GREEN CHEMISTRY
·
2020
·Vol. 23
·期 3
·1382-1388
·
IF 9.48
Liang Chen,Yiming Huang,Ren Zou,Jiliang Ma,Yunyi Yang,Tingzhen Li,Mingsai Li,Qi Hao,Haibo Xie,Xinwen Peng
Synthesis of D-xylonic acid, a high value chemical feedstock, from biomass chemicals through a photocatalytic process is of great significance in respect of economical and sustainable concerns, and therefore has aroused considerable attention. Herein, a TiO2/Ti3C2(TT) photocatalyst was designed for the oxidation of xylose to D-xylonic acid under ambient conditions. The photocatalyst is fabricated by in situ growth of TiO2 nano-particles on Ti3C2 nanosheets and exhibits 64.2% of D-xylonic acid yield with excellent stability. The mechanistic study indicates that the electron transfer from TiO2 to Ti3C2 significantly increases the life time of light-induced charge carriers, and hence improved photocatalytic performance.
研究领域:
材料科学
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化学工程
·
可持续化学
·
光催化
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产品关联
DOI
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2018
·
IF 4.521
Xiaoqian Guo,Feng Guo,Yishan Li,Zhangqin Zheng,Zhexu Xing,Zihan Zhu,Ting Liu,Xin Zhang,Ying Jin
Dehydration of D-xylose to yield furfural was carried out using bimetallic salts of a heteropolyacid as the catalyst at 160–220 °C in the DMSO/H 2 O mixtures. The effect of Sn/Cs molar ratio of the bimetallic salts of 12-tungstophosphoric acid (PW) obtained by ultrasound-assisted coprecipitation on dehydration of D-xylose was investigated. The resultant catalysts were characterized by X-ray diffraction, N 2 adsorption, field emission scanning electron microscopy and energy dispersive X-ray (EDX). It was found that the Sn-Cs codoped PW catalysts retained the Keggin crystal structure of PW. Sn 0.625 Cs 0.5 PW was the most active catalyst in the dehydration of D-xylose into furfural. The maximum D-xylose conversion (close to 100 wt %) and furfural yield (63 wt %) were achieved at 200 °C for 3 h in DMSO/H 2 O mixtures. It was found that 16.7 wt % Sn 0.625 Cs 0.5 PW on a chitosan-derived support displayed similar catalytic activity to that of Sn 0.625 Cs 0.5 PW and good stability after recycling six times.
研究领域:
化学工程
·
催化材料
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产品关联
DOI
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 66
·34944-34949
·
IF 3.708
Jintao Lin,Shuangyue Li,Mingzhong Sun,Cuili Zhang,Wenbo Yang,Zehui Zhang,Xiaojie Li,Shao Li
The economical production of biodiesel from lignocellulose hydrolysates requires a robust D-xylose fermentation path. The present work found that Lipomyces starkeyi AS 2.1560 and Rhodosporidium toruloides AS 2.1389 could produce micro-lipid effectively from D-xylose solution by a two-stage culture mode. Results demonstrated that both L. starkeyi and R. toruloides could achieve high lipid production in xylose solution. High cell density fermentation was carried out by a feed-batch fermentation mode. After fermentation for 48 h in a bioreactor using unsterile xylose solution by L. starkeyi, the biomass, lipid and lipid content reached 97.4 g L−1, 63.9 g L−1, and 65.5%, respectively. Accordingly, the lipid productivity reached 1.3 g L−1 h−1 in the second stage. And the overall lipid productivity was 0.55 g L−1 h−1. Therefore, L. starkeyi and R. toruloides are highly effective D-xylose-fermenting oleaginous yeasts for lipid production in xylose solution. The two-stage culture method should provide a new opportunity to make the industrial production of biodiesel possible in the future.
研究领域:
微生物学
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生物技术
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可再生能源
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产品关联
DOI
高影响因子
CATALYSIS COMMUNICATIONS
·
2023
·
IF 3.7
Linsong Yang,Yuting Liu,Yu Peng,Yu-Cai He,Cuiluan Ma
The feasibility of chemobiocatalytic production of furfuryl alcohol from biomass-derived D -xylose was investigated using a tandem catalysis with deep eutectic solvent betaine:benzenesulfonic acid (DES BE:BA) and recombinant Escherichia coli DCF cell. The DES BE:BA (2.5 wt%) was applied to catalyze D -xylose under 170 °C for 0.5 h, resulting in a furfural yield of 51.1%. Consequently, recombinant Escherichia coli DCF fully transformed D -xylose-derived furfural to furfuryl alcohol in DES BE:BA-water under pH 7.5 and 40 °C using HCOONa as cosubstrate. This eco-friendly chemobiocatalytic strategy was successfully applied for transforming D -xylose to furfuryl alcohol in DES BE:BA-water.
研究领域:
生物化学
·
可持续化学
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2022
·
IF 5.089
Hengtao Tang,Zheng Zhou,Zhi Chen,Xin Ju,Liangzhi Li
Monosaccharides from biomass can be used as cost-effective raw materials to produce rare sugars which are valuable sweeteners. Enzymes play a pivotal role in such biomass biorefinery processes to develop novel synthetic routes, and improve the environmental friendliness of current routes. Here we propose a proof of concept biorefinery process to prepare useful rare sugars of D-xylulose and D-ribulose from D-xylose which was abundant in biomass. Ribose-5-phosphate isomerase B from Ochrobactrum sp. (OsRpiB) with a unique substrate scope was engineered to intensify the activity of transforming D-xylose to D-xylulose. A double mutant R94N/N137Q with 80% higher catalytic efficiency was identified, and kinetic and molecular dynamic studies were conducted to explore the mechanism underneath. The results revealed a correlation between enhancing stability and activity simultaneously. Then a one-pot enzyme cascade was constructed with OsRpiB and D-tagatose-3-epimerase from Pseudomonas cichorii and transformed D-xylulose further to D-ribulose. The conversions of D-xylose and D-xylulose in cascade reaction were 1.80-fold and 1.83-fold of that in separate reactions with the aid of process parameter adjustment. The presented work is the first reported enzyme cascade converting D-xylose to D-ribulose, and proves the promising role of monosaccharide isomerase platforms in the refinery of biomass.
研究领域:
生物化学
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酶工程
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生物质精炼
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhe Dou,Pei-Ru Hu,Lin-Lin Song,Yi-Cheng Wang,Hao-Nan Tang,Zhen-Qi Weng,Ya-Jun Wang,Yuan-Qiang Cai
Carbonyl reductases (CRs) are promising biological macromolecules for the asymmetric synthesis of chiral secondary alcohols. A novel CR was evolved for efficient conversion of d -xylose into anti-aging ( S )-Pro-Xylane. Through extensive gene mining, an active carbonyl reductase Zm CR was identified from Zygofabospora marxiana with high reductive activity towards d -xylose-derived 1-C-(β- d -xylopyranosyl)-acetone (XPSA) and in situ cofactor regeneration capability. Using a three-round of concise engineering strategy, a hextuple mutant C85V/V128C/S129A/V161S/Y166H/T217A (M6) was identified from merely 157 mutants with specific activity of 46.00 U·mg −1 , about 306 folds of WT. The Shannon-Wiener index of M6 was 2.21, much higher than WT, indicating its extended substrate scope. M6 could efficiently catalyze the asymmetric reduction of 190 g·L −1 XPSA into ( S )-XPSP. The k cat / K M XPSA of M6 was 203.84 s −1 ·mM −1 , about 910 folds of WT. More non-bonded interactions were monitored in M6, contributing to the stabilization of reactive catalytic conformation. This study provides a promising biocatalyst for the chemoenzymatic synthesis of value-added anti-aging ( S )-Pro-Xylane from lignocellulosic d -xylose.
研究领域:
生物化学
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酶工程
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合成生物学
✓ 已验证
DOI
PubMed
高影响因子
Metabolic Engineering Communications
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2018
·
IF 0
Xiaojing Jia,Robert M. Kelly,Yejun Han
( R )-acetoin is a four-carbon platform compound used as the precursor for synthesizing novel optically active materials. Ethylene glycol (EG) is a large-volume two-carbon commodity chemical used as the anti-freezing agent and building-block molecule for various polymers. Currently established microbial fermentation processes for converting monosaccharides to either ( R )-acetoin or EG are plagued by the formation of undesirable by-products. We show here that a cell-free bioreaction scheme can generate enantiomerically pure acetoin and EG as co-products from biomass-derived D -xylose. The seven-step, ATP-free system included in situ cofactor regeneration and recruited enzymes from Escherichia coli W3110, Bacillus subtilis shaijiu 32 and Caulobacter crescentus CB 2. Optimized in vitro biocatalytic conditions generated 3.2 mM ( R )-acetoin with stereoisomeric purity of 99.5% from 10 mM D -xylose at 30 °C and pH 7.5 after 24 h, with an initial ( R )-acetoin productivity of 1.0 mM/h. Concomitantly, EG was produced at 5.5 mM, with an initial productivity of 1.7 mM/h. This in vitro biocatalytic platform illustrates the potential for production of multiple value-added biomolecules from biomass-based sugars with no ATP requirement.
研究领域:
生物化学
·
合成生物学
·
代谢工程
✓ 已验证
DOI
PubMed
CATALYSIS TODAY
·
2014
·
IF 3.309
Huiling Li,Aojie Deng,Junli Ren,Changyu Liu,Wenju Wang,Feng Peng,Runcang Sun
One of the most promising strategies for furfural production is to extract continually the target product from the aqueous solution utilizing organic solvents. With the aim to develop an ecologically viable catalytic pathway for furfural production without the addition of mineral acids, we presented a modified biphasic system using a solid acid (SO 4 2− /TiO 2 -ZrO 2 /La 3+ ) as catalyst for producing furfural from xylose. Different kinds of aprotic organic solvents (DMSO, DMF and DMI) in water phase and 2-butanol in organic phase (MIBK) were investigated as reaction media. Furfural yield and xylose conversion efficiency were dependent on the amounts of aprotic organic solvents and 2-butanol, the solid/liquid ratio, and the volume ratio of the organic phase and the aqueous phase as well as the reaction temperature and time. As a result, DMI showed the best performance on improving furfural yield during the furfural production. 3563.3 μmol of furfural/g of xylose with 97.9% xylose conversion efficiency was obtained after 12 h at 180 °C when the volume ratios of water to DMI and MIBK to 2-butanol were 8:2 and 7:3, respectively.
研究领域:
生物化学
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
npj Biofilms and Microbiomes
·
2023
·Vol. 9
·期 1
·1-14
·
IF 9.2
Hu Jie,Wu Yifan,Kang Luyuan,Liu Yisi,Ye Hao,Wang Ran,Zhao Jinbiao,Zhang Guolong,Li Xilong,Wang Junjun,Han Dandan
Elimination of specific enteropathogenic microorganisms is critical to gut health. However, the complexity of the gut community makes it challenging to target specific bacterial organisms. Accumulating evidence suggests that various foods can change the abundance of intestinal bacteria by modulating prophage induction. By using pathogenic Escherichia coli ( E. coli ) ATCC 25922 as a model in this research, we explored the potential of dietary modulation of prophage induction and subsequent bacterial survival. Among a panel of sugars tested in vitro, D -xylose was shown to efficiently induce prophages in E. coli ATCC 25922, which depends, in part, upon the production of D -lactic acid. In an enteric mouse model, prophage induction was found to be further enhanced in response to propionic acid. Dietary D -xylose increased the proportion of Clostridia which converted D -lactic acid to propionic acid. Intestinal propionic acid levels were diminished, following either oral gavage with the dehydrogenase gene ( ldhA )-deficient E. coli ATCC 25922 or depletion of intestinal Clostridia by administration of streptomycin. D -Xylose metabolism and exposure to propionic acid triggered E. coli ATCC 25922 SOS response that promoted prophage induction. E. coli ATCC 25922 mutant of RecA, a key component of SOS system, exhibited decreased phage production. These findings suggest the potential of using dietary components that can induce prophages as antimicrobial alternatives for disease control and prevention by targeted elimination of harmful gut bacteria.
研究领域:
微生物学
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胃肠病学
·
营养学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 10
·2369-2376
·
IF 3.864
Fan Hu,PinPing Wu,RuYa Wang,Wei Liu,Hua He
Herein, a simple, novel, and rapid colorimetric sensor for cimetidine (Cim) detection based on D-xylose protected gold nanoparticles (D-x@AuNPs) has been developed for the first time. The D-x@AuNPs were characterized by UV-vis, TEM and FT-IR techniques. Cimetidine causes the aggregation of D-x@AuNPs due to the formation of a strong covalent Au–N bond and electrostatic binding. As the cimetidine concentration increased, the color of the solutions gradually changed from wine-red to blue, and the large absorption band shifted from 533 to 680 nm upon D-x@AuNP aggregation. The effects of different experimental parameters were investigated. A comparative study on the detection of Cim using citrated capped AuNPs and D-x@AuNPs was presented. Under optimum conditions, the UV-vis spectra showed that the absorption ratio (A680/A533) increased linearly with the concentration of cimetidine in the range of 7 × 10−8 to 3 × 10−6 M with a correlation coefficient of 0.9956 and a limit of detection of 1 × 10−8 M without needing any complicated instruments. The selectivity of the D-x@AuNP detection system for cimetidine was excellent when compared with other ions and analytes. Due to their rapid and visible color changes, and their remarkable selectivity, the D-x@AuNPs synthesized in this study were suitable and could be applied to the detection of cimetidine in human urine.
研究领域:
分析化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 86
·70139-70146
·
IF 3.84
Xing-Long Li,Tao Pan,Jin Deng,Yao Fu,Hua-Jian Xu
Furfural is a biomass-based bulk chemical and its derivatives have potential applications as renewable fuels and chemicals. A water-tolerant and stable solid acid catalyst modified hydrated tantalum oxide (TA-p) was developed for catalytic conversion of D-xylose to furfural in water–organic solvent biphasic system. This process was performed both in a batch reactor and a continuous fixed-bed reactor. In the batch process, D-xylose conversion and furfural yield were significantly affected by the organic solvent, reaction temperature and reaction time. 1-Butanol, which could be obtained through the fermentation of biomass-based carbohydrates, was selected as organic phase and the highest furfural yield of 59% was achieved with D-xylose conversion of 96% at 180 °C in the continuous process. Moreover, the long-time stability test for 80 h under the optimal conditions showed the excellent stability of TA-p catalyst.
研究领域:
生物化学
·
催化化学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2017
·
IF 1.174
Lei Guo,Yangdong Hu,Lianying Wu,Chen Liang,Weitao Zhang
To achieve green hydrolysis technology of hemicellulose through repeated using hydrolysate, the hydrolysis of hemicellulose in corncob was studied. The influence of repeated use of corncob hydrolysate on concentrations of d -xylose and l -arabinose was investigated. The loss rates of d -xylose in the prepared d -xylose solutions both with and without corncob, and repeated using corncob hydrolysate under identical acidity condition were discussed. The result shows that d -xylose concentration and l -arabinose concentration are all gradually increasing with the growing time of repeated use of corncob hydrolysate. After the fifth repetition, the concentrations of d -xylose and l -arabinose are 196.7 g·L − 1 and 22.0 g·L − 1 , respectively. Substance inhibiting the degradation of d -xylose is generated during repeated use of corncob hydrolysate, and the production is further proved by the change of d -xylose concentration and the loss rate of d -xylose over heating time.
研究领域:
生物技术
·
化学工程
·
可持续化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）