metabolites and carbon metabolism.
研究领域:
环境科学
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
IRANIAN POLYMER JOURNAL
·
2016
·Vol. 25
·期 5
·443-454
·
IF 1.684
Kong Lingqian,Ju Xiuping,Zhang Yan,Zhao Jinsheng,Xu Jianzhong
Abstract Two new donor–acceptor–donor (D–A–D) type monomers, 2-dodecyl-4,7-bis(4-methoxythiophen-2-yl)-2 H -benzo[ d ] [ 1 – 3 ] triazole (MOTBT) and 2-dodecyl-4,7-bis(4-methylthiophen-2-yl)-2 H -benzo[ d ] [ 1 – 3 ] triazole (MTBT) were synthesized by Stille coupling and the corresponding polymers, PMOTBT and PMTBT were obtained by electropolymerization method. The properties of the monomers and the polymers were studied by cyclic voltammetry (CV), ultraviolet–visible–near-infrared (UV–Vis–NIR) and spectroelectrochemistry. The CV results demonstrated ambipolar processes (n- and p-doping) for both polymers. According to the spectroelectrochemistry tests, the polymer PMOTBT revealed a saturated blue color in the reduced state and a transparent light blue color in the oxidized state, and the PMTBT polymer could switch between neutral orange color and oxidized light bluish-green color in the redox process. Furthermore, in the visible region, the optical contrasts were 51 % (at 645 nm) and 34 % (at 430 nm) for PMOTBT and PMTBT, respectively, with the switching times of 0.29 and 1.2 s from reduced state to oxidized state. In the NIR region, higher optical contrast ratios of 76 % (at 1600 nm for PMOTBT) and 67 % (at 1500 nm for PMTBT) as well as faster response times (0.90 and 0.35 s) were obtained. We also found that both polymers had the relative high coloration efficiency as much as 372 and 312 cm 2 /C and a satisfactory stability. Therefore, both polymers are promising candidates in displays and opto-electronic devices as electrochromic materials. Graphical abstract
研究领域:
材料科学
·
电化学
·
光学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Yanan Gao,Liwei Dou,Hong Gao,Jie Song,Shibin Shang,Zhanqian Song
D-π-D hole-transporting materials have the potential to be excellent dopant-free hole-transporting materials (HTMs) for perovskite solar cells (PSCs) because of their tunable energy levels, high charge mobility, good solubility, and excellent film-forming properties. In this paper, two series of D-π-D type compounds, Series 1 (2TPA-CZ1~2TPA-CZ3) and Series 2 (2DTPA-CZ1~2DTPA-CZ3), were synthesized by linking a carbazole derivative (as a π-bridge) to triaryamine or dehydroabietic acid triarylamine groups (the donor groups) via C–C coupling reactions. The optical, electrochemical, thermal, and photovoltaic properties in PSCs of these compounds were fully investigated. The two series of compounds exhibit good ultraviolet absorption, fluorescence emission , and electrochemical and thermal properties. In addition, their highest occupied molecular orbital levels and energy gaps make them suitable for use as hole-transporting materials in PSCs. Among the PSCs developed, Device 1, based on 2TPA-CZ1, showed the best performance, with a power conversion efficiency (PCE) of 12.27%, a open circuit voltage (V OC ) of 1.13 V, a short-circuit photocurrent density (J SC ) of 15.10 mA cm −2 , and a fill factor (FF) of 71.61%, which is comparable to that of a device based on the conventional hole-transporting material Spiro-OMeTAD. While the PCE of Devices 4–6 was lower than the PCE of Devices 1–3, Devices 4–6 exhibit better stability. This may be due to the higher T g and better film-forming properties of compounds formed using the dehydroabietic acid skeleton. In additional, the introduction of alkyl groups on the carbazole moiety decreased the efficiency of the PSC devices. These results demonstrate that the 2TPA-CZ1 compounds are promising HTMs for PSC applications.
研究领域:
材料科学
·
能源科学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Xi-Ling Li,Yu Han,Yuan Huang,Xiaoqi Sun,Shuyun Xiao,Jun Zhe Min
We developed a novel fluorescent chiral probe, DBD-trans-2-methyl-L-proline (DBD-M-Pro), which can be used to target recognition of amino functional groups using chiral resolution. To investigate the chiral resolution efficiency, 20 chiral amino enantiomers (19 DL-amino acids and phenylethylamine) were labeled using ultra-performance liquid chromatography (UPLC) with a fluorescence (FL) system. Diastereomers were formed by the reactions of DBD-M-Pro with enantiomers of amino functional groups at 60 °C for 60 min and detected on a BEH C18 column (100 × 2.1 mm, 1.7 μm). Gradient elution of 10 mM ammonium acetate with 0.05% formic acid (FA) aqueous solution and 0.1% FA acetonitrile or 0.1% FA methanol solution was performed at an excitation wavelength (Ex) 460 nm and emission wavelength (Em) 550 nm. Each resulting derivative of D- and L- type was effectively separated. The results showed that the resolution (Rs) of 17 amino acids and phenylethylamine (PEA) in the range of 1.59–24.11, except for histidine (His) (Rs = 1.32) and serine (Ser) (Rs = 1.47), achieved completely separation. The DBD-M-Pro chiral probe has a robust chiral selectivity for D-amino acids. Furthermore, a new method for the simultaneous determination of six DL-amino acids (Pro, Val, Trp, Phe, Leu, Lys) in human saliva was developed. The proposed method showed resolution values of 1.78–16.38, and an excellent linear relationship was obtained in the range of 2.5–500 pmol ( R 2 ≥ 0.9990). The limit of detection (S/ N = 3) ranged from 0.5 to 3.75 pmol. The intra-day and inter-day coefficient of variation (CV) were within the range of 1.75–11.73%. The average addition recoveries in saliva ranged from 95.99 to 106.97%. The methodology was used to determine the content of DL-amino acids and the D/L-amino acid ratio in the saliva of 40 healthy volunteers (15 males and 25 females), as well as evaluating the differences between men and women. Our study suggests that the DBD-M-Pro chiral probe could be an effective tool for screening potential D-amino acid biomarkers in disease.
研究领域:
分析化学
·
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Xing-Yan Fu,Hao Yin,Xi-Tong Chen,Jing-Fang Yao,Yan-Nan Ma,Min Song,Huan Xu,Qian-Yao Yu,Shan-Shan Du,Yun-Kun Qi,Ke-Wei Wang
Oncolytic peptides represent promising novel candidates for anticancer treatments. In our efforts to develop oncolytic peptides possessing both high protease stability and durable anticancer efficiency, three rounds of optimization were conducted on the first-in-class oncolytic peptide LTX-315. The robust synthetic method, in vitro and in vivo anticancer activity, and anticancer mechanism were investigated. The D-type peptides represented by FXY-12 possessed significantly improved proteolytic stability and sustained anticancer efficiency. Strikingly, the novel hybrid peptide FXY-30, containing one FXY-12 and two camptothecin moieties, exhibited the most potent in vitro and in vivo anticancer activities. The mechanism explorations indicated that FXY-30 exhibited rapid membranolytic effects and induced severe DNA double-strand breaks to trigger cell apoptosis. Collectively, this study not only established robust strategies to improve the stability and anticancer potential of oncolytic peptides but also provided valuable references for the future development of D-type peptides-based hybrid anticancer chemotherapeutics.
研究领域:
癌症研究
·
药物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Plasmonics
·
2025
·
IF 4.3
Zhang Yirui,Wang Zhiyang,Zhang Ailing,Chang Pengxiang,Li Zhen,Shi Yanmei
In this paper, a fiber-optic biosensor with the structure of both Au film and Au nanoparticles (AuNps) co-modified on D-shaped photonic crystal fiber (PCF) by adhesion with polydopamine (PDA) is proposed and experimentally fabricated, where the electric field near the sensing surface is increased and thus the sensitivity is improved by coupling the surface plasmon resonance (SPR) in Au film with the localized surface plasmon resonance (LSPR) in Au nanoparticles (AuNps), D-shaped PCF as well as high refractive index of PDA. In addition, due to excellent adhesion and biocompatibility properties of PDA, an extra layer of PDA is modified on the surface of AuNps to anchor a layer of goat anti-rabbit IgG on it, which is a bioassay for rabbit IgG. The experimental results show that the sensor obtains a high sensitivity of 4301.49 nm/RIU in the refractive index (RI) range of 1.335 ~ 1.365, which is 2.4 times the RI sensitivity of the PCF-Au sensor. The bio-sensitivity of the sensor for rabbit IgG reaches 1.017 nm/(µg/ml), and the limit of detection (LOD) is 0.019 µg/ml. This sensitized fiber-optic biosensor based on the SPR-LSPR coupling effect has a potential application in clinical disease diagnosis and immunoassay.
研究领域:
光学生物传感
·
纳米技术
·
生物光子学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhan Huang,Xing Huang,Yanfei Zhang,Meng Chen,Shaoqiang Wen,Zhou Zhang,Zong Dai,Xiaoyong Zou,Si-Yang Liu
DNAzyme-based gene silencing is a promising cancer therapy technology, while its efficiency and safety issues still need to be addressed. In this study, we developed a chirality/microRNA dual-gating theranostic nanomachine for messenger RNA (mRNA) gene silencing therapy with enhanced targeting towards human cervical cancer (HeLa) cells. The chiral nanoparticle, an emerging material with unique chirality-induced properties, e.g. chirality-dependent cellular uptake, has been widely applied in biomedical fields. The L-type gold nanoparticles (L-AuNPs) are used as the nanocarrier with the first “chirality-gating”. L-AuNPs showed 2.4-fold uptake efficiency for HeLa cells comparing to normal cell, as well as 2.2-fold uptake efficiency in cancer cell comparing to D-type gold nanoparticles (D-AuNPs), which exhibited no cancer cell targeting effect. Moreover, an entropy-driven-DNAzyme (EDz) circuit that can release numerous DNAzyme and fluorescence probes with the trigger of single microRNA (miRNA) conducted the theranostic role of both miRNA imaging and gene silencing, constituting the second “miRNA gating”. Ascribing to the prominent targeting nanocarrier L-AuNPs, the miRNA imaging sensitivity and gene silencing therapy efficiency were improved by 2.6- and 5.0-fold, respectively, comparing to those by D-AuNPs. In general, this dual-gating nanomachine, designed with a powerful yet simple strategy, has showed great prospects in optimizing delivery specificity and avoiding off-target toxicities.
研究领域:
癌症治疗
·
纳米技术
·
分子生物学
·
生物医学工程
·
基因治疗
✓ 已验证
产品关联
DOI
高影响因子
AMINO ACIDS
·
2021
·Vol. 53
·期 2
·195-204
·
IF 3.52
Yang Jiangxia,Li Xiaoqi,Du Yingxiang,Ma Mingxuan,Zhang Liu,Zhang Jian,Li Peipei
In this work, we prepared gold nanoparticles (AuNPs) by employing gluconic acid (GlcA) as reducing-cum-stabilizing agent. The proposed GlcA-AuNPs successfully worked as a colorimetric sensor for visual chiral recognition of aromatic amino acid enantiomers, namely tyrosine ( d/l -Tyr), phenylalanine ( d/l -Phe), and tryptophan ( d/l -Trp). After adding L-types to GlcA-AuNPs solution, the color of the mixture changed from red to purple (or gray), while no obvious color change occurred on the addition of D-types. The effect can be detected by naked eyes. The particles have been characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, zeta potential, the dynamic light scattering analysis as well as UV–Vis spectroscopy. This assay can be used to determine the enantiomeric excess of l -Trp in the range from 0 to + 100%. The method has advantages in simplicity, sensitivity, fast response, and low cost.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）