INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2017
·
IF 2.962
Ee Li,Jiajia Wu,Dun Zhang,Yan Sun,Juna Chen
D-phenylalanine (D-Phe) was tested for its effect on the corrosion of Q235 carbon steel caused by Desulfovibrio sp. which belongs to sulfate reducing bacteria (SRB). Weight loss results indicated that the corrosion rate of Q235 coupons exposed to D-Phe was reduced. Much less pits were found on the surface of these coupons. It was found that pH and dissolved oxygen content of bacteria cultures as well as the growth of bacteria were not affected by D-Phe. However, the cell shape of D . sp. was changed to a more filamentous or spherical shape by treatment with D-Phe, and the structure of biofilms formed by bacteria with transformed shapes was looser and thinner. Moreover, the hydrogen sulfide production of D . sp. was inhibited by D-Phe. These results suggest that D-Phe may inhibit the corrosion process through two ways, i, D-Phe alters the bacteria shape, which inhibits the formation of biofilm and changes the structure of it consequently; ii, D-Phe reduces the production of hydrogen sulfide .
研究领域:
微生物腐蚀
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材料科学
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环境微生物学
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金属腐蚀与防护
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生物膜研究
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高影响因子
CHIRALITY
·
2026
·Vol. 38
·期 8
·e70131
·
IF 2.4
Xiaohong Xue,Sheng Gong,Jiali Wen,Zhentong Guan,Sichang Peng,Zongxia Guo
The effective chiral resolution of amino acid racemates has important significance in fields such as medicine and pharmaceuticals. By using phenylalanine as additive, the chiral resolution of aspartic acid racemate was investigated through crystallization method. It reveals that the enantiomeric excess of the crystalline product is affected by the seeding temperature, crystallization temperature, crystallization time, seed dosage, phenylalanine dosage, and the relative supersaturation. Under optimized condition, the average enantiomeric excess could reach 85% after a single crystallization in water. The excess enantiomer of the crystalline product is determined by the configuration of phenylalanine. The enantiomeric excess and the excess enantiomer were confirmed by chiral high performance liquid chromatography. The powder X-ray diffraction and Fourier transform infrared spectral results show that conglomerate was formed in the crystalline product. Seed and additive both play great role in the chiral resolution. The findings could give inspirations for efficient resolution of aspartic acid racemate into enantiopure compounds.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Simin Zhang,Xiangfeng Chen,Yaping Shi,Xiangyu Zhu,Zongwei Cai
As bioactive compounds, chiral amino acids (AAs) have been demonstrated to play pivotal roles in different physiological processes, thereby influencing human health. The assessment of the relative enantiomeric ratio through analytical methods is essential for indicating the abnormal status of disease progression, which drives the development of effective methods for resolving and identifying enantiomers. This work presents a robust strategy for the ultrahigh-resolution analysis of chiral AAs using MALDI-TIMS-MS. By employing a chiral derivatization process using S-NIFE and stabilizing the soft chain of the derivatization reagent with bimetal ion addition, the separation of 19 pairs of proteinogenic AAs was successfully achieved. This method not only demonstrates outstanding separation capability but also proves its efficiency in determining enantiomeric ratios, extending its applicability to real sample analysis.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Sipeng Tian,Baobing Tang,Mingzi Sun,Zhangpeng Xu,Tongtong Zhai,Huanhuan Xing,Bolong Huang,Yunling Liu,Jing Li,Erkang Wang
Classic luminol-H2O2 chemiluminescence (CL) systems are plagued by inherent self-decomposition, which compromises detection reliability. Although luminol-dissolved oxygen systems circumvent this issue, their ultralow reactivity necessitates innovative catalytic microreactor design. Herein, we pioneer a hydrogen-bonded organic framework (HOF) constructed from metal–organic square units and 1,2-propanediamine ligands as terminal hydrogen-bonding sites, achieving an unprecedented 4284-fold CL enhancement in luminol-O2 systems. HOF with porous zeolite-like supramolecular assemblies (ZSA) serves as the coreaction accelerator with strong O2 adsorption at tetrahedral Co–N/O sites that activates O2 to generate reactive oxygen species (ROS). And the kinetic confinement effect provided by the ZSA host with suitable channel and hydrogen-bonding contributions efficiently captured the luminol emitter in the nanocages, shortening electron-transfer pathways and reducing nonradiative energy loss. And the synergistic confinement catalysis enhanced CL performance is linearly related to the O2 adsorption capacity of the ZSA series (ZSA-1, ZSA-3, and ZSA-4). Theoretical calculations further confirmed that the adsorption of O2 and luminol in ZSA-1 optimizes the electron-transfer pathways to promote CL emission performances compared with those of zeolitic imidazolate framework-67 (ZIF-67). This work not only expands the application of HOF in the field of CL but also provides new insights for engineering materials to boost the CL photon emission efficiency of weak luminol-O2 systems.
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Biosensors-Basel
·
2025
·Vol. 15
·期 10
·700
·
IF 5.6
Yu Qin,Jiali Xie,Shujun Zhen
Fluorescence anisotropy (FA) has been widely used for analyzing biomolecules due to its high throughput, homogeneous detection, and strong resistance to photobleaching. However, the traditional FA method suffers from low sensitivity when the target molecules are small and rotate rapidly, often producing insignificant changes in the FA value. In this study, by combining double signal amplification through the trans-cleavage of CRISPR-Cas12a and DNA tetrahedron assemblies with a large molecular size, a new, fast, simple and highly sensitive FA method was constructed to achieve the quantitative detection of hepatitis B virus DNA (HBV-DNA). The experimental results showed that the linear range of this method was 0.5–9 nmol/L, and the detection limit (LOD = 3σ/k) was 48 pmol/L. In addition, the method demonstrated excellent selectivity and anti-interference, and it was successfully applied to detect HBV-DNA in human serum, indicating that this method has the potential for clinical diagnosis.
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Food Bioscience
·
2026
·
IF 6.2
Mengmeng Ji,Jinwei Li,Liuping Fan,Liang Wang,Shengquan Huang
Pseudomonas aeruginosa , a significant opportunistic pathogen, poses a serious threat to food safety due to its antibiotic resistance and strong biofilm-forming ability. In this study, quercetagetin (Que) was identified as a novel quorum sensing (QS) inhibitor. Reporter strain assays, molecular docking, and dynamic simulation indicated that Que acts as an antagonist of the QS receptors. Que attenuated QS-regulated virulence phenotypes in a dose-dependent manner but showed insufficient antibiofilm activity ( p >0.05) at sub-minimum inhibitory concentration (sub-MIC) levels. In contrast, the combination of 0.5 mmol/L Que with 20 mmol/L D-leucine or D-tryptophan exhibited additive effects on planktonic cells, reducing its survival by 2.19–3.97 log compared to the control. For biofilm cells, the combined treatment potentiated the reduction in cell density by 0.48–1.42 log. Notably, these effects were strain-dependent but were equal to or outperformed those of the ε-polylysine (ε-PL) control. Mechanistically, this enhancement was driven by the disruption of biofilm matrix and cell membrane integrity. This was evidenced by the greater suppression of Acyl-homoserine lactones (AHLs) synthesis and virulence phenotypes, alongside substantial reductions in biofilm biomass and roughness and increased in membrane permeability compared to Que. Furthermore, the combination resulted in repression of key enzymes activities (Na + -K + -ATPase and succinate dehydrogenase) by 29%–38%. These changes indicated a promising strategy to control P. aeruginosa and enhance food safety.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9
Hanbo Shi,Nannan Zhang,Wei Guo,Tancheng Dong,Bihui Zhou,Lanlan Yu,Baojun Liu,Jincheng Mu
Birnessite-type δ-MnO 2 is a promising candidate for low-temperature NH 3 -SCR reaction owning to its excellent redox properties. However, its performance is severely hampered by the excessive oxidation state of Mn species, which induces strong NH 3 chemisorption and subsequent over-oxidation to NO x . Herein, phenylalanine-functionalized δ-MnO 2 nanosheets (MnO x -Phe) were developed, leveraging Phe's coordinating –NH 2 group to modulate surface properties of Mn species. The optimal MnO x -Phe-0.1 catalyst achieves ∼90% NO x conversion at 75 °C with a broad temperature window from 75 to 210 °C (GHSV = 50,000 h −1 ), representing a reaction rate 4.8 to 8.2 times greater than that of pure δ-MnO 2 . Additionally, the catalyst exhibits remarkable resistance against SO 2 and H 2 O. Comprehensive characterizations and density functional theory calculations reveal that Phe modification shifts down the d -band center of δ-MnO 2 from −1.22 eV to −2.78 eV, and attenuates the oxidation state of Mn species. This unique configuration suppresses the over-oxidation of NH 3 and weakens the interaction between catalyst and poisons, while promoting the activation of NH 3 to *NH 2 with a lower energy barrier, thus driving a desired Eley-Rideal pathway and endowing strong tolerance against SO 2 and H 2 O at low temperatures. This work provides a novel organic-inorganic hybrid strategy for designing robust low-temperature NH 3 -SCR catalysts with superior activity and poison resistance.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Fan Wu,Junyao Li,Ning Gu,Wenxin Huang,Datong Wu,Wenrong Cai,Zheng-Zhi Yin,Yong Kong
Since d -glucose (D-Glu) plays significant roles in energy generation and metabolite production while l -glucose (L-Glu) cannot be metabolized, discrimination and detection of Glu enantiomers is of great importance for people's daily life. Herein, chiral Au/Co 3 O 4 nanoclusters are synthesized by using L-cysteine (L-Cys) as the chiral inducer and Au 3+ as the self-assembly inducer. The introduction of Au can also enhance the conductivity of the chiral Au/Co 3 O 4 . The resulting L-Au/Co 3 O 4 nanoclusters show higher affinity toward L-Glu due to the consistent chirality of L-Au/Co 3 O 4 nanoclusters and L-Glu, which can be used for the electrochemical discrimination and detection of Glu enantiomers. Accordingly, the D-Au/Co 3 O 4 nanoclusters synthesized by using D-Cys as the chiral inducer show higher affinity toward D-Glu, and thus they can also be used for the electrochemical discrimination and detection of Glu enantiomers. The chiral Au/Co 3 O 4 exhibits high selectivity, reproducibility and stability when used for the discrimination of Glu enantiomers. Although the linear range is relatively narrow (0.05–1.5 mM), both chiral discrimination and electrochemical detection can be achieved by the proposed dual-functional sensor, which is quite different from the common Glu sensors.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
Huimin Wang,Guangxin Wang,Ruoxi Liu,Jin Yong Lee,Shufeng Liu,Yingqi Tang,Jing Yu
The development of highly efficient chiral recognition systems by simple and cost-effective means is critical to advance the precise application of chiral molecules. Herein, a novel electrochemiluminescence (ECL) enantioselective recognition platform was constructed based on l-histidine-functionalized zeolitic imidazolate frameworks (l-His-ZIF8). Detailed characterization verified the successful preparation of the chiral l-His-ZIF8 material. It was then applied to the chiral discrimination of six amino acids: cysteine (Cys), tryptophan (Trp), phenylalanine (Phe), glutamic acid (Glu), histidine (His), and proline (Pro). Among them, the system employing Ru(bpy)32+ as the luminophore demonstrated the most efficient chiral recognition of d-Cys over l-Cys, as evidenced by a distinct ECL signal ratio (ECLd-Cys/ECLl-Cys) of 3.3 at 1 mM. A detection limit of approximately 0.193 mM was achieved for Cys enantiomers. Conversely, an inverse chiral recognition was observed for the tryptophan (Trp)/sodium tetraphenylborate (TPB) system, resulting in a stronger ECL response for l-Trp over that for d-Trp (ECLl-Trp/ECLd-Trp = 2.4 at 1 mM). Theoretical calculations indicate that the excellent ECL enantioselectivity stems from the preferential binding of the “host” l-His-ZIF8 with specific “guest” enantiomers (d-Cys or l-Trp) via stronger intermolecular interactions. This preferential binding leads to the formation of more stable diastereoisomers that facilitate electrooxidation of more “guests” to generate a stronger luminescent signal than its isomer. This study establishes a novel strategy for electrochemiluminescent enantiomer discrimination using chiral site-engineered metal–organic frameworks (MOFs) and opens new avenues for the future of chiral sensing.
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高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 6
·1-12
·
IF 5.7
Niu Xiaohui,Zhao Rui,Yan Simeng,Li Hongxia,Yang Jing,Cao Kunjie,Liu Xiaoyu,Wang Kunjie
Abstract Chiral materials have drawn the widespread attention for their its chiral recognition ability. The design and synthesis of chiral material are of importance owing to the unpredictability in controlling chirality during the synthesis process. To circumvent problems, a chiral MOF (D-His-ZIF-8) was synthesized by ligand exchange of 2-methylimidazole (Hmim) on ZIF-8 by D-histidine (D-His), which can be treated as chiral host to distinguish amino acid enantiomers. The obtained D-His-ZIF-8 can provide chiral nanochannels for amino acid guests. Meanwhile, polynary transition-metal ion (Co 2+ and Fe 3+ ) coordinating with polydopamine (PDA) wrapped on the surface of D-His-ZIF-8 can increase the active sites. The electrochemical chiral recognition behavior showed that D-His-ZIF-8@CoFe-PDA exhibited good recognition of the tryptophan enantiomer (L/D-Trp) (working potential of −0.2 V vs. Hg/HgCl 2 ). The LOD and LOQ of L-Trp were 0.066 mM and 0.22 mM, respectively, while the LOD and LOQ of D-Trp were 0.15 mM and 0.50 mM, respectively. Finally, the usefulness of D-His-ZIF-8@CoFe-PDA/GCE was evaluated with a recovery of 94.4–103%. The analysis of real samples shows that D-His-ZIF-8@CoFe-PDA/GCE is a feasible sensing platform for the detection of L-Trp and D-Trp. Graphical abstract
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 6
·976-984
·
IF 3.1
Hou Huipeng,Tang Shanshan,Wang Wei,Liu Miao,Liang Axin,Xie Bingteng,Yi Yue,Luo Aiqin
I n this work, an electrochemical chiral sensor of a nanowire-like chiral metal-organic framework/multiwalled carbon nanotube-chitosan/glassy carbon electrode( D -MOF/MWCNTs-CS/GCE) was proposed for the enantiorecognition of L / D -tryptophan( L / D -Trp). The asymmetrical spatial structure of D -MOF provides the feasibility for the enantiorecognition of Trp enantiomers. Moreover, differential pulse voltammetry(DPV) was carried out to be the detection method and the DPV peak potential difference(Δ E p ) between L -Trp and D -Trp was referred as the index of the enantiorecognition performance. Several parameters, such as mass ratios and drop-coated volume of MWCNTs-CS, drop-coated volume and concentration of D -MOF, pH and detection temperature of D -MOF/MWCNTs-CS/GCE were optimized for the largest Δ E p value. And the molecular dynamics(MD) simulation was used to elucidate the enantiorecognition mechanism. Furthermore, the proportions of D -Trp( D -Trp%) in Trp mixtures were detected in a good linear relationship with the DPV peak potentials( E p ), and the proposed electrochemical chiral sensor exhibited good reproducibility, stability and enantiorecognition ability. Additionally, the proposed electrochemical chiral sensor( D -MOF/MWCNTs-CS/GCE) has a good application prospect in the fields of biomedicine, clinical diagnosis, chemical production, pharmaceuticals safety and so on.
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Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Qian Ye,Jin Li,Yinyin Huang,Hanbin Wu,Yilin Li,Bin Yan
Due to their unique properties, the efficient separation of racemic mixtures remains a great challenge. Recently, membrane separation has been demonstrated as a promising chiral separation technique with the advantages of continuous operation, high throughput, and cost effectiveness. In this work, a cyclodextrin-metal-organic framework (CD-MOF) was prepared and then composited with polyvinylidene fluoride (PVDF) polymer by the phase inversion via immersion precipitation method to form a CD-MOF/PVDF composite membrane for chiral amino acid separation for the first time. The composite membranes prepared under different conditions were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) thermogravimetric analysis (TGA)and differential scanning calorimetry (DSC). The effects of preparation conditions including CD-MOF load, polymer concentration, and casting solution temperature on the hydrophilicity, water flux , bovine serum albumin (BSA) rejection and chiral separation performance of the membrane were investigated. It is found that the composite membrane (V-5%−12) prepared with the CD-MOF load of 5%, polymer concentration of 12%, and casting solution of 70 ℃ shows the best performance with the water flux of 466.4 L/m 2 ∙h, BSA rejection rate of 24.3% and stable 100% separation efficiency for phenylalanine (Phe) enantiomers for 4 h. The interaction energies between the CD-MOF and D -Phe and L -Phe were calculated using the Gaussian 09 program package and the chiral separation mechanism of the composite membrane was proposed at the molecular level. Our work demonstrates great development potentials of the CD-MOF/PVDF composite membrane in the chiral separation of drugs.
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