CHEMICAL ENGINEERING JOURNAL
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2026
·
IF 12.5
Yixuan Jiang,Yanlin Zhao,Fan Zhao,Xiaowei Cao,Dingding Jing,Haifeng Huang,Qinglei Hang
Chiral D Au NCs capillary SERS for label-free non-invasive saliva D-Glc assay. • Sensor achieves 0.30 nM LOD, covering clinical saliva glucose ranges. • Assay finishes in 10 min with 50 μL sample, high accuracy and anti-interference. • Assay shows correlation with GOD-POD gold std. (R 2 = 0.9313) and 0.947 AUC. • It offers a promising tool for early diabetes screening and clinical translation. Early, non-invasive screening and risk prediction of diabetes are crucial for effective prevention and control, as well as for delaying the onset and progression of complications. In this study, we developed a capillary surface-enhanced Raman scattering (SERS) sensor based on chiral gold nanocubes (D Au NCs) for the non-invasive, label-free, and rapid detection of d -glucose (D-Glc) in saliva. By leveraging the chiral recognition capability and strong SERS activity of D Au NCs, the sensor specifically identifies D-Glc and significantly amplifies its characteristic Raman signals, enabling quantitative analysis in complex saliva matrices without labeling. The sensor demonstrates outstanding analytical performance, covering the clinically relevant range of salivary glucose concentrations with a detection limit as low as 0.30 nM. Based on a capillary detection platform, the assay is simple and rapid, completed within 10 min using only a 50 μL micro-sample, while demonstrating strong anti-interference capability and high detection accuracy. Clinical saliva testing showed strong correlation with the glucose oxidase-peroxidase (GOD-POD) gold standard (R 2 = 0.9313), enabling reliable discrimination between healthy individuals and diabetic patients. Receiver operating characteristic (ROC) analysis yielded an area under the curve (AUC) of 0.947, confirming its high efficacy in diabetes risk screening and strong diagnostic performance. Consequently, the D Au NCs-based capillary SERS sensor provides a promising strategy for early, non-invasive diabetes screening and prevention, possessing broad potential for clinical translation and application.
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ANALYTICAL CHEMISTRY
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2026
·
IF 7.3
Simin Zhang,Xiangfeng Chen,Yaping Shi,Xiangyu Zhu,Zongwei Cai
As bioactive compounds, chiral amino acids (AAs) have been demonstrated to play pivotal roles in different physiological processes, thereby influencing human health. The assessment of the relative enantiomeric ratio through analytical methods is essential for indicating the abnormal status of disease progression, which drives the development of effective methods for resolving and identifying enantiomers. This work presents a robust strategy for the ultrahigh-resolution analysis of chiral AAs using MALDI-TIMS-MS. By employing a chiral derivatization process using S-NIFE and stabilizing the soft chain of the derivatization reagent with bimetal ion addition, the separation of 19 pairs of proteinogenic AAs was successfully achieved. This method not only demonstrates outstanding separation capability but also proves its efficiency in determining enantiomeric ratios, extending its applicability to real sample analysis.
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Journal of Materials Chemistry C
·
2025
·
IF 5.1
Ji Qi,Pibin Bing,Lanju Liang,Xin Yan,Haiyun Yao,Zhenhua Li,Ziqun Wang,Zhongyang Li,Qing Liu,Guifang Wu
Trace biological solution detection is currently of great interest in the terahertz (THz) field. Graphene oxide (GO) is an amphiphilic graphene-like material with favorable biocompatibility, showing good potential applicability in THz liquid detection. In this work, a GO enhanced THz metamaterial liquid biosensor with a polyimide capillary as the microfluidic channel is proposed. The experimental results reveal that the capillary structure can avoid the large absorption of THz waves by controlling the amount of water. In the biosensor, GO is demonstrated to play an important role in enhancing the THz response, improving surface adsorption, and facilitating signal transmission. It is able to enhance the immobilization of D-Asp, which was confirmed by both Raman spectra and XRD scans, thus resulting in the structural changes of GO. The transmission amplitude variation of THz waves indicates that the electromagnetic response of GO varies with the adsorbed D-Asp concentration. The proposed biosensor achieves an ultra-low detection limit of 3 pg mL−1 and a maximum transmission change of about 12.5%. Thus, with their robust resistance to water interference and outstanding detection performance, the proposed biosensors show potential advantages for the expanded application of THz metamaterials in the ultra-sensitive detection of trace biological samples in liquid environments.
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Solar RRL
·
2022
·Vol. 7
·期 4
·2200998
·
IF 9.173
Yonglei Han,Chao Zhou,Shuchen Weng,Guicheng Yu,Fang Lin,Hanlin Hu,Yongfei Wang,Haoran Lin
Perovskite solar cells (PSCs) are widely studied as the most promising photovoltaic technology, but their performance is sensitive to the morphology and the defect density of the perovskite films. Herein, additive engineering strategy is applied to further improve the film morphology and device performance by doping a small amount of natural product, D-Aspartic acid (D-2-Aminobutanedioic acid, D-Asp), into perovskite precursor solution. The modified device exhibits a greatly enhanced power conversion efficiency of 22.7%, which is unprecedented for PSCs doped with natural amino acids, and 3000 h stability in ambient air is achieved. Through systematical characterizations, it is concluded that D-Asp could result in thicker film formation, outstanding perovskite film morphology, and reduced defect sites passivated by the multiple functional groups. The results prove that the performance and stability of the state-of-the-art mixed ion PSCs with regular architecture could be effectively enhanced by D-Asp. The minimum usage of the natural product as an additive is beneficial for the fabrication process and cost-control in the industrialization of PSCs. This work also highlights the different passivating mechanisms for the molecules with multiple functional groups which are meaningful for materials design.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Wei Jia,Xin Wang
The potential mechanisms about the health risks of endogenous 3-MCPD remain elusive. Here, we researched the influences of 3-MCPD on the metabolic landscape of digested goat infant formulas via integrative UHPLC-Q-Orbitrap HRMS-MS/MS-based peptidomics and metabolomics (%RSDs ≤ 7.35 %, LOQ 2.99–58.77 μg kg −1 ). Digested goat infant formulas under 3-MCPD-interference caused metabolic perturbation by down-regulating levels of peptides VGINYWLAHK (5.98–0.72 mg kg −1 ) and HLMCLSWQ (3.25–0.72 mg kg −1 ) pertained to health-promoting bioactive components, and accelerated the down-regulation of non-essential amino acids (AAs, l -tyrosine 0.88–0.39 mg kg −1 , glutamic acid 8.83–0.88 μg kg −1 , and d -aspartic acid 2.93–0.43 μg kg −1 ), semi-essential AA ( l -arginine 13.06–8.12 μg kg −1 ) and essential AAs ( l -phenylalanine 0.49–0.05 mg kg −1 ) that provide nutritional value. Peptidomics and metabolomics interactions elucidated that 3-MCPD altered the stability of α-lactalbumin and d -aspartate oxidase in a dose-dependent manner, and affected the flavor perception of goat infant formulas, leading to a decline of nutritional value of goat infant formulas.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-14
·
IF 17.694
Arabi Maryam,Ostovan Abbas,Wang Yunqing,Mei Rongchao,Fu Longwen,Li Jinhua,Wang Xiaoyan,Chen Lingxin
Chiral discrimination is critical in environmental and life sciences. However, an ideal chiral discrimination strategy has not yet been developed because of the inevitable nonspecific binding entity of wrong enantiomers or insufficient intrinsic optical activities of chiral molecules. Here, we propose an “inspector” recognition mechanism (IRM), which is implemented on a chiral imprinted polydopamine (PDA) layer coated on surface-enhanced Raman scattering (SERS) tag layer. The IRM works based on the permeability change of the imprinted PDA after the chiral recognition and scrutiny of the permeability by an inspector molecule. Good enantiomer can specifically recognize and fully fill the chiral imprinted cavities, whereas the wrong cannot. Then a linear shape aminothiol molecule, as an inspector of the recognition status is introduced, which can only percolate through the vacant and nonspecifically occupied cavities, inducing the SERS signal to decrease. Accordingly, chirality information exclusively stems from good enantiomer specific binding, while nonspecific recognition of wrong enantiomer is curbed. The IRM benefits from sensitivity and versatility, enabling absolute discrimination of a wide variety of chiral molecules regardless of size, functional groups, polarities, optical activities, Raman scattering, and the number of chiral centers.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Haoyuan Li,Hongxiang Zhu,Zongyan Quan,Zhiping Chen,Lei Wang,Hui He
Gold nanoclusters with excellent fluorescence intensity, stability and biocompatibility have promising application prospects in the field of detection. Inspired by the growth behavior of sea squirts adhered to ropes, a novel bionic bifunctional cellulose nanocrystal (CNC)-based gold nanocluster probe was effectively constructed by directionally growing gold nanoclusters on CNCs modified with a specific DNA sequence . The constructed probe has excellent fluorescence properties and stability. The “ON or OFF” conversion mode of fluorescence was designed to detect different pathological markers in cells. In the “ON” mode, the probe readily combined with various sulfhydryl group containing markers (acetylcholinesterase, cysteine and glutathione) to produce fluorescence quenching and realize detection, the corresponding detection limits were as low as 0.015 mU/mL, 0.028 μM and 0.228 μM, respectively. Interestingly, the fluorescence of the probe could be regulated to the OFF mode via the assembly of the graphene oxide structure in accordance with Förster resonance energy transfer . The fluorescent probe with OFF mode could selectively recognize the carcinoembryonic antigen biomarker of malignant tumours via fluorescence enhancement, and its limit of detection was as low as 0.54 ng/mL. The as-developed novel strategy has positive significance for the early diagnosis of malignant tumours.
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TALANTA
·
2022
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IF 6.556
Shutong Yang,Liancheng Gu,Fangling Wu,Xinhua Dai,Fuxing Xu,Qiaoyu Li,Xiang Fang,Shaoning Yu,Chuan-Fan Ding
Chiral recognition is of highly interest in the areas of chemistry , pharmaceuticals, and bioscience. An effective strategy of enantiomeric determination of amino acids (AAs) was developed in this work. All 19 natural AAs enantiomers can be easily distinguished by ion mobility-mass spectrometry of the non-covalent complexes of AAs with cyclodextrins (α-CD, β-CD and γ-CD) and Mg 2+ without any chemical derivatization . Differences of the mobilities between the enantiomers’ complexes is from 0.006 to 0.058 V s/cm 2 . In addition, the complex of [β-CD + Phe + Mg] 2+ was selected as an example to study the relative quantification by measuring L/D-Phe at different molar ratio of 10:1 to 1:10 in the μM range, resulting in a good linearity (R 2 > 0.99) and high sensitivity at 2 μM. A DFT calculation was also performed to illustrate the detailed molecular structure of the complexes of CDs , Mg 2+ and D- or L-Phe. Both experiment and theoretical calculation showed that Mg 2+ plays an important role in host/guest interactions, which changed the molecular conformations by non-covalent interaction between Mg 2+ and CDs, and resulted in the different collision cross-sections of the complex ions of CDs, Mg 2+ and D- or L-AAs in the gas phase. This effective and convenient strategy could potentially be utilized in scientific research and industry for routine enantiomeric determination of natural AAs, peptides and some other small chiral biomolecules such as non-natural AAs and carboxylic acid-containing drugs.
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Materials Chemistry Frontiers
·
2022
·Vol. 6
·期 22
·3391-3401
·
IF 8.683
Shuli Liu,Xiaoling Wu,Jun Xiong,Xin Yuan,Min-Hua Zong,Wen-Yong Lou
Enzymatic cofactor regeneration is of significant importance and highly desired for tremendous biocatalytic processes, which is hindered by fragility intrinsic to natural enzymes. Herein, we report a copper aspartic acid (CuAsp) metal–organic framework (MOF) with a fiber structure exhibiting both NADH peroxidase- and glycerol dehydrogenase (GlyDH)-mimicking activities. The growth mechanism of CuAsp was investigated by changing the functional groups of ligands, which demonstrated that the amino group and two carboxylate groups in aspartic acid were all involved in the formation of MOFs. The CuAsp MOF displayed high NADH peroxidase mimicking activity within a wide range of pH and temperature. A cofactor total turnover number (TTN) of up to 17 500 was obtained in a typical model reaction of glucose dehydrogenase-catalyzed oxidation of glucose. More interestingly, CuAsp exhibited glycerol dehydrogenase (GlyDH)-mimicking activity and showed higher catalytic efficiency (with kcat/Km of 14.42 mL g−1 min−1) than its native counterpart (with kcat/Km of 2.40 mL g−1 min−1). As a proof of concept, CuAsp with the dual function of NADH peroxidase and GlyDH-mimicking activities was used to catalyze the transformation of glycerol to 1,3-dihydroxyacetone (DHA). Besides, free GlyDH was encapsulated into CuAsp via a facile self-assembly process, which enabled the synergistic biosynthesis of DHA. This study may provide new insights into amino acid-based MOFs and suggest the potential of multifunctional nanozymes in the application of biocatalysis and biosensing.
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Analytical Methods
·
2021
·Vol. 13
·期 16
·1905-1910
·
IF 2.896
Lamei Yang,Feng Luo,Weili Wei
The chirality of amino acids plays an important role in biological and medical sciences. The development of achiral small-molecule probes that can simultaneously determine the absolute configuration, enantiomeric excess, and total concentration of amino acids is significant. We reported the currently available achiral coumarin aldehyde probe that could form Schiff bases with free amino acids at room temperature to induce CD signals and change UV-vis signals. The red-shifted UV-vis signals were independent of the substrate's chirality and could be used to determine the total concentration. Conversely, the enantioselective CD signals could be used to determine the absolute configuration and enantiomeric excess. The usefulness and practicability of this sensing method were demonstrated by analyzing 6 non-racemic phenylalanine samples with different enantiomeric compositions and concentrations.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Wen Luo,Huaxiao Liu,Xuan Liu,Lixiao Liu,Wenbo Zhao
MXene quantum dots have attracted much attention due to their great optical performance and excellent water solubility. Glutathione (GSH) plays a key role in living cells. In this study, a biocompatibility nanoprobe was prepared for detecting intracellular GSH based on MXene N-Ti 3 C 2 quantum dots (N-Ti 3 C 2 QDs). The N-Ti 3 C 2 QDs act as the fluorescence reporters and the ferric iron (Fe 3+ ) as the quenchers based on nonradiative electron-hole annihilation. When Fe 3+ encounters the amino group of N-Ti 3 C 2 QDs, the electrons of N-Ti 3 C 2 QDs in the excited state will transfer to the half-filled 3d orbitals of Fe 3+ , leading to the fluorescence quenching of N-Ti 3 C 2 QDs. When the N-Ti 3 C 2 QDs/Fe 3+ nanoprobe acts on the cancer cell MCF-7, the abundant GSH in the cancer cells can reduce Fe 3+ to Fe 2+ , which will restore the fluorescence of N-Ti 3 C 2 QDs. The N-Ti 3 C 2 QDs/Fe 3+ nanoprobe displays a high sensitivity for GSH with a detection limit of 0.17 μM in range of 0.5−100 μM. It becomes a promising probe for detecting and showing cellular imaging of GSH in MCF-7 cells. The N-Ti 3 C 2 QDs/Fe 3+ nanoprobe might provide a new way for imaging-guided precision cancer diagnosis.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Yang Dan,Mei Shiliang,Wen Zhuoqi,Wei Xian,Cui Zhongjie,Yang Bobo,Wei Chang,Qiu Yi,Li Min,Li Hui,Zhang Wanlu,Xie Fengxian,Wang Le,Guo Ruiqian
Dipicolinic acid (DPA) is employed as a significant biomarker to detect Bacillus anthracis , which can do serious damages to the health of human beings. Hence, it is crucial to develop a fast and highly efficient strategy for DPA monitoring. In this work, based on silicon nanoparticles (Si NPs) and terbium metal-organic frameworks (Tb-MOFs), a hybrid structure (Si NPs/Tb-MOFs) as a novel dual-emitting fluorescence probe was fabricated for ratiometric detection of DPA, where blue light-emitting Si NPs (Ex: 280 nm; Em: 422 nm) are encapsulated into green light-emitting Tb-MOFs (Ex: 280 nm; Em: 547 nm). The optical properties and chemical composition of the as-obtained Si NPs/Tb-MOFs were characterized in detail. The Si NPs/Tb-MOFs probe not merely possesses the merits of a facile synthesis method but also is an excellent fluorescence probe. The response time towards DPA is less than 30 s, revealing that the process of detecting DPA can be completed in such a short time. The limit of detection for DPA is 5.3 nM, which is four orders of magnitude lower than an infectious dosage of anthrax spores for human beings (60 μM). This dual-emitting Si NPs/Tb-MOFs probe with interference-free and self-calibrating properties may be a potential candidate for further development in medical diagnosis.
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